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P04271

- S100B_HUMAN

UniProt

P04271 - S100B_HUMAN

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Protein

Protein S100-B

Gene
S100B
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi19 – 32141; low affinityAdd
BLAST
Calcium bindingi62 – 73122; high affinityAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent protein binding Source: UniProtKB
  2. calcium ion binding Source: UniProtKB
  3. identical protein binding Source: IntAct
  4. protein binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB
  6. RAGE receptor binding Source: UniProtKB
  7. S100 protein binding Source: UniProtKB
  8. tau protein binding Source: UniProtKB
  9. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. astrocyte differentiation Source: Ensembl
  2. axonogenesis Source: ProtInc
  3. cell proliferation Source: ProtInc
  4. cellular response to hypoxia Source: Ensembl
  5. central nervous system development Source: ProtInc
  6. innate immune response Source: Reactome
  7. learning or memory Source: UniProtKB
  8. long-term synaptic potentiation Source: Ensembl
  9. memory Source: Ensembl
  10. negative regulation of skeletal muscle cell differentiation Source: Ensembl
  11. positive regulation of apoptotic process Source: Ensembl
  12. positive regulation of cell proliferation Source: Ensembl
  13. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  14. regulation of cell shape Source: Ensembl
  15. regulation of neuronal synaptic plasticity Source: Ensembl
  16. response to glucocorticoid Source: Ensembl
  17. response to methylmercury Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_116022. Nuclear signaling by ERBB4.
REACT_118563. RIP-mediated NFkB activation via ZBP1.
REACT_21281. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
REACT_24969. TRAF6 mediated NF-kB activation.
REACT_25195. Advanced glycosylation endproduct receptor signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S100-B
Alternative name(s):
S-100 protein beta chain
S-100 protein subunit beta
S100 calcium-binding protein B
Gene namesi
Name:S100B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 21

Organism-specific databases

HGNCiHGNC:10500. S100B.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. extracellular region Source: Reactome
  3. extracellular space Source: Ensembl
  4. intracellular membrane-bounded organelle Source: HPA
  5. neuronal cell body Source: Ensembl
  6. nucleus Source: UniProtKB
  7. perinuclear region of cytoplasm Source: UniProtKB
  8. ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34912.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 9291Protein S100-BPRO_0000143966Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Blocked amino end (Ser)1 Publication
Modified residuei2 – 21N-acetylserine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP04271.
PeptideAtlasiP04271.
PRIDEiP04271.

PTM databases

PhosphoSiteiP04271.

Expressioni

Tissue specificityi

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Gene expression databases

ArrayExpressiP04271.
BgeeiP04271.
CleanExiHS_S100B.
GenevestigatoriP04271.

Organism-specific databases

HPAiCAB000073.
CAB006825.
HPA015768.

Interactioni

Subunit structurei

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with CACYBP in a calcium-dependent manner. Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting. Interacts with S100A6. The S100B dimer interacts with two molecules of CAPZA1. Interacts with AGER. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself13EBI-458391,EBI-458391
AGERQ151095EBI-458391,EBI-1646426
MDM2Q009872EBI-458391,EBI-389668
S100A1P232977EBI-458391,EBI-743686
S100A11P319495EBI-458391,EBI-701862
S100A6P067035EBI-458391,EBI-352877
TP53P046372EBI-458391,EBI-366083

Protein-protein interaction databases

BioGridi112193. 18 interactions.
IntActiP04271. 11 interactions.
MINTiMINT-192546.
STRINGi9606.ENSP00000291700.

Structurei

Secondary structure

1
92
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1917
Beta strandi22 – 243
Helixi30 – 4011
Turni42 – 443
Beta strandi47 – 493
Helixi51 – 6111
Beta strandi65 – 695
Helixi71 – 8818
Turni89 – 913

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MQ1NMR-A/B2-92[»]
1UWONMR-A/B2-92[»]
2H61X-ray1.90A/B/C/D/E/F/G/H1-92[»]
2M49NMR-B/D2-92[»]
2PRUNMR-A/B2-92[»]
3CZTX-ray1.40X1-92[»]
3D0YX-ray1.50A/B1-92[»]
3D10X-ray1.65A/B1-92[»]
3HCMX-ray2.00A/B1-92[»]
ProteinModelPortaliP04271.
SMRiP04271. Positions 1-91.

