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Protein

T-cell surface glycoprotein CD3 delta chain

Gene

Cd3d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed:10935641). Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • cell surface receptor signaling pathway Source: InterPro
  • positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  • positive thymic T cell selection Source: UniProtKB
  • protein complex assembly Source: UniProtKB
  • protein homooligomerization Source: MGI
  • T cell activation Source: UniProtKB

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202424. Downstream TCR signaling.
R-MMU-202427. Phosphorylation of CD3 and TCR zeta chains.
R-MMU-202430. Translocation of ZAP-70 to Immunological synapse.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-389948. PD-1 signaling.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 delta chain
Alternative name(s):
T-cell receptor T3 delta chain
CD_antigen: CD3d
Gene namesi
Name:Cd3d
Synonyms:T3d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88331. Cd3d.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 105ExtracellularSequence analysisAdd BLAST84
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Topological domaini127 – 173CytoplasmicSequence analysisAdd BLAST47

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Thymocyte development in mice lacking CD3D is arrested at the double-positive stage with markedly diminished TCR expression. Thymocytes are defective in the induction of ERK pathway upon TCR engagement, whereas activation of other MAP kinases is unaffected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001648922 – 173T-cell surface glycoprotein CD3 delta chainAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 73By similarity
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei149PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei160PhosphotyrosinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP04235.
PaxDbiP04235.
PRIDEiP04235.

PTM databases

iPTMnetiP04235.
PhosphoSitePlusiP04235.
SwissPalmiP04235.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032094.
CleanExiMM_CD3D.
ExpressionAtlasiP04235. baseline and differential.
GenevisibleiP04235. MM.

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP04235.
ELMiP04235.
IntActiP04235. 1 interactor.
STRINGi10090.ENSMUSP00000034602.

Structurei

3D structure databases

ProteinModelPortaliP04235.
SMRiP04235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 166ITAMPROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IWAX. Eukaryota.
ENOG410Y2TD. LUCA.
GeneTreeiENSGT00510000046930.
HOGENOMiHOG000015287.
HOVERGENiHBG005278.
InParanoidiP04235.
KOiK06450.
OMAiMCQSCVE.
OrthoDBiEOG091G0U7X.
PhylomeDBiP04235.
TreeFamiTF335892.

Family and domain databases

InterProiView protein in InterPro
IPR015484. CD3_esu/gsu/dsu.
IPR015485. CD3D.
IPR036179. Ig-like_dom_sf.
IPR032052. Ig_4.
IPR003110. Phos_immunorcpt_sig_ITAM.
PANTHERiPTHR10570. PTHR10570. 1 hit.
PTHR10570:SF5. PTHR10570:SF5. 1 hit.
PfamiView protein in Pfam
PF16680. Ig_4. 1 hit.
PF02189. ITAM. 1 hit.
SMARTiView protein in SMART
SM00077. ITAM. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS51055. ITAM_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHSGILASL ILIAVLPQGS PFKIQVTEYE DKVFVTCNTS VMHLDGTVEG
60 70 80 90 100
WFAKNKTLNL GKGVLDPRGI YLCNGTEQLA KVVSSVQVHY RMCQNCVELD
110 120 130 140 150
SGTMAGVIFI DLIATLLLAL GVYCFAGHET GRPSGAAEVQ ALLKNEQLYQ
160 170
PLRDREDTQY SRLGGNWPRN KKS
Length:173
Mass (Da):19,032
Last modified:March 20, 1987 - v1
Checksum:iEEEC182BDF2AFB6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12729, M12728 Genomic DNA. Translation: AAA37268.1.
M18222 Genomic DNA. Translation: AAA37399.1.
CCDSiCCDS23124.1.
PIRiA02246. RWMSD2.
RefSeqiNP_038515.3. NM_013487.3.
UniGeneiMm.4527.

Genome annotation databases

EnsembliENSMUST00000034602; ENSMUSP00000034602; ENSMUSG00000032094.
GeneIDi12500.
KEGGimmu:12500.
UCSCiuc009pey.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiCD3D_MOUSE
AccessioniPrimary (citable) accession number: P04235
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: November 22, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot