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Protein

Thymidine kinase, cytosolic

Gene

Tk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Proton acceptorSequence analysis1
Binding sitei128Substrate; via amide nitrogenBy similarity1
Metal bindingi153ZincBy similarity1
Metal bindingi156ZincBy similarity1
Binding sitei181SubstrateBy similarity1
Metal bindingi185ZincBy similarity1
Metal bindingi188ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATPCurated8
Nucleotide bindingi58 – 60ATPBy similarity3
Nucleotide bindingi97 – 100ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • nucleoside kinase activity Source: MGI
  • thymidine kinase activity Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21. 3474.
ReactomeiR-MMU-73614. Pyrimidine salvage reactions.
SABIO-RKP04184.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:Tk1
Synonyms:Tk-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98763. Tk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2618.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001749492 – 233Thymidine kinase, cytosolicAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on Ser-13 in mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP04184.
MaxQBiP04184.
PaxDbiP04184.
PeptideAtlasiP04184.
PRIDEiP04184.

PTM databases

iPTMnetiP04184.
PhosphoSitePlusiP04184.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025574.
CleanExiMM_TK1.
GenevisibleiP04184. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026661.

Chemistry databases

BindingDBiP04184.

Structurei

3D structure databases

ProteinModelPortaliP04184.
SMRiP04184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 176Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiKOG3125. Eukaryota.
COG1435. LUCA.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiP04184.
KOiK00857.
OMAiVITGPMY.
OrthoDBiEOG091G0HZR.
PhylomeDBiP04184.
TreeFamiTF314839.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYINLPTVL PSSPSKTRGQ IQVILGPMFS GKSTELMRRV RRFQIAQYKC
60 70 80 90 100
LVIKYAKDTR YSNSFSTHDR NTMDALPACM LRDVTQESLG VAVIGIDEGQ
110 120 130 140 150
FFPDIVDFCE MMANEGKTVI VAALDGTFQR KAFGSILNLV PLAESVVKLT
160 170 180 190 200
AVCMECFREA AYTKRLGLEK EVEVIGGADK YHSVCRLCYