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Protein

Semenogelin-1

Gene

SEMG1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.1 Publication
Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.1 Publication

GO - Molecular functioni

  • metal ion binding Source: UniProtKB

GO - Biological processi

  • antibacterial humoral response Source: UniProtKB
  • cellular protein metabolic process Source: Reactome
  • coagulation Source: UniProtKB
  • insemination Source: UniProtKB
  • negative regulation of calcium ion import Source: CACAO
  • negative regulation of flagellated sperm motility Source: UniProtKB
  • positive regulation of serine-type endopeptidase activity Source: UniProtKB
  • protein heterooligomerization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124233-MONOMER.
ReactomeiR-HSA-6803157. Antimicrobial peptides.
R-HSA-977225. Amyloid fiber formation.

Names & Taxonomyi

Protein namesi
Recommended name:
Semenogelin-1
Alternative name(s):
Cancer/testis antigen 103
Semenogelin I
Short name:
SGI
Cleaved into the following 3 chains:
Gene namesi
Name:SEMG1
Synonyms:SEMG
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:10742. SEMG1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular region Source: Reactome
  • extracellular space Source: UniProtKB
  • nucleus Source: UniProtKB
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi239C → G: Abrogates binding to EPPIN and do not inhibit spem motility. 1 Publication1

Organism-specific databases

DisGeNETi6406.
OpenTargetsiENSG00000124233.
PharmGKBiPA35664.

Polymorphism and mutation databases

BioMutaiSEMG1.
DMDMi134426.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000003235124 – 462Semenogelin-1Add BLAST439
PeptideiPRO_000003235268 – 159Alpha-inhibin-92Add BLAST92
PeptideiPRO_0000032353108 – 159Seminal basic proteinAdd BLAST52
PeptideiPRO_0000032354108 – 138Alpha-inhibin-31Add BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Pyrrolidone carboxylic acidCurated1
Disulfide bondi239Interchain

Post-translational modificationi

Transglutaminase substrate.
Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP04279.
PaxDbiP04279.
PeptideAtlasiP04279.
PRIDEiP04279.

PTM databases

iPTMnetiP04279.
PhosphoSitePlusiP04279.

Miscellaneous databases

PMAP-CutDBP04279.

Expressioni

Tissue specificityi

Seminal vesicle.

Gene expression databases

BgeeiENSG00000124233.
CleanExiHS_SEMG1.
GenevisibleiP04279. HS.

Organism-specific databases

HPAiHPA042476.

Interactioni

Subunit structurei

Occurs in disulfide-linked complexes which may also contain two less abundant 71- and 76-kDa semenogelin-related polypeptides. Interacts with EPPIN (via C-terminus); Cys-239 is a critical amino acid for both binding to EPPIN.2 Publications

Protein-protein interaction databases

BioGridi112306. 29 interactors.
IntActiP04279. 6 interactors.
MINTiMINT-2862979.
STRINGi9606.ENSP00000361867.

Structurei

3D structure databases

ProteinModelPortaliP04279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati70 – 1293-1Add BLAST60
Repeati141 – 2002-1Add BLAST60
Repeati201 – 2602-2Add BLAST60
Repeati381 – 4393-2Add BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 439Repeat-rich regionBy similarityAdd BLAST370
Regioni164 – 283Interaction with EPPIN1 PublicationAdd BLAST120
Regioni261 – 3802 X 60 AA tandem repeats, type 1Add BLAST120

Sequence similaritiesi

Belongs to the semenogelin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410KG9B. Eukaryota.
ENOG4110SI0. LUCA.
GeneTreeiENSGT00390000020321.
HOVERGENiHBG054194.
InParanoidiP04279.
OMAiFHVIVIH.
OrthoDBiEOG091G03E6.
PhylomeDBiP04279.
TreeFamiTF342360.

Family and domain databases

InterProiIPR008836. Semenogelin.
[Graphical view]
PfamiPF05474. Semenogelin. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04279-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKPNIIFVLS LLLILEKQAA VMGQKGGSKG RLPSEFSQFP HGQKGQHYSG
60 70 80 90 100
QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL
110 120 130 140 150
GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP
160 170 180 190 200
SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE
210 220 230 240 250
LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS
260 270 280 290 300
KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY
310 320 330 340 350
GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS
360 370 380 390 400
SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG
410 420 430 440 450
ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA
460
QHLNNDRNPL FT
Length:462
Mass (Da):52,131
Last modified:January 1, 1990 - v2
Checksum:i760F48EFCF2FA702
GO
Isoform 2 (identifier: P04279-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-371: Missing.

