Reviewed, UniProtKB/Swiss-Prot P04268 (TPM1_CHICK)

Names and origin

Protein names

Recommended name:
    Tropomyosin alpha-1 chain
Alternative name(s):
    Tropomyosin-1
    Alpha-tropomyosin

Gene names

Name:

TPM1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Subunit structure	Heterodimer of an alpha and a beta chain.
Subcellular location	Cytoplasm › cytoskeleton.
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Sequence similarities	Belongs to the tropomyosin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Ligand	Actin-binding
Molecular function	Muscle protein
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P04268-1)

Also known as: Fast-twitch skeletal muscle; CTm7;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P04268-2)

Also known as: Smooth muscle;

The sequence of this isoform differs from the canonical sequence as follows:
     42-80: ELVALQKKLK...LELADKKATD → DIVQLEKQLR...LLFAEENAAK
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLNMHQMLDQTLLELNNM

Isoform 4 (identifier: P04268-4)

Also known as: Fibroblast F1;

The sequence of this isoform differs from the canonical sequence as follows:
     189-212: KCAELEEELKTVTNNLKSLEAQAE → QVRQLEEQLRIMDQTLKALMAAED
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLNMHQMLDQTLLELNNM

Isoform 5 (identifier: P04268-5)

Also known as: Fibroblast F2;

The sequence of this isoform differs from the canonical sequence as follows:
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLNMHQMLDQTLLELNNM

Isoform 6 (identifier: P04268-6)

Also known as: Brain major;

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MDAIKKKMQM...LELADKKATD → MAALSSLEAV...VDQERALREE
     259-284: ELYAQKLKYKAISEELDHALNDMTSI → QLYQQLEQNSRLTNELKLALNED

Isoform 7 (identifier: P04268-7)

Also known as: Brain minor;

The sequence of this isoform differs from the canonical sequence as follows:
     259-284: ELYAQKLKYKAISEELDHALNDMTSI → QLYQQLEQNSRLTNELKLALNED

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 284

284

Tropomyosin alpha-1 chain

PRO_0000205625

Regions

Coiled coil

1 – 284

284

By similarity

Amino acid modifications

Modified residue

1

1

N-acetylmethionine

Natural variations

Alternative sequence

1 – 80

80

MDAIK…KKATD → MAALSSLEAVRKKIRSLQEQ ADAAEERAGKLQREVDQERA LREE in isoform 6.

VSP_006587

Alternative sequence

42 – 80

39

ELVAL…KKATD → DIVQLEKQLRVTEDSRDQVL EELHKSEDSLLFAEENAAK in isoform 2.

VSP_006588

Alternative sequence

189 – 212

24

KCAEL…EAQAE → QVRQLEEQLRIMDQTLKALM AAED in isoform 4.

VSP_006589

Alternative sequence

258 – 284

27

DELYA…DMTSI → EKVAHAKEENLNMHQMLDQT LLELNNM in isoform 2, isoform 4 and isoform 5.

VSP_006590

Alternative sequence

259 – 284

26

ELYAQ…DMTSI → QLYQQLEQNSRLTNELKLAL NED in isoform 6 and isoform 7.

VSP_006591

Natural variant

171

1

I → V

Natural variant

175

1

D → E

Experimental info

Sequence conflict

34

1

E → D AA sequence Ref.1

Sequence conflict

45

1

A → S AA sequence Ref.1

Sequence conflict

63

1

S → A AA sequence Ref.1

Sequence conflict

75

1

D → E AA sequence Ref.1

Sequence conflict

83 – 84

2

SE → AD AA sequence Ref.1

Sequence conflict

132

1

N → S AA sequence Ref.1

Sequence conflict

157

1

E → D AA sequence Ref.1

Sequence conflict

165

1

V → A in AAA48610. Ref.2

Sequence conflict

172

1

I → L AA sequence Ref.1

Sequence conflict

174

1

G → S AA sequence Ref.1

Sequence conflict

188

1

S → G AA sequence Ref.1

Sequence conflict

229

1

T → S AA sequence Ref.1

Secondary structure

1

.

.

.

.

284

Helix Strand Turn

Details...

