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Protein

Vitamin D-binding protein

Gene

Gc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.By similarity

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • vitamin D binding Source: RGD
  • vitamin transporter activity Source: InterPro

GO - Biological processi

  • female pregnancy Source: RGD
  • lactation Source: RGD
  • response to estradiol Source: RGD
  • response to nutrient levels Source: RGD
  • response to steroid hormone Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Actin-binding, Vitamin D

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin D-binding protein
Short name:
DBP
Short name:
VDB
Alternative name(s):
Gc-globulin
Group-specific component
Gene namesi
Name:Gc
Synonyms:Dbp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2667. Gc.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cytosol Source: RGD
  • extracellular space Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2163.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Chaini17 – 476460Vitamin D-binding protein1 PublicationPRO_0000001105Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 75PROSITE-ProRule annotation
Disulfide bondi74 ↔ 83PROSITE-ProRule annotation
Disulfide bondi96 ↔ 112PROSITE-ProRule annotation
Disulfide bondi111 ↔ 122PROSITE-ProRule annotation
Disulfide bondi145 ↔ 190PROSITE-ProRule annotation
Disulfide bondi189 ↔ 198PROSITE-ProRule annotation
Disulfide bondi220 ↔ 266PROSITE-ProRule annotation
Disulfide bondi265 ↔ 273PROSITE-ProRule annotation
Disulfide bondi286 ↔ 300PROSITE-ProRule annotation
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence analysis
Disulfide bondi299 ↔ 311PROSITE-ProRule annotation
Disulfide bondi335 ↔ 376PROSITE-ProRule annotation
Disulfide bondi375 ↔ 384PROSITE-ProRule annotation
Disulfide bondi407 ↔ 453PROSITE-ProRule annotation
Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence analysis
Modified residuei434 – 4341PhosphoserineCombined sources
Disulfide bondi452 ↔ 462PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP04276.
PRIDEiP04276.

Interactioni

Subunit structurei

Associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004174.

Chemistry

BindingDBiP04276.

Structurei

3D structure databases

ProteinModelPortaliP04276.
SMRiP04276. Positions 20-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 208192Albumin 1PROSITE-ProRule annotationAdd
BLAST
Domaini209 – 394186Albumin 2PROSITE-ProRule annotationAdd
BLAST
Domaini395 – 47682Albumin 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ALB/AFP/VDB family.PROSITE-ProRule annotation
Contains 3 albumin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410II89. Eukaryota.
ENOG4111PH6. LUCA.
HOGENOMiHOG000140946.
HOVERGENiHBG009729.
InParanoidiP04276.
PhylomeDBiP04276.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
IPR000213. VitD-bd.
IPR015247. VitD-bind_III.
[Graphical view]
PfamiPF00273. Serum_albumin. 2 hits.
PF09164. VitD-bind_III. 1 hit.
[Graphical view]
PRINTSiPR00802. SERUMALBUMIN.
PR00804. VITAMNDBNDNG.
SMARTiSM00103. ALBUMIN. 2 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 1 hit.
PS51438. ALBUMIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVLVLLLA LAFGHALERG RDYEKDKVCQ ELSTLGKDDF RSLSLILYSR
60 70 80 90 100
KFPSSTFEQV SQLVKEVVSL TEECCAEGAD PNCYDTRTSE LSIKSCESDA
110 120 130 140 150
PFPVHPGTSE CCTKEGLERK LCMAALSHQP QEFPAYVEPT NDEICEAFRK
160 170 180 190 200
DPKGFADQFL FEYSSNYGQA PLPLLVGYTK SYLSMVGSCC TSAKPTVCFL
210 220 230 240 250
KERLQMKQLL LLTTMSNRVC SQYAAYGKEK SRMSHLIKLA QKVPTANLED
260 270 280 290 300
VLPLAEDLTE ILSRCCKSTS EDCMARELPE HTLKICGNLS KKNSKFEECC
310 320 330 340 350
YETTPMGIFM CSYFMPTAEP LQLPAIKLPT SKDLCGQSAT QAMDQYTFEL
360 370 380 390 400
SRRTQVPEVF LSKVLDTTLK TLRECCDTQD SVSCFSTQSP LMKRQLTSFI
410 420 430 440 450
EKGQEMCADY SENTFTEYKK KLAERLRTKM PNASPEELAD MVAKHSDFAS
460 470
KCCSINSPPR YCSSQIDAEM RDILQS
Length:476
Mass (Da):53,544
Last modified:May 1, 1992 - v3
Checksum:iD3C7729BC44E221E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321E → Q in AAA41082 (PubMed:2480956).Curated
Sequence conflicti174 – 1741L → P in AAA41080 (PubMed:2419332).Curated
Sequence conflicti210 – 2101L → S in AAA41082 (PubMed:2480956).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12450 mRNA. Translation: AAA41080.1.
J05148 mRNA. Translation: AAA41082.1.
M60205
, M60197, M60198, M60199, M60200, M60201, M60202, M60203, M60204 Genomic DNA. Translation: AAA41081.1.
PIRiA38726. VYRTD.
UniGeneiRn.1437.

