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P04186

- CFAB_MOUSE

UniProt

P04186 - CFAB_MOUSE

Protein

Complement factor B

Gene

Cfb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 2 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.

    Catalytic activityi

    Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei523 – 5231Charge relay system
    Active sitei573 – 5731Charge relay system
    Active sitei696 – 6961Charge relay system

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. cell proliferation Source: MGI
    2. complement activation, alternative pathway Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Complement alternate pathway, Immunity, Innate immunity

    Enzyme and pathway databases

    BRENDAi3.4.21.47. 3474.
    ReactomeiREACT_198562. Regulation of Complement cascade.
    REACT_213087. Activation of C3 and C5.
    REACT_218037. Alternative complement activation.

    Protein family/group databases

    MEROPSiS01.196.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Complement factor B (EC:3.4.21.47)
    Alternative name(s):
    C3/C5 convertase
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Cfb
    Synonyms:Bf, H2-Bf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:105975. Cfb.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222By similarityAdd
    BLAST
    Chaini23 – 761739Complement factor BPRO_0000027548Add
    BLAST
    Chaini23 – 256234Complement factor B Ba fragmentPRO_0000027549Add
    BLAST
    Chaini257 – 761505Complement factor B Bb fragmentPRO_0000027550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi34 ↔ 73By similarity
    Disulfide bondi59 ↔ 95By similarity
    Disulfide bondi100 ↔ 142By similarity
    Glycosylationi119 – 1191N-linked (GlcNAc...)1 Publication
    Disulfide bondi128 ↔ 155By similarity
    Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi162 ↔ 202By similarity
    Disulfide bondi188 ↔ 215By similarity
    Glycosylationi282 – 2821N-linked (GlcNAc...)1 Publication
    Glycosylationi375 – 3751N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi508 ↔ 524By similarity
    Disulfide bondi692 ↔ 722By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP04186.
    PaxDbiP04186.
    PRIDEiP04186.

    PTM databases

    PhosphoSiteiP04186.

    Expressioni

    Gene expression databases

    BgeeiP04186.
    CleanExiMM_CFB.
    GenevestigatoriP04186.

    Interactioni

    Protein-protein interaction databases

    IntActiP04186. 1 interaction.
    MINTiMINT-1865229.
    STRINGi10090.ENSMUSP00000117677.

    Structurei

    3D structure databases

    ProteinModelPortaliP04186.
    SMRiP04186. Positions 33-761.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 9766Sushi 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini98 – 15760Sushi 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini160 – 21758Sushi 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini267 – 466200VWFAPROSITE-ProRule annotationAdd
    BLAST
    Domaini474 – 754281Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The unliganded VWA domain has an inactive 'locked' conformation whereby the scissile Arg-256|Lys-257 bond is protected from proteolytic activation.By similarity

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 3 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Sushi

    Phylogenomic databases

    eggNOGiNOG306397.
    HOGENOMiHOG000038034.
    HOVERGENiHBG002567.
    InParanoidiP04186.
    OrthoDBiEOG71CFK5.
    PhylomeDBiP04186.

    Family and domain databases

    Gene3Di3.40.50.410. 1 hit.
    InterProiIPR011360. Compl_C2_B.
    IPR028341. Complement_B.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000436. Sushi_SCR_CCP.
    IPR009003. Trypsin-like_Pept_dom.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF00084. Sushi. 3 hits.
    PF00089. Trypsin. 1 hit.
    PF00092. VWA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001154. Compl_C2_B. 1 hit.
    PIRSF500181. Complement_B. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00032. CCP. 3 hits.
    SM00020. Tryp_SPc. 1 hit.
    SM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF53300. SSF53300. 1 hit.
    SSF57535. SSF57535. 3 hits.
    PROSITEiPS50923. SUSHI. 3 hits.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    PS50234. VWFA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04186-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESPQLCLVL LVLGFSSGGV SATPVLEARP QVSCSLEGVE IKGGSFQLLQ    50
    GGQALEYLCP SGFYPYPVQT RTCRSTGSWS DLQTRDQKIV QKAECRAIRC 100
    PRPQDFENGE FWPRSPFYNL SDQISFQCYD GYVLRGSANR TCQENGRWDG 150
    QTAICDDGAG YCPNPGIPIG TRKVGSQYRL EDIVTYHCSR GLVLRGSQKR 200
    KCQEGGSWSG TEPSCQDSFM YDSPQEVAEA FLSSLTETIE GADAEDGHSP 250
    GEQQKRKIVL DPSGSMNIYL VLDGSDSIGS SNFTGAKRCL TNLIEKVASY 300
    GVRPRYGLLT YATVPKVLVR VSDERSSDAD WVTEKLNQIS YEDHKLKSGT 350
    NTKRALQAVY SMMSWAGDAP PEGWNRTRHV IIIMTDGLHN MGGNPVTVIQ 400
    DIRALLDIGR DPKNPREDYL DVYVFGVGPL VDSVNINALA SKKDNEHHVF 450
    KVKDMEDLEN VFYQMIDETK SLSLCGMVWE HKKGNDYHKQ PWQAKISVTR 500
    PLKGHETCMG AVVSEYFVLT AAHCFMVDDQ KHSIKVSVGG QRRDLEIEEV 550
    LFHPKYNING KKAEGIPEFY DYDVALVKLK NKLKYGQTLR PICLPCTEGT 600
    TRALRLPQTA TCKQHKEQLL PVKDVKALFV SEQGKSLTRK EVYIKNGDKK 650
    ASCERDATKA QGYEKVKDAS EVVTPRFLCT GGVDPYADPN TCKGDSGGPL 700
    IVHKRSRFIQ VGVISWGVVD VCRDQRRQQL VPSYARDFHI NLFQVLPWLK 750
    DKLKDEDLGF L 761
    Length:761
    Mass (Da):85,004
    Last modified:August 1, 1991 - v2
    Checksum:i9E9015448A61020E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti730 – 7301L → P in AAA39549. (PubMed:6689022)Curated
    Sequence conflicti745 – 7451V → E in AAA39549. (PubMed:6689022)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60646
    , M60629, M60630, M60631, M60632, M60633, M60634, M60635, M60636, M60637, M60638, M60639, M60640, M60641, M60642, M60643, M60644, M60645 Genomic DNA. Translation: AAA37379.1.
    AF049850 Genomic DNA. Translation: AAC05283.1.
    AF109906 Genomic DNA. Translation: AAC84160.1.
    BC005451 mRNA. Translation: AAH05451.1.
    K01496 mRNA. Translation: AAA39549.1.
    K01497 mRNA. Translation: AAA39550.1.
    K01498 mRNA. Translation: AAA39551.1.
    M57890 mRNA. Translation: AAA63293.1.
    PIRiA38875. BBMS.
    UniGeneiMm.653.

