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P04185 (UROK_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Urokinase-type plasminogen activator
Short name=U-plasminogen activator
Short name=uPA
EC=3.4.21.73

Cleaved into the following 3 chains:

  1. Urokinase-type plasminogen activator long chain A
  2. Urokinase-type plasminogen activator short chain A
  3. Urokinase-type plasminogen activator chain B
Gene names
Name:PLAU
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activity

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulation

Inhibited by SERPINA5 By similarity.

Subunit structure

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 EGF-like domain.

Contains 1 kringle domain.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processPlasminogen activation
   Cellular componentSecreted
   DomainEGF-like domain
Kringle
Signal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 By similarity
Chain21 – 442422Urokinase-type plasminogen activator
PRO_0000028330
Chain21 – 188168Urokinase-type plasminogen activator long chain A By similarity
PRO_0000028331
Chain167 – 18822Urokinase-type plasminogen activator short chain A By similarity
PRO_0000285900
Chain190 – 442253Urokinase-type plasminogen activator chain B By similarity
PRO_0000028332

Regions

Domain29 – 6537EGF-like
Domain72 – 15382Kringle
Domain190 – 435246Peptidase S1
Region36 – 5924Binds urokinase plasminogen activator surface receptor By similarity
Region154 – 18936Connecting peptide

Sites

Active site2351Charge relay system
Active site2861Charge relay system
Active site3871Charge relay system

Amino acid modifications

Glycosylation1521N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 41 By similarity
Disulfide bond35 ↔ 53 By similarity
Disulfide bond55 ↔ 64 By similarity
Disulfide bond72 ↔ 153 By similarity
Disulfide bond93 ↔ 135 By similarity
Disulfide bond124 ↔ 148 By similarity
Disulfide bond179 ↔ 310Interchain (between A and B chains) By similarity
Disulfide bond220 ↔ 236 By similarity
Disulfide bond228 ↔ 299 By similarity
Disulfide bond324 ↔ 393 By similarity
Disulfide bond356 ↔ 372 By similarity
Disulfide bond383 ↔ 411 By similarity

Experimental info

Sequence conflict2411Q → H in CAA25806. Ref.1
Sequence conflict2421Q → H in CAA26511. Ref.1
Sequence conflict2881A → GS in CAA25806. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P04185 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: EE32FCEF501321EE

FASTA44249,117
        10         20         30         40         50         60 
MRVLRACLSL CVLVVSDSKG SHELHQESGA SNCGCLNGGK CVSYKYFSNI QRCSCPKKFQ 

        70         80         90        100        110        120 
GEHCEIDTSQ TCFEGNGHSY RGKANTNTGG RPCLPWNSAT VLLNTYHAHR PDALQLGLGK 

       130        140        150        160        170        180 
HNYCRNPDNQ RRPWCYVQVG LKQLVQECMV PNCSGGESHR PAYDGKNPFS TPEKVEFQCG 

       190        200        210        220        230        240 
QKALRPRFKI VGGKSTTIEN QPWFAAIYRR HRGGSVTYVC GGSLISPCWV VSATHCFINY 

       250        260        270        280        290        300 
QQKEDYIVYL GRQTLHSSTH GEMKFEVEKL ILHEDYSADS LAHHNDIALL KIRTDKGQCA 

       310        320        330        340        350        360 
QPSRSIQTIC LPPVNGDAHF GASCEIVGFG KEDPSDYLYP EQLKMTVVKL VSHRECQQPH 

       370        380        390        400        410        420 
YYGSEVTTKM LCAADPQWKT DSCQGDSGGP LVCSTQGRLT LTGIVSWGRE CAMKDKPGVY 

       430        440 
TRVSRFLTWI HTHVGGENGL AH

« Hide

References

[1]"cDNA and gene nucleotide sequence of porcine plasminogen activator."
Nagamine Y., Pearson D., Altus M.S., Reich E.
Nucleic Acids Res. 12:9525-9541(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Kidney.
[2]Nagamine Y.
Submitted (DEC-1986) to the PIR data bank
Cited for: SEQUENCE REVISION TO 241.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X01648 Genomic DNA. Translation: CAA25806.1.
X02724 mRNA. Translation: CAA26511.1.
PIRUKPG. A00932.
RefSeqNP_999110.1. NM_213945.1.
UniGeneSsc.11194.

3D structure databases

ProteinModelPortalP04185.
SMRP04185. Positions 31-157, 177-437.
ModBaseSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000010995.

Protein family/group databases

MEROPSS01.231.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396985.
KEGGssc:396985.

Organism-specific databases

CTD5328.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000237314.
HOVERGENHBG008633.
KOK01348.
OrthoDBEOG4229K6.

Family and domain databases

Gene3D2.40.20.10. 1 hit.
InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF57440. Kringle-like. 1 hit.
SSF50494. Pept_Ser_Cys. 1 hit.
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChEMBLCHEMBL1075030.

Entry information

Entry nameUROK_PIG
AccessionPrimary (citable) accession number: P04185
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 13, 1987
Last modified: April 3, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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