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P04186

- CFAB_MOUSE

UniProt

P04186 - CFAB_MOUSE

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Protein

Complement factor B

Gene

Cfb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.

Catalytic activityi

Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei523 – 5231Charge relay system
Active sitei573 – 5731Charge relay system
Active sitei696 – 6961Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. cell proliferation Source: MGI
  2. complement activation, alternative pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement alternate pathway, Immunity, Innate immunity

Enzyme and pathway databases

BRENDAi3.4.21.47. 3474.
ReactomeiREACT_198562. Regulation of Complement cascade.
REACT_213087. Activation of C3 and C5.
REACT_218037. Alternative complement activation.

Protein family/group databases

MEROPSiS01.196.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor B (EC:3.4.21.47)
Alternative name(s):
C3/C5 convertase
Cleaved into the following 2 chains:
Gene namesi
Name:Cfb
Synonyms:Bf, H2-Bf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:105975. Cfb.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 761739Complement factor BPRO_0000027548Add
BLAST
Chaini23 – 256234Complement factor B Ba fragmentPRO_0000027549Add
BLAST
Chaini257 – 761505Complement factor B Bb fragmentPRO_0000027550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 73By similarity
Disulfide bondi59 ↔ 95By similarity
Disulfide bondi100 ↔ 142By similarity
Glycosylationi119 – 1191N-linked (GlcNAc...)1 Publication
Disulfide bondi128 ↔ 155By similarity
Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi162 ↔ 202By similarity
Disulfide bondi188 ↔ 215By similarity
Glycosylationi282 – 2821N-linked (GlcNAc...)1 Publication
Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi508 ↔ 524By similarity
Disulfide bondi692 ↔ 722By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP04186.
PaxDbiP04186.
PRIDEiP04186.

PTM databases

PhosphoSiteiP04186.

Expressioni

Gene expression databases

BgeeiP04186.
CleanExiMM_CFB.
ExpressionAtlasiP04186. baseline.
GenevestigatoriP04186.

Interactioni

Protein-protein interaction databases

IntActiP04186. 1 interaction.
MINTiMINT-1865229.
STRINGi10090.ENSMUSP00000117677.

Structurei

3D structure databases

ProteinModelPortaliP04186.
SMRiP04186. Positions 33-761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 9766Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini98 – 15760Sushi 2PROSITE-ProRule annotationAdd
BLAST
Domaini160 – 21758Sushi 3PROSITE-ProRule annotationAdd
BLAST
Domaini267 – 466200VWFAPROSITE-ProRule annotationAdd
BLAST
Domaini474 – 754281Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Domaini

The unliganded VWA domain has an inactive 'locked' conformation whereby the scissile Arg-256|Lys-257 bond is protected from proteolytic activation.By similarity

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 3 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiNOG306397.
HOGENOMiHOG000038034.
HOVERGENiHBG002567.
InParanoidiP04186.
OrthoDBiEOG71CFK5.
PhylomeDBiP04186.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR011360. Compl_C2_B.
IPR028341. Complement_B.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00084. Sushi. 3 hits.
PF00089. Trypsin. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PIRSFiPIRSF001154. Compl_C2_B. 1 hit.
PIRSF500181. Complement_B. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 3 hits.
SM00020. Tryp_SPc. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF57535. SSF57535. 3 hits.
PROSITEiPS50923. SUSHI. 3 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04186-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESPQLCLVL LVLGFSSGGV SATPVLEARP QVSCSLEGVE IKGGSFQLLQ
60 70 80 90 100
GGQALEYLCP SGFYPYPVQT RTCRSTGSWS DLQTRDQKIV QKAECRAIRC
110 120 130 140 150
PRPQDFENGE FWPRSPFYNL SDQISFQCYD GYVLRGSANR TCQENGRWDG
160 170 180 190 200
QTAICDDGAG YCPNPGIPIG TRKVGSQYRL EDIVTYHCSR GLVLRGSQKR
210 220 230 240 250
KCQEGGSWSG TEPSCQDSFM YDSPQEVAEA FLSSLTETIE GADAEDGHSP
260 270 280 290 300
GEQQKRKIVL DPSGSMNIYL VLDGSDSIGS SNFTGAKRCL TNLIEKVASY
310 320 330 340 350
GVRPRYGLLT YATVPKVLVR VSDERSSDAD WVTEKLNQIS YEDHKLKSGT
360 370 380 390 400
NTKRALQAVY SMMSWAGDAP PEGWNRTRHV IIIMTDGLHN MGGNPVTVIQ
410 420 430 440 450
DIRALLDIGR DPKNPREDYL DVYVFGVGPL VDSVNINALA SKKDNEHHVF
460 470 480 490 500
KVKDMEDLEN VFYQMIDETK SLSLCGMVWE HKKGNDYHKQ PWQAKISVTR
510 520 530 540 550
PLKGHETCMG AVVSEYFVLT AAHCFMVDDQ KHSIKVSVGG QRRDLEIEEV
560 570 580 590 600
LFHPKYNING KKAEGIPEFY DYDVALVKLK NKLKYGQTLR PICLPCTEGT
610 620 630 640 650
TRALRLPQTA TCKQHKEQLL PVKDVKALFV SEQGKSLTRK EVYIKNGDKK
660 670 680 690 700
ASCERDATKA QGYEKVKDAS EVVTPRFLCT GGVDPYADPN TCKGDSGGPL
710 720 730 740 750
IVHKRSRFIQ VGVISWGVVD VCRDQRRQQL VPSYARDFHI NLFQVLPWLK
760
DKLKDEDLGF L
Length:761
Mass (Da):85,004
Last modified:August 1, 1991 - v2
Checksum:i9E9015448A61020E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti730 – 7301L → P in AAA39549. (PubMed:6689022)Curated
Sequence conflicti745 – 7451V → E in AAA39549. (PubMed:6689022)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60646
, M60629, M60630, M60631, M60632, M60633, M60634, M60635, M60636, M60637, M60638, M60639, M60640, M60641, M60642, M60643, M60644, M60645 Genomic DNA. Translation: AAA37379.1.
AF049850 Genomic DNA. Translation: AAC05283.1.
AF109906 Genomic DNA. Translation: AAC84160.1.
BC005451 mRNA. Translation: AAH05451.1.
K01496 mRNA. Translation: AAA39549.1.
K01497 mRNA. Translation: AAA39550.1.
K01498 mRNA. Translation: AAA39551.1.
M57890 mRNA. Translation: AAA63293.1.
PIRiA38875. BBMS.
UniGeneiMm.653.

