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Protein

H-2 class I histocompatibility antigen, K-K alpha chain

Gene

H2-K1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the presentation of foreign antigens to the immune system.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity

Enzyme and pathway databases

ReactomeiR-GGA-1236974. ER-Phagosome pathway.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
H-2 class I histocompatibility antigen, K-K alpha chain
Short name:
H-2K(K)
Gene namesi
Name:H2-K1
Synonyms:H2-K
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:95904. H2-K1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 305284ExtracellularSequence analysisAdd
BLAST
Transmembranei306 – 32823HelicalSequence analysisAdd
BLAST
Topological domaini329 – 36941CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, MHC I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 369348H-2 class I histocompatibility antigen, K-K alpha chainPRO_0000018930Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi107 – 1071N-linked (GlcNAc...)1 Publication
Disulfide bondi122 ↔ 185PROSITE-ProRule annotation
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence analysis
Disulfide bondi224 ↔ 280PROSITE-ProRule annotation
Modified residuei354 – 3541PhosphoserineCombined sources
Isoform 2 (identifier: P04223-2)
Modified residuei351 – 3511PhosphoserineCombined sources
Modified residuei354 – 3541PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP04223.
MaxQBiP04223.
PeptideAtlasiP04223.
PRIDEiP04223.

PTM databases

iPTMnetiP04223.

Expressioni

Gene expression databases

CleanExiMM_H2-K1.

Interactioni

Subunit structurei

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).

GO - Molecular functioni

Structurei

Secondary structure

1
369
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi23 – 3311Combined sources
Turni36 – 383Combined sources
Beta strandi42 – 498Combined sources
Beta strandi52 – 598Combined sources
Beta strandi61 – 633Combined sources
Beta strandi67 – 704Combined sources
Helixi71 – 733Combined sources
Helixi78 – 10528Combined sources
Beta strandi110 – 1123Combined sources
Beta strandi115 – 12410Combined sources
Beta strandi128 – 13912Combined sources
Beta strandi142 – 1476Combined sources
Beta strandi154 – 1585Combined sources
Helixi159 – 17012Combined sources
Helixi173 – 18210Combined sources
Helixi184 – 19310Combined sources
Turni196 – 1994Combined sources
Beta strandi207 – 2148Combined sources
Beta strandi216 – 23217Combined sources
Beta strandi235 – 2406Combined sources
Beta strandi249 – 2513Combined sources
Beta strandi258 – 2603Combined sources
Beta strandi262 – 27110Combined sources
Helixi275 – 2773Combined sources
Beta strandi278 – 2836Combined sources
Beta strandi291 – 2933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZT1X-ray2.50A22-297[»]
1ZT7X-ray3.00A/C22-296[»]
ProteinModelPortaliP04223.
SMRiP04223. Positions 22-296.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04223.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 29489Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 11190Alpha-1Add
BLAST
Regioni112 – 20392Alpha-2Add
BLAST
Regioni204 – 29592Alpha-3Add
BLAST
Regioni296 – 30510Connecting peptide

Sequence similaritiesi

Belongs to the MHC class I family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG016709.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04223-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPCMLLLLL AAALAPTQTR AGPHSLRYFH TAVSRPGLGK PRFISVGYVD
60 70 80 90 100
DTQFVRFDSD AENPRYEPRV RWMEQVEPEY WERNTQIAKG NEQIFRVNLR
110 120 130 140 150
TALRYYNQSA GGSHTFQRMY GCEVGSDWRL LRGYEQYAYD GCDYIALNED
160 170 180 190 200
LKTWTAADMA ALITKHKWEQ AGDAERDRAY LEGTCVEWLR RYLQLGNATL
210 220 230 240 250
PRTDSPKAHV TRHSRPEDKV TLRCWALGFY PADITLTWQL NGEELTQDME
260 270 280 290 300
LVETRPAGDG TFQKWASVVV PLGKEQYYTC HVYHQGLPEP LTLRWEPPPS
310 320 330 340 350
TVSNTVIIAV LVVLGAAIVT GAVVAFVMKM RRRNTGGKGG DYALAPGSQT
360
SDLSLPDCKV MVHDPHSLA
Length:369
Mass (Da):41,646
Last modified:March 20, 1987 - v1
Checksum:i9533CF96600A595C
GO
Isoform 2 (identifier: P04223-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-369: VMVHDPHSLA → A