Helix

3 – 74

72

Helix

75 – 77

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Fast-twitch skeletal muscle) (CTm7) [UniParc]. Last modified August 13, 1987. Version 2. Checksum: DBBBD3DB7F36DACB	FASTA	284	32,766
	10 20 30 40 50 60 MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEERSKQLE DELVALQKKL KGTEDELDKY 70 80 90 100 110 120 SESLKDAQEK LELADKKATD AESEVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ENRAQKDEEK MEIQEIQLKE AKHIAEEADR KYEEVARKLV IIEGDLERAE 190 200 210 220 230 240 ERAELSESKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLTD KLKEAETRAE 250 260 270 280 FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
	Isoform 2 (Smooth muscle). Checksum: C29E05F9DEA8F27F	FASTA	284	32,926
	10 20 30 40 50 60 MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEERSKQLE DDIVQLEKQL RVTEDSRDQV 70 80 90 100 110 120 LEELHKSEDS LLFAEENAAK AESEVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ENRAQKDEEK MEIQEIQLKE AKHIAEEADR KYEEVARKLV IIEGDLERAE 190 200 210 220 230 240 ERAELSESKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLTD KLKEAETRAE 250 260 270 280 FAERSVTKLE KSIDDLEEKV AHAKEENLNM HQMLDQTLLE LNNM
	Isoform 4 (Fibroblast F1). Checksum: E94405DA8D65597F	FASTA	284	32,960
	10 20 30 40 50 60 MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEERSKQLE DELVALQKKL KGTEDELDKY 70 80 90 100 110 120 SESLKDAQEK LELADKKATD AESEVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ENRAQKDEEK MEIQEIQLKE AKHIAEEADR KYEEVARKLV IIEGDLERAE 190 200 210 220 230 240 ERAELSESQV RQLEEQLRIM DQTLKALMAA EDKYSQKEDK YEEEIKVLTD KLKEAETRAE 250 260 270 280 FAERSVTKLE KSIDDLEEKV AHAKEENLNM HQMLDQTLLE LNNM
	Isoform 5 (Fibroblast F2). Checksum: CEA880914631B0DC	FASTA	284	32,821
	10 20 30 40 50 60 MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEERSKQLE DELVALQKKL KGTEDELDKY 70 80 90 100 110 120 SESLKDAQEK LELADKKATD AESEVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ENRAQKDEEK MEIQEIQLKE AKHIAEEADR KYEEVARKLV IIEGDLERAE 190 200 210 220 230 240 ERAELSESKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLTD KLKEAETRAE 250 260 270 280 FAERSVTKLE KSIDDLEEKV AHAKEENLNM HQMLDQTLLE LNNM
	Isoform 6 (Brain major). Checksum: 0F45465ECA0AD748	FASTA	245	28,361
	10 20 30 40 50 60 MAALSSLEAV RKKIRSLQEQ ADAAEERAGK LQREVDQERA LREEAESEVA SLNRRIQLVE 70 80 90 100 110 120 EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAQK DEEKMEIQEI QLKEAKHIAE 130 140 150 160 170 180 EADRKYEEVA RKLVIIEGDL ERAEERAELS ESKCAELEEE LKTVTNNLKS LEAQAEKYSQ 190 200 210 220 230 240 KEDKYEEEIK VLTDKLKEAE TRAEFAERSV TKLEKSIDDL EDQLYQQLEQ NSRLTNELKL ALNED
	Isoform 7 (Brain minor). Checksum: 862297483A4C684C	FASTA	281	32,502
	10 20 30 40 50 60 MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEERSKQLE DELVALQKKL KGTEDELDKY 70 80 90 100 110 120 SESLKDAQEK LELADKKATD AESEVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ENRAQKDEEK MEIQEIQLKE AKHIAEEADR KYEEVARKLV IIEGDLERAE 190 200 210 220 230 240 ERAELSESKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLTD KLKEAETRAE 250 260 270 280 FAERSVTKLE KSIDDLEDQL YQQLEQNSRL TNELKLALNE D