Miscellaneous databases

EvolutionaryTraceiP04271.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 4836EF-hand 1Add
BLAST
Domaini49 – 8436EF-hand 2Add
BLAST

Sequence similaritiesi

Belongs to the S-101 family.
Contains 2 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG41158.
HOVERGENiHBG001479.
OMAiGDAECDF.
OrthoDBiEOG7R833W.
PhylomeDBiP04271.
TreeFamiTF332727.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028481. S100-B.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]
PANTHERiPTHR11639:SF17. PTHR11639:SF17. 1 hit.
PfamiPF01023. S_100. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04271-1 [UniParc]FASTAAdd to Basket

« Hide

MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE   50
QEVVDKVMET LDNDGDGECD FQEFMAFVAM VTTACHEFFE HE 92
Length:92
Mass (Da):10,713
Last modified:January 23, 2007 - v2
Checksum:i43815AC212A3AD6B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59488, M59487 Genomic DNA. Translation: AAA60367.1.
CR542123 mRNA. Translation: CAG46920.1.
AP000339 Genomic DNA. No translation available.
CH471079 Genomic DNA. Translation: EAX09267.1.
CH471079 Genomic DNA. Translation: EAX09268.1.
CH471079 Genomic DNA. Translation: EAX09269.1.
CH471079 Genomic DNA. Translation: EAX09270.1.
BC001766 mRNA. Translation: AAH01766.1.
CCDSiCCDS13736.1.
PIRiA38364. BCHUIB.
RefSeqiNP_006263.1. NM_006272.2.
UniGeneiHs.422181.

Genome annotation databases

EnsembliENST00000291700; ENSP00000291700; ENSG00000160307.
ENST00000397648; ENSP00000380769; ENSG00000160307.
GeneIDi6285.
KEGGihsa:6285.
UCSCiuc002zju.1. human.

Polymorphism databases

DMDMi134138.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59488 , M59487 Genomic DNA. Translation: AAA60367.1 .
CR542123 mRNA. Translation: CAG46920.1 .
AP000339 Genomic DNA. No translation available.
CH471079 Genomic DNA. Translation: EAX09267.1 .
CH471079 Genomic DNA. Translation: EAX09268.1 .
CH471079 Genomic DNA. Translation: EAX09269.1 .
CH471079 Genomic DNA. Translation: EAX09270.1 .
BC001766 mRNA. Translation: AAH01766.1 .
CCDSi CCDS13736.1.
PIRi A38364. BCHUIB.
RefSeqi NP_006263.1. NM_006272.2.
UniGenei Hs.422181.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MQ1 NMR - A/B 2-92 [» ]
1UWO NMR - A/B 2-92 [» ]
2H61 X-ray 1.90 A/B/C/D/E/F/G/H 1-92 [» ]
2M49 NMR - B/D 2-92 [» ]
2PRU NMR - A/B 2-92 [» ]
3CZT X-ray 1.40 X 1-92 [» ]
3D0Y X-ray 1.50 A/B 1-92 [» ]
3D10 X-ray 1.65 A/B 1-92 [» ]
3HCM X-ray 2.00 A/B 1-92 [» ]
ProteinModelPortali P04271.
SMRi P04271. Positions 1-91.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112193. 18 interactions.
IntActi P04271. 11 interactions.
MINTi MINT-192546.
STRINGi 9606.ENSP00000291700.

Chemistry

BindingDBi P04271.
ChEMBLi CHEMBL4300.

PTM databases

PhosphoSitei P04271.

Polymorphism databases

DMDMi 134138.

Proteomic databases

PaxDbi P04271.
PeptideAtlasi P04271.
PRIDEi P04271.

