P04272 (ANXA2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Annexin A2

Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name=PAP-IV
Protein I
p36

Gene names

Name:	ANXA2
Synonyms:	ANX2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
Subunit structure	Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF By similarity. Interacts with COCH By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Melanosome By similarity. Note: In the lamina beneath the plasma membrane.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modification	ISGylated By similarity.
Miscellaneous	It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: Compara cellular response to acid Inferred from electronic annotation. Source: Compara collagen fibril organization Inferred from electronic annotation. Source: Compara fibrinolysis Inferred from electronic annotation. Source: Compara positive regulation of binding Inferred from electronic annotation. Source: Compara positive regulation of vesicle fusion Inferred from electronic annotation. Source: Compara
Cellular_component	Schmidt-Lanterman incisure Inferred from electronic annotation. Source: Compara basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome Inferred from electronic annotation. Source: Compara extrinsic to plasma membrane Inferred from electronic annotation. Source: Compara lipid particle Inferred from electronic annotation. Source: Compara melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell midbody Inferred from electronic annotation. Source: Compara myelin sheath adaxonal region Inferred from electronic annotation. Source: Compara sarcolemma Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 339	338	Annexin A2		PRO_0000067469
Regions
	Repeat	42 – 102	61	Annexin 1
	Repeat	114 – 174	61	Annexin 2
	Repeat	199 – 259	61	Annexin 3
	Repeat	274 – 334	61	Annexin 4
	Region	2 – 24	23	S100A10-binding site Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.4
	Modified residue	24	1	Phosphotyrosine; by SRC By similarity
	Modified residue	26	1	Phosphoserine; by PKC By similarity
Experimental info
	Sequence conflict	149	1	E → G in AAI02517. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P04272 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AA8E2500F4138B3D	FASTA	339	38,612
	10 20 30 40 50 60 MSTVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG VDEVTIVNIL 70 80 90 100 110 120 TNRSNEQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG 130 140 150 160 170 180 LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDIV SDTSGDFRKL MVALAKGRRA 190 200 210 220 230 240 EDGSVIDYEL IDQDARDLYD AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM 250 260 270 280 290 300 LESIKKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM 310 320 330 LKIRSEFKKK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of bovine calpactin I heavy chain (p36), a major cellular substrate for retroviral protein-tyrosine kinases: homology with the human phospholipase A2 inhibitor lipocortin." Kristensen T., Saris C.J.M., Hunter T., Hicks L.J., Noonan D.J., Glenney J.R. Jr., Tack B.F. Biochemistry 25:4497-4503(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[4]	"Association of the S-100-related calpactin I light chain with the NH2-terminal tail of the 36-kDa heavy chain." Glenney J.R. Jr., Boudreau M., Galyean R., Hunter T., Tack B. J. Biol. Chem. 261:10485-10488(1986) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT SER-2.

Web resources

Protein Spotlight

Red velvet - Issue 86 of September 2007

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14056 mRNA. Translation: AAA30421.1. BT021010 mRNA. Translation: AAX09027.1. BC102516 mRNA. Translation: AAI02517.1.
IPI	IPI00706002.
PIR	LUBO36. A03081.
RefSeq	NP_777141.1. NM_174716.1.
UniGene	Bt.4314.
3D structure databases
ProteinModelPortal	P04272.
SMR	P04272. Positions 21-339.
ModBase	Search...
Protein-protein interaction databases
IntAct	P04272. 1 interaction.
Proteomic databases
PaxDb	P04272.
PRIDE	P04272.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000012655; ENSBTAP00000012655; ENSBTAG00000009615.
GeneID	282689.
KEGG	bta:282689.
Organism-specific databases
CTD	302.
Phylogenomic databases
eggNOG	NOG259189.
GeneTree	ENSGT00680000099735.
HOGENOM	HOG000158803.
HOVERGEN	HBG061815.
InParanoid	P04272.
OMA	FIQQDTK.
OrthoDB	EOG4KD6MM.
Family and domain databases
Gene3D	1.10.220.10. 4 hits.
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR002389. AnnexinII. [Graphical view]
PANTHER	PTHR10502. PTHR10502. 1 hit. PTHR10502:SF18. PTHR10502:SF18. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00198. ANNEXINII.
SMART	SM00335. ANX. 4 hits. [Graphical view]
SUPFAM	SSF47874. Annexin. 1 hit.
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806357.

Entry information

Entry name

ANXA2_BOVIN

Accession

Primary (citable) accession number: P04272
Secondary accession number(s): Q3ZCC7, Q5E9B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program