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Protein

Annexin A2

Gene

ANXA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.By similarity

GO - Molecular functioni

  • calcium channel activity Source: AgBase
  • calcium-dependent phospholipid binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • glycoprotein binding Source: AgBase
  • phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
  • phosphatidylserine binding Source: AgBase
  • phospholipase A2 inhibitor activity Source: Ensembl
  • poly(A) RNA binding Source: Ensembl
  • receptor activator activity Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Enzyme and pathway databases

ReactomeiR-BTA-6798695. Neutrophil degranulation.
R-BTA-75205. Dissolution of Fibrin Clot.

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A2
Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name:
PAP-IV
Protein I
p36
Gene namesi
Name:ANXA2
Synonyms:ANX2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: UniProtKB-SubCell
  • basolateral plasma membrane Source: Ensembl
  • cell-cell adherens junction Source: Ensembl
  • cell surface Source: Ensembl
  • cytosol Source: AgBase
  • early endosome Source: Ensembl
  • extracellular exosome Source: Ensembl
  • extracellular membrane-bounded organelle Source: AgBase
  • extracellular space Source: Ensembl
  • extrinsic component of plasma membrane Source: Ensembl
  • intracellular membrane-bounded organelle Source: AgBase
  • late endosome membrane Source: Ensembl
  • lipid particle Source: Ensembl
  • lysosomal membrane Source: Ensembl
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: AgBase
  • membrane-bounded vesicle Source: AgBase
  • membrane raft Source: Ensembl
  • midbody Source: Ensembl
  • myelin sheath adaxonal region Source: Ensembl
  • nucleus Source: AgBase
  • PCSK9-AnxA2 complex Source: Ensembl
  • plasma membrane Source: AgBase
  • sarcolemma Source: Ensembl
  • Schmidt-Lanterman incisure Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000674692 – 339Annexin A2Add BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei24Phosphotyrosine; by SRCBy similarity1
Modified residuei26Phosphoserine; by PKCBy similarity1
Modified residuei49N6-acetyllysine; alternateBy similarity1
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Modified residuei152N6-acetyllysineBy similarity1
Modified residuei184PhosphoserineBy similarity1
Modified residuei199PhosphotyrosineBy similarity1
Modified residuei227N6-acetyllysineBy similarity1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP04272.
PeptideAtlasiP04272.
PRIDEiP04272.

PTM databases

iPTMnetiP04272.

Expressioni

Gene expression databases

BgeeiENSBTAG00000009615.

Interactioni

Subunit structurei

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36 (By similarity). Interacts with ATP1B1 (By similarity). Interacts with DYSF (By similarity). Interacts with COCH. Interacts (via repeat Annexin 1) with PCSK9 (via the C-terminal domain); the interaction inhibits the degradation of LDLR. Interacts with CEACAM1 (via the cytoplasmic domain); this interaction is regulated by phosphorylation of CEACAM1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP04272. 1 interactor.
STRINGi9913.ENSBTAP00000012655.

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 47Combined sources13
Helixi53 – 60Combined sources8
Helixi65 – 79Combined sources15
Helixi83 – 90Combined sources8
Helixi93 – 103Combined sources11
Helixi106 – 117Combined sources12
Helixi125 – 133Combined sources9
Helixi137 – 151Combined sources15
Helixi155 – 162Combined sources8
Helixi165 – 175Combined sources11
Helixi188 – 200Combined sources13
Turni201 – 204Combined sources4
Beta strandi205 – 207Combined sources3
Helixi210 – 219Combined sources10
Helixi222 – 235Combined sources14
Helixi240 – 247Combined sources8
Helixi250 – 264Combined sources15
Helixi266 – 278Combined sources13
Beta strandi279 – 282Combined sources4
Helixi285 – 295Combined sources11
Turni296 – 299Combined sources4
Helixi300 – 311Combined sources12
Helixi315 – 322Combined sources8
Helixi325 – 335Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4X9PX-ray2.01A1-339[»]
ProteinModelPortaliP04272.
SMRiP04272.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati42 – 102Annexin 1Add BLAST61
Repeati114 – 174Annexin 2Add BLAST61
Repeati199 – 259Annexin 3Add BLAST61
Repeati274 – 334Annexin 4Add BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 24S100A10-binding siteSequence analysisAdd BLAST23

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP04272.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG091G0H6H.
TreeFamiTF105452.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF111. PTHR10502:SF111. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG
60 70 80 90 100
VDEVTIVNIL TNRSNEQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL
110 120 130 140 150
GLLKTPAQYD ASELKASMKG LGTDEDSLIE IICSRTNQEL QEINRVYKEM
160 170 180 190 200
YKTDLEKDIV SDTSGDFRKL MVALAKGRRA EDGSVIDYEL IDQDARDLYD
210 220 230 240 250
AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM LESIKKEVKG
260 270 280 290 300
DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM
310 320 330
LKIRSEFKKK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD
Length:339
Mass (Da):38,612
Last modified:January 23, 2007 - v2
Checksum:iAA8E2500F4138B3D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149E → G in AAI02517 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14056 mRNA. Translation: AAA30421.1.
BT021010 mRNA. Translation: AAX09027.1.
BC102516 mRNA. Translation: AAI02517.1.
PIRiA03081. LUBO36.
RefSeqiNP_777141.1. NM_174716.1.
UniGeneiBt.4314.

Genome annotation databases

EnsembliENSBTAT00000012655; ENSBTAP00000012655; ENSBTAG00000009615.
GeneIDi282689.
KEGGibta:282689.

Cross-referencesi

Web resourcesi

Protein Spotlight

Red velvet - Issue 86 of September 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14056 mRNA. Translation: AAA30421.1.
BT021010 mRNA. Translation: AAX09027.1.
BC102516 mRNA. Translation: AAI02517.1.
PIRiA03081. LUBO36.
RefSeqiNP_777141.1. NM_174716.1.
UniGeneiBt.4314.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4X9PX-ray2.01A1-339[»]
ProteinModelPortaliP04272.
SMRiP04272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04272. 1 interactor.
STRINGi9913.ENSBTAP00000012655.

PTM databases

iPTMnetiP04272.

Proteomic databases

PaxDbiP04272.
PeptideAtlasiP04272.
PRIDEiP04272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012655; ENSBTAP00000012655; ENSBTAG00000009615.
GeneIDi282689.
KEGGibta:282689.

Organism-specific databases

CTDi302.

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP04272.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG091G0H6H.
TreeFamiTF105452.

Enzyme and pathway databases

ReactomeiR-BTA-6798695. Neutrophil degranulation.
R-BTA-75205. Dissolution of Fibrin Clot.

Gene expression databases

BgeeiENSBTAG00000009615.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF111. PTHR10502:SF111. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANXA2_BOVIN
AccessioniPrimary (citable) accession number: P04272
Secondary accession number(s): Q3ZCC7, Q5E9B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.