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Protein

Keratin, type II cytoskeletal 6B

Gene

KRT6B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei414 – 4141Stutter

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: ProtInc

GO - Biological processi

  • ectoderm development Source: ProtInc
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 6B
Alternative name(s):
Cytokeratin-6B
Short name:
CK-6B
Keratin-6B
Short name:
K6B
Type-II keratin Kb10
Gene namesi
Name:KRT6B
Synonyms:K6B, KRTL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:6444. KRT6B.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • keratin filament Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Involvement in diseasei

Pachyonychia congenita 4 (PC4)3 Publications

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionAn autosomal dominant genodermatosis characterized by hypertrophic nail dystrophy, painful and highly debilitating plantar keratoderma, oral leukokeratosis, and a variety of epidermal cysts.

See also OMIM:615728
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti472 – 4721E → K in PC4. 3 Publications
Corresponds to variant rs60627726 [ dbSNP | Ensembl ].
VAR_023062

Keywords - Diseasei

Disease mutation, Ectodermal dysplasia, Palmoplantar keratoderma

Organism-specific databases

MIMi615728. phenotype.
Orphaneti2309. Pachyonychia congenita.
PharmGKBiPA30232.

Protein family/group databases

Allergomei415. Hom s 5.

Polymorphism and mutation databases

BioMutaiKRT6B.
DMDMi238054404.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 564563Keratin, type II cytoskeletal 6BPRO_0000063732Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei60 – 601PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04259.
PRIDEiP04259.

PTM databases

PhosphoSiteiP04259.

Expressioni

Tissue specificityi

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

Gene expression databases

BgeeiP04259.
CleanExiHS_KRT6B.
GenevisibleiP04259. HS.

Organism-specific databases

HPAiCAB000130.
HPA045697.

Interactioni

Subunit structurei

Heterodimer of a type I and a type II keratin. KRT6 isomers associate with KRT16 and/or KRT17.

Binary interactionsi

WithEntry#Exp.IntActNotes
GOLGA2Q083793EBI-740907,EBI-618309
KIFC3Q9BVG83EBI-740907,EBI-2125614
KRT13A1A4E93EBI-740907,EBI-10171552
KRT15P190124EBI-740907,EBI-739566
KRT31Q153233EBI-740907,EBI-948001
KRT38O760153EBI-740907,EBI-1047263
NDC80O147773EBI-740907,EBI-715849
TFIP11Q9UBB93EBI-740907,EBI-1105213
TRIM54Q9BYV23EBI-740907,EBI-2130429

Protein-protein interaction databases

BioGridi110052. 29 interactions.
IntActiP04259. 15 interactions.
MINTiMINT-1456452.
STRINGi9606.ENSP00000252252.

Structurei

3D structure databases

ProteinModelPortaliP04259.
SMRiP04259. Positions 160-302, 329-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 162161HeadAdd
BLAST
Regioni163 – 472310RodAdd
BLAST
Regioni163 – 19836Coil 1AAdd
BLAST
Regioni199 – 21719Linker 1Add
BLAST
Regioni218 – 30992Coil 1BAdd
BLAST
Regioni310 – 33324Linker 12Add
BLAST
Regioni334 – 472139Coil 2Add
BLAST
Regioni473 – 56492TailAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG315845.
GeneTreeiENSGT00760000118796.
HOGENOMiHOG000230976.
HOVERGENiHBG013015.
InParanoidiP04259.
KOiK07605.
OMAiFASFREC.
OrthoDBiEOG7FV3Q8.
PhylomeDBiP04259.
TreeFamiTF317854.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTSTTIRS HSSSRRGFSA NSARLPGVSR SGFSSISVSR SRGSGGLGGA
60 70 80 90 100
CGGAGFGSRS LYGLGGSKRI SIGGGSCAIS GGYGSRAGGS YGFGGAGSGF
110 120 130 140 150
GFGGGAGIGF GLGGGAGLAG GFGGPGFPVC PPGGIQEVTV NQSLLTPLNL
160 170 180 190 200
QIDPAIQRVR AEEREQIKTL NNKFASFIDK VRFLEQQNKV LDTKWTLLQE
210 220 230 240 250
QGTKTVRQNL EPLFEQYINN LRRQLDNIVG ERGRLDSELR NMQDLVEDLK
260 270 280 290 300
NKYEDEINKR TAAENEFVTL KKDVDAAYMN KVELQAKADT LTDEINFLRA
310 320 330 340 350
LYDAELSQMQ THISDTSVVL SMDNNRNLDL DSIIAEVKAQ YEEIAQRSRA
360 370 380 390 400
EAESWYQTKY EELQITAGRH GDDLRNTKQE IAEINRMIQR LRSEIDHVKK
410 420 430 440 450
QCANLQAAIA DAEQRGEMAL KDAKNKLEGL EDALQKAKQD LARLLKEYQE
460 470 480 490 500
LMNVKLALDV EIATYRKLLE GEECRLNGEG VGQVNISVVQ STVSSGYGGA
510 520 530 540 550
SGVGSGLGLG GGSSYSYGSG LGVGGGFSSS SGRATGGGLS SVGGGSSTIK
560
YTTTSSSSRK SYKH
Length:564
Mass (Da):60,067
Last modified:May 26, 2009 - v5
Checksum:iFFA4C351FBF09F09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 902GS → AG in AAA59466 (PubMed:2410904).Curated
Sequence conflicti116 – 1172AG → PA in AAA59466 (PubMed:2410904).Curated
Sequence conflicti119 – 1213AGG → LC in AAA59466 (PubMed:2410904).Curated
Sequence conflicti159 – 1602VR → IG in AAA59466 (PubMed:2410904).Curated
Sequence conflicti255 – 2551D → V in AAA59466 (PubMed:2410904).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti21 – 211N → S.2 Publications
Corresponds to variant rs428894 [ dbSNP | Ensembl ].
VAR_021265
Natural varianti227 – 2271N → S.2 Publications
Corresponds to variant rs652423 [ dbSNP | Ensembl ].
VAR_021266
Natural varianti365 – 3651I → V.2 Publications
Corresponds to variant rs437014 [ dbSNP | Ensembl ].
VAR_021267
Natural varianti472 – 4721E → K in PC4. 3 Publications
Corresponds to variant rs60627726 [ dbSNP | Ensembl ].
VAR_023062