Protocols and materials databases

DNASUi 6285.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000291700 ; ENSP00000291700 ; ENSG00000160307 .
ENST00000397648 ; ENSP00000380769 ; ENSG00000160307 .
GeneIDi 6285.
KEGGi hsa:6285.
UCSCi uc002zju.1. human.

Organism-specific databases

CTDi 6285.
GeneCardsi GC21M048018.
HGNCi HGNC:10500. S100B.
HPAi CAB000073.
CAB006825.
HPA015768.
MIMi 176990. gene.
neXtProti NX_P04271.
PharmGKBi PA34912.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG41158.
HOVERGENi HBG001479.
OMAi GDAECDF.
OrthoDBi EOG7R833W.
PhylomeDBi P04271.
TreeFami TF332727.

Enzyme and pathway databases

Reactomei REACT_116022. Nuclear signaling by ERBB4.
REACT_118563. RIP-mediated NFkB activation via ZBP1.
REACT_21281. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
REACT_24969. TRAF6 mediated NF-kB activation.
REACT_25195. Advanced glycosylation endproduct receptor signaling.

Miscellaneous databases

ChiTaRSi S100B. human.
EvolutionaryTracei P04271.
GeneWikii S100B.
GenomeRNAii 6285.
NextBioi 24405.
PROi P04271.
SOURCEi Search...

Gene expression databases

ArrayExpressi P04271.
Bgeei P04271.
CleanExi HS_S100B.
Genevestigatori P04271.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028481. S100-B.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view ]
PANTHERi PTHR11639:SF17. PTHR11639:SF17. 1 hit.
Pfami PF01023. S_100. 1 hit.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the human S100 beta gene."
    Allore R.J., Friend W.C., O'Hanlon D., Neilson K.M., Baumal R., Dunn R.J., Marks A.
    J. Biol. Chem. 265:15537-15543(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "The DNA sequence of human chromosome 21."
    Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A.
    , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
    Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  6. "Characterization of human brain S100 protein fraction: amino acid sequence of S100 beta."
    Jensen R., Marshak D.R., Anderson C., Lukas T.J., Watterson D.M.
    J. Neurochem. 45:700-705(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-92.
  7. "Purification, characterization and ion binding properties of human brain S100b protein."
    Baudier J., Glasser N., Haglid K., Gerard D.
    Biochim. Biophys. Acta 790:164-173(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: METAL ION-BINDING PROPERTIES.
  8. "Demonstration of heterodimer formation between S100B and S100A6 in the yeast two-hybrid system and human melanoma."
    Yang Q., O'Hanlon D., Heizmann C.W., Marks A.
    Exp. Cell Res. 246:501-509(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH S100A6.
  9. "The 1.5 A crystal structure of human receptor for advanced glycation endproducts (RAGE) ectodomains reveals unique features determining ligand binding."
    Park H., Adsit F.G., Boyington J.C.
    J. Biol. Chem. 285:40762-40770(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AGER.
  10. "The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A."
    Gilquin B., Cannon B.R., Hubstenberger A., Moulouel B., Falk E., Merle N., Assard N., Kieffer S., Rousseau D., Wilder P.T., Weber D.J., Baudier J.
    Mol. Cell. Biol. 30:2724-2736(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATAD3A.
  11. "S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation."
    Yamaguchi F., Umeda Y., Shimamoto S., Tsuchiya M., Tokumitsu H., Tokuda M., Kobayashi R.
    J. Biol. Chem. 287:13787-13798(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPP5C.
  12. "A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form."
    Smith S.P., Shaw G.S.
    Structure 6:211-222(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  13. "A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex."
    McClintock K.A., Shaw G.S.
    J. Biol. Chem. 278:6251-6257(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR IN COMPLEX WITH CAPZA1 AND CALCIUM.

Entry informationi

Entry nameiS100B_HUMAN
AccessioniPrimary (citable) accession number: P04271
Secondary accession number(s): D3DSN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In addition to metal-ion binding, this protein is involved with the regulation of protein phosphorylation in brain tissue.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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