Reviewed, UniProtKB/Swiss-Prot P04272 (ANXA2_BOVIN)

Names and origin

Protein names

Recommended name:
    Annexin A2
Alternative name(s):
    Annexin-2
    Annexin II
    Lipocortin II
    Calpactin I heavy chain
    Chromobindin-8
    p36
    Protein I
    Placental anticoagulant protein IV
      Short name=PAP-IV

Gene names

Name:	ANXA2
Synonyms:	ANX2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
Subunit structure	Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Melanosome By similarity. Note: In the lamina beneath the plasma membrane.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Miscellaneous	It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Cellular component	basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW cytoskeletal protein binding Inferred from electronic annotation. Source: InterPro phospholipase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 339	338	Annexin A2		PRO_0000067469
Regions
	Repeat	42 – 102	61	Annexin 1
	Repeat	114 – 174	61	Annexin 2
	Repeat	199 – 259	61	Annexin 3
	Repeat	274 – 334	61	Annexin 4
	Region	2 – 24	23	S100A10-binding site Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.4
	Modified residue	18	1	Phosphoserine By similarity
	Modified residue	19	1	Phosphothreonine By similarity
	Modified residue	24	1	Phosphotyrosine; by SRC By similarity
	Modified residue	26	1	Phosphoserine; by PKC By similarity
	Modified residue	30	1	Phosphotyrosine By similarity
	Modified residue	104	1	N6-acetyllysine By similarity
	Modified residue	115	1	N6-acetyllysine By similarity
	Modified residue	148	1	N6-acetyllysine By similarity
	Modified residue	152	1	N6-acetyllysine By similarity
	Modified residue	157	1	N6-acetyllysine By similarity
	Modified residue	188	1	Phosphotyrosine By similarity
	Modified residue	199	1	Phosphotyrosine By similarity
	Modified residue	227	1	N6-acetyllysine By similarity
	Modified residue	238	1	Phosphotyrosine By similarity
	Modified residue	275	1	Phosphotyrosine By similarity
	Modified residue	279	1	N6-acetyllysine By similarity
	Modified residue	302	1	N6-acetyllysine By similarity
	Modified residue	313	1	N6-acetyllysine By similarity
	Modified residue	316	1	Phosphotyrosine By similarity
	Modified residue	317	1	Phosphotyrosine By similarity
	Modified residue	318	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	149	1	E → G in AAI02517. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P04272-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AA8E2500F4138B3D	FASTA	339	38,612
	10 20 30 40 50 60 MSTVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG VDEVTIVNIL 70 80 90 100 110 120 TNRSNEQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG 130 140 150 160 170 180 LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDIV SDTSGDFRKL MVALAKGRRA 190 200 210 220 230 240 EDGSVIDYEL IDQDARDLYD AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM 250 260 270 280 290 300 LESIKKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM 310 320 330 LKIRSEFKKK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of bovine calpactin I heavy chain (p36), a major cellular substrate for retroviral protein-tyrosine kinases: homology with the human phospholipase A2 inhibitor lipocortin." Kristensen T., Saris C.J.M., Hunter T., Hicks L.J., Noonan D.J., Glenney J.R. Jr., Tack B.F. Biochemistry 25:4497-4503(1986) [PubMed: 2945590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[4]	"Association of the S-100-related calpactin I light chain with the NH2-terminal tail of the 36-kDa heavy chain." Glenney J.R. Jr., Boudreau M., Galyean R., Hunter T., Tack B. J. Biol. Chem. 261:10485-10488(1986) [PubMed: 2942542] [Abstract] Cited for: ACETYLATION AT SER-2.

Web resources

Protein Spotlight

Red velvet - Issue 86 of September 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14056 mRNA. Translation: AAA30421.1. BT021010 mRNA. Translation: AAX09027.1. BC102516 mRNA. Translation: AAI02517.1.
IPI	IPI00706002.
PIR	LUBO36. A03081.
RefSeq	NP_777141.1.
UniGene	Bt.4314
3D structure databases
SMR	P04272. Positions 21-339.
ModBase	Search...
Protein-protein interaction databases
STRING	P04272.
Genome annotation databases
Ensembl	ENSBTAT00000012655; ENSBTAP00000012655; ENSBTAG00000009615; Bos taurus. [Genome view]
GeneID	282689.
KEGG	bta:282689.
Organism-specific databases
CTD	282689.
Phylogenomic databases
HOVERGEN	P04272.
Family and domain databases
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR002389. AnnexinII. [Graphical view]
Gene3D	G3DSA:1.10.220.10. Annexin. 4 hits.
PANTHER	PTHR10502. Annexin. 1 hit. PTHR10502:SF18. AnnexinII. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00198. ANNEXINII.
SMART	SM00335. ANX. 4 hits. [Graphical view]
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANXA2_BOVIN

Accession

Primary (citable) accession number: P04272
Secondary accession number(s): Q3ZCC7, Q5E9B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)