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42592
, L42584, L42585, L42586, L42587, L42588, L42589, L42590 Genomic DNA. Translation: AAC41768.1.
L42612 mRNA. Translation: AAC41771.1.
L00205
, M11229, L00198, L00199, L00200, L00201, L00202, L00203, L00204 Genomic DNA. Translation: AAA59466.1.
AC055736 Genomic DNA. No translation available.
BC034535 mRNA. Translation: AAH34535.1.
CCDSiCCDS8828.1.
PIRiI61767. KRHUEB.
I61771.
RefSeqiNP_005546.2. NM_005555.3.
UniGeneiHs.708950.

Genome annotation databases

EnsembliENST00000252252; ENSP00000252252; ENSG00000185479.
GeneIDi3854.
KEGGihsa:3854.
UCSCiuc001sak.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Human Intermediate Filament Mutation Database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42592
, L42584, L42585, L42586, L42587, L42588, L42589, L42590 Genomic DNA. Translation: AAC41768.1.
L42612 mRNA. Translation: AAC41771.1.
L00205
, M11229, L00198, L00199, L00200, L00201, L00202, L00203, L00204 Genomic DNA. Translation: AAA59466.1.
AC055736 Genomic DNA. No translation available.
BC034535 mRNA. Translation: AAH34535.1.
CCDSiCCDS8828.1.
PIRiI61767. KRHUEB.
I61771.
RefSeqiNP_005546.2. NM_005555.3.
UniGeneiHs.708950.

3D structure databases

ProteinModelPortaliP04259.
SMRiP04259. Positions 160-302, 329-471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110052. 29 interactions.
IntActiP04259. 15 interactions.
MINTiMINT-1456452.
STRINGi9606.ENSP00000252252.

Protein family/group databases

Allergomei415. Hom s 5.

PTM databases

PhosphoSiteiP04259.

Polymorphism and mutation databases

BioMutaiKRT6B.
DMDMi238054404.

Proteomic databases

PaxDbiP04259.
PRIDEiP04259.

Protocols and materials databases

DNASUi3854.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252252; ENSP00000252252; ENSG00000185479.
GeneIDi3854.
KEGGihsa:3854.
UCSCiuc001sak.3. human.

Organism-specific databases

CTDi3854.
GeneCardsiGC12M052840.
GeneReviewsiKRT6B.
HGNCiHGNC:6444. KRT6B.
HPAiCAB000130.
HPA045697.
MIMi148042. gene.
615728. phenotype.
neXtProtiNX_P04259.
Orphaneti2309. Pachyonychia congenita.
PharmGKBiPA30232.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG315845.
GeneTreeiENSGT00760000118796.
HOGENOMiHOG000230976.
HOVERGENiHBG013015.
InParanoidiP04259.
KOiK07605.
OMAiFASFREC.
OrthoDBiEOG7FV3Q8.
PhylomeDBiP04259.
TreeFamiTF317854.

Miscellaneous databases

GenomeRNAii3854.
NextBioi15165.
PROiP04259.
SOURCEiSearch...

Gene expression databases

BgeeiP04259.
CleanExiHS_KRT6B.
GenevisibleiP04259. HS.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of multiple human genes and cDNAs encoding highly related type II keratin 6 isoforms."
    Takahashi K., Paladini R.D., Coulombe P.A.
    J. Biol. Chem. 270:18581-18592(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-21; SER-227 AND VAL-365.
    Tissue: Skin.
  2. "The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes."
    Tyner A.L., Eichman M.J., Fuchs E.
    Proc. Natl. Acad. Sci. U.S.A. 82:4683-4687(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-21; SER-227 AND VAL-365.
  3. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  5. Bienvenut W.V., Vousden K.H., Lukashchuk N.
    Submitted (MAR-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-9; 16-24; 31-40; 43-86; 169-189; 195-204; 208-369; 376-386; 425-436; 456-475 AND 534-550, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Lung carcinoma.
  6. "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
    Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
    Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 195-203 AND 350-356.
    Tissue: Keratinocyte.
  7. "A mutation in human keratin K6b produces a phenocopy of the K17 disorder pachyonychia congenita type 2."
    Smith F.J.D., Jonkman M.F., van Goor H., Coleman C.M., Covello S.P., Uitto J., McLean W.H.I.
    Hum. Mol. Genet. 7:1143-1148(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT PC4 LYS-472.
  8. Cited for: VARIANT PC4 LYS-472.
  9. Cited for: VARIANT PC4 LYS-472.

Entry informationi

Entry nameiK2C6B_HUMAN
AccessioniPrimary (citable) accession number: P04259
Secondary accession number(s): P48669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: May 26, 2009
Last modified: June 24, 2015
This is version 153 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are at least six isoforms of human type II keratin-6 (K6).
There are two types of cytoskeletal and microfibrillar keratin, I (acidic) and II (neutral to basic) (40-55 and 56-70 kDa, respectively).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.