Note: No experimental confirmation available.
Show »
Length:402
Mass (Da):45,322
Checksum:i4D7F264E7C7FC15D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100L → Q in AAP82463 (PubMed:14629036).Curated1
Sequence conflicti235 – 237QTS → LRT in AAO20112 (PubMed:14562960).Curated3
Sequence conflicti235 – 237QTS → LRT in AAO20113 (PubMed:14562960).Curated3
Sequence conflicti321K → L AA sequence (PubMed:2757795).Curated1
Sequence conflicti423K → N in CAA87636 (PubMed:1517240).Curated1
Sequence conflicti423K → N in AAA18168 (PubMed:1517240).Curated1
Sequence conflicti457R → Q (Ref. 4) Curated1
Sequence conflicti457R → Q (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05365058E → G.Corresponds to variant rs11559137dbSNPEnsembl.1
Natural variantiVAR_00561079S → T Less common genetic variant. 2 PublicationsCorresponds to variant rs2301366dbSNPEnsembl.1
Natural variantiVAR_053651108H → R.Corresponds to variant rs2233884dbSNPEnsembl.1
Natural variantiVAR_022679372R → L.1 PublicationCorresponds to variant rs2233887dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004385312 – 371Missing in isoform 2. 2 PublicationsAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04440 mRNA. Translation: AAB59506.1.
Z47556 Genomic DNA. Translation: CAA87636.1.
M81650 Genomic DNA. Translation: AAA18168.1.
AY256465 Genomic DNA. Translation: AAP82462.1.
AY256466 Genomic DNA. Translation: AAP82463.1.
AY256467 Genomic DNA. Translation: AAP82464.1.
AY256468 Genomic DNA. Translation: AAP82465.1.
AY256469 Genomic DNA. Translation: AAP82466.1.
BT007177 mRNA. Translation: AAP35841.1.
AL049767 Genomic DNA. Translation: CAB53523.1.
CH471077 Genomic DNA. Translation: EAW75871.1.
BC007096 mRNA. Translation: AAH07096.1.
BC055416 mRNA. Translation: AAH55416.1.
AY174423 Genomic DNA. Translation: AAO20112.1.
AY174424 Genomic DNA. Translation: AAO20113.1.
AY174437 Genomic DNA. Translation: AAO20126.1.
CCDSiCCDS13345.1. [P04279-1]
PIRiB43412. WTHUB.
RefSeqiNP_002998.1. NM_003007.4. [P04279-1]
UniGeneiHs.1968.

Genome annotation databases

EnsembliENST00000372781; ENSP00000361867; ENSG00000124233. [P04279-1]
GeneIDi6406.
KEGGihsa:6406.
UCSCiuc002xni.3. human. [P04279-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

Shackled sperm - Issue 62 of September 2005

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04440 mRNA. Translation: AAB59506.1.
Z47556 Genomic DNA. Translation: CAA87636.1.
M81650 Genomic DNA. Translation: AAA18168.1.
AY256465 Genomic DNA. Translation: AAP82462.1.
AY256466 Genomic DNA. Translation: AAP82463.1.
AY256467 Genomic DNA. Translation: AAP82464.1.
AY256468 Genomic DNA. Translation: AAP82465.1.
AY256469 Genomic DNA. Translation: AAP82466.1.
BT007177 mRNA. Translation: AAP35841.1.
AL049767 Genomic DNA. Translation: CAB53523.1.
CH471077 Genomic DNA. Translation: EAW75871.1.
BC007096 mRNA. Translation: AAH07096.1.
BC055416 mRNA. Translation: AAH55416.1.
AY174423 Genomic DNA. Translation: AAO20112.1.
AY174424 Genomic DNA. Translation: AAO20113.1.
AY174437 Genomic DNA. Translation: AAO20126.1.
CCDSiCCDS13345.1. [P04279-1]
PIRiB43412. WTHUB.
RefSeqiNP_002998.1. NM_003007.4. [P04279-1]
UniGeneiHs.1968.

3D structure databases

ProteinModelPortaliP04279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112306. 29 interactors.
IntActiP04279. 6 interactors.
MINTiMINT-2862979.
STRINGi9606.ENSP00000361867.

PTM databases

iPTMnetiP04279.
PhosphoSitePlusiP04279.

Polymorphism and mutation databases

BioMutaiSEMG1.
DMDMi134426.

Proteomic databases

MaxQBiP04279.
PaxDbiP04279.
PeptideAtlasiP04279.
PRIDEiP04279.

Protocols and materials databases

DNASUi6406.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372781; ENSP00000361867; ENSG00000124233. [P04279-1]
GeneIDi6406.
KEGGihsa:6406.
UCSCiuc002xni.3. human. [P04279-1]

Organism-specific databases

CTDi6406.
DisGeNETi6406.
GeneCardsiSEMG1.
HGNCiHGNC:10742. SEMG1.
HPAiHPA042476.
MIMi182140. gene.
neXtProtiNX_P04279.
OpenTargetsiENSG00000124233.
PharmGKBiPA35664.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KG9B. Eukaryota.
ENOG4110SI0. LUCA.
GeneTreeiENSGT00390000020321.
HOVERGENiHBG054194.
InParanoidiP04279.
OMAiFHVIVIH.
OrthoDBiEOG091G03E6.
PhylomeDBiP04279.
TreeFamiTF342360.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124233-MONOMER.
ReactomeiR-HSA-6803157. Antimicrobial peptides.
R-HSA-977225. Amyloid fiber formation.

Miscellaneous databases

GeneWikiiSemenogelin_I.
GenomeRNAii6406.
PMAP-CutDBP04279.
PROiP04279.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124233.
CleanExiHS_SEMG1.
GenevisibleiP04279. HS.

Family and domain databases

InterProiIPR008836. Semenogelin.
[Graphical view]
PfamiPF05474. Semenogelin. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEMG1_HUMAN
AccessioniPrimary (citable) accession number: P04279
Secondary accession number(s): Q53ZV0
, Q53ZV1, Q53ZV2, Q6X4I9, Q6Y809, Q6Y822, Q6Y823, Q86U64, Q96QM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 174 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.