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Protein

Granzyme B(G,H)

Gene

Gzmb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (By similarity).By similarity

Catalytic activityi

Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Charge relay systemBy similarity
Active sitei108 – 1081Charge relay systemBy similarity
Active sitei203 – 2031Charge relay systemBy similarity
Sitei228 – 2281Mediates preference for Asp-containing substratesBy similarity

GO - Molecular functioni

  • serine-type endopeptidase activity Source: GO_Central
  • serine-type peptidase activity Source: MGI

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • granzyme-mediated apoptotic signaling pathway Source: MGI
  • protein processing Source: GO_Central
  • T cell mediated cytotoxicity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Apoptosis, Cytolysis

Enzyme and pathway databases

ReactomeiREACT_278898. NOTCH2 intracellular domain regulates transcription.
REACT_335353. Activation, myristolyation of BID and translocation to mitochondria.

Protein family/group databases

MEROPSiS01.136.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme B(G,H) (EC:3.4.21.79)
Alternative name(s):
CTLA-1
Cytotoxic cell protease 1
Short name:
CCP1
Fragmentin-2
Gene namesi
Name:Gzmb
Synonyms:Ctla-1, Ctla1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:109267. Gzmb.

Subcellular locationi

  • Cytoplasmic granule

  • Note: Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.

GO - Cellular componenti

  • cytolytic granule Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Propeptidei19 – 202Activation peptide1 PublicationPRO_0000027401
Chaini21 – 247227Granzyme B(G,H)PRO_0000027402Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi142 ↔ 209PROSITE-ProRule annotation
Disulfide bondi173 ↔ 188PROSITE-ProRule annotation
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP04187.
PaxDbiP04187.
PRIDEiP04187.

PTM databases

PhosphoSiteiP04187.

Expressioni

Gene expression databases

BgeeiP04187.
CleanExiMM_GZMB.
ExpressionAtlasiP04187. baseline and differential.
GenevisibleiP04187. MM.

Interactioni

Protein-protein interaction databases

BioGridi200135. 5 interactions.
DIPiDIP-562N.
IntActiP04187. 1 interaction.
MINTiMINT-4096650.
STRINGi10090.ENSMUSP00000015581.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CP1model-A21-247[»]
ProteinModelPortaliP04187.
SMRiP04187. Positions 21-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 245225Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP04187.
KOiK01353.
OMAiEECESKL.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP04187.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILLLLLTL SLASRTKAGE IIGGHEVKPH SRPYMALLSI KDQQPEAICG
60 70 80 90 100
GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY
110 120 130 140 150
NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM
160 170 180 190 200
APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR
210 220 230 240
GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS
Length:247
Mass (Da):27,470
Last modified:March 20, 1987 - v1
Checksum:i996BCD199965C6D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04072 mRNA. Translation: CAA27715.1.
M12302 mRNA. Translation: AAA37383.1.
M22526 Genomic DNA. Translation: AAB61756.1.
BC002085 mRNA. Translation: AAH02085.1.
U05707 Genomic DNA. Translation: AAB60470.1.
CCDSiCCDS27147.1.
PIRiA94288. PRMSCL.
RefSeqiNP_038570.1. NM_013542.2.
UniGeneiMm.14874.

Genome annotation databases

EnsembliENSMUST00000015581; ENSMUSP00000015581; ENSMUSG00000015437.
GeneIDi14939.
KEGGimmu:14939.
UCSCiuc007ubv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04072 mRNA. Translation: CAA27715.1.
M12302 mRNA. Translation: AAA37383.1.
M22526 Genomic DNA. Translation: AAB61756.1.
BC002085 mRNA. Translation: AAH02085.1.
U05707 Genomic DNA. Translation: AAB60470.1.
CCDSiCCDS27147.1.
PIRiA94288. PRMSCL.
RefSeqiNP_038570.1. NM_013542.2.
UniGeneiMm.14874.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CP1model-A21-247[»]
ProteinModelPortaliP04187.
SMRiP04187. Positions 21-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200135. 5 interactions.
DIPiDIP-562N.
IntActiP04187. 1 interaction.
MINTiMINT-4096650.
STRINGi10090.ENSMUSP00000015581.

Protein family/group databases

MEROPSiS01.136.

PTM databases

PhosphoSiteiP04187.

Proteomic databases

MaxQBiP04187.
PaxDbiP04187.
PRIDEiP04187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015581; ENSMUSP00000015581; ENSMUSG00000015437.
GeneIDi14939.
KEGGimmu:14939.
UCSCiuc007ubv.1. mouse.

Organism-specific databases

CTDi3002.
MGIiMGI:109267. Gzmb.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP04187.
KOiK01353.
OMAiEECESKL.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP04187.
TreeFamiTF333630.

Enzyme and pathway databases

ReactomeiREACT_278898. NOTCH2 intracellular domain regulates transcription.
REACT_335353. Activation, myristolyation of BID and translocation to mitochondria.

Miscellaneous databases

NextBioi287263.
PROiP04187.
SOURCEiSearch...

Gene expression databases

BgeeiP04187.
CleanExiMM_GZMB.
ExpressionAtlasiP04187. baseline and differential.
GenevisibleiP04187. MM.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel serine proteases encoded by two cytotoxic T lymphocyte-specific genes."
    Lobe C.G., Finlay B.B., Paranchych W., Paetkau V.H., Bleackley R.C.
    Science 232:858-861(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organization of two genes encoding cytotoxic T lymphocyte-specific serine proteases CCPI and CCPII."
    Lobe C.G., Upton C., Duggan B., Ehrman N., Letellier M., Bell J., McFadden G., Bleackley R.C.
    Biochemistry 27:6941-6946(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The inducible cytotoxic T-lymphocyte-associated gene transcript CTLA-1 sequence and gene localization to mouse chromosome 14."
    Brunet J.-F., Dosseto M., Denizot F., Mattei M.-G., Clark W.R., Haqqi T.M., Ferrier P., Nabholz M., Schmitt-Verhulst A.-M., Luciani M.-F., Golstein P.
    Nature 322:268-271(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Genetic mapping of 40 cDNA clones on the mouse genome by PCR."
    Ko M.S., Wang X., Horton J.H., Hagen M.D., Takahashi N., Maezaki Y., Nadeau J.H.
    Mamm. Genome 5:349-355(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 227-247.
    Strain: C57BL/6J.
  6. "A family of serine esterases in lytic granules of cytolytic T lymphocytes."
    Masson D., Tschopp J.
    Cell 49:679-685(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-40.
  7. "Comparative molecular model building of two serine proteinases from cytotoxic T lymphocytes."
    Murphy M.E.P., Moult J., Bleackley R.C., Gershenfeld H., Weissman I.L., James M.N.G.
    Proteins 4:190-204(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiGRAB_MOUSE
AccessioniPrimary (citable) accession number: P04187
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: July 22, 2015
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.