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P04257 (RL2_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L2

Short name=BstL2
Alternative name(s):
L3
Gene names
Name:rplB
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP-Rule MF_01320

Subunit structure

Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.

Sequence similarities

Belongs to the ribosomal protein L2P family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 27627550S ribosomal protein L2 HAMAP-Rule MF_01320
PRO_0000129528

Experimental info

Sequence conflict401R → K in BAA31210. Ref.1
Sequence conflict1061A → R in BAA31210. Ref.1
Sequence conflict1631Q → L in BAA31210. Ref.1

Secondary structure

....................... 276
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04257 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 871DBC74317C3AAD

FASTA27630,331
        10         20         30         40         50         60 
MAIKKYKPTS NGRRGMTVLD FSEITTDQPE KSLLAPLKKR AGRNNQGKIT VRHQGGGHKR 

        70         80         90        100        110        120 
QYRIIDFKRD KDGIPGRVAT IEYDPNRSAN IALINYADGE KRYIIAPKNL KVGMEIMSGP 

       130        140        150        160        170        180 
DADIKIGNAL PLENIPVGTL VHNIELKPGR GGQLVRAAGT SAQVLGKEGK YVIVRLASGE 

       190        200        210        220        230        240 
VRMILGKCRA TVGEVGNEQH ELVNIGKAGR ARWLGIRPTV RGSVMNPVDH PHGGGEGKAP 

       250        260        270 
IGRKSPMTPW GKPTLGYKTR KKKNKSDKFI IRRRKK 

« Hide

References

[1]"Nucleotide sequence of the genes encoding the ribosomal proteins L23 and L2 from the Bacillus stearothermophilus ribosome."
Kimura M.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequences of Bacillus stearothermophilus ribosomal protein genes: part of the ribosomal S10 operon."
Kroemer W.J., Hatakeyama T., Kimura M.
Biol. Chem. Hoppe-Seyler 371:631-636(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-276.
Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
[3]"The primary structure of ribosomal protein L2 from Bacillus stearothermophilus."
Kimura M., Kimura J., Watanabe K.
Eur. J. Biochem. 153:289-297(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-276.
[4]"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 239-247, CROSS-LINKING TO RRNA.
Strain: 799.
[5]"The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome."
Nakagawa A., Nakashima T., Taniguchi M., Hosaka H., Kimura M., Tanaka I.
EMBO J. 18:1459-1467(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 61-202.
[6]"Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit."
Ban N., Nissen P., Hansen J., Capel M., Moore P.B., Steitz T.A.
Nature 400:841-847(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 62-197 ONTO THE H.MARISMORTUI 50S RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015722 Genomic DNA. Translation: BAA31210.1.
X54994 Genomic DNA. Translation: CAA38737.1.
PIRR5BS2F. A02759.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RL2X-ray2.30A/B61-197[»]
2B9PX-ray6.46D61-197[»]
ProteinModelPortalP04257.
SMRP04257. Positions 2-273.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPMF_01320_B. Ribosomal_L2_B.
InterProIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERPTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
PfamPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR01171. rplB_bact. 1 hit.
PROSITEPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP04257.

Entry information

Entry nameRL2_GEOSE
AccessionPrimary (citable) accession number: P04257
Secondary accession number(s): O82995
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references