Genome annotation databases

UCSCiRGD:2667. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12450 mRNA. Translation: AAA41080.1.
J05148 mRNA. Translation: AAA41082.1.
M60205
, M60197, M60198, M60199, M60200, M60201, M60202, M60203, M60204 Genomic DNA. Translation: AAA41081.1.
PIRiA38726. VYRTD.
UniGeneiRn.1437.

3D structure databases

ProteinModelPortaliP04276.
SMRiP04276. Positions 20-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004174.

Chemistry

BindingDBiP04276.
ChEMBLiCHEMBL2163.

Proteomic databases

PaxDbiP04276.
PRIDEiP04276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2667. rat.

Organism-specific databases

RGDi2667. Gc.

Phylogenomic databases

eggNOGiENOG410II89. Eukaryota.
ENOG4111PH6. LUCA.
HOGENOMiHOG000140946.
HOVERGENiHBG009729.
InParanoidiP04276.
PhylomeDBiP04276.

Miscellaneous databases

NextBioi603153.
PROiP04276.

Family and domain databases

InterProiIPR000264. ALB/AFP/VDB.
IPR020858. Serum_albumin-like.
IPR020857. Serum_albumin_CS.
IPR014760. Serum_albumin_N.
IPR000213. VitD-bd.
IPR015247. VitD-bind_III.
[Graphical view]
PfamiPF00273. Serum_albumin. 2 hits.
PF09164. VitD-bind_III. 1 hit.
[Graphical view]
PRINTSiPR00802. SERUMALBUMIN.
PR00804. VITAMNDBNDNG.
SMARTiSM00103. ALBUMIN. 2 hits.
[Graphical view]
SUPFAMiSSF48552. SSF48552. 3 hits.
PROSITEiPS00212. ALBUMIN_1. 1 hit.
PS51438. ALBUMIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat vitamin D binding protein. Determination of the full-length primary structure from cloned cDNA."
    Cooke N.E.
    J. Biol. Chem. 261:3441-3450(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The vitamin D-binding protein, alpha-fetoprotein, albumin multigene family: detection of transcripts in multiple tissues."
    McLeod J.F., Cooke N.E.
    J. Biol. Chem. 264:21760-21769(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The rat vitamin D binding protein (Gc-globulin) gene. Structural analysis, functional and evolutionary correlations."
    Ray K., Wang X., Zhao M., Cooke N.E.
    J. Biol. Chem. 266:6221-6229(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The amino acid sequence of the NH2-terminal portion of rat and human vitamin D binding protein: evidence for a high degree of homology between rat and human vitamin D binding protein."
    Litwiller R.D., Fass D.N., Kumar R.
    Life Sci. 38:2179-2184(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-40.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-434, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVTDB_RAT
AccessioniPrimary (citable) accession number: P04276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: May 1, 1992
Last modified: November 11, 2015
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.