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P04185

- UROK_PIG

UniProt

P04185 - UROK_PIG

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Protein
Urokinase-type plasminogen activator
Gene
PLAU
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Enzyme regulationi

Inhibited by SERPINA5 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei235 – 2351Charge relay system
Active sitei286 – 2861Charge relay system
Active sitei387 – 3871Charge relay system

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Plasminogen activation

Protein family/group databases

MEROPSiS01.231.

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 3 chains:
Gene namesi
Name:PLAU
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 By similarity
Add
BLAST
Chaini21 – 442422Urokinase-type plasminogen activator
PRO_0000028330Add
BLAST
Chaini21 – 188168Urokinase-type plasminogen activator long chain A By similarity
PRO_0000028331Add
BLAST
Chaini167 – 18822Urokinase-type plasminogen activator short chain A By similarity
PRO_0000285900Add
BLAST
Chaini190 – 442253Urokinase-type plasminogen activator chain B By similarity
PRO_0000028332Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 41 By similarity
Disulfide bondi35 ↔ 53 By similarity
Disulfide bondi55 ↔ 64 By similarity
Disulfide bondi72 ↔ 153 By similarity
Disulfide bondi93 ↔ 135 By similarity
Disulfide bondi124 ↔ 148 By similarity
Glycosylationi152 – 1521N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi179 ↔ 310Interchain (between A and B chains) By similarity
Disulfide bondi220 ↔ 236 By similarity
Disulfide bondi228 ↔ 299 By similarity
Disulfide bondi324 ↔ 393 By similarity
Disulfide bondi356 ↔ 372 By similarity
Disulfide bondi383 ↔ 411 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Interactioni

Subunit structurei

Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010995.

Structurei

3D structure databases

ProteinModelPortaliP04185.
SMRiP04185. Positions 31-157, 177-437.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 6537EGF-like
Add
BLAST
Domaini72 – 15382Kringle
Add
BLAST
Domaini190 – 435246Peptidase S1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 5924Binds urokinase plasminogen activator surface receptor By similarity
Add
BLAST
Regioni154 – 18936Connecting peptide
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000237314.
HOVERGENiHBG008633.
KOiK01348.

Family and domain databases

Gene3Di2.40.20.10. 1 hit.
InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04185-1 [UniParc]FASTAAdd to Basket

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MRVLRACLSL CVLVVSDSKG SHELHQESGA SNCGCLNGGK CVSYKYFSNI    50
QRCSCPKKFQ GEHCEIDTSQ TCFEGNGHSY RGKANTNTGG RPCLPWNSAT 100
VLLNTYHAHR PDALQLGLGK HNYCRNPDNQ RRPWCYVQVG LKQLVQECMV 150
PNCSGGESHR PAYDGKNPFS TPEKVEFQCG QKALRPRFKI VGGKSTTIEN 200
QPWFAAIYRR HRGGSVTYVC GGSLISPCWV VSATHCFINY QQKEDYIVYL 250
GRQTLHSSTH GEMKFEVEKL ILHEDYSADS LAHHNDIALL KIRTDKGQCA 300
QPSRSIQTIC LPPVNGDAHF GASCEIVGFG KEDPSDYLYP EQLKMTVVKL 350
VSHRECQQPH YYGSEVTTKM LCAADPQWKT DSCQGDSGGP LVCSTQGRLT 400
LTGIVSWGRE CAMKDKPGVY TRVSRFLTWI HTHVGGENGL AH 442
Length:442
Mass (Da):49,117
Last modified:August 13, 1987 - v1
Checksum:iEE32FCEF501321EE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti241 – 2411Q → H in CAA25806. 1 Publication
Sequence conflicti242 – 2421Q → H in CAA26511. 1 Publication
Sequence conflicti288 – 2881A → GS in CAA25806. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01648 Genomic DNA. Translation: CAA25806.1.
X02724 mRNA. Translation: CAA26511.1.
PIRiA00932. UKPG.
RefSeqiNP_999110.1. NM_213945.1.
UniGeneiSsc.11194.

Genome annotation databases

GeneIDi396985.
KEGGissc:396985.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X01648 Genomic DNA. Translation: CAA25806.1 .
X02724 mRNA. Translation: CAA26511.1 .
PIRi A00932. UKPG.
RefSeqi NP_999110.1. NM_213945.1.
UniGenei Ssc.11194.

3D structure databases

ProteinModelPortali P04185.
SMRi P04185. Positions 31-157, 177-437.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000010995.

Chemistry

ChEMBLi CHEMBL1075030.

Protein family/group databases

MEROPSi S01.231.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396985.
KEGGi ssc:396985.

Organism-specific databases

CTDi 5328.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000237314.
HOVERGENi HBG008633.
KOi K01348.

Family and domain databases

Gene3Di 2.40.20.10. 1 hit.
InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA and gene nucleotide sequence of porcine plasminogen activator."
    Nagamine Y., Pearson D., Altus M.S., Reich E.
    Nucleic Acids Res. 12:9525-9541(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Kidney.
  2. Nagamine Y.
    Submitted (DEC-1986) to the PIR data bank
    Cited for: SEQUENCE REVISION TO 241.

Entry informationi

Entry nameiUROK_PIG
AccessioniPrimary (citable) accession number: P04185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 13, 1987
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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