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P04271

- S100B_HUMAN

UniProt

P04271 - S100B_HUMAN

Protein

Protein S100-B

Gene

S100B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi19 – 32141; low affinityAdd
    BLAST
    Calcium bindingi62 – 73122; high affinityAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: UniProtKB
    2. calcium ion binding Source: UniProtKB
    3. identical protein binding Source: IntAct
    4. protein binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB
    6. RAGE receptor binding Source: UniProtKB
    7. S100 protein binding Source: UniProtKB
    8. tau protein binding Source: UniProtKB
    9. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. astrocyte differentiation Source: Ensembl
    2. axonogenesis Source: ProtInc
    3. cell proliferation Source: ProtInc
    4. cellular response to hypoxia Source: Ensembl
    5. central nervous system development Source: ProtInc
    6. innate immune response Source: Reactome
    7. learning or memory Source: UniProtKB
    8. long-term synaptic potentiation Source: Ensembl
    9. memory Source: Ensembl
    10. negative regulation of skeletal muscle cell differentiation Source: Ensembl
    11. positive regulation of apoptotic process Source: Ensembl
    12. positive regulation of cell proliferation Source: Ensembl
    13. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    14. regulation of cell shape Source: Ensembl
    15. regulation of neuronal synaptic plasticity Source: Ensembl
    16. response to glucocorticoid Source: Ensembl
    17. response to methylmercury Source: Ensembl

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_116022. Nuclear signaling by ERBB4.
    REACT_118563. RIP-mediated NFkB activation via ZBP1.
    REACT_21281. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
    REACT_24969. TRAF6 mediated NF-kB activation.
    REACT_25195. Advanced glycosylation endproduct receptor signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein S100-B
    Alternative name(s):
    S-100 protein beta chain
    S-100 protein subunit beta
    S100 calcium-binding protein B
    Gene namesi
    Name:S100B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 21

    Organism-specific databases

    HGNCiHGNC:10500. S100B.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. extracellular region Source: Reactome
    3. extracellular space Source: Ensembl
    4. intracellular membrane-bounded organelle Source: HPA
    5. neuronal cell body Source: Ensembl
    6. nucleus Source: UniProtKB
    7. perinuclear region of cytoplasm Source: UniProtKB
    8. ruffle Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34912.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 9291Protein S100-BPRO_0000143966Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21Blocked amino end (Ser)1 Publication
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP04271.
    PeptideAtlasiP04271.
    PRIDEiP04271.

    PTM databases

    PhosphoSiteiP04271.

    Expressioni

    Tissue specificityi

    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

    Gene expression databases

    ArrayExpressiP04271.
    BgeeiP04271.
    CleanExiHS_S100B.
    GenevestigatoriP04271.

    Organism-specific databases

    HPAiCAB000073.
    CAB006825.
    HPA015768.

    Interactioni

    Subunit structurei

    Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with CACYBP in a calcium-dependent manner. Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting. Interacts with S100A6. The S100B dimer interacts with two molecules of CAPZA1. Interacts with AGER. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself13EBI-458391,EBI-458391
    AGERQ151095EBI-458391,EBI-1646426
    MDM2Q009872EBI-458391,EBI-389668
    S100A1P232977EBI-458391,EBI-743686
    S100A11P319495EBI-458391,EBI-701862
    S100A6P067035EBI-458391,EBI-352877
    TP53P046372EBI-458391,EBI-366083

    Protein-protein interaction databases

    BioGridi112193. 18 interactions.
    IntActiP04271. 11 interactions.
    MINTiMINT-192546.
    STRINGi9606.ENSP00000291700.

