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P04222 (1C03_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HLA class I histocompatibility antigen, Cw-3 alpha chain
Alternative name(s):
MHC class I antigen Cw*3
Gene names
Name:HLA-C
Synonyms:HLAC
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the presentation of foreign antigens to the immune system.

Subunit structure

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Post-translational modification

Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.

Polymorphism

The following alleles of Cw-3 are known: Cw*03:02 (Cw3.2), Cw*03:03, Cw*03:04 (Cw3.1), Cw*03:05, Cw*03:06, Cw*03:07, Cw*03:08, Cw*03:09 and Cw*03:13. The sequence shown is that of Cw*03:04.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processHost-virus interaction
Immunity
   Cellular componentMembrane
MHC I
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

innate immune response

Traceable author statement. Source: Reactome

interferon-gamma-mediated signaling pathway

Traceable author statement. Source: Reactome

positive regulation of T cell mediated cytotoxicity

Inferred from electronic annotation. Source: InterPro

regulation of immune response

Traceable author statement. Source: Reactome

type I interferon signaling pathway

Traceable author statement. Source: Reactome

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentER to Golgi transport vesicle membrane

Traceable author statement. Source: Reactome

Golgi membrane

Traceable author statement. Source: Reactome

MHC class I protein complex

Inferred from sequence or structural similarity. Source: UniProt

cell surface

Inferred from direct assay PubMed 9754572. Source: UniProt

early endosome membrane

Traceable author statement. Source: Reactome

integral component of lumenal side of endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionTAP binding

Inferred from direct assay PubMed 8617941. Source: UniProt

peptide antigen binding

Inferred from sequence or structural similarity. Source: UniProt

receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 366342HLA class I histocompatibility antigen, Cw-3 alpha chain
PRO_0000018870

Regions

Topological domain25 – 308284Extracellular Potential
Transmembrane309 – 33325Helical; Potential
Topological domain334 – 36633Cytoplasmic Potential
Domain209 – 29789Ig-like C1-type
Region25 – 11490Alpha-1
Region115 – 20692Alpha-2
Region207 – 29892Alpha-3
Region299 – 30810Connecting peptide

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) By similarity
Disulfide bond125 ↔ 188 Ref.9
Disulfide bond227 ↔ 283 Ref.9

Natural variations

Natural variant731A → E.
Corresponds to variant rs1050409 [ dbSNP | Ensembl ].
VAR_056461
Natural variant761V → M.
Corresponds to variant rs1065382 [ dbSNP | Ensembl ].
VAR_056462
Natural variant901K → N in allele Cw*03:08.
Corresponds to variant rs28626310 [ dbSNP | Ensembl ].
VAR_016569
Natural variant1011S → N in allele Cw*03:07.
VAR_016565
Natural variant1041N → K in allele Cw*03:07.
VAR_016566
Natural variant1151G → R in allele Cw*03:03 and allele Cw*03:13.
VAR_016563
Natural variant1181I → T in allele Cw*03:05 and allele Cw*03:13.
VAR_016564
Natural variant1191I → L in allele Cw*03:02, allele Cw*03:05 and allele Cw*03:13.
VAR_016567
Natural variant1211R → S in allele Cw*03:05.
VAR_016617
Natural variant1271V → L in allele Cw*03:09.
VAR_016618
Natural variant1381D → V in allele Cw*03:06.
VAR_016619
Natural variant1401Y → S in allele Cw*03:02.
VAR_016568
Natural variant2721V → M.
Corresponds to variant rs1050276 [ dbSNP | Ensembl ].
VAR_056463

Experimental info

Sequence conflict331Y → C in CAA25190. Ref.1
Sequence conflict64 – 652AA → DE in CAA25190. Ref.1
Sequence conflict78 – 792QE → RK in CAA25190. Ref.1
Sequence conflict931R → P in CAA25190. Ref.1
Sequence conflict1401Y → H in CAA25190. Ref.1
Sequence conflict1611D → N in CAA25190. Ref.1
Sequence conflict2051R → G in CAA25190. Ref.1

Secondary structure

............................................... 366
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04222 [UniParc].

Last modified August 22, 2003. Version 2.
Checksum: 47E55680EA1F6D97

FASTA36640,861
        10         20         30         40         50         60 
MRVMAPRTLI LLLSGALALT ETWAGSHSMR YFYTAVSRPG RGEPHFIAVG YVDDTQFVRF 

        70         80         90        100        110        120 
DSDAASPRGE PRAPWVEQEG PEYWDRETQK YKRQAQTDRV SLRNLRGYYN QSEAGSHIIQ 

       130        140        150        160        170        180 
RMYGCDVGPD GRLLRGYDQY AYDGKDYIAL NEDLRSWTAA DTAAQITQRK WEAAREAEQL 

       190        200        210        220        230        240 
RAYLEGLCVE WLRRYLKNGK ETLQRAEHPK THVTHHPVSD HEATLRCWAL GFYPAEITLT 

