Granzyme B(G,H) precursor - Mus musculus (Mouse)

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P04187 (GRAB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 135. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Granzyme B(G,H)
EC=3.4.21.79

Alternative name(s):
CTLA-1
Cytotoxic cell protease 1
Short name=CCP1
Fragmentin-2

Gene names

Name:	Gzmb
Synonyms:	Ctla-1, Ctla1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis By similarity.
Catalytic activity	Preferential cleavage: -Asp-\|-Xaa- >> -Asn-\|-Xaa- > -Met-\|-Xaa-, -Ser-\|-Xaa-.
Subcellular location	Cytoplasmic granule. Note: Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Apoptosis Cytolysis
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	T cell mediated cytotoxicity Inferred from mutant phenotype PubMed 16618603. Source: MGI cytolysis Inferred from electronic annotation. Source: UniProtKB-KW granzyme-mediated apoptotic signaling pathway Inferred from direct assay PubMed 15454490. Source: MGI induction of apoptosis Inferred from mutant phenotype PubMed 16618603. Source: MGI proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 16618603. Source: MGI
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type peptidase activity Inferred from direct assay PubMed 16618603. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

Propeptide

19 – 20

Activation peptide

PRO_0000027401

Chain

21 – 247

227

Granzyme B(G,H)

PRO_0000027402

Regions

Domain

21 – 245

225

Peptidase S1

Sites

Active site

Charge relay system By similarity

Active site

108

Charge relay system By similarity

Active site

203

Charge relay system By similarity

Site

228

Mediates preference for Asp-containing substrates By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

182

N-linked (GlcNAc...) Potential

Disulfide bond

49 ↔ 65

By similarity

Disulfide bond

142 ↔ 209

By similarity

Disulfide bond

173 ↔ 188

By similarity

Secondary structure

247

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P04187 [UniParc].

Last modified March 20, 1987. Version 1.
Checksum: 996BCD199965C6D6
Show »

FASTA

247

27,470

        10         20         30         40         50         60 
MKILLLLLTL SLASRTKAGE IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL 

        70         80         90        100        110        120 
TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR 

       130        140        150        160        170        180 
TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR 

       190        200        210        220        230        240 
YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI 


KKTMKSS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes." Lobe C.G., Finlay B.B., Paranchych W., Paetkau V.H., Bleackley R.C. Science 232:858-861(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Organization of two genes encoding cytotoxic T lymphocyte-specific serine proteases CCPI and CCPII." Lobe C.G., Upton C., Duggan B., Ehrman N., Letellier M., Bell J., McFadden G., Bleackley R.C. Biochemistry 27:6941-6946(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14." Brunet J.-F., Dosseto M., Denizot F., Mattei M.-G., Clark W.R., Haqqi T.M., Ferrier P., Nabholz M., Schmitt-Verhulst A.-M., Luciani M.-F., Golstein P. Nature 322:268-271(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Genetic mapping of 40 cDNA clones on the mouse genome by PCR." Ko M.S., Wang X., Horton J.H., Hagen M.D., Takahashi N., Maezaki Y., Nadeau J.H. Mamm. Genome 5:349-355(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 227-247. Strain: C57BL/6J.
[6]	"A family of serine esterases in lytic granules of cytolytic T lymphocytes." Masson D., Tschopp J. Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-40.
[7]	"Comparative molecular model building of two serine proteinases from cytotoxic T lymphocytes." Murphy M.E.P., Moult J., Bleackley R.C., Gershenfeld H., Weissman I.L., James M.N.G. Proteins 4:190-204(1988) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04072 mRNA. Translation: CAA27715.1.
M12302 mRNA. Translation: AAA37383.1.
M22526 Genomic DNA. Translation: AAB61756.1.
BC002085 mRNA. Translation: AAH02085.1.
U05707 Genomic DNA. Translation: AAB60470.1.

IPI

IPI00114066.

PIR

PRMSCL. A94288.

RefSeq

NP_038570.1. NM_013542.2.

UniGene

Mm.14874.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CP1	model	-	A	21-247	[»]

ProteinModelPortal

P04187.

SMR

P04187. Positions 21-245.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-562N.

MINT

MINT-4096650.

Protein family/group databases

MEROPS

S01.136.

PTM databases

PhosphoSite

P04187.

Proteomic databases

PaxDb

P04187.

PRIDE

P04187.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000015581; ENSMUSP00000015581; ENSMUSG00000015437.

GeneID

14939.

KEGG

mmu:14939.

UCSC

uc007ubv.1. mouse.

Organism-specific databases

CTD

3002.

MGI

MGI:109267. Gzmb.

Phylogenomic databases

eggNOG

COG5640.

HOGENOM

HOG000251820.

HOVERGEN

HBG013304.

InParanoid

P04187.

K01353.

OMA

DSECEFL.

OrthoDB

EOG4KWJTW.

Gene expression databases

ArrayExpress

P04187.

Bgee

P04187.

CleanEx

MM_GZMB.

Genevestigator

P04187.

GermOnline

ENSMUSG00000015437. Mus musculus.

Family and domain databases

InterPro

IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

287263.

SOURCE

Search...

Entry information

Entry name

GRAB_MOUSE

Accession

Primary (citable) accession number: P04187

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	March 20, 1987
Last modified:	May 29, 2013
This is version 135 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents