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P04257

- RL2_GEOSE

UniProt

P04257 - RL2_GEOSE

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Protein
50S ribosomal protein L2
Gene
rplB
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity.UniRule annotation

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-HAMAP
  2. structural constituent of ribosome Source: InterPro
  3. transferase activity Source: InterPro

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L2
Short name:
BstL2
Alternative name(s):
L3
Gene namesi
Name:rplB
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

  1. large ribosomal subunit Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 27627550S ribosomal protein L2UniRule annotation
PRO_0000129528Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi76 – 849
Helixi85 – 873
Beta strandi89 – 968
Beta strandi101 – 1055
Beta strandi129 – 1313
Helixi132 – 1343
Beta strandi140 – 1478
Beta strandi152 – 1598
Beta strandi162 – 1687
Beta strandi171 – 1755
Beta strandi181 – 1855
Beta strandi188 – 1936

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ML5electron microscopy14.00d61-233[»]
1RL2X-ray2.30A/B61-197[»]
2B66X-ray5.90D61-197[»]
2B9NX-ray6.76D61-197[»]
2B9PX-ray6.46D61-197[»]
ProteinModelPortaliP04257.
SMRiP04257. Positions 2-273.

Miscellaneous databases

EvolutionaryTraceiP04257.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPiMF_01320_B. Ribosomal_L2_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR01171. rplB_bact. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04257-1 [UniParc]FASTAAdd to Basket

« Hide

MAIKKYKPTS NGRRGMTVLD FSEITTDQPE KSLLAPLKKR AGRNNQGKIT    50
VRHQGGGHKR QYRIIDFKRD KDGIPGRVAT IEYDPNRSAN IALINYADGE 100
KRYIIAPKNL KVGMEIMSGP DADIKIGNAL PLENIPVGTL VHNIELKPGR 150
GGQLVRAAGT SAQVLGKEGK YVIVRLASGE VRMILGKCRA TVGEVGNEQH 200
ELVNIGKAGR ARWLGIRPTV RGSVMNPVDH PHGGGEGKAP IGRKSPMTPW 250
GKPTLGYKTR KKKNKSDKFI IRRRKK 276
Length:276
Mass (Da):30,331
Last modified:January 23, 2007 - v2
Checksum:i871DBC74317C3AAD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401R → K in BAA31210. 1 Publication
Sequence conflicti106 – 1061A → R in BAA31210. 1 Publication
Sequence conflicti163 – 1631Q → L in BAA31210. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB015722 Genomic DNA. Translation: BAA31210.1.
X54994 Genomic DNA. Translation: CAA38737.1.
PIRiA02759. R5BS2F.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB015722 Genomic DNA. Translation: BAA31210.1 .
X54994 Genomic DNA. Translation: CAA38737.1 .
PIRi A02759. R5BS2F.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ML5 electron microscopy 14.00 d 61-233 [» ]
1RL2 X-ray 2.30 A/B 61-197 [» ]
2B66 X-ray 5.90 D 61-197 [» ]
2B9N X-ray 6.76 D 61-197 [» ]
2B9P X-ray 6.46 D 61-197 [» ]
ProteinModelPortali P04257.
SMRi P04257. Positions 2-273.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P04257.

Family and domain databases

Gene3Di 2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPi MF_01320_B. Ribosomal_L2_B.
InterProi IPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view ]
PANTHERi PTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
Pfami PF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMi SSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsi TIGR01171. rplB_bact. 1 hit.
PROSITEi PS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the genes encoding the ribosomal proteins L23 and L2 from the Bacillus stearothermophilus ribosome."
    Kimura M.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequences of Bacillus stearothermophilus ribosomal protein genes: part of the ribosomal S10 operon."
    Kroemer W.J., Hatakeyama T., Kimura M.
    Biol. Chem. Hoppe-Seyler 371:631-636(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-276.
    Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
  3. "The primary structure of ribosomal protein L2 from Bacillus stearothermophilus."
    Kimura M., Kimura J., Watanabe K.
    Eur. J. Biochem. 153:289-297(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-276.
  4. "Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
    Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
    EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 239-247, CROSS-LINKING TO RRNA.
    Strain: 799.
  5. "The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome."
    Nakagawa A., Nakashima T., Taniguchi M., Hosaka H., Kimura M., Tanaka I.
    EMBO J. 18:1459-1467(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 61-202.
  6. "Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit."
    Ban N., Nissen P., Hansen J., Capel M., Moore P.B., Steitz T.A.
    Nature 400:841-847(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 62-197 ONTO THE H.MARISMORTUI 50S RIBOSOME.

Entry informationi

Entry nameiRL2_GEOSE
AccessioniPrimary (citable) accession number: P04257
Secondary accession number(s): O82995
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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