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P04184

- KITH_MOUSE

UniProt

P04184 - KITH_MOUSE

Protein

Thymidine kinase, cytosolic

Gene

Tk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei98 – 981Proton acceptorSequence Analysis
    Binding sitei128 – 1281Substrate; via amide nitrogenBy similarity
    Metal bindingi153 – 1531ZincBy similarity
    Metal bindingi156 – 1561ZincBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Metal bindingi185 – 1851ZincBy similarity
    Metal bindingi188 – 1881ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338ATPCurated
    Nucleotide bindingi58 – 603ATPBy similarity
    Nucleotide bindingi97 – 1004ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. thymidine kinase activity Source: MGI
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
    2. digestive tract development Source: Ensembl
    3. DNA replication Source: UniProtKB-KW
    4. fetal process involved in parturition Source: Ensembl
    5. liver development Source: Ensembl
    6. protein homotetramerization Source: Ensembl
    7. response to copper ion Source: Ensembl
    8. response to cortisol Source: Ensembl
    9. response to nutrient levels Source: Ensembl
    10. response to toxic substance Source: Ensembl
    11. skeletal muscle cell proliferation Source: Ensembl
    12. thymidine metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.1.21. 3474.
    SABIO-RKP04184.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase, cytosolic (EC:2.7.1.21)
    Gene namesi
    Name:Tk1
    Synonyms:Tk-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:98763. Tk1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 233232Thymidine kinase, cytosolicPRO_0000174949Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on Ser-13 in mitosis.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP04184.
    PaxDbiP04184.
    PRIDEiP04184.

    PTM databases

    PhosphoSiteiP04184.

    Expressioni

    Gene expression databases

    ArrayExpressiP04184.
    BgeeiP04184.
    CleanExiMM_TK1.
    GenevestigatoriP04184.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP04184.
    SMRiP04184. Positions 18-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni172 – 1765Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1435.
    GeneTreeiENSGT00390000011309.
    HOGENOMiHOG000076390.
    HOVERGENiHBG006215.
    InParanoidiP04184.
    KOiK00857.
    OMAiQAICLSC.
    OrthoDBiEOG7MSMPX.
    PhylomeDBiP04184.
    TreeFamiTF314839.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04184-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYINLPTVL PSSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC    50
    LVIKYAKDTR YSNSFSTHDR NTMDALPACM LRDVTQESLG VAVIGIDEGQ 100
    FFPDIVDFCE MMANEGKTVI VAALDGTFQR KAFGSILNLV PLAESVVKLT 150
    AVCMECFREA AYTKRLGLEK EVEVIGGADK YHSVCRLCYF KKSSAQTAGS 200
    DNKNCLVLGQ PGEALVVRKL FASQQVLQYN SAN 233
    Length:233
    Mass (Da):25,776
    Last modified:February 26, 2008 - v3
    Checksum:i78366623EDED0C6C
    GO

    Sequence cautioni

    The sequence AAA40452.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti27 – 271P → L in AAH91758. (PubMed:15489334)Curated
    Sequence conflicti88 – 881S → L in AAA40451. (PubMed:3018504)Curated
    Sequence conflicti88 – 881S → L in AAA40452. (PubMed:3018504)Curated
    Sequence conflicti185 – 1851C → G in BAC39384. (PubMed:16141072)Curated
    Sequence conflicti198 – 1981A → V in BAC39384. (PubMed:16141072)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti88 – 881S → A.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11945 mRNA. Translation: AAA40451.1.
    M19438 mRNA. Translation: AAA40452.1. Different initiation.
    M68489 Genomic DNA. Translation: AAA40454.1.
    S57244 mRNA. Translation: AAB26001.1.
    AK085188 mRNA. Translation: BAC39384.1.
    AK087988 mRNA. Translation: BAC40077.1.
    AK139244 mRNA. Translation: BAE23931.1.
    BC012215 mRNA. Translation: AAH12215.1.
    BC091758 mRNA. Translation: AAH91758.1.
    X60980 Genomic DNA. Translation: CAA43296.1.
    M22581 Genomic DNA. Translation: AAA40450.1.
    CCDSiCCDS25692.1.
    PIRiJC1252. KIMST.
    RefSeqiNP_001258658.1. NM_001271729.1.
    NP_033413.2. NM_009387.2.
    UniGeneiMm.2661.

