50S ribosomal protein L2 - Geobacillus stearothermophilus

Contribute

Send feedback

Read comments (?) or add your own

P04257 (RL2_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
50S ribosomal protein L2
Short name=BstL2

Alternative name(s):
L3

Gene names

Name:

rplB

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP MF_01320_B
Subunit structure	Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3

Chain

2 – 276

275

50S ribosomal protein L2 HAMAP MF_01320_B

PRO_0000129528

Experimental info

Sequence conflict

R → K in BAA31210. Ref.1

Sequence conflict

106

A → R in BAA31210. Ref.1

Sequence conflict

163

Q → L in BAA31210. Ref.1

Secondary structure

276

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P04257 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 871DBC74317C3AAD
Show »

FASTA

276

30,331

        10         20         30         40         50         60 
MAIKKYKPTS NGRRGMTVLD FSEITTDQPE KSLLAPLKKR AGRNNQGKIT VRHQGGGHKR 

        70         80         90        100        110        120 
QYRIIDFKRD KDGIPGRVAT IEYDPNRSAN IALINYADGE KRYIIAPKNL KVGMEIMSGP 

       130        140        150        160        170        180 
DADIKIGNAL PLENIPVGTL VHNIELKPGR GGQLVRAAGT SAQVLGKEGK YVIVRLASGE 

       190        200        210        220        230        240 
VRMILGKCRA TVGEVGNEQH ELVNIGKAGR ARWLGIRPTV RGSVMNPVDH PHGGGEGKAP 

       250        260        270 
IGRKSPMTPW GKPTLGYKTR KKKNKSDKFI IRRRKK

« Hide

References

[1]	"Nucleotide sequence of the genes encoding the ribosomal proteins L23 and L2 from the Bacillus stearothermophilus ribosome." Kimura M. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequences of Bacillus stearothermophilus ribosomal protein genes: part of the ribosomal S10 operon." Kroemer W.J., Hatakeyama T., Kimura M. Biol. Chem. Hoppe-Seyler 371:631-636(1990) [PubMed: 2222862] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-276. Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
[3]	"The primary structure of ribosomal protein L2 from Bacillus stearothermophilus." Kimura M., Kimura J., Watanabe K. Eur. J. Biochem. 153:289-297(1985) [PubMed: 3908098] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-276.
[4]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract] Cited for: PROTEIN SEQUENCE OF 239-247, CROSS-LINKING TO RRNA. Strain: 799.
[5]	"The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome." Nakagawa A., Nakashima T., Taniguchi M., Hosaka H., Kimura M., Tanaka I. EMBO J. 18:1459-1467(1999) [PubMed: 10075918] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 61-202.
[6]	"Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit." Ban N., Nissen P., Hansen J., Capel M., Moore P.B., Steitz T.A. Nature 400:841-847(1999) [PubMed: 10476961] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 62-197 ONTO THE H.MARISMORTUI 50S RIBOSOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB015722 Genomic DNA. Translation: BAA31210.1.
X54994 Genomic DNA. Translation: CAA38737.1.

PIR

R5BS2F. A02759.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RL2	X-ray	2.30	A/B	61-197	[»]

ProteinModelPortal

P04257.

SMR

P04257. Positions 2-273.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

HAMAP

MF_01320_B. Ribosomal_L2_B.
[Tree]

InterPro

IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR002171. Ribosomal_L2.
IPR014726. Ribosomal_L2_3.
IPR005880. Ribosomal_L2_bac-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014722. Transl_SH3-like_sub.
IPR008991. Translation_prot_SH3-like.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.
G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.

PANTHER

PTHR13691:SF5. Ribosom_L2_bac. 1 hit.
PTHR13691. Ribosomal_L2. 1 hit.

Pfam

PF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]

PIRSF

PIRSF002158. Ribosomal_L2. 1 hit.

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.
SSF50104. Transl_SH3_like. 1 hit.

TIGRFAMs

TIGR01171. RplB_bact. 1 hit.

PROSITE

PS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RL2_GEOSE

Accession

Primary (citable) accession number: P04257
Secondary accession number(s): O82995

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	January 23, 2007
Last modified:	May 31, 2011
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry information

Relevant documents