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P04187

- GRAB_MOUSE

UniProt

P04187 - GRAB_MOUSE

Protein

Granzyme B(G,H)

Gene

Gzmb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 1 (20 Mar 1987)
      Previous versions | rss
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    Functioni

    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis By similarity.By similarity

    Catalytic activityi

    Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei64 – 641Charge relay systemBy similarity
    Active sitei108 – 1081Charge relay systemBy similarity
    Active sitei203 – 2031Charge relay systemBy similarity
    Sitei228 – 2281Mediates preference for Asp-containing substratesBy similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. serine-type endopeptidase activity Source: RefGenome
    3. serine-type peptidase activity Source: MGI

    GO - Biological processi

    1. cytolysis Source: UniProtKB-KW
    2. granzyme-mediated apoptotic signaling pathway Source: MGI
    3. proteolysis Source: RefGenome
    4. T cell mediated cytotoxicity Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Apoptosis, Cytolysis

    Enzyme and pathway databases

    ReactomeiREACT_196593. NOTCH2 intracellular domain regulates transcription.
    REACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

    Protein family/group databases

    MEROPSiS01.136.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granzyme B(G,H) (EC:3.4.21.79)
    Alternative name(s):
    CTLA-1
    Cytotoxic cell protease 1
    Short name:
    CCP1
    Fragmentin-2
    Gene namesi
    Name:Gzmb
    Synonyms:Ctla-1, Ctla1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:109267. Gzmb.

    Subcellular locationi

    Cytoplasmic granule
    Note: Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

    GO - Cellular componenti

    1. cytolytic granule Source: MGI
    2. cytoplasm Source: MGI
    3. cytosol Source: Reactome
    4. secretory granule Source: RefGenome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Propeptidei19 – 202Activation peptide1 PublicationPRO_0000027401
    Chaini21 – 247227Granzyme B(G,H)PRO_0000027402Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi142 ↔ 209PROSITE-ProRule annotation
    Disulfide bondi173 ↔ 188PROSITE-ProRule annotation
    Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP04187.
    PaxDbiP04187.
    PRIDEiP04187.

    PTM databases

    PhosphoSiteiP04187.

    Expressioni

    Gene expression databases

    ArrayExpressiP04187.
    BgeeiP04187.
    CleanExiMM_GZMB.
    GenevestigatoriP04187.

    Interactioni

    Protein-protein interaction databases

    BioGridi200135. 5 interactions.
    DIPiDIP-562N.
    IntActiP04187. 1 interaction.
    MINTiMINT-4096650.

    Structurei

    Secondary structure

    1
    247
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi35 – 417
    Beta strandi43 – 5513
    Beta strandi58 – 614
    Helixi63 – 653
    Beta strandi68 – 758
    Beta strandi87 – 9610
    Turni102 – 1043
    Beta strandi110 – 1167
    Beta strandi141 – 1477
    Beta strandi149 – 1513
    Beta strandi154 – 1563
    Beta strandi161 – 1688
    Helixi170 – 1745
    Turni175 – 1773
    Helixi178 – 1803
    Helixi183 – 1853
    Beta strandi186 – 1894
    Turni200 – 2045
    Beta strandi206 – 2094
    Beta strandi212 – 2198
    Beta strandi228 – 2325
    Helixi233 – 24513

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CP1model-A21-247[»]
    ProteinModelPortaliP04187.
    SMRiP04187. Positions 21-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 245225Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP04187.
    KOiK01353.
    OMAiEECESKL.
    OrthoDBiEOG7RRF7Z.
    PhylomeDBiP04187.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04187-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKILLLLLTL SLASRTKAGE IIGGHEVKPH SRPYMALLSI KDQQPEAICG    50
    GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY 100
    NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM 150
    APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR 200
    GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS 247
    Length:247
    Mass (Da):27,470
    Last modified:March 20, 1987 - v1
    Checksum:i996BCD199965C6D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04072 mRNA. Translation: CAA27715.1.
    M12302 mRNA. Translation: AAA37383.1.
    M22526 Genomic DNA. Translation: AAB61756.1.
    BC002085 mRNA. Translation: AAH02085.1.
    U05707 Genomic DNA. Translation: AAB60470.1.
    CCDSiCCDS27147.1.
    PIRiA94288. PRMSCL.
    RefSeqiNP_038570.1. NM_013542.2.
    UniGeneiMm.14874.

