P04195 (CAHH_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell surface-binding protein

Alternative name(s):
Carbonic anhydrase homolog

Gene names

Ordered Locus Names:	VACWR113
ORF Names:	D8L

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell By similarity.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Virion membrane Potential. Note: Component of the mature virion (MV) membrane Potential.
Induction	Expressed in the late phase of the viral replicative cycle.
Post-translational modification	Apparently non-glycosylated.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Biological process	Host-virus interaction Initiation of viral infection Viral attachment to host cell
Cellular component	Membrane Viral envelope protein Virion
Developmental stage	Late protein
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Cell surface-binding protein		PRO_0000077451
Regions
	Transmembrane	276 – 294	19	Helical; Potential
Amino acid modifications
	Disulfide bond	262		Interchain By similarity
Experimental info
	Sequence conflict	139	1	D → H in AAA48233. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P04195 [UniParc]. Last modified March 20, 1987. Version 1. Checksum: 67F195C8875455BA	FASTA	304	35,446
	10 20 30 40 50 60 MPQQLSPINI ETKKAISNAR LKPLDIHYNE SKPTTIQNTG KLVRINFKGG YISGGFLPNE 70 80 90 100 110 120 YVLSSLHIYW GKEDDYGSNH LIDVYKYSGE INLVHWNKKK YSSYEEAKKH DDGLIIISIF 130 140 150 160 170 180 LQVLDHKNVY FQKIVNQLDS IRSANTSAPF DSVFYLDNLL PSKLDYFTYL GTTINHSADA 190 200 210 220 230 240 VWIIFPTPIN IHSDQLSKFR TLLSSSNHDG KPHYITENYR NPYKLNDDTQ VYYSGEIIRA 250 260 270 280 290 300 ATTSPARENY FMRWLSDLRE TCFSYYQKYI EENKTFAIIA IVFVFILTAI LFFMSRRYSR EKQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment." Niles E.G., Condit R.C., Caro P., Davidson K., Matusick L., Seto J. Virology 153:96-112(1986) [PubMed: 3739227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structural and functional characterization of a cell surface binding protein of vaccinia virus." Maa J.-S., Rodriguez J.F., Esteban M. J. Biol. Chem. 265:1569-1577(1990) [PubMed: 2104847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Vaccinia virus gene D8 encodes a virion transmembrane protein." Niles E.G., Seto J. J. Virol. 62:3772-3778(1988) [PubMed: 3418784] [Abstract] Cited for: IDENTIFICATION OF PROTEIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05190 Genomic DNA. Translation: AAA48233.1. AY243312 Genomic DNA. Translation: AAO89392.1.
PIR	CRVZW. A01146.
RefSeq	YP_232995.1. NC_006998.1.
3D structure databases
HSSP	HSSP built from PDB template 1BCD based on UniProtKB P00918.
ProteinModelPortal	P04195.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3707569.
Phylogenomic databases
ProtClustDB	CLSP2509796.
Family and domain databases
InterPro	IPR001148. a_carbonic_anhydrase. IPR023561. Carbonic_anhydrase_a-class. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
SMART	SM01057. Carb_anhydrase. 1 hit. [Graphical view]
SUPFAM	SSF51069. Euk_COanhd. 1 hit.
PROSITE	PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAHH_VACCW

Accession

Primary (citable) accession number: P04195
Secondary accession number(s): Q76ZR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program