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P04279

- SEMG1_HUMAN

UniProt

P04279 - SEMG1_HUMAN

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Protein
Semenogelin-1
Gene
SEMG1, SEMG
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.1 Publication
Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.1 Publication

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. insemination Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_75925. Amyloids.

Names & Taxonomyi

Protein namesi
Recommended name:
Semenogelin-1
Alternative name(s):
Semenogelin I
Short name:
SGI
Cleaved into the following 3 chains:
Gene namesi
Name:SEMG1
Synonyms:SEMG
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:10742. SEMG1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. extracellular space Source: ProtInc
  3. nucleus Source: UniProt
  4. secretory granule Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi239 – 2391C → G: Abrogates binding to EPPIN and do not inhibit spem motility. 1 Publication

Organism-specific databases

PharmGKBiPA35664.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323
Add
BLAST
Chaini24 – 462439Semenogelin-1
PRO_0000032351Add
BLAST
Peptidei68 – 15992Alpha-inhibin-921 Publication
PRO_0000032352Add
BLAST
Peptidei108 – 15952Seminal basic protein1 Publication
PRO_0000032353Add
BLAST
Peptidei108 – 13831Alpha-inhibin-312 Publications
PRO_0000032354Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Pyrrolidone carboxylic acid Inferred
Disulfide bondi239 – 239Interchain

Post-translational modificationi

Transglutaminase substrate.
Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP04279.
PaxDbiP04279.
PRIDEiP04279.

PTM databases

PhosphoSiteiP04279.

Miscellaneous databases

PMAP-CutDBP04279.

Expressioni

Tissue specificityi

Seminal vesicle.

Gene expression databases

ArrayExpressiP04279.
BgeeiP04279.
CleanExiHS_SEMG1.
GenevestigatoriP04279.

Organism-specific databases

HPAiHPA042476.

Interactioni

Subunit structurei

Occurs in disulfide-linked complexes which may also contain two less abundant 71- and 76-kDa semenogelin-related polypeptides. Interacts with EPPIN (via C-terminus); Cys-239 is a critical amino acid for both binding to EPPIN.2 Publications

Protein-protein interaction databases

BioGridi112306. 7 interactions.
IntActiP04279. 2 interactions.
MINTiMINT-2862979.

Structurei

3D structure databases

ProteinModelPortaliP04279.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati70 – 129603-1
Add
BLAST
Repeati141 – 200602-1
Add
BLAST
Repeati201 – 260602-2
Add
BLAST
Repeati381 – 439593-2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 439370Repeat-rich region By similarity
Add
BLAST
Regioni164 – 283120Interaction with EPPIN
Add
BLAST
Regioni261 – 3801202 X 60 AA tandem repeats, type 1
Add
BLAST

Sequence similaritiesi

Belongs to the semenogelin family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG74001.
HOVERGENiHBG054194.
InParanoidiP04279.
OMAiQNPNQDQ.
OrthoDBiEOG7J4469.
PhylomeDBiP04279.
TreeFamiTF342360.

Family and domain databases

InterProiIPR008836. Semenogelin.
[Graphical view]
PANTHERiPTHR10547. PTHR10547. 1 hit.
PfamiPF05474. Semenogelin. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P04279-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKPNIIFVLS LLLILEKQAA VMGQKGGSKG RLPSEFSQFP HGQKGQHYSG    50
QKGKQQTESK GSFSIQYTYH VDANDHDQSR KSQQYDLNAL HKTTKSQRHL 100
GGSQQLLHNK QEGRDHDKSK GHFHRVVIHH KGGKAHRGTQ NPSQDQGNSP 150
SGKGISSQYS NTEERLWVHG LSKEQTSVSG AQKGRKQGGS QSSYVLQTEE 200
LVANKQQRET KNSHQNKGHY QNVVEVREEH SSKVQTSLCP AHQDKLQHGS 250
KDIFSTQDEL LVYNKNQHQT KNLNQDQQHG RKANKISYQS SSTEERRLHY 300
GENGVQKDVS QSSIYSQTEE KAQGKSQKQI TIPSQEQEHS QKANKISYQS 350
SSTEERRLHY GENGVQKDVS QRSIYSQTEK LVAGKSQIQA PNPKQEPWHG 400
ENAKGESGQS TNREQDLLSH EQKGRHQHGS HGGLDIVIIE QEDDSDRHLA 450
QHLNNDRNPL FT 462
Length:462
Mass (Da):52,131
Last modified:January 1, 1990 - v2
Checksum:i760F48EFCF2FA702
GO
Isoform 2 (identifier: P04279-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-371: Missing.

Note: No experimental confirmation available.