    Structurei

    Secondary structure

    1
    92
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1917
    Beta strandi22 – 243
    Helixi30 – 4011
    Turni42 – 443
    Beta strandi47 – 493
    Helixi51 – 6111
    Beta strandi65 – 695
    Helixi71 – 8818
    Turni89 – 913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MQ1NMR-A/B2-92[»]
    1UWONMR-A/B2-92[»]
    2H61X-ray1.90A/B/C/D/E/F/G/H1-92[»]
    2M49NMR-B/D2-92[»]
    2PRUNMR-A/B2-92[»]
    3CZTX-ray1.40X1-92[»]
    3D0YX-ray1.50A/B1-92[»]
    3D10X-ray1.65A/B1-92[»]
    3HCMX-ray2.00A/B1-92[»]
    ProteinModelPortaliP04271.
    SMRiP04271. Positions 1-91.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04271.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 4836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini49 – 8436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the S-101 family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG41158.
    HOVERGENiHBG001479.
    OMAiGDAECDF.
    OrthoDBiEOG7R833W.
    PhylomeDBiP04271.
    TreeFamiTF332727.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028481. S100-B.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view]
    PANTHERiPTHR11639:SF17. PTHR11639:SF17. 1 hit.
    PfamiPF01023. S_100. 1 hit.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04271-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE   50
    QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE 92
    Length:92
    Mass (Da):10,713
    Last modified:January 23, 2007 - v2
    Checksum:i43815AC212A3AD6B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59488, M59487 Genomic DNA. Translation: AAA60367.1.
    CR542123 mRNA. Translation: CAG46920.1.
    AP000339 Genomic DNA. No translation available.
    CH471079 Genomic DNA. Translation: EAX09267.1.
    CH471079 Genomic DNA. Translation: EAX09268.1.
    CH471079 Genomic DNA. Translation: EAX09269.1.
    CH471079 Genomic DNA. Translation: EAX09270.1.
    BC001766 mRNA. Translation: AAH01766.1.
    CCDSiCCDS13736.1.
    PIRiA38364. BCHUIB.
    RefSeqiNP_006263.1. NM_006272.2.
    UniGeneiHs.422181.

    Genome annotation databases

    EnsembliENST00000291700; ENSP00000291700; ENSG00000160307.
    ENST00000397648; ENSP00000380769; ENSG00000160307.
    GeneIDi6285.
    KEGGihsa:6285.
    UCSCiuc002zju.1. human.

    Polymorphism databases

    DMDMi134138.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59488 , M59487 Genomic DNA. Translation: AAA60367.1 .
    CR542123 mRNA. Translation: CAG46920.1 .
    AP000339 Genomic DNA. No translation available.
    CH471079 Genomic DNA. Translation: EAX09267.1 .
    CH471079 Genomic DNA. Translation: EAX09268.1 .
    CH471079 Genomic DNA. Translation: EAX09269.1 .
    CH471079 Genomic DNA. Translation: EAX09270.1 .
    BC001766 mRNA. Translation: AAH01766.1 .
    CCDSi CCDS13736.1.
    PIRi A38364. BCHUIB.
    RefSeqi NP_006263.1. NM_006272.2.
    UniGenei Hs.422181.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MQ1 NMR - A/B 2-92 [» ]
    1UWO NMR - A/B 2-92 [» ]
    2H61 X-ray 1.90 A/B/C/D/E/F/G/H 1-92 [» ]
    2M49 NMR - B/D 2-92 [» ]
    2PRU NMR - A/B 2-92 [» ]
    3CZT X-ray 1.40 X 1-92 [» ]
    3D0Y X-ray 1.50 A/B 1-92 [» ]
    3D10 X-ray 1.65 A/B 1-92 [» ]
    3HCM X-ray 2.00 A/B 1-92 [» ]
    ProteinModelPortali P04271.
    SMRi P04271. Positions 1-91.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112193. 18 interactions.
    IntActi P04271. 11 interactions.
    MINTi MINT-192546.
    STRINGi 9606.ENSP00000291700.

    Chemistry

    BindingDBi P04271.
    ChEMBLi CHEMBL4300.

    PTM databases

    PhosphoSitei P04271.

    Polymorphism databases

    DMDMi 134138.

    Proteomic databases

    PaxDbi P04271.
    PeptideAtlasi P04271.
    PRIDEi P04271.