Note: No experimental confirmation available.Combined sources
Show »
Length:360
Mass (Da):40,630
Checksum:i5193B374DBD584CD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501D → E in AAA39654 (PubMed:3840763).Curated
Sequence conflicti98 – 981N → S in AAA53202 (PubMed:3680952).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti173 – 1731D → A.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei360 – 36910VMVHDPHSLA → A in isoform 2. 2 PublicationsVSP_011855

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01652 Genomic DNA. Translation: CAA25816.1.
M18525 Genomic DNA. Translation: AAA53202.1.
U47330 mRNA. Translation: AAB17608.1.
M18964 Genomic DNA. Translation: AAA39568.1.
M11975 mRNA. Translation: AAA39654.1.
M34932 mRNA. Translation: AAA39596.1.
PIRiA02203. HLMSKK.
UniGeneiMm.422886.
Mm.439675.
Mm.440940.
Mm.441651.
Mm.466882.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01652 Genomic DNA. Translation: CAA25816.1.
M18525 Genomic DNA. Translation: AAA53202.1.
U47330 mRNA. Translation: AAB17608.1.
M18964 Genomic DNA. Translation: AAA39568.1.
M11975 mRNA. Translation: AAA39654.1.
M34932 mRNA. Translation: AAA39596.1.
PIRiA02203. HLMSKK.
UniGeneiMm.422886.
Mm.439675.
Mm.440940.
Mm.441651.
Mm.466882.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZT1X-ray2.50A22-297[»]
1ZT7X-ray3.00A/C22-296[»]
ProteinModelPortaliP04223.
SMRiP04223. Positions 22-296.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP04223.

Proteomic databases

EPDiP04223.
MaxQBiP04223.
PeptideAtlasiP04223.
PRIDEiP04223.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:95904. H2-K1.

Phylogenomic databases

HOVERGENiHBG016709.

Enzyme and pathway databases

ReactomeiR-GGA-1236974. ER-Phagosome pathway.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

ChiTaRSiH2-K1. mouse.
EvolutionaryTraceiP04223.
SOURCEiSearch...

Gene expression databases

CleanExiMM_H2-K1.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of the murine H-2Kk gene. Comparison of three H-2K locus alleles."
    Arnold B., Burgert H.-G., Archibald A.L., Kvist S.
    Nucleic Acids Res. 12:9473-9487(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  2. "DNA sequence analysis of the C3H H-2Kk and H-2Dk loci. Evolutionary relationships to H-2 genes from four other mouse strains."
    Watts S., Vogel J.M., Harriman W.D., Itoh T., Stauss H.J., Goodenow R.S.
    J. Immunol. 139:3878-3885(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    Strain: C3H/HeJ.
  3. "Nucleotide sequences of three H-2K and three H-2D complementary DNA clones coding mouse class I MHC heavy chain proteins."
    Wang M., Stepkowski S.M., Hebert J.S., Tian L., Yu J., Kahan B.D.
    Ann. Transplant. 1:26-31(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C3H/HeJ.
    Tissue: Splenocyte.
  4. "The nucleotide sequence of the H-2K gene of C3Hf/HeN mice."
    Minamide L.S., Callahan G.N., Grosveld F.G., Hui K.M.
    Immunogenetics 27:148-152(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-359.
    Strain: C3H/HeN.
  5. "Molecular cloning and sequencing of H-2Kk cDNA: comparison with other H-2 genes and evidence for alternative splicing."
    Reddy E.S., Pan J.Y.
    Gene 38:239-244(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-369 (ISOFORM 2).
  6. "The H-2Kkml mutation: a single nucleotide substitution is responsible for multiple functional differences in a class I MHC molecule."
    Martinko J.M., Solheim J.C., Geliebter J.
    Mol. Immunol. 25:267-274(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 22-313.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-107.
    Tissue: Myoblast.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351 AND SER-354 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiHA1K_MOUSE
AccessioniPrimary (citable) accession number: P04223
Secondary accession number(s): O19459
, Q31165, Q31192, Q95458
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: July 6, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.