       250        260        270        280        290        300 
WQWDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGEEQR YTCHVQHEGL PEPLTLRWEP 

       310        320        330        340        350        360 
SSQPTIPIVG IVAGLAVLAV LAVLGAVVAV VMCRRKSSGG KGGSCSQAAS SNSAQGSDES 


LIACKA 

« Hide

References

[1]"Complete nucleotide sequence of a gene encoding a functional human class I histocompatibility antigen (HLA-CW3)."
Sodoyer R., Damotte M., Delovitch T.L., Trucy J., Jordan B.R., Strachan T.
EMBO J. 3:879-885(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE CW*03:04).
[2]"The molecular basis for reactivity of anti-Cw1 and anti-Cw3 alloantisera with HLA-B46 haplotypes."
Zemmour J., Gumperz J.E., Hildebrand W.H., Ward F.E., Marsh S.G.E., Williams R.C., Parham P.
Tissue Antigens 39:249-257(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE CW*03:02).
[3]Domena J.D.
Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ALLELES CW*03:03 AND CW*03:04).
[4]"DNA typing of HLA-C locus alleles in Japanese."
Wang H., Tokunaga K., Kuwata S.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ALLELE CW*03:03).
Tissue: Peripheral blood.
[5]"Correction of the HLA-Cw3 genomic sequence tentatively identifies it as HLA-Cw*0304."
Zarling A.L., Smith K.D., Lutz C.T., Lee D.R.
Immunogenetics 44:82-83(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-206 (ALLELE CW*03:04).
[6]"Sequencing based typing for HLA-C. Identification of three new alleles: Cw*0307, Cw*0502 and Cw*0504."
Santos S., Vicario J.L., Garcia-Sanchez F., Aviles M.J., Balas A.
Tissue Antigens 58:34-37(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE CW*03:07).
[7]"Sequence-based typing provides a new look at HLA-C diversity."
Turner S., Ellexson M.E., Hickman H.D., Sidebottom D.A., Fernandez-Vina M., Confer D.L., Hildebrand W.H.
J. Immunol. 161:1406-1413(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELES CW*03:05; CW*03:06; CW*03:08 AND CW*03:09).
[8]"Sequence of two new HLA-Cw alleles: HLA-Cw*0313 and HLA-Cw*1208."
Cox S.T., Ogilvie H., Bohan E.M., Holman R., Prentice H.G., Potter M., Madrigal J.A., Little A.-M.
Tissue Antigens 59:49-51(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE CW*03:13).
[9]"Crystal structure of an NK cell immunoglobulin-like receptor in complex with its class I MHC ligand."
Boyington J.C., Motyka S.A., Schuck P., Brooks A.G., Sun P.D.
Nature 405:537-543(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 25-302 IN COMPLEX WITH KIR2DL2, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00495 Genomic DNA. Translation: CAA25190.1.
M84172 mRNA. Translation: AAA59686.1.
M99389 mRNA. Translation: AAA88088.1.
M99390 mRNA. Translation: AAA88089.1.
D50853 mRNA. Translation: BAA32610.1.
U44064 Genomic DNA. Translation: AAB02773.1.
AF039198 Genomic DNA. Translation: AAD37816.1.
AH006125 Genomic DNA. Translation: AAC17715.1.
AH006126 Genomic DNA. Translation: AAC17716.1.
AH006127 Genomic DNA. Translation: AAC17717.1.
AH006128 Genomic DNA. Translation: AAC17718.1.
AJ298116 Genomic DNA. Translation: CAC18746.1.
PIRHLHUW3. A02190.
I81231.
UniGeneHs.656020.
Hs.743218.
Hs.77961.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EFXX-ray3.00A25-302[»]
ProteinModelPortalP04222.
SMRP04222. Positions 25-302.
ModBaseSearch...
MobiDBSearch...

Polymorphism databases

DMDM34223716.

Proteomic databases

PaxDbP04222.
PRIDEP04222.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000457903; ENSP00000390851; ENSG00000225691.
UCSCuc011hpe.2. human.

Organism-specific databases

GeneCardsGC06M031236.
GC06Mm31313.
HGNCHGNC:4933. HLA-C.
MIM142840. gene.
neXtProtNX_P04222.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42056.
HOVERGENHBG016709.
PhylomeDBP04222.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

CleanExHS_HLA-C.
GenevestigatorP04222.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHLA-C. human.
EvolutionaryTraceP04222.
PROP04222.
SOURCESearch...

Entry information

Entry name1C03_HUMAN
AccessionPrimary (citable) accession number: P04222
Secondary accession number(s): O62883 expand/collapse secondary AC list , O78060, O78068, O78069, P30503, Q29743, Q29992, Q29993, Q9GIK8, Q9TQB4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 22, 2003
Last modified: April 16, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM