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P04184 (KITH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase, cytosolic

EC=2.7.1.21
Gene names
Name:Tk1
Synonyms:Tk-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Post-translational modification

Phosphorylated on Ser-13 in mitosis By similarity.

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Sequence similarities

Belongs to the thymidine kinase family.

Sequence caution

The sequence AAA40452.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processDNA synthesis
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

deoxyribonucleoside monophosphate biosynthetic process

Inferred from direct assay PubMed 204065. Source: GOC

digestive tract development

Inferred from electronic annotation. Source: Ensembl

fetal process involved in parturition

Inferred from electronic annotation. Source: Ensembl

liver development

Inferred from electronic annotation. Source: Ensembl

protein homotetramerization

Inferred from electronic annotation. Source: Ensembl

response to copper ion

Inferred from electronic annotation. Source: Ensembl

response to cortisol

Inferred from electronic annotation. Source: Ensembl

response to nutrient levels

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from electronic annotation. Source: Ensembl

skeletal muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

thymidine metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from direct assay PubMed 204065. Source: MGI

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 233232Thymidine kinase, cytosolic
PRO_0000174949

Regions

Nucleotide binding26 – 338ATP Probable
Nucleotide binding58 – 603ATP By similarity
Nucleotide binding97 – 1004ATP By similarity
Region172 – 1765Substrate binding By similarity

Sites

Active site981Proton acceptor Potential
Metal binding1531Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1851Zinc By similarity
Metal binding1881Zinc By similarity
Binding site1281Substrate; via amide nitrogen By similarity
Binding site1811Substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue131Phosphoserine By similarity

Natural variations

Natural variant881S → A. Ref.3

Experimental info

Sequence conflict271P → L in AAH91758. Ref.6
Sequence conflict881S → L in AAA40451. Ref.1
Sequence conflict881S → L in AAA40452. Ref.1
Sequence conflict1851C → G in BAC39384. Ref.5
Sequence conflict1981A → V in BAC39384. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P04184 [UniParc].

Last modified February 26, 2008. Version 3.
Checksum: 78366623EDED0C6C

FASTA23325,776
        10         20         30         40         50         60 
MSYINLPTVL PSSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC LVIKYAKDTR 

        70         80         90        100        110        120 
YSNSFSTHDR NTMDALPACM LRDVTQESLG VAVIGIDEGQ FFPDIVDFCE MMANEGKTVI 

       130        140        150        160        170        180 
VAALDGTFQR KAFGSILNLV PLAESVVKLT AVCMECFREA AYTKRLGLEK EVEVIGGADK 

       190        200        210        220        230 
YHSVCRLCYF KKSSAQTAGS DNKNCLVLGQ PGEALVVRKL FASQQVLQYN SAN 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences."
Lin P.-F., Lieberman H.B., Yeh D.-B., Xu T., Zhao S.-Y., Ruddle F.H.
Mol. Cell. Biol. 5:3149-3156(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Regulation of thymidine kinase during growth, cell cycle and differentiation."
Wintersberger E., Rotheneder H., Grabner M., Beck G., Seiser C.
Adv. Enzyme Regul. 32:241-254(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Characterization of the murine thymidine kinase-encoding gene and analysis of transcription start point heterogeneity."
Gudas J.M., Fridovich-Keil J.L., Datta M.W., Bryan J., Pardee A.B.
Gene 118:205-216(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-88.
[4]"Sequence analysis of tka(-)-1 and tkb(+)-1 alleles in L5178Y tk+/-mouse-lymphoma cells and spontaneous tk-/-mutants."
Liechty M.C., Rauchfuss H.S., Lugo M.H., Hozier J.C.
Mutat. Res. 286:299-307(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Cerebellum, Stomach and Thymus.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N and NMRI.
Tissue: Mammary tumor.
[7]"Presence of regulatory sequences within intron 2 of the mouse thymidine kinase gene."
Rotheneder H., Grabner M., Wintersberger E.
Nucleic Acids Res. 19:6805-6809(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
Tissue: Liver.
[8]"Transcriptional and posttranscriptional mechanisms regulate murine thymidine kinase gene expression in serum-stimulated cells."
Lieberman H.B., Lin P.F., Yeh D.B., Ruddle F.H.
Mol. Cell. Biol. 8:5280-5291(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11945 mRNA. Translation: AAA40451.1.
M19438 mRNA. Translation: AAA40452.1. Different initiation.
M68489 Genomic DNA. Translation: AAA40454.1.
S57244 mRNA. Translation: AAB26001.1.
AK085188 mRNA. Translation: BAC39384.1.
AK087988 mRNA. Translation: BAC40077.1.
AK139244 mRNA. Translation: BAE23931.1.
BC012215 mRNA. Translation: AAH12215.1.
BC091758 mRNA. Translation: AAH91758.1.
X60980 Genomic DNA. Translation: CAA43296.1.
M22581 Genomic DNA. Translation: AAA40450.1.
PIRKIMST. JC1252.
RefSeqNP_001258658.1. NM_001271729.1.
NP_033413.2. NM_009387.2.
UniGeneMm.2661.

3D structure databases

ProteinModelPortalP04184.
SMRP04184. Positions 18-191.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP04184.
ChEMBLCHEMBL2618.

PTM databases

PhosphoSiteP04184.

Proteomic databases

PaxDbP04184.
PRIDEP04184.

Protocols and materials databases

DNASU21877.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026661; ENSMUSP00000026661; ENSMUSG00000025574.
GeneID21877.
KEGGmmu:21877.
UCSCuc007mnz.2. mouse.

Organism-specific databases

CTD7083.
MGIMGI:98763. Tk1.

Phylogenomic databases

eggNOGCOG1435.
GeneTreeENSGT00390000011309.
HOGENOMHOG000076390.
HOVERGENHBG006215.
InParanoidP04184.
KOK00857.
OMAKVHAICV.
OrthoDBEOG7MSMPX.
PhylomeDBP04184.
TreeFamTF314839.

Enzyme and pathway databases

BRENDA2.7.1.21. 3474.
SABIO-RKP04184.

Gene expression databases

ArrayExpressP04184.
BgeeP04184.
CleanExMM_TK1.
GenevestigatorP04184.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301386.
PROP04184.
SOURCESearch...

Entry information

Entry nameKITH_MOUSE
AccessionPrimary (citable) accession number: P04184
Secondary accession number(s): Q58EU0, Q8C3P8, Q921J8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: February 26, 2008
Last modified: April 16, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot