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P04187

- GRAB_MOUSE

UniProt

P04187 - GRAB_MOUSE

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Protein
Granzyme B(G,H)
Gene
Gzmb, Ctla-1, Ctla1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis By similarity.

Catalytic activityi

Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Charge relay system By similarity
Active sitei108 – 1081Charge relay system By similarity
Active sitei203 – 2031Charge relay system By similarity
Sitei228 – 2281Mediates preference for Asp-containing substrates By similarity

GO - Molecular functioni

  1. protein binding Source: MGI
  2. serine-type endopeptidase activity Source: RefGenome
  3. serine-type peptidase activity Source: MGI

GO - Biological processi

  1. T cell mediated cytotoxicity Source: MGI
  2. cytolysis Source: UniProtKB-KW
  3. granzyme-mediated apoptotic signaling pathway Source: MGI
  4. proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Apoptosis, Cytolysis

Enzyme and pathway databases

ReactomeiREACT_196593. NOTCH2 intracellular domain regulates transcription.
REACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

Protein family/group databases

MEROPSiS01.136.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme B(G,H) (EC:3.4.21.79)
Alternative name(s):
CTLA-1
Cytotoxic cell protease 1
Short name:
CCP1
Fragmentin-2
Gene namesi
Name:Gzmb
Synonyms:Ctla-1, Ctla1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:109267. Gzmb.

Subcellular locationi

Cytoplasmic granule
Note: Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

GO - Cellular componenti

  1. cytolytic granule Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: Reactome
  4. secretory granule Source: RefGenome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818
Add
BLAST
Propeptidei19 – 202Activation peptide
PRO_0000027401
Chaini21 – 247227Granzyme B(G,H)
PRO_0000027402Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 65 By similarity
Glycosylationi71 – 711N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi142 ↔ 209 By similarity
Disulfide bondi173 ↔ 188 By similarity
Glycosylationi182 – 1821N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP04187.
PaxDbiP04187.
PRIDEiP04187.

PTM databases

PhosphoSiteiP04187.

Expressioni

Gene expression databases

ArrayExpressiP04187.
BgeeiP04187.
CleanExiMM_GZMB.
GenevestigatoriP04187.

Interactioni

Protein-protein interaction databases

BioGridi200135. 5 interactions.
DIPiDIP-562N.
IntActiP04187. 1 interaction.
MINTiMINT-4096650.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi35 – 417
Beta strandi43 – 5513
Beta strandi58 – 614
Helixi63 – 653
Beta strandi68 – 758
Beta strandi87 – 9610
Turni102 – 1043
Beta strandi110 – 1167
Beta strandi141 – 1477
Beta strandi149 – 1513
Beta strandi154 – 1563
Beta strandi161 – 1688
Helixi170 – 1745
Turni175 – 1773
Helixi178 – 1803
Helixi183 – 1853
Beta strandi186 – 1894
Turni200 – 2045
Beta strandi206 – 2094
Beta strandi212 – 2198
Beta strandi228 – 2325
Helixi233 – 24513

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CP1model-A21-247[»]
ProteinModelPortaliP04187.
SMRiP04187. Positions 21-245.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 245225Peptidase S1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP04187.
KOiK01353.
OMAiEECESKL.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP04187.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04187-1 [UniParc]FASTAAdd to Basket

« Hide

MKILLLLLTL SLASRTKAGE IIGGHEVKPH SRPYMALLSI KDQQPEAICG    50
GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY 100
NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM 150
APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR 200
GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS 247
Length:247
Mass (Da):27,470
Last modified:March 20, 1987 - v1
Checksum:i996BCD199965C6D6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04072 mRNA. Translation: CAA27715.1.
M12302 mRNA. Translation: AAA37383.1.
M22526 Genomic DNA. Translation: AAB61756.1.
BC002085 mRNA. Translation: AAH02085.1.
U05707 Genomic DNA. Translation: AAB60470.1.
CCDSiCCDS27147.1.
PIRiA94288. PRMSCL.
RefSeqiNP_038570.1. NM_013542.2.
UniGeneiMm.14874.

Genome annotation databases

EnsembliENSMUST00000015581; ENSMUSP00000015581; ENSMUSG00000015437.
GeneIDi14939.
KEGGimmu:14939.
UCSCiuc007ubv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04072 mRNA. Translation: CAA27715.1 .
M12302 mRNA. Translation: AAA37383.1 .
M22526 Genomic DNA. Translation: AAB61756.1 .
BC002085 mRNA. Translation: AAH02085.1 .
U05707 Genomic DNA. Translation: AAB60470.1 .
CCDSi CCDS27147.1.
PIRi A94288. PRMSCL.
RefSeqi NP_038570.1. NM_013542.2.
UniGenei Mm.14874.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CP1 model - A 21-247 [» ]
ProteinModelPortali P04187.
SMRi P04187. Positions 21-245.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200135. 5 interactions.
DIPi DIP-562N.
IntActi P04187. 1 interaction.
MINTi MINT-4096650.

Protein family/group databases

MEROPSi S01.136.

PTM databases

PhosphoSitei P04187.

Proteomic databases

MaxQBi P04187.
PaxDbi P04187.
PRIDEi P04187.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000015581 ; ENSMUSP00000015581 ; ENSMUSG00000015437 .
GeneIDi 14939.
KEGGi mmu:14939.
UCSCi uc007ubv.1. mouse.

Organism-specific databases

CTDi 3002.
MGIi MGI:109267. Gzmb.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi P04187.
KOi K01353.
OMAi EECESKL.
OrthoDBi EOG7RRF7Z.
PhylomeDBi P04187.
TreeFami TF333630.

Enzyme and pathway databases

Reactomei REACT_196593. NOTCH2 intracellular domain regulates transcription.
REACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

Miscellaneous databases

NextBioi 287263.
PROi P04187.
SOURCEi Search...

Gene expression databases

ArrayExpressi P04187.
Bgeei P04187.
CleanExi MM_GZMB.
Genevestigatori P04187.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes."
    Lobe C.G., Finlay B.B., Paranchych W., Paetkau V.H., Bleackley R.C.
    Science 232:858-861(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of two genes encoding cytotoxic T lymphocyte-specific serine proteases CCPI and CCPII."
    Lobe C.G., Upton C., Duggan B., Ehrman N., Letellier M., Bell J., McFadden G., Bleackley R.C.
    Biochemistry 27:6941-6946(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14."
    Brunet J.-F., Dosseto M., Denizot F., Mattei M.-G., Clark W.R., Haqqi T.M., Ferrier P., Nabholz M., Schmitt-Verhulst A.-M., Luciani M.-F., Golstein P.
    Nature 322:268-271(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Genetic mapping of 40 cDNA clones on the mouse genome by PCR."
    Ko M.S., Wang X., Horton J.H., Hagen M.D., Takahashi N., Maezaki Y., Nadeau J.H.
    Mamm. Genome 5:349-355(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 227-247.
    Strain: C57BL/6J.
  6. "A family of serine esterases in lytic granules of cytolytic T lymphocytes."
    Masson D., Tschopp J.
    Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-40.
  7. "Comparative molecular model building of two serine proteinases from cytotoxic T lymphocytes."
    Murphy M.E.P., Moult J., Bleackley R.C., Gershenfeld H., Weissman I.L., James M.N.G.
    Proteins 4:190-204(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiGRAB_MOUSE
AccessioniPrimary (citable) accession number: P04187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: September 3, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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