Reviewed, UniProtKB/Swiss-Prot P04186 (CFAB_MOUSE)

Names and origin

Protein names

Recommended name:
    Complement factor B
    EC=3.4.21.47
Alternative name(s):
    C3/C5 convertase
Cleaved into the following 2 chains:
    1- Recommended name:
            Complement factor B Ba fragment
    2- Recommended name:
            Complement factor B Bb fragment

Gene names

Name:	Cfb
Synonyms:	Bf, H2-Bf

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
Catalytic activity	Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 3 Sushi (CCP/SCR) domains. Contains 1 VWFA domain.

Ontologies

Keywords
Biological process	Complement alternate pathway Immune response Innate immunity
Cellular component	Secreted
Domain	Repeat Signal Sushi
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	cell proliferation Traceable author statement. Source: MGI complement activation, alternative pathway Inferred from mutant phenotype. Source: MGI proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 761	739	Complement factor B		PRO_0000027548
	Chain	23 – 256	234	Complement factor B Ba fragment		PRO_0000027549
	Chain	257 – 761	505	Complement factor B Bb fragment		PRO_0000027550
Regions
	Domain	32 – 97	66	Sushi 1
	Domain	98 – 157	60	Sushi 2
	Domain	160 – 217	58	Sushi 3
	Domain	267 – 466	200	VWFA
	Domain	474 – 754	281	Peptidase S1
Sites
	Active site	523	1	Charge relay system
	Active site	573	1	Charge relay system
	Active site	696	1	Charge relay system
Amino acid modifications
	Glycosylation	119	1	N-linked (GlcNAc...) Ref.6
	Glycosylation	139	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
	Disulfide bond	34 ↔ 73		By similarity
	Disulfide bond	59 ↔ 95		By similarity
	Disulfide bond	100 ↔ 142		By similarity
	Disulfide bond	128 ↔ 155		By similarity
	Disulfide bond	162 ↔ 202		By similarity
	Disulfide bond	188 ↔ 215		By similarity
	Disulfide bond	508 ↔ 524		By similarity
	Disulfide bond	692 ↔ 722		By similarity
Experimental info
	Sequence conflict	730	1	L → P in AAA39549. Ref.4
	Sequence conflict	745	1	V → E in AAA39549. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P04186-1 [UniParc]. Last modified August 1, 1991. Version 2. Checksum: 9E9015448A61020E	FASTA	761	85,004
	10 20 30 40 50 60 MESPQLCLVL LVLGFSSGGV SATPVLEARP QVSCSLEGVE IKGGSFQLLQ GGQALEYLCP 70 80 90 100 110 120 SGFYPYPVQT RTCRSTGSWS DLQTRDQKIV QKAECRAIRC PRPQDFENGE FWPRSPFYNL 130 140 150 160 170 180 SDQISFQCYD GYVLRGSANR TCQENGRWDG QTAICDDGAG YCPNPGIPIG TRKVGSQYRL 190 200 210 220 230 240 EDIVTYHCSR GLVLRGSQKR KCQEGGSWSG TEPSCQDSFM YDSPQEVAEA FLSSLTETIE 250 260 270 280 290 300 GADAEDGHSP GEQQKRKIVL DPSGSMNIYL VLDGSDSIGS SNFTGAKRCL TNLIEKVASY 310 320 330 340 350 360 GVRPRYGLLT YATVPKVLVR VSDERSSDAD WVTEKLNQIS YEDHKLKSGT NTKRALQAVY 370 380 390 400 410 420 SMMSWAGDAP PEGWNRTRHV IIIMTDGLHN MGGNPVTVIQ DIRALLDIGR DPKNPREDYL 430 440 450 460 470 480 DVYVFGVGPL VDSVNINALA SKKDNEHHVF KVKDMEDLEN VFYQMIDETK SLSLCGMVWE 490 500 510 520 530 540 HKKGNDYHKQ PWQAKISVTR PLKGHETCMG AVVSEYFVLT AAHCFMVDDQ KHSIKVSVGG 550 560 570 580 590 600 QRRDLEIEEV LFHPKYNING KKAEGIPEFY DYDVALVKLK NKLKYGQTLR PICLPCTEGT 610 620 630 640 650 660 TRALRLPQTA TCKQHKEQLL PVKDVKALFV SEQGKSLTRK EVYIKNGDKK ASCERDATKA 670 680 690 700 710 720 QGYEKVKDAS EVVTPRFLCT GGVDPYADPN TCKGDSGGPL IVHKRSRFIQ VGVISWGVVD 730 740 750 760 VCRDQRRQQL VPSYARDFHI NLFQVLPWLK DKLKDEDLGF L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Murine complement C2 and factor B genomic and cDNA cloning reveals different mechanisms for multiple transcripts of C2 and B." Ishikawa N., Nonaka M., Wetsel R.A., Colten H.R. J. Biol. Chem. 265:19040-19046(1990) [PubMed: 2229060] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed: 14656967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[4]	"Phylogenetic conservation of a class III major histocompatibility complex antigen, factor B. Isolation and nucleotide sequencing of mouse factor B cDNA clones." Sackstein R., Colten H.R., Woods D.E. J. Biol. Chem. 258:14693-14697(1983) [PubMed: 6689022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 285-761.
[5]	"Proteome-wide characterization of N-glycosylation events by diagonal chromatography." Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K. J. Proteome Res. 5:2438-2447(2006) [PubMed: 16944957] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-282, MASS SPECTROMETRY. Tissue: Plasma.
[6]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-119, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60646 , M60629, M60630, M60631, M60632, M60633, M60634, M60635, M60636, M60637, M60638, M60639, M60640, M60641, M60642, M60643, M60644, M60645 Genomic DNA. Translation: AAA37379.1. AF049850 Genomic DNA. Translation: AAC05283.1. AF109906 Genomic DNA. Translation: AAC84160.1. BC005451 mRNA. Translation: AAH05451.1. K01496 mRNA. Translation: AAA39549.1. K01497 mRNA. Translation: AAA39550.1. K01498 mRNA. Translation: AAA39551.1. M57890 mRNA. Translation: AAA63293.1.
IPI	IPI00114065.
PIR	BBMS. A38875.
UniGene	Mm.653
3D structure databases
HSSP	HSSP built from PDB template 1H03 based on UniProtKB P08174.
SMR	P04186. Positions 31-761.
ModBase	Search...
Proteomic databases
PRIDE	P04186.
Genome annotation databases
Ensembl	ENSMUSG00000024371. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:105975. Cfb.
Phylogenomic databases
HOGENOM	P04186.
HOVERGEN	P04186.
OMA	P04186. ECRAIRC.
Enzyme and pathway databases
BRENDA	3.4.21.47. 244.
Gene expression databases
Bgee	P04186.
CleanEx	MM_CFB.
GermOnline	ENSMUSG00000024371. Mus musculus.
Family and domain databases
InterPro	IPR011360. Compl_C2_B. IPR016060. Complement_control_module. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR000436. Sushi_SCR_CCP. IPR002035. VWF_A. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 2 hits.
Pfam	PF00084. Sushi. 2 hits. PF00089. Trypsin. 1 hit. PF00092. VWA. 1 hit. [Graphical view]
PIRSF	PIRSF001154. Compl_C2_B. 1 hit.
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00032. CCP. 3 hits. SM00020. Tryp_SPc. 1 hit. SM00327. VWA. 1 hit. [Graphical view]
PROSITE	PS50923. SUSHI. 3 hits. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. PS50234. VWFA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	287314.
SOURCE	Search...

Entry information

Entry name

CFAB_MOUSE

Accession

Primary (citable) accession number: P04186

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)