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Protein

Annexin A2

Gene

ANXA2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.

GO - Molecular functioni

  1. calcium channel activity Source: AgBase
  2. calcium-dependent phospholipid binding Source: UniProtKB-KW
  3. calcium ion binding Source: InterPro
  4. glycoprotein binding Source: AgBase
  5. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
  6. phosphatidylserine binding Source: AgBase
  7. phospholipase A2 inhibitor activity Source: Ensembl
  8. poly(A) RNA binding Source: Ensembl

GO - Biological processi

  1. angiogenesis Source: Ensembl
  2. biomineral tissue development Source: AgBase
  3. calcium ion import Source: AgBase
  4. calcium ion transmembrane transport Source: AgBase
  5. collagen fibril organization Source: Ensembl
  6. fibrinolysis Source: Ensembl
  7. membrane budding Source: Ensembl
  8. membrane raft assembly Source: Ensembl
  9. osteoclast development Source: Ensembl
  10. positive regulation of binding Source: Ensembl
  11. positive regulation of vesicle fusion Source: Ensembl
  12. protein heterotetramerization Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Enzyme and pathway databases

ReactomeiREACT_272543. Dissolution of Fibrin Clot.
REACT_321619. Orphan transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A2
Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name:
PAP-IV
Protein I
p36
Gene namesi
Name:ANXA2
Synonyms:ANX2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 10

Subcellular locationi

  1. Secretedextracellular spaceextracellular matrixbasement membrane
  2. Melanosome By similarity

  3. Note: In the lamina beneath the plasma membrane.

GO - Cellular componenti

  1. basement membrane Source: UniProtKB-SubCell
  2. cell surface Source: Ensembl
  3. cytosol Source: AgBase
  4. early endosome Source: Ensembl
  5. extracellular membrane-bounded organelle Source: AgBase
  6. extracellular space Source: Ensembl
  7. extracellular vesicular exosome Source: Ensembl
  8. extrinsic component of plasma membrane Source: Ensembl
  9. intracellular membrane-bounded organelle Source: AgBase
  10. late endosome membrane Source: Ensembl
  11. lipid particle Source: Ensembl
  12. lysosomal membrane Source: Ensembl
  13. melanosome Source: UniProtKB-SubCell
  14. membrane Source: AgBase
  15. membrane-bounded vesicle Source: AgBase
  16. membrane raft Source: Ensembl
  17. midbody Source: Ensembl
  18. myelin sheath adaxonal region Source: Ensembl
  19. nucleus Source: AgBase
  20. plasma membrane Source: AgBase
  21. sarcolemma Source: Ensembl
  22. Schmidt-Lanterman incisure Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 339338Annexin A2PRO_0000067469Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei24 – 241Phosphotyrosine; by SRCBy similarity
Modified residuei26 – 261Phosphoserine; by PKCBy similarity
Modified residuei49 – 491N6-acetyllysineBy similarity
Modified residuei152 – 1521N6-acetyllysineBy similarity
Modified residuei199 – 1991PhosphotyrosineBy similarity
Modified residuei227 – 2271N6-acetyllysineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP04272.
PRIDEiP04272.

Interactioni

Subunit structurei

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF (By similarity). Interacts with COCH (By similarity).By similarity

Protein-protein interaction databases

IntActiP04272. 1 interaction.

Structurei

Secondary structure

1
339
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 4713Combined sources
Helixi53 – 608Combined sources
Helixi65 – 7915Combined sources
Helixi83 – 908Combined sources
Helixi93 – 10311Combined sources
Helixi106 – 11712Combined sources
Helixi125 – 1339Combined sources
Helixi137 – 15115Combined sources
Helixi155 – 1628Combined sources
Helixi165 – 17511Combined sources
Helixi188 – 20013Combined sources
Turni201 – 2044Combined sources
Beta strandi205 – 2073Combined sources
Helixi210 – 21910Combined sources
Helixi222 – 23514Combined sources
Helixi240 – 2478Combined sources
Helixi250 – 26415Combined sources
Helixi266 – 27813Combined sources
Beta strandi279 – 2824Combined sources
Helixi285 – 29511Combined sources
Turni296 – 2994Combined sources
Helixi300 – 31112Combined sources
Helixi315 – 3228Combined sources
Helixi325 – 33511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4X9PX-ray2.01A1-339[»]
ProteinModelPortaliP04272.
SMRiP04272. Positions 21-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 10261Annexin 1Add
BLAST
Repeati114 – 17461Annexin 2Add
BLAST
Repeati199 – 25961Annexin 3Add
BLAST
Repeati274 – 33461Annexin 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2423S100A10-binding siteSequence AnalysisAdd
BLAST

