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P04185

- UROK_PIG

UniProt

P04185 - UROK_PIG

Protein

Urokinase-type plasminogen activator

Gene

PLAU

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

    Catalytic activityi

    Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

    Enzyme regulationi

    Inhibited by SERPINA5.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei235 – 2351Charge relay system
    Active sitei286 – 2861Charge relay system
    Active sitei387 – 3871Charge relay system

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Plasminogen activation

    Protein family/group databases

    MEROPSiS01.231.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urokinase-type plasminogen activator (EC:3.4.21.73)
    Short name:
    U-plasminogen activator
    Short name:
    uPA
    Cleaved into the following 3 chains:
    Gene namesi
    Name:PLAU
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020By similarityAdd
    BLAST
    Chaini21 – 442422Urokinase-type plasminogen activatorPRO_0000028330Add
    BLAST
    Chaini21 – 188168Urokinase-type plasminogen activator long chain ABy similarityPRO_0000028331Add
    BLAST
    Chaini167 – 18822Urokinase-type plasminogen activator short chain ABy similarityPRO_0000285900Add
    BLAST
    Chaini190 – 442253Urokinase-type plasminogen activator chain BBy similarityPRO_0000028332Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 41By similarity
    Disulfide bondi35 ↔ 53By similarity
    Disulfide bondi55 ↔ 64By similarity
    Disulfide bondi72 ↔ 153By similarity
    Disulfide bondi93 ↔ 135By similarity
    Disulfide bondi124 ↔ 148By similarity
    Glycosylationi152 – 1521N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi179 ↔ 310Interchain (between A and B chains)PROSITE-ProRule annotation
    Disulfide bondi220 ↔ 236By similarity
    Disulfide bondi228 ↔ 299By similarity
    Disulfide bondi324 ↔ 393By similarity
    Disulfide bondi356 ↔ 372By similarity
    Disulfide bondi383 ↔ 411By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Interactioni

    Subunit structurei

    Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Forms heterodimer with SERPINA5. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000010995.

    Structurei

    3D structure databases

    ProteinModelPortaliP04185.
    SMRiP04185. Positions 31-157, 177-437.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 6537EGF-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini72 – 15382KringlePROSITE-ProRule annotationAdd
    BLAST
    Domaini190 – 435246Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 5924Binds urokinase plasminogen activator surface receptorBy similarityAdd
    BLAST
    Regioni154 – 18936Connecting peptideAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 1 kringle domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Kringle, Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000237314.
    HOVERGENiHBG008633.
    KOiK01348.

    Family and domain databases

    Gene3Di2.40.20.10. 1 hit.
    InterProiIPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04185-1 [UniParc]FASTAAdd to Basket

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    MRVLRACLSL CVLVVSDSKG SHELHQESGA SNCGCLNGGK CVSYKYFSNI    50
    QRCSCPKKFQ GEHCEIDTSQ TCFEGNGHSY RGKANTNTGG RPCLPWNSAT 100
    VLLNTYHAHR PDALQLGLGK HNYCRNPDNQ RRPWCYVQVG LKQLVQECMV 150
    PNCSGGESHR PAYDGKNPFS TPEKVEFQCG QKALRPRFKI VGGKSTTIEN 200
    QPWFAAIYRR HRGGSVTYVC GGSLISPCWV VSATHCFINY QQKEDYIVYL 250
    GRQTLHSSTH GEMKFEVEKL ILHEDYSADS LAHHNDIALL KIRTDKGQCA 300
    QPSRSIQTIC LPPVNGDAHF GASCEIVGFG KEDPSDYLYP EQLKMTVVKL 350
    VSHRECQQPH YYGSEVTTKM LCAADPQWKT DSCQGDSGGP LVCSTQGRLT 400
    LTGIVSWGRE CAMKDKPGVY TRVSRFLTWI HTHVGGENGL AH 442
    Length:442
    Mass (Da):49,117
    Last modified:August 13, 1987 - v1
    Checksum:iEE32FCEF501321EE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti241 – 2411Q → H in CAA25806. (PubMed:6096832)Curated
    Sequence conflicti242 – 2421Q → H in CAA26511. (PubMed:6096832)Curated
    Sequence conflicti288 – 2881A → GS in CAA25806. (PubMed:6096832)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01648 Genomic DNA. Translation: CAA25806.1.
    X02724 mRNA. Translation: CAA26511.1.
    PIRiA00932. UKPG.
    RefSeqiNP_999110.1. NM_213945.1.
    UniGeneiSsc.11194.

    Genome annotation databases

    GeneIDi396985.
    KEGGissc:396985.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01648 Genomic DNA. Translation: CAA25806.1 .
    X02724 mRNA. Translation: CAA26511.1 .
    PIRi A00932. UKPG.
    RefSeqi NP_999110.1. NM_213945.1.
    UniGenei Ssc.11194.

    3D structure databases

    ProteinModelPortali P04185.
    SMRi P04185. Positions 31-157, 177-437.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000010995.

    Chemistry

    ChEMBLi CHEMBL1075030.

    Protein family/group databases

    MEROPSi S01.231.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396985.
    KEGGi ssc:396985.

    Organism-specific databases

    CTDi 5328.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000237314.
    HOVERGENi HBG008633.
    KOi K01348.

    Family and domain databases

    Gene3Di 2.40.20.10. 1 hit.
    InterProi IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000001. Kringle.
    IPR013806. Kringle-like.
    IPR018056. Kringle_CS.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00051. Kringle. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00130. KR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF57440. SSF57440. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS50026. EGF_3. 1 hit.
    PS00021. KRINGLE_1. 1 hit.
    PS50070. KRINGLE_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA and gene nucleotide sequence of porcine plasminogen activator."
      Nagamine Y., Pearson D., Altus M.S., Reich E.
      Nucleic Acids Res. 12:9525-9541(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Kidney.
    2. Nagamine Y.
      Submitted (DEC-1986) to the PIR data bank
      Cited for: SEQUENCE REVISION TO 241.

    Entry informationi

    Entry nameiUROK_PIG
    AccessioniPrimary (citable) accession number: P04185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3