    Protocols and materials databases

    DNASUi 6285.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000291700 ; ENSP00000291700 ; ENSG00000160307 .
    ENST00000397648 ; ENSP00000380769 ; ENSG00000160307 .
    GeneIDi 6285.
    KEGGi hsa:6285.
    UCSCi uc002zju.1. human.

    Organism-specific databases

    CTDi 6285.
    GeneCardsi GC21M048018.
    HGNCi HGNC:10500. S100B.
    HPAi CAB000073.
    CAB006825.
    HPA015768.
    MIMi 176990. gene.
    neXtProti NX_P04271.
    PharmGKBi PA34912.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG41158.
    HOVERGENi HBG001479.
    OMAi GDAECDF.
    OrthoDBi EOG7R833W.
    PhylomeDBi P04271.
    TreeFami TF332727.

    Enzyme and pathway databases

    Reactomei REACT_116022. Nuclear signaling by ERBB4.
    REACT_118563. RIP-mediated NFkB activation via ZBP1.
    REACT_21281. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
    REACT_24969. TRAF6 mediated NF-kB activation.
    REACT_25195. Advanced glycosylation endproduct receptor signaling.

    Miscellaneous databases

    ChiTaRSi S100B. human.
    EvolutionaryTracei P04271.
    GeneWikii S100B.
    GenomeRNAii 6285.
    NextBioi 24405.
    PROi P04271.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04271.
    Bgeei P04271.
    CleanExi HS_S100B.
    Genevestigatori P04271.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028481. S100-B.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view ]
    PANTHERi PTHR11639:SF17. PTHR11639:SF17. 1 hit.
    Pfami PF01023. S_100. 1 hit.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the human S100 beta gene."
      Allore R.J., Friend W.C., O'Hanlon D., Neilson K.M., Baumal R., Dunn R.J., Marks A.
      J. Biol. Chem. 265:15537-15543(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The DNA sequence of human chromosome 21."
      Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
      , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
      Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    6. "Characterization of human brain S100 protein fraction: amino acid sequence of S100 beta."
      Jensen R., Marshak D.R., Anderson C., Lukas T.J., Watterson D.M.
      J. Neurochem. 45:700-705(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-92.
    7. "Purification, characterization and ion binding properties of human brain S100b protein."
      Baudier J., Glasser N., Haglid K., Gerard D.
      Biochim. Biophys. Acta 790:164-173(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: METAL ION-BINDING PROPERTIES.
    8. "Demonstration of heterodimer formation between S100B and S100A6 in the yeast two-hybrid system and human melanoma."
      Yang Q., O'Hanlon D., Heizmann C.W., Marks A.
      Exp. Cell Res. 246:501-509(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH S100A6.
    9. "The 1.5 A crystal structure of human receptor for advanced glycation endproducts (RAGE) ectodomains reveals unique features determining ligand binding."
      Park H., Adsit F.G., Boyington J.C.
      J. Biol. Chem. 285:40762-40770(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AGER.
    10. "The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A."
      Gilquin B., Cannon B.R., Hubstenberger A., Moulouel B., Falk E., Merle N., Assard N., Kieffer S., Rousseau D., Wilder P.T., Weber D.J., Baudier J.
      Mol. Cell. Biol. 30:2724-2736(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATAD3A.
    11. "S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation."
      Yamaguchi F., Umeda Y., Shimamoto S., Tsuchiya M., Tokumitsu H., Tokuda M., Kobayashi R.
      J. Biol. Chem. 287:13787-13798(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PPP5C.
    12. "A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form."
      Smith S.P., Shaw G.S.
      Structure 6:211-222(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    13. "A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex."
      McClintock K.A., Shaw G.S.
      J. Biol. Chem. 278:6251-6257(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR IN COMPLEX WITH CAPZA1 AND CALCIUM.

    Entry informationi

    Entry nameiS100B_HUMAN
    AccessioniPrimary (citable) accession number: P04271
    Secondary accession number(s): D3DSN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 157 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    In addition to metal-ion binding, this protein is involved with the regulation of protein phosphorylation in brain tissue.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 21
      Human chromosome 21: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3