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Protein

50S ribosomal protein L2

Gene

rplB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L2UniRule annotation
Short name:
BstL2
Alternative name(s):
L3
Gene namesi
Name:rplBUniRule annotation
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 27627550S ribosomal protein L2PRO_0000129528Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.UniRule annotation

Structurei

Secondary structure

1
276
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi76 – 849Combined sources
Helixi85 – 873Combined sources
Beta strandi89 – 968Combined sources
Beta strandi101 – 1055Combined sources
Beta strandi129 – 1313Combined sources
Helixi132 – 1343Combined sources
Beta strandi140 – 1478Combined sources
Beta strandi152 – 1598Combined sources
Beta strandi162 – 1687Combined sources
Beta strandi171 – 1755Combined sources
Beta strandi181 – 1855Combined sources
Beta strandi188 – 1936Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ML5electron microscopy14.00d61-233[»]
1RL2X-ray2.30A/B61-197[»]
4V4RX-ray5.90D61-197[»]
4V4SX-ray6.76D61-197[»]
4V4TX-ray6.46D61-197[»]
ProteinModelPortaliP04257.
SMRiP04257. Positions 2-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04257.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L2P family.UniRule annotation

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPiMF_01320_B. Ribosomal_L2_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR01171. rplB_bact. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKKYKPTS NGRRGMTVLD FSEITTDQPE KSLLAPLKKR AGRNNQGKIT
60 70 80 90 100
VRHQGGGHKR QYRIIDFKRD KDGIPGRVAT IEYDPNRSAN IALINYADGE
110 120 130 140 150
KRYIIAPKNL KVGMEIMSGP DADIKIGNAL PLENIPVGTL VHNIELKPGR
160 170 180 190 200
GGQLVRAAGT SAQVLGKEGK YVIVRLASGE VRMILGKCRA TVGEVGNEQH
210 220 230 240 250
ELVNIGKAGR ARWLGIRPTV RGSVMNPVDH PHGGGEGKAP IGRKSPMTPW
260 270
GKPTLGYKTR KKKNKSDKFI IRRRKK
Length:276
Mass (Da):30,331
Last modified:January 23, 2007 - v2
Checksum:i871DBC74317C3AAD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401R → K in BAA31210 (Ref. 1) Curated
Sequence conflicti106 – 1061A → R in BAA31210 (Ref. 1) Curated
Sequence conflicti163 – 1631Q → L in BAA31210 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015722 Genomic DNA. Translation: BAA31210.1.
X54994 Genomic DNA. Translation: CAA38737.1.
PIRiA02759. R5BS2F.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015722 Genomic DNA. Translation: BAA31210.1.
X54994 Genomic DNA. Translation: CAA38737.1.
PIRiA02759. R5BS2F.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ML5electron microscopy14.00d61-233[»]
1RL2X-ray2.30A/B61-197[»]
4V4RX-ray5.90D61-197[»]
4V4SX-ray6.76D61-197[»]
4V4TX-ray6.46D61-197[»]
ProteinModelPortaliP04257.
SMRiP04257. Positions 2-273.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP04257.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPiMF_01320_B. Ribosomal_L2_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR01171. rplB_bact. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the genes encoding the ribosomal proteins L23 and L2 from the Bacillus stearothermophilus ribosome."
    Kimura M.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequences of Bacillus stearothermophilus ribosomal protein genes: part of the ribosomal S10 operon."
    Kroemer W.J., Hatakeyama T., Kimura M.
    Biol. Chem. Hoppe-Seyler 371:631-636(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-276.
    Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
  3. "The primary structure of ribosomal protein L2 from Bacillus stearothermophilus."
    Kimura M., Kimura J., Watanabe K.
    Eur. J. Biochem. 153:289-297(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-276.
  4. "Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
    Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
    EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 239-247, CROSS-LINKING TO RRNA.
    Strain: 799.
  5. "The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome."
    Nakagawa A., Nakashima T., Taniguchi M., Hosaka H., Kimura M., Tanaka I.
    EMBO J. 18:1459-1467(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 61-202.
  6. "Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit."
    Ban N., Nissen P., Hansen J., Capel M., Moore P.B., Steitz T.A.
    Nature 400:841-847(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 62-197 ONTO THE H.MARISMORTUI 50S RIBOSOME.

Entry informationi

Entry nameiRL2_GEOSE
AccessioniPrimary (citable) accession number: P04257
Secondary accession number(s): O82995
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.