Show »
Length:402
Mass (Da):45,322
Checksum:i4D7F264E7C7FC15D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581E → G.
Corresponds to variant rs11559137 [ dbSNP | Ensembl ].
VAR_053650
Natural varianti79 – 791S → T Less common genetic variant. 2 Publications
Corresponds to variant rs2301366 [ dbSNP | Ensembl ].
VAR_005610
Natural varianti108 – 1081H → R.
Corresponds to variant rs2233884 [ dbSNP | Ensembl ].
VAR_053651
Natural varianti372 – 3721R → L.1 Publication
Corresponds to variant rs2233887 [ dbSNP | Ensembl ].
VAR_022679

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei312 – 37160Missing in isoform 2.
VSP_004385Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001L → Q in AAP82463. 1 Publication
Sequence conflicti235 – 2373QTS → LRT in AAO20112. 1 Publication
Sequence conflicti235 – 2373QTS → LRT in AAO20113. 1 Publication
Sequence conflicti321 – 3211K → L AA sequence 1 Publication
Sequence conflicti423 – 4231K → N in CAA87636. 1 Publication
Sequence conflicti423 – 4231K → N in AAA18168. 1 Publication
Sequence conflicti457 – 4571R → Q1 Publication
Sequence conflicti457 – 4571R → Q1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04440 mRNA. Translation: AAB59506.1.
Z47556 Genomic DNA. Translation: CAA87636.1.
M81650 Genomic DNA. Translation: AAA18168.1.
AY256465 Genomic DNA. Translation: AAP82462.1.
AY256466 Genomic DNA. Translation: AAP82463.1.
AY256467 Genomic DNA. Translation: AAP82464.1.
AY256468 Genomic DNA. Translation: AAP82465.1.
AY256469 Genomic DNA. Translation: AAP82466.1.
BT007177 mRNA. Translation: AAP35841.1.
AL049767 Genomic DNA. Translation: CAB53523.1.
CH471077 Genomic DNA. Translation: EAW75871.1.
BC007096 mRNA. Translation: AAH07096.1.
BC055416 mRNA. Translation: AAH55416.1.
AY174423 Genomic DNA. Translation: AAO20112.1.
AY174424 Genomic DNA. Translation: AAO20113.1.
AY174437 Genomic DNA. Translation: AAO20126.1.
CCDSiCCDS13345.1. [P04279-1]
PIRiB43412. WTHUB.
RefSeqiNP_002998.1. NM_003007.3. [P04279-1]
UniGeneiHs.1968.

Genome annotation databases

EnsembliENST00000244069; ENSP00000244069; ENSG00000124233. [P04279-2]
ENST00000372781; ENSP00000361867; ENSG00000124233. [P04279-1]
GeneIDi6406.
KEGGihsa:6406.
UCSCiuc002xni.2. human. [P04279-1]
uc002xnj.2. human. [P04279-2]

Polymorphism databases

DMDMi134426.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

Shackled sperm - Issue 62 of September 2005

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04440 mRNA. Translation: AAB59506.1 .
Z47556 Genomic DNA. Translation: CAA87636.1 .
M81650 Genomic DNA. Translation: AAA18168.1 .
AY256465 Genomic DNA. Translation: AAP82462.1 .
AY256466 Genomic DNA. Translation: AAP82463.1 .
AY256467 Genomic DNA. Translation: AAP82464.1 .
AY256468 Genomic DNA. Translation: AAP82465.1 .
AY256469 Genomic DNA. Translation: AAP82466.1 .
BT007177 mRNA. Translation: AAP35841.1 .
AL049767 Genomic DNA. Translation: CAB53523.1 .
CH471077 Genomic DNA. Translation: EAW75871.1 .
BC007096 mRNA. Translation: AAH07096.1 .
BC055416 mRNA. Translation: AAH55416.1 .
AY174423 Genomic DNA. Translation: AAO20112.1 .
AY174424 Genomic DNA. Translation: AAO20113.1 .
AY174437 Genomic DNA. Translation: AAO20126.1 .
CCDSi CCDS13345.1. [P04279-1 ]
PIRi B43412. WTHUB.
RefSeqi NP_002998.1. NM_003007.3. [P04279-1 ]
UniGenei Hs.1968.

3D structure databases

ProteinModelPortali P04279.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112306. 7 interactions.
IntActi P04279. 2 interactions.
MINTi MINT-2862979.

PTM databases

PhosphoSitei P04279.

Polymorphism databases

DMDMi 134426.

Proteomic databases

MaxQBi P04279.
PaxDbi P04279.
PRIDEi P04279.

Protocols and materials databases

DNASUi 6406.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000244069 ; ENSP00000244069 ; ENSG00000124233 . [P04279-2 ]
ENST00000372781 ; ENSP00000361867 ; ENSG00000124233 . [P04279-1 ]
GeneIDi 6406.
KEGGi hsa:6406.
UCSCi uc002xni.2. human. [P04279-1 ]
uc002xnj.2. human. [P04279-2 ]

Organism-specific databases

CTDi 6406.
GeneCardsi GC20P043835.
HGNCi HGNC:10742. SEMG1.
HPAi HPA042476.
MIMi 182140. gene.
neXtProti NX_P04279.
PharmGKBi PA35664.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG74001.
HOVERGENi HBG054194.
InParanoidi P04279.
OMAi QNPNQDQ.
OrthoDBi EOG7J4469.
PhylomeDBi P04279.
TreeFami TF342360.