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiNOG259189.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP04272.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG74XS72.
TreeFamiTF105452.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF18. PTHR10502:SF18. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG
60 70 80 90 100
VDEVTIVNIL TNRSNEQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL
110 120 130 140 150
GLLKTPAQYD ASELKASMKG LGTDEDSLIE IICSRTNQEL QEINRVYKEM
160 170 180 190 200
YKTDLEKDIV SDTSGDFRKL MVALAKGRRA EDGSVIDYEL IDQDARDLYD
210 220 230 240 250
AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM LESIKKEVKG
260 270 280 290 300
DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM
310 320 330
LKIRSEFKKK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD
Length:339
Mass (Da):38,612
Last modified:January 23, 2007 - v2
Checksum:iAA8E2500F4138B3D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491E → G in AAI02517 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14056 mRNA. Translation: AAA30421.1.
BT021010 mRNA. Translation: AAX09027.1.
BC102516 mRNA. Translation: AAI02517.1.
PIRiA03081. LUBO36.
RefSeqiNP_777141.1. NM_174716.1.
XP_010807542.1. XM_010809240.1.
UniGeneiBt.4314.

Genome annotation databases

EnsembliENSBTAT00000012655; ENSBTAP00000012655; ENSBTAG00000009615.
GeneIDi282689.
KEGGibta:282689.

Cross-referencesi

Web resourcesi

Protein Spotlight

Red velvet - Issue 86 of September 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14056 mRNA. Translation: AAA30421.1.
BT021010 mRNA. Translation: AAX09027.1.
BC102516 mRNA. Translation: AAI02517.1.
PIRiA03081. LUBO36.
RefSeqiNP_777141.1. NM_174716.1.
XP_010807542.1. XM_010809240.1.
UniGeneiBt.4314.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4X9PX-ray2.01A1-339[»]
ProteinModelPortaliP04272.
SMRiP04272. Positions 21-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04272. 1 interaction.

Proteomic databases

PaxDbiP04272.
PRIDEiP04272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012655; ENSBTAP00000012655; ENSBTAG00000009615.
GeneIDi282689.
KEGGibta:282689.

Organism-specific databases

CTDi302.

Phylogenomic databases

eggNOGiNOG259189.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiP04272.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG74XS72.
TreeFamiTF105452.

Enzyme and pathway databases

ReactomeiREACT_272543. Dissolution of Fibrin Clot.
REACT_321619. Orphan transporters.

Miscellaneous databases

NextBioi20806357.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF18. PTHR10502:SF18. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of bovine calpactin I heavy chain (p36), a major cellular substrate for retroviral protein-tyrosine kinases: homology with the human phospholipase A2 inhibitor lipocortin."
    Kristensen T., Saris C.J.M., Hunter T., Hicks L.J., Noonan D.J., Glenney J.R. Jr., Tack B.F.
    Biochemistry 25:4497-4503(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  4. "Association of the S-100-related calpactin I light chain with the NH2-terminal tail of the 36-kDa heavy chain."
    Glenney J.R. Jr., Boudreau M., Galyean R., Hunter T., Tack B.
    J. Biol. Chem. 261:10485-10488(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT SER-2.

Entry informationi

Entry nameiANXA2_BOVIN
AccessioniPrimary (citable) accession number: P04272
Secondary accession number(s): Q3ZCC7, Q5E9B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.