    Genome annotation databases

    EnsembliENSMUST00000128767; ENSMUSP00000119977; ENSMUSG00000090231.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60646
    , M60629 , M60630 , M60631 , M60632 , M60633 , M60634 , M60635 , M60636 , M60637 , M60638 , M60639 , M60640 , M60641 , M60642 , M60643 , M60644 , M60645 Genomic DNA. Translation: AAA37379.1 .
    AF049850 Genomic DNA. Translation: AAC05283.1 .
    AF109906 Genomic DNA. Translation: AAC84160.1 .
    BC005451 mRNA. Translation: AAH05451.1 .
    K01496 mRNA. Translation: AAA39549.1 .
    K01497 mRNA. Translation: AAA39550.1 .
    K01498 mRNA. Translation: AAA39551.1 .
    M57890 mRNA. Translation: AAA63293.1 .
    PIRi A38875. BBMS.
    UniGenei Mm.653.

    3D structure databases

    ProteinModelPortali P04186.
    SMRi P04186. Positions 33-761.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P04186. 1 interaction.
    MINTi MINT-1865229.
    STRINGi 10090.ENSMUSP00000117677.

    Protein family/group databases

    MEROPSi S01.196.

    PTM databases

    PhosphoSitei P04186.

    Proteomic databases

    MaxQBi P04186.
    PaxDbi P04186.
    PRIDEi P04186.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000128767 ; ENSMUSP00000119977 ; ENSMUSG00000090231 .

    Organism-specific databases

    MGIi MGI:105975. Cfb.

    Phylogenomic databases

    eggNOGi NOG306397.
    HOGENOMi HOG000038034.
    HOVERGENi HBG002567.
    InParanoidi P04186.
    OrthoDBi EOG71CFK5.
    PhylomeDBi P04186.

    Enzyme and pathway databases

    BRENDAi 3.4.21.47. 3474.
    Reactomei REACT_198562. Regulation of Complement cascade.
    REACT_213087. Activation of C3 and C5.
    REACT_218037. Alternative complement activation.

    Miscellaneous databases

    PROi P04186.
    SOURCEi Search...

    Gene expression databases

    Bgeei P04186.
    CleanExi MM_CFB.
    Genevestigatori P04186.

    Family and domain databases

    Gene3Di 3.40.50.410. 1 hit.
    InterProi IPR011360. Compl_C2_B.
    IPR028341. Complement_B.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000436. Sushi_SCR_CCP.
    IPR009003. Trypsin-like_Pept_dom.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF00084. Sushi. 3 hits.
    PF00089. Trypsin. 1 hit.
    PF00092. VWA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001154. Compl_C2_B. 1 hit.
    PIRSF500181. Complement_B. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00032. CCP. 3 hits.
    SM00020. Tryp_SPc. 1 hit.
    SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF53300. SSF53300. 1 hit.
    SSF57535. SSF57535. 3 hits.
    PROSITEi PS50923. SUSHI. 3 hits.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine complement C2 and factor B genomic and cDNA cloning reveals different mechanisms for multiple transcripts of C2 and B."
      Ishikawa N., Nonaka M., Wetsel R.A., Colten H.R.
      J. Biol. Chem. 265:19040-19046(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
      Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
      Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    4. "Phylogenetic conservation of a class III major histocompatibility complex antigen, factor B. Isolation and nucleotide sequencing of mouse factor B cDNA clones."
      Sackstein R., Colten H.R., Woods D.E.
      J. Biol. Chem. 258:14693-14697(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 285-761.
    5. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-282.
      Strain: C57BL/6.
      Tissue: Plasma.
    6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-119.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiCFAB_MOUSE
    AccessioniPrimary (citable) accession number: P04186
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 147 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3