References

[1]	"Amino acid sequence of chicken gizzard gamma-tropomyosin." Lau S.Y.M., Sanders C., Smillie L.B. J. Biol. Chem. 260:7257-7263(1985) [PubMed: 3997866] [Abstract] Cited for: PROTEIN SEQUENCE (ISOFORM 1).
[2]	"Complete nucleotide sequence of the fast-twitch isoform of chicken skeletal muscle alpha-tropomyosin." Gooding C., Reinach F.C., Macleod A.R. Nucleic Acids Res. 15:8105-8105(1987) [PubMed: 3671073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[3]	"The chicken gene encoding the alpha isoform of tropomyosin of fast-twitch muscle fibers: organization, expression and identification of the major proteins synthesized." Lemonnier M., Balvay L., Mouly V., Libri D., Fiszman M.Y. Gene 107:229-240(1991) [PubMed: 1748294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1; 2; 4; 5; 6 AND 7).
[4]	"Differential regulation of skeletal muscle myosin-II and brush border myosin-I enzymology and mechanochemistry by bacterially produced tropomyosin isoforms." Fanning A.S., Wolenski J.S., Mooseker M.S., Izant J.G. Cell Motil. Cytoskeleton 29:29-45(1994) [PubMed: 7820856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 4). Tissue: Body wall.
[5]	"Identification, characterization, and expression of a novel alpha-tropomyosin isoform in cardiac tissues in developing chicken." Denz C.R., Zajdel R.W., Dube S., Dube D.K. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Heart and Skeletal muscle.
[6]	"Distinct alpha-tropomyosin mRNA sequences in chicken skeletal muscle." McLeod A.R. Eur. J. Biochem. 126:293-297(1982) [PubMed: 7128591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-284 (ISOFORM 1).
[7]	"Chick alpha tropomyosin gene contains three sets of mutually exclusive alternatively spliced exons." Lemonnier M., Libri D., Fiszman M.Y. Nucleic Acids Res. 17:5400-5400(1989) [PubMed: 2762137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 189-213 (ISOFORM 4). Tissue: Muscle.
[8]	"Deciphering the design of the tropomyosin molecule." Brown J.H., Kim K.H., Jun G., Greenfield N.J., Dominguez R., Volkmann N., Hitchcock-DeGregori S.E., Cohen C. Proc. Natl. Acad. Sci. U.S.A. 98:8496-8501(2001) [PubMed: 11438684] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-81.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M32441 mRNA. Translation: AAA48610.1.
X57991 , X57993, X57994, X57995 Genomic DNA. Translation: CAA41056.1.
M69144 , M69140, M69142, M69143 Genomic DNA. Translation: AAA48577.1.
X16090 Genomic DNA. Translation: CAA34217.1. Sequence problems.
X57991 , X57993, X57994, X57996 Genomic DNA. Translation: CAA41059.1.
X57991 , X57993, X57994, X57996 Genomic DNA. Translation: CAA41058.1.
X57991 , X57993, X57994, X57996 Genomic DNA. Translation: CAA41057.1.
AY150210 mRNA. Translation: AAN75276.1.
M36337 mRNA. Translation: AAA65121.1.
M36336 mRNA. Translation: AAA65120.1.
J00910 mRNA. Translation: AAA49113.1.

IPI

IPI00572343.
IPI00576859.
IPI00585334.
IPI00598461.
IPI00600961.
IPI00601990.

PIR

TMCHA. S24399.
TMCHS2. S24400.
S24401.
S24402.

RefSeq

NP_990732.1.

UniGene

Gga.4108

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IC2	X-ray	2.00	A/B/C/D	1-80	[»]

SMR

P04268. Positions 1-284.

ModBase

Search...

Protein-protein interaction databases

STRING

P04268.

Genome annotation databases

Ensembl

ENSGALT00000005572; ENSGALP00000005562; ENSGALG00000003521; Gallus gallus. [Genome view]
ENSGALT00000005573; ENSGALP00000005563; ENSGALG00000003521; Gallus gallus. [Genome view]
ENSGALT00000005574; ENSGALP00000005564; ENSGALG00000003521; Gallus gallus. [Genome view]
ENSGALT00000039589; ENSGALP00000038799; ENSGALG00000003521; Gallus gallus. [Genome view]
ENSGALT00000039591; ENSGALP00000038801; ENSGALG00000003521; Gallus gallus. [Genome view]

GeneID

396366.

KEGG

gga:396366.

Organism-specific databases

CTD

396366.

Phylogenomic databases

HOVERGEN

P04268.

OMA

QVEEDIA.

Family and domain databases

InterPro

IPR000533. Tropomyosin.
[Graphical view]

Pfam

PF00261. Tropomyosin. 1 hit.
[Graphical view]

PRINTS

PR00194. TROPOMYOSIN.

PROSITE

PS00326. TROPOMYOSIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TPM1_CHICK

Accession

Primary (citable) accession number: P04268
Secondary accession number(s): P02559 , P08942, P18441, P18442, P49436, P49438, P49439, Q540N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	November 3, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)