Enzyme and pathway databases

Reactomei REACT_75925. Amyloids.

Miscellaneous databases

GeneWikii Semenogelin_I.
GenomeRNAii 6406.
NextBioi 24890.
PMAP-CutDB P04279.
PROi P04279.
SOURCEi Search...

Gene expression databases

ArrayExpressi P04279.
Bgeei P04279.
CleanExi HS_SEMG1.
Genevestigatori P04279.

Family and domain databases

InterProi IPR008836. Semenogelin.
[Graphical view ]
PANTHERi PTHR10547. PTHR10547. 1 hit.
Pfami PF05474. Semenogelin. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Semenogelin, the predominant protein in human semen. Primary structure and identification of closely related proteins in the male accessory sex glands and on the spermatozoa."
    Lilja H., Abrahamsson P.-A., Lundwall A.
    J. Biol. Chem. 264:1894-1900(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Gene structure of semenogelin I and II. The predominant proteins in human semen are encoded by two homologous genes on chromosome 20."
    Ulvsbaeck M., Lazure C., Lilja H., Spurr N.K., Rao V.V., Loeffler C., Hansmann I., Lundwall A.
    J. Biol. Chem. 267:18080-18084(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Blood.
  3. "Evolution of the hominoid semenogelin genes, the major proteins of ejaculated semen."
    Jensen-Seaman M.I., Li W.-H.
    J. Mol. Evol. 57:261-270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-79 AND LEU-372.
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Prostate.
  8. "Reduced polymorphism in the chimpanzee semen coagulating protein, semenogelin I."
    Kingan S.B., Tatar M., Rand D.M.
    J. Mol. Evol. 57:159-169(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-237 AND 241-449, VARIANT THR-79.
  9. "Amino acid sequence of the predominant basic protein in human seminal plasma."
    Lilja H., Jeppsson J.-O.
    FEBS Lett. 182:181-184(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 108-159.
  10. "Partial amino acid sequence of a human seminal plasma peptide with inhibin-like activity."
    Seidah N.G., Ramasharma K., Sairam M.R., Chretien M.
    FEBS Lett. 167:98-102(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 108-138.
  11. "Isolation, structure, and synthesis of a human seminal plasma peptide with inhibin-like activity."
    Ramasharma K., Sairam M.R., Seidah N.G., Chretien M., Manjunath P., Schiller P.W., Yamashiro D., Li C.H.
    Science 223:1199-1202(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 108-138.
  12. "Isolation and structure determination of two peptides occurring in human seminal plasma."
    Schneider K., Kausler W., Tripier D., Jouvenal K., Spiteller G.
    Biol. Chem. Hoppe-Seyler 370:353-356(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 316-344.
  13. "Isolation and identification of N-terminally extended forms of 5-oxoprolylglutamylprolinamide (Glp-Glu-Pro-NH2), a thyrotropin-releasing-hormone (TRH)-like peptide present in human semen."
    Khan Z., Smyth D.G.
    Eur. J. Biochem. 212:35-40(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 373-397.
  14. "Human seminal alpha inhibins: isolation, characterization, and structure."
    Li C.H., Hammonds R.G., Ramasharma K., Chung D.
    Proc. Natl. Acad. Sci. U.S.A. 82:4041-4044(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 68-159.
  15. "Semenogelin I: a coagulum forming, multifunctional seminal vesicle protein."
    Robert M., Gagnon C.
    Cell. Mol. Life Sci. 55:944-960(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  16. "Association of eppin with semenogelin on human spermatozoa."
    Wang Z., Widgren E.E., Sivashanmugam P., O'Rand M.G., Richardson R.T.
    Biol. Reprod. 72:1064-1070(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EPPIN.
  17. "Characterization of an eppin protein complex from human semen and spermatozoa."
    Wang Z., Widgren E.E., Richardson R.T., O'Rand M.G.
    Biol. Reprod. 77:476-484(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH LTF; CLU AND EPPIN.
  18. "Analysis of recombinant human semenogelin as an inhibitor of human sperm motility."
    Mitra A., Richardson R.T., O'Rand M.G.
    Biol. Reprod. 82:489-496(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-239.

Entry informationi

Entry nameiSEMG1_HUMAN
AccessioniPrimary (citable) accession number: P04279
Secondary accession number(s): Q53ZV0
, Q53ZV1, Q53ZV2, Q6X4I9, Q6Y809, Q6Y822, Q6Y823, Q86U64, Q96QM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 1, 1990
Last modified: September 3, 2014
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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