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Protein

Histone H2B 1

Gene

his-11

more
Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2B 1
Gene namesi
Name:his-11
ORF Names:ZK131.5
AND
Name:his-15
ORF Names:ZK131.9
AND
Name:his-29
ORF Names:F35H10.11
AND
Name:his-34
ORF Names:F17E9.9
AND
Name:his-44
ORF Names:F08G2.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componentsi: Chromosome II, Chromosome IV

Organism-specific databases

WormBaseiF08G2.1; CE07075; WBGene00001918; his-44.
F17E9.9; CE07075; WBGene00001908; his-34.
F35H10.11; CE07075; WBGene00001903; his-29.
ZK131.5; CE07075; WBGene00001885; his-11.
ZK131.9; CE07075; WBGene00001889; his-15.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: WormBase
  • lipid particle Source: UniProtKB
  • nucleosome Source: UniProtKB-KW
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 122121Histone H2B 1PRO_0000071867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091O-linked (GlcNAc)By similarity
Cross-linki117 – 117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination of Lys-117 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation.By similarity
GlcNAcylation at Ser-109 promotes monoubiquitination of Lys-117. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP04255.
PaxDbiP04255.
PRIDEiP04255.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Protein-protein interaction databases

STRINGi6239.ZK131.9.

Structurei

3D structure databases

ProteinModelPortaliP04255.
SMRiP04255. Positions 30-121.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histone H2B family.Curated

Phylogenomic databases

eggNOGiKOG1744. Eukaryota.
ENOG4111NV5. LUCA.
GeneTreeiENSGT00840000129751.
HOGENOMiHOG000231213.
InParanoidiP04255.
KOiK11252.
OMAiRKETYSI.
OrthoDBiEOG72VH8J.
PhylomeDBiP04255.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERiPTHR23428. PTHR23428. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00621. HISTONEH2B.
SMARTiSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00357. HISTONE_H2B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKPSAKGA KKAAKTVTKP KDGKKRRHAR KESYSVYIYR VLKQVHPDTG
60 70 80 90 100
VSSKAMSIMN SFVNDVFERI AAEASRLAHY NKRSTISSRE IQTAVRLILP
110 120
GELAKHAVSE GTKAVTKYTS SK
Length:122
Mass (Da):13,501
Last modified:January 23, 2007 - v4
Checksum:iC7D161DDEB329401
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 282RH → KK AA sequence (PubMed:3753445).Curated
Sequence conflicti27 – 282RH → KK AA sequence (PubMed:2583267).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15633 Genomic DNA. Translation: CAA33642.1.
FO081169 Genomic DNA. Translation: CCD69642.1.
Z81495 Genomic DNA. Translation: CAB04061.1.
Z83245 Genomic DNA. Translation: CAB05830.1.
Z83245 Genomic DNA. Translation: CAB05832.1.
FO081018 Genomic DNA. Translation: CCD68530.1.
PIRiD88357.
D88753.
RefSeqiNP_496888.1. NM_064487.1.
NP_496892.1. NM_064491.6.
NP_496897.1. NM_064496.1.
NP_501403.1. NM_069002.1.
NP_501409.1. NM_069008.1.
UniGeneiCel.14856.
Cel.29184.
Cel.32395.
Cel.32675.

Genome annotation databases

EnsemblMetazoaiF08G2.1; F08G2.1; WBGene00001918.
F17E9.9; F17E9.9; WBGene00001908.
F35H10.11; F35H10.11; WBGene00001903.
ZK131.5; ZK131.5; WBGene00001885.
ZK131.9; ZK131.9; WBGene00001889.
GeneIDi175026.
175029.
175033.
185332.
191679.
KEGGicel:CELE_F08G2.1.
cel:CELE_F17E9.9.
cel:CELE_F35H10.11.
cel:CELE_ZK131.5.
cel:CELE_ZK131.9.
UCSCiZK131.9. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15633 Genomic DNA. Translation: CAA33642.1.
FO081169 Genomic DNA. Translation: CCD69642.1.
Z81495 Genomic DNA. Translation: CAB04061.1.
Z83245 Genomic DNA. Translation: CAB05830.1.
Z83245 Genomic DNA. Translation: CAB05832.1.
FO081018 Genomic DNA. Translation: CCD68530.1.
PIRiD88357.
D88753.
RefSeqiNP_496888.1. NM_064487.1.
NP_496892.1. NM_064491.6.
NP_496897.1. NM_064496.1.
NP_501403.1. NM_069002.1.
NP_501409.1. NM_069008.1.
UniGeneiCel.14856.
Cel.29184.
Cel.32395.
Cel.32675.

3D structure databases

ProteinModelPortaliP04255.
SMRiP04255. Positions 30-121.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZK131.9.

Proteomic databases

EPDiP04255.
PaxDbiP04255.
PRIDEiP04255.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF08G2.1; F08G2.1; WBGene00001918.
F17E9.9; F17E9.9; WBGene00001908.
F35H10.11; F35H10.11; WBGene00001903.
ZK131.5; ZK131.5; WBGene00001885.
ZK131.9; ZK131.9; WBGene00001889.
GeneIDi175026.
175029.
175033.
185332.
191679.
KEGGicel:CELE_F08G2.1.
cel:CELE_F17E9.9.
cel:CELE_F35H10.11.
cel:CELE_ZK131.5.
cel:CELE_ZK131.9.
UCSCiZK131.9. c. elegans.

Organism-specific databases

CTDi175026.
175029.
175033.
185332.
191679.
WormBaseiF08G2.1; CE07075; WBGene00001918; his-44.
F17E9.9; CE07075; WBGene00001908; his-34.
F35H10.11; CE07075; WBGene00001903; his-29.
ZK131.5; CE07075; WBGene00001885; his-11.
ZK131.9; CE07075; WBGene00001889; his-15.

Phylogenomic databases

eggNOGiKOG1744. Eukaryota.
ENOG4111NV5. LUCA.
GeneTreeiENSGT00840000129751.
HOGENOMiHOG000231213.
InParanoidiP04255.
KOiK11252.
OMAiRKETYSI.
OrthoDBiEOG72VH8J.
PhylomeDBiP04255.

Miscellaneous databases

NextBioi886460.
PROiP04255.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000558. Histone_H2B.
[Graphical view]
PANTHERiPTHR23428. PTHR23428. 1 hit.
PfamiPF00125. Histone. 1 hit.
[Graphical view]
PRINTSiPR00621. HISTONEH2B.
SMARTiSM00427. H2B. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00357. HISTONE_H2B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of Caenorhabditis elegans core histone genes. Genes for different histone classes share common flanking sequence elements."
    Roberts S.B., Emmons S.W., Childs G.
    J. Mol. Biol. 206:567-577(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Multiple forms of histone H2B from the nematode Caenorhabditis elegans."
    Vanfleteren J.R., van Bun S.M., Delcambe L.L., van Beeumen J.J.
    Biochem. J. 235:769-773(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-122.
  4. "The histones of Caenorhabditis elegans: no evidence of stage-specific isoforms. An overview."
    Vanfleteren J.R., van Bun S.M., van Beeumen J.J.
    FEBS Lett. 257:233-237(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiH2B1_CAEEL
AccessioniPrimary (citable) accession number: P04255
Secondary accession number(s): O02656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 135 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.