    Genome annotation databases

    EnsembliENSMUST00000015581; ENSMUSP00000015581; ENSMUSG00000015437.
    GeneIDi14939.
    KEGGimmu:14939.
    UCSCiuc007ubv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04072 mRNA. Translation: CAA27715.1 .
    M12302 mRNA. Translation: AAA37383.1 .
    M22526 Genomic DNA. Translation: AAB61756.1 .
    BC002085 mRNA. Translation: AAH02085.1 .
    U05707 Genomic DNA. Translation: AAB60470.1 .
    CCDSi CCDS27147.1.
    PIRi A94288. PRMSCL.
    RefSeqi NP_038570.1. NM_013542.2.
    UniGenei Mm.14874.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CP1 model - A 21-247 [» ]
    ProteinModelPortali P04187.
    SMRi P04187. Positions 21-245.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200135. 5 interactions.
    DIPi DIP-562N.
    IntActi P04187. 1 interaction.
    MINTi MINT-4096650.

    Protein family/group databases

    MEROPSi S01.136.

    PTM databases

    PhosphoSitei P04187.

    Proteomic databases

    MaxQBi P04187.
    PaxDbi P04187.
    PRIDEi P04187.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015581 ; ENSMUSP00000015581 ; ENSMUSG00000015437 .
    GeneIDi 14939.
    KEGGi mmu:14939.
    UCSCi uc007ubv.1. mouse.

    Organism-specific databases

    CTDi 3002.
    MGIi MGI:109267. Gzmb.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P04187.
    KOi K01353.
    OMAi EECESKL.
    OrthoDBi EOG7RRF7Z.
    PhylomeDBi P04187.
    TreeFami TF333630.

    Enzyme and pathway databases

    Reactomei REACT_196593. NOTCH2 intracellular domain regulates transcription.
    REACT_208000. Activation, myristolyation of BID and translocation to mitochondria.

    Miscellaneous databases

    NextBioi 287263.
    PROi P04187.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04187.
    Bgeei P04187.
    CleanExi MM_GZMB.
    Genevestigatori P04187.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes."
      Lobe C.G., Finlay B.B., Paranchych W., Paetkau V.H., Bleackley R.C.
      Science 232:858-861(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organization of two genes encoding cytotoxic T lymphocyte-specific serine proteases CCPI and CCPII."
      Lobe C.G., Upton C., Duggan B., Ehrman N., Letellier M., Bell J., McFadden G., Bleackley R.C.
      Biochemistry 27:6941-6946(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14."
      Brunet J.-F., Dosseto M., Denizot F., Mattei M.-G., Clark W.R., Haqqi T.M., Ferrier P., Nabholz M., Schmitt-Verhulst A.-M., Luciani M.-F., Golstein P.
      Nature 322:268-271(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Genetic mapping of 40 cDNA clones on the mouse genome by PCR."
      Ko M.S., Wang X., Horton J.H., Hagen M.D., Takahashi N., Maezaki Y., Nadeau J.H.
      Mamm. Genome 5:349-355(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 227-247.
      Strain: C57BL/6J.
    6. "A family of serine esterases in lytic granules of cytolytic T lymphocytes."
      Masson D., Tschopp J.
      Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-40.
    7. "Comparative molecular model building of two serine proteinases from cytotoxic T lymphocytes."
      Murphy M.E.P., Moult J., Bleackley R.C., Gershenfeld H., Weissman I.L., James M.N.G.
      Proteins 4:190-204(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiGRAB_MOUSE
    AccessioniPrimary (citable) accession number: P04187
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: March 20, 1987
    Last modified: October 1, 2014
    This is version 148 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3