Genome annotation databases

EnsembliENSMUST00000128767; ENSMUSP00000119977; ENSMUSG00000090231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60646
, M60629 , M60630 , M60631 , M60632 , M60633 , M60634 , M60635 , M60636 , M60637 , M60638 , M60639 , M60640 , M60641 , M60642 , M60643 , M60644 , M60645 Genomic DNA. Translation: AAA37379.1 .
AF049850 Genomic DNA. Translation: AAC05283.1 .
AF109906 Genomic DNA. Translation: AAC84160.1 .
BC005451 mRNA. Translation: AAH05451.1 .
K01496 mRNA. Translation: AAA39549.1 .
K01497 mRNA. Translation: AAA39550.1 .
K01498 mRNA. Translation: AAA39551.1 .
M57890 mRNA. Translation: AAA63293.1 .
PIRi A38875. BBMS.
UniGenei Mm.653.

3D structure databases

ProteinModelPortali P04186.
SMRi P04186. Positions 33-761.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P04186. 1 interaction.
MINTi MINT-1865229.
STRINGi 10090.ENSMUSP00000117677.

Protein family/group databases

MEROPSi S01.196.

PTM databases

PhosphoSitei P04186.

Proteomic databases

MaxQBi P04186.
PaxDbi P04186.
PRIDEi P04186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000128767 ; ENSMUSP00000119977 ; ENSMUSG00000090231 .

Organism-specific databases

MGIi MGI:105975. Cfb.

Phylogenomic databases

eggNOGi NOG306397.
HOGENOMi HOG000038034.
HOVERGENi HBG002567.
InParanoidi P04186.
OrthoDBi EOG71CFK5.
PhylomeDBi P04186.

Enzyme and pathway databases

BRENDAi 3.4.21.47. 3474.
Reactomei REACT_198562. Regulation of Complement cascade.
REACT_213087. Activation of C3 and C5.
REACT_218037. Alternative complement activation.

Miscellaneous databases

PROi P04186.
SOURCEi Search...

Gene expression databases

Bgeei P04186.
CleanExi MM_CFB.
ExpressionAtlasi P04186. baseline.
Genevestigatori P04186.

Family and domain databases

Gene3Di 3.40.50.410. 1 hit.
InterProi IPR011360. Compl_C2_B.
IPR028341. Complement_B.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF00084. Sushi. 3 hits.
PF00089. Trypsin. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view ]
PIRSFi PIRSF001154. Compl_C2_B. 1 hit.
PIRSF500181. Complement_B. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00032. CCP. 3 hits.
SM00020. Tryp_SPc. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF57535. SSF57535. 3 hits.
PROSITEi PS50923. SUSHI. 3 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine complement C2 and factor B genomic and cDNA cloning reveals different mechanisms for multiple transcripts of C2 and B."
    Ishikawa N., Nonaka M., Wetsel R.A., Colten H.R.
    J. Biol. Chem. 265:19040-19046(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "Phylogenetic conservation of a class III major histocompatibility complex antigen, factor B. Isolation and nucleotide sequencing of mouse factor B cDNA clones."
    Sackstein R., Colten H.R., Woods D.E.
    J. Biol. Chem. 258:14693-14697(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 285-761.
  5. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-282.
    Strain: C57BL/6.
    Tissue: Plasma.
  6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-119.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiCFAB_MOUSE
AccessioniPrimary (citable) accession number: P04186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3