    Genome annotation databases

    EnsembliENSMUST00000026661; ENSMUSP00000026661; ENSMUSG00000025574.
    GeneIDi21877.
    KEGGimmu:21877.
    UCSCiuc007mnz.2. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11945 mRNA. Translation: AAA40451.1 .
    M19438 mRNA. Translation: AAA40452.1 . Different initiation.
    M68489 Genomic DNA. Translation: AAA40454.1 .
    S57244 mRNA. Translation: AAB26001.1 .
    AK085188 mRNA. Translation: BAC39384.1 .
    AK087988 mRNA. Translation: BAC40077.1 .
    AK139244 mRNA. Translation: BAE23931.1 .
    BC012215 mRNA. Translation: AAH12215.1 .
    BC091758 mRNA. Translation: AAH91758.1 .
    X60980 Genomic DNA. Translation: CAA43296.1 .
    M22581 Genomic DNA. Translation: AAA40450.1 .
    CCDSi CCDS25692.1.
    PIRi JC1252. KIMST.
    RefSeqi NP_001258658.1. NM_001271729.1.
    NP_033413.2. NM_009387.2.
    UniGenei Mm.2661.

    3D structure databases

    ProteinModelPortali P04184.
    SMRi P04184. Positions 18-191.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P04184.
    ChEMBLi CHEMBL2618.

    PTM databases

    PhosphoSitei P04184.

    Proteomic databases

    MaxQBi P04184.
    PaxDbi P04184.
    PRIDEi P04184.

    Protocols and materials databases

    DNASUi 21877.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026661 ; ENSMUSP00000026661 ; ENSMUSG00000025574 .
    GeneIDi 21877.
    KEGGi mmu:21877.
    UCSCi uc007mnz.2. mouse.

    Organism-specific databases

    CTDi 7083.
    MGIi MGI:98763. Tk1.

    Phylogenomic databases

    eggNOGi COG1435.
    GeneTreei ENSGT00390000011309.
    HOGENOMi HOG000076390.
    HOVERGENi HBG006215.
    InParanoidi P04184.
    KOi K00857.
    OMAi QAICLSC.
    OrthoDBi EOG7MSMPX.
    PhylomeDBi P04184.
    TreeFami TF314839.

    Enzyme and pathway databases

    BRENDAi 2.7.1.21. 3474.
    SABIO-RK P04184.

    Miscellaneous databases

    NextBioi 301386.
    PROi P04184.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04184.
    Bgeei P04184.
    CleanExi MM_TK1.
    Genevestigatori P04184.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences."
      Lin P.-F., Lieberman H.B., Yeh D.-B., Xu T., Zhao S.-Y., Ruddle F.H.
      Mol. Cell. Biol. 5:3149-3156(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Regulation of thymidine kinase during growth, cell cycle and differentiation."
      Wintersberger E., Rotheneder H., Grabner M., Beck G., Seiser C.
      Adv. Enzyme Regul. 32:241-254(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Characterization of the murine thymidine kinase-encoding gene and analysis of transcription start point heterogeneity."
      Gudas J.M., Fridovich-Keil J.L., Datta M.W., Bryan J., Pardee A.B.
      Gene 118:205-216(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-88.
    4. "Sequence analysis of tka(-)-1 and tkb(+)-1 alleles in L5178Y tk+/-mouse-lymphoma cells and spontaneous tk-/-mutants."
      Liechty M.C., Rauchfuss H.S., Lugo M.H., Hozier J.C.
      Mutat. Res. 286:299-307(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Cerebellum, Stomach and Thymus.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N and NMRI.
      Tissue: Mammary tumor.
    7. "Presence of regulatory sequences within intron 2 of the mouse thymidine kinase gene."
      Rotheneder H., Grabner M., Wintersberger E.
      Nucleic Acids Res. 19:6805-6809(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
      Tissue: Liver.
    8. "Transcriptional and posttranscriptional mechanisms regulate murine thymidine kinase gene expression in serum-stimulated cells."
      Lieberman H.B., Lin P.F., Yeh D.B., Ruddle F.H.
      Mol. Cell. Biol. 8:5280-5291(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.

    Entry informationi

    Entry nameiKITH_MOUSE
    AccessioniPrimary (citable) accession number: P04184
    Secondary accession number(s): Q58EU0, Q8C3P8, Q921J8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3