P04279 (SEMG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Semenogelin-1

Alternative name(s):
Semenogelin I
Short name=SGI

Cleaved into the following 3 chains:

1. Alpha-inhibin-92
2. Alpha-inhibin-31
3. Seminal basic protein

Gene names

Name:	SEMG1
Synonyms:	SEMG

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Ref.18 Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone. Ref.18
Subunit structure	Occurs in disulfide-linked complexes which may also contain two less abundant 71- and 76-kDa semenogelin-related polypeptides. Interacts with EPPIN (via C-terminus); Cys-239 is a critical amino acid for both binding to EPPIN. Ref.16 Ref.17
Subcellular location	Secreted.
Tissue specificity	Seminal vesicle.
Post-translational modification	Transglutaminase substrate. Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.
Sequence similarities	Belongs to the semenogelin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Signal
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	insemination Traceable author statement Ref.1. Source: ProtInc sexual reproduction Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Traceable author statement Ref.2. Source: ProtInc secretory granule Inferred from electronic annotation. Source: InterPro
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P04279-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P04279-2)

The sequence of this isoform differs from the canonical sequence as follows:
     312-371: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 462	439	Semenogelin-1		PRO_0000032351
	Peptide	68 – 159	92	Alpha-inhibin-92 Ref.14		PRO_0000032352
	Peptide	108 – 159	52	Seminal basic protein Ref.9		PRO_0000032353
	Peptide	108 – 138	31	Alpha-inhibin-31 Ref.10 Ref.11		PRO_0000032354
Regions
	Repeat	70 – 129	60	3-1
	Repeat	141 – 200	60	2-1
	Repeat	201 – 260	60	2-2
	Repeat	381 – 439	59	3-2
	Region	70 – 439	370	Repeat-rich region By similarity
	Region	164 – 283	120	Interaction with EPPIN
	Region	261 – 380	120	2 X 60 AA tandem repeats, type 1
Amino acid modifications
	Modified residue	24	1	Pyrrolidone carboxylic acid Probable
	Disulfide bond	239		Interchain
Natural variations
	Alternative sequence	312 – 371	60	Missing in isoform 2.		VSP_004385
	Natural variant	58	1	E → G. Corresponds to variant rs11559137 [ dbSNP | Ensembl ].		VAR_053650
	Natural variant	79	1	S → T Less common genetic variant. Ref.3 Ref.8 Corresponds to variant rs2301366 [ dbSNP | Ensembl ].		VAR_005610
	Natural variant	108	1	H → R. Corresponds to variant rs2233884 [ dbSNP | Ensembl ].		VAR_053651
	Natural variant	372	1	R → L. Ref.3 Corresponds to variant rs2233887 [ dbSNP | Ensembl ].		VAR_022679
Experimental info
	Mutagenesis	239	1	C → G: Abrogates binding to EPPIN and do not inhibit spem motility. Ref.18
	Sequence conflict	100	1	L → Q in AAP82463. Ref.3
	Sequence conflict	235 – 237	3	QTS → LRT in AAO20112. Ref.8
	Sequence conflict	235 – 237	3	QTS → LRT in AAO20113. Ref.8
	Sequence conflict	321	1	K → L AA sequence Ref.12
	Sequence conflict	423	1	K → N in CAA87636. Ref.2
	Sequence conflict	423	1	K → N in AAA18168. Ref.2
	Sequence conflict	457	1	R → Q Ref.4
	Sequence conflict	457	1	R → Q Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: 760F48EFCF2FA702	FASTA	462	52,131
	10 20 30 40 50 60 MKPNIIFVLS LLLILEKQAA VMGQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK 70 80 90 100 110 120 GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK 130 140 150 160 170 180 GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG 190 200 210 220 230 240 AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP 250 260 270 280 290 300 AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY 310 320 330 340 350 360 GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS SSTEERRLHY 370 380 390 400 410 420 GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH 430 440 450 460 EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT
	Isoform 2 [UniParc]. Checksum: 4D7F264E7C7FC15D	FASTA	402	45,322
	10 20 30 40 50 60 MKPNIIFVLS LLLILEKQAA VMGQKGGSKG RLPSEFSQFP HGQKGQHYSG QKGKQQTESK 70 80 90 100 110 120 GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL GGSQQLLHNK QEGRDHDKSK 130 140 150 160 170 180 GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP SGKGISSQYS NTEERLWVHG LSKEQTSVSG 190 200 210 220 230 240 AQKGRKQGGS QSSYVLQTEE LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP 250 260 270 280 290 300 AHQDKLQHGS KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY 310 320 330 340 350 360 GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG ENAKGESGQS TNREQDLLSH 370 380 390 400 EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA QHLNNDRNPL FT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Semenogelin, the predominant protein in human semen. Primary structure and identification of closely related proteins in the male accessory sex glands and on the spermatozoa." Lilja H., Abrahamsson P.-A., Lundwall A. J. Biol. Chem. 264:1894-1900(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Gene structure of semenogelin I and II. The predominant proteins in human semen are encoded by two homologous genes on chromosome 20." Ulvsbaeck M., Lazure C., Lilja H., Spurr N.K., Rao V.V., Loeffler C., Hansmann I., Lundwall A. J. Biol. Chem. 267:18080-18084(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood.
[3]	"Evolution of the hominoid semenogelin genes, the major proteins of ejaculated semen." Jensen-Seaman M.I., Li W.-H. J. Mol. Evol. 57:261-270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-79 AND LEU-372.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Prostate.
[8]	"Reduced polymorphism in the chimpanzee semen coagulating protein, semenogelin I." Kingan S.B., Tatar M., Rand D.M. J. Mol. Evol. 57:159-169(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-237 AND 241-449, VARIANT THR-79.
[9]	"Amino acid sequence of the predominant basic protein in human seminal plasma." Lilja H., Jeppsson J.-O. FEBS Lett. 182:181-184(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 108-159.
[10]	"Partial amino acid sequence of a human seminal plasma peptide with inhibin-like activity." Seidah N.G., Ramasharma K., Sairam M.R., Chretien M. FEBS Lett. 167:98-102(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 108-138.
[11]	"Isolation, structure, and synthesis of a human seminal plasma peptide with inhibin-like activity." Ramasharma K., Sairam M.R., Seidah N.G., Chretien M., Manjunath P., Schiller P.W., Yamashiro D., Li C.H. Science 223:1199-1202(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 108-138.
[12]	"Isolation and structure determination of two peptides occurring in human seminal plasma." Schneider K., Kausler W., Tripier D., Jouvenal K., Spiteller G. Biol. Chem. Hoppe-Seyler 370:353-356(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 316-344.
[13]	"Isolation and identification of N-terminally extended forms of 5-oxoprolylglutamylprolinamide (Glp-Glu-Pro-NH2), a thyrotropin-releasing-hormone (TRH)-like peptide present in human semen." Khan Z., Smyth D.G. Eur. J. Biochem. 212:35-40(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 373-397.
[14]	"Human seminal alpha inhibins: isolation, characterization, and structure." Li C.H., Hammonds R.G., Ramasharma K., Chung D. Proc. Natl. Acad. Sci. U.S.A. 82:4041-4044(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 68-159.
[15]	"Semenogelin I: a coagulum forming, multifunctional seminal vesicle protein." Robert M., Gagnon C. Cell. Mol. Life Sci. 55:944-960(1999) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[16]	"Association of eppin with semenogelin on human spermatozoa." Wang Z., Widgren E.E., Sivashanmugam P., O'Rand M.G., Richardson R.T. Biol. Reprod. 72:1064-1070(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EPPIN.
[17]	"Characterization of an eppin protein complex from human semen and spermatozoa." Wang Z., Widgren E.E., Richardson R.T., O'Rand M.G. Biol. Reprod. 77:476-484(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH LTF; CLU AND EPPIN.
[18]	"Analysis of recombinant human semenogelin as an inhibitor of human sperm motility." Mitra A., Richardson R.T., O'Rand M.G. Biol. Reprod. 82:489-496(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-239.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Shackled sperm - Issue 62 of September 2005

