P04201 (MAS_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proto-oncogene Mas

Gene names

Name:	MAS1
Synonyms:	MAS

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for angiotensin 1-7 By similarity. Acts specifically as a functional antagonist of AGTR1 (angiotensin-2 type 1 receptor), although it up-regulates AGTR1 receptor levels. Positive regulation of AGTR1 levels occurs through activation of the G-proteins GNA11 and GNAQ, and stimulation of the protein kinase C signaling cascade. The antagonist effect on AGTR1 function is probably due to AGTR1 being physically altered by MAS1. Ref.6 Ref.7 Ref.8
Subunit structure	Interacts with AGTR1 Probable. Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.8.
Involvement in disease	Note=The MAS oncogene has a weak focus-inducing activity in transfected NIH 3T3 cells.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	Was originally (Ref.6) thought to be a receptor for angiotensin II.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Disease	Proto-oncogene
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anatomical structure morphogenesis Traceable author statement. Source: ProtInc cell proliferation Traceable author statement. Source: ProtInc protein kinase C signaling cascade Inferred from mutant phenotype Ref.8. Source: UniProtKB
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	angiotensin type II receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Proto-oncogene Mas		PRO_0000069714
Regions
	Topological domain	1 – 36	36	Extracellular Potential
	Transmembrane	37 – 61	25	Helical; Name=1; Potential
	Topological domain	62 – 65	4	Cytoplasmic Potential
	Transmembrane	66 – 86	21	Helical; Name=2; Potential
	Topological domain	87 – 104	18	Extracellular Potential
	Transmembrane	105 – 128	24	Helical; Name=3; Potential
	Topological domain	129 – 149	21	Cytoplasmic Potential
	Transmembrane	150 – 172	23	Helical; Name=4; Potential
	Topological domain	173 – 185	13	Extracellular Potential
	Transmembrane	186 – 206	21	Helical; Name=5; Potential
	Topological domain	207 – 224	18	Cytoplasmic Potential
	Transmembrane	225 – 245	21	Helical; Name=6; Potential
	Topological domain	246 – 263	18	Extracellular Potential
	Transmembrane	264 – 284	21	Helical; Name=7; Potential
	Topological domain	285 – 325	41	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	16	1	N-linked (GlcNAc...) Potential
	Glycosylation	22	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	138	1	I → D: Fails to activate GNA11. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P04201 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: 3368E7B174744B83	FASTA	325	37,465
	10 20 30 40 50 60 MDGSNVTSFV VEEPTNISTG RNASVGNAHR QIPIVHWVIM SISPVGFVEN GILLWFLCFR 70 80 90 100 110 120 MRRNPFTVYI THLSIADISL LFCIFILSID YALDYELSSG HYYTIVTLSV TFLFGYNTGL 130 140 150 160 170 180 YLLTAISVER CLSVLYPIWY RCHRPKYQSA LVCALLWALS CLVTTMEYVM CIDREEESHS 190 200 210 220 230 240 RNDCRAVIIF IAILSFLVFT PLMLVSSTIL VVKIRKNTWA SHSSKLYIVI MVTIIIFLIF 250 260 270 280 290 300 AMPMRLLYLL YYEYWSTFGN LHHISLLFST INSSANPFIY FFVGSSKKKR FKESLKVVLT 310 320 RAFKDEMQPR RQKDNCNTVT VETVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a new cellular oncogene encoding a protein with multiple potential transmembrane domains." Young D., Waitches G., Birchmeier C., Fasano O., Wigler M. Cell 45:711-719(1986) [PubMed: 3708691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The mas oncogene encodes an angiotensin receptor." Jackson T.R., Blair L.A.C., Marshall J., Goedert M., Hanley M.R. Nature 335:437-440(1988) [PubMed: 3419518] [Abstract] Cited for: PRELIMINARY FUNCTION.
[7]	"G-protein-coupled receptor Mas is a physiological antagonist of the angiotensin II type 1 receptor." Kostenis E., Milligan G., Christopoulos A., Sanchez-Ferrer C.F., Heringer-Walther S., Sexton P.M., Gembardt F., Kellett E., Martini L., Vanderheyden P., Schultheiss H.P., Walther T. Circulation 111:1806-1813(2005) [PubMed: 15809376] [Abstract] Cited for: FUNCTION AS AGTR1 ANTAGONIST, INTERACTION WITH AGTR1.
[8]	"Up-regulation of the angiotensin II type 1 receptor by the MAS proto-oncogene is due to constitutive activation of Gq/G11 by MAS." Canals M., Jenkins L., Kellett E., Milligan G. J. Biol. Chem. 281:16757-16767(2006) [PubMed: 16611642] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ILE-138.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13150 mRNA. Translation: AAA36199.1. CR542261 mRNA. Translation: CAG47057.1. AL035691 Genomic DNA. Translation: CAB72444.1. CH471051 Genomic DNA. Translation: EAW47610.1. CH471051 Genomic DNA. Translation: EAW47611.1. BC069142 mRNA. Translation: AAH69142.1. BC069581 mRNA. Translation: AAH69581.1. BC110454 mRNA. Translation: AAI10455.1.
IPI	IPI00022860.
PIR	TVHUAS. A01375.
RefSeq	NP_002368.1. NM_002377.2.
UniGene	Hs.99900.
3D structure databases
ProteinModelPortal	P04201.
SMR	P04201. Positions 118-148.
ModBase	Search...
Protein-protein interaction databases
STRING	P04201.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P04201.
Polymorphism databases
DMDM	135920.
Proteomic databases
PRIDE	P04201.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252660; ENSP00000252660; ENSG00000130368.
GeneID	4142.
KEGG	hsa:4142.
UCSC	uc003qsz.1. human.
Organism-specific databases
CTD	4142.
GeneCards	GC06P160327.
H-InvDB	HIX0032929.
HGNC	HGNC:6899. MAS1.
HPA	HPA013564.
MIM	165180. gene.
neXtProt	NX_P04201.
PharmGKB	PA30643.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18968.
GeneTree	ENSGT00550000074531.
HOGENOM	HBG403162.
HOVERGEN	HBG098384.
InParanoid	P04201.
OMA	DEMQPRR.
OrthoDB	EOG4SJ5F8.
PhylomeDB	P04201.
Gene expression databases
ArrayExpress	P04201.
Bgee	P04201.
CleanEx	HS_MAS1.
Genevestigator	P04201.
GermOnline	ENSG00000130368. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000820. Mas_TM. [Graphical view]
KO	K04303.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00533. MASONCOGENE.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	16268.
SOURCE	Search...

Entry information

Entry name

MAS_HUMAN

Accession

Primary (citable) accession number: P04201
Secondary accession number(s): E1P5B3, Q2TBC9, Q6FG47

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	January 25, 2012
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.