Reviewed, UniProtKB/Swiss-Prot P04252 (BAHG_VITST)

Names and origin

Protein names

Recommended name:
    Bacterial hemoglobin
Alternative name(s):
    Soluble cytochrome O

Gene names

Name:

vhb

Organism

Vitreoscilla stercoraria

Taxonomic identifier

61 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Vitreoscilla

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein functions as a terminal oxidase.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	oxygen transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 146

146

Bacterial hemoglobin

PRO_0000052456

Sites

Metal binding

53

1

Iron (heme distal ligand) By similarity

Metal binding

85

1

Iron (heme proximal ligand) By similarity

Secondary structure

1

.

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.

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.

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.

.

146

Helix Strand Turn

Details...

Helix

4 – 19

16

Helix

21 – 35

15

Helix

37 – 42

6

Helix

57 – 66

10

Helix

67 – 74

8

Helix

75 – 88

14

Helix

92 – 94

3

Helix

95 – 110

16

Helix

111 – 113

3

Helix

116 – 143

28

Sequences

	Sequence		Length	Mass (Da)
	P04252-1 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: D8F96C641E7EF653	FASTA	146	15,774
	10 20 30 40 50 60 MLDQQTINII KATVPVLKEH GVTITTTFYK NLFAKHPEVR PLFDMGRQES LEQPKALAMT 70 80 90 100 110 120 VLAAAQNIEN LPAILPAVKK IAVKHCQAGV AAAHYPIVGQ ELLGAIKEVL GDAATDDILD 130 140 AWGKAYGVIA DVFIQVEADL YAQAVE

References

[1]	"The Vitreoscilla hemoglobin gene: molecular cloning, nucleotide sequence and genetic expression in Escherichia coli." Khosla C., Bailey J.E. Mol. Gen. Genet. 214:158-161(1988) [PubMed: 3067078] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Primary sequence of a dimeric bacterial haemoglobin from Vitreoscilla." Wakabayashi S., Matsubara H., Webster D.A. Nature 322:481-483(1986) [PubMed: 3736670] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Evidence for partial export of Vitreoscilla hemoglobin into the periplasmic space in Escherichia coli. Implications for protein function." Khosla C., Bailey J.E. J. Mol. Biol. 210:79-89(1989) [PubMed: 2685332] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
[4]	"Characterization of the oxygen-dependent promoter of the Vitreoscilla hemoglobin gene in Escherichia coli." Khosla C., Bailey J.E. J. Bacteriol. 171:5995-6004(1989) [PubMed: 2681149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
[5]	"Unusual structure of the oxygen-binding site in the dimeric bacterial hemoglobin from Vitreoscilla sp." Tarricone C., Galizzi A., Coda A., Ascenzi P., Bolognesi M. Structure 5:497-507(1997) [PubMed: 9115439] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L21670 Genomic DNA. Translation: AAA75506.1.
M30794 Genomic DNA. Translation: AAA27585.1.
M27061 Genomic DNA. Translation: AAA27584.1.

PIR

GGZLB. S08307.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VHB	X-ray	1.83	A/B	1-146	[»]
2VHB	X-ray	1.76	A/B	1-146	[»]
3VHB	X-ray	2.10	A/B	1-146	[»]
4VHB	X-ray	1.80	A/B	1-146	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR012292. Globin.
IPR009050. Globin-like.
IPR000971. Globin_subset.
[Graphical view]

Gene3D

G3DSA:1.10.490.10. Globin_related. 1 hit.

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BAHG_VITST

Accession

Primary (citable) accession number: P04252

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	November 24, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)