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04440 mRNA. Translation: AAB59506.1. Z47556 Genomic DNA. Translation: CAA87636.1. M81650 Genomic DNA. Translation: AAA18168.1. AY256465 Genomic DNA. Translation: AAP82462.1. AY256466 Genomic DNA. Translation: AAP82463.1. AY256467 Genomic DNA. Translation: AAP82464.1. AY256468 Genomic DNA. Translation: AAP82465.1. AY256469 Genomic DNA. Translation: AAP82466.1. BT007177 mRNA. Translation: AAP35841.1. AL049767 Genomic DNA. Translation: CAB53523.1. CH471077 Genomic DNA. Translation: EAW75871.1. BC007096 mRNA. Translation: AAH07096.1. BC055416 mRNA. Translation: AAH55416.1. AY174423 Genomic DNA. Translation: AAO20112.1. AY174424 Genomic DNA. Translation: AAO20113.1. AY174437 Genomic DNA. Translation: AAO20126.1.
IPI	IPI00023020. IPI00414684.
PIR	WTHUB. B43412.
RefSeq	NP_002998.1. NM_003007.3.
UniGene	Hs.1968.
3D structure databases
ProteinModelPortal	P04279.
ModBase	Search...
Protein-protein interaction databases
IntAct	P04279. 2 interactions.
MINT	MINT-2862979.
PTM databases
PhosphoSite	P04279.
Polymorphism databases
DMDM	134426.
Proteomic databases
PaxDb	P04279.
PRIDE	P04279.
Protocols and materials databases
DNASU	6406.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000244069; ENSP00000244069; ENSG00000124233. ENST00000372781; ENSP00000361867; ENSG00000124233.
GeneID	6406.
KEGG	hsa:6406.
UCSC	uc002xni.2. human. uc002xnj.2. human.
Organism-specific databases
CTD	6406.
GeneCards	GC20P043835.
HGNC	HGNC:10742. SEMG1.
HPA	HPA042476.
MIM	182140. gene.
neXtProt	NX_P04279.
PharmGKB	PA35664.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG74001.
HOVERGEN	HBG054194.
InParanoid	P04279.
OMA	QNPNQDQ.
OrthoDB	EOG4WSW9J.
PhylomeDB	P04279.
Enzyme and pathway databases
Reactome	REACT_116125. Disease.
Gene expression databases
ArrayExpress	P04279.
Bgee	P04279.
CleanEx	HS_SEMG1.
Genevestigator	P04279.
GermOnline	ENSG00000124233. Homo sapiens.
Family and domain databases
InterPro	IPR008836. Semenogelin. [Graphical view]
PANTHER	PTHR10547. PTHR10547. 1 hit.
Pfam	PF05474. Semenogelin. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6406.
NextBio	24890.
PMAP-CutDB	P04279.
SOURCE	Search...

Entry information

Entry name

SEMG1_HUMAN

Accession

Primary (citable) accession number: P04279
Secondary accession number(s): Q53ZV0 , Q53ZV1, Q53ZV2, Q6X4I9, Q6Y809, Q6Y822, Q6Y823, Q86U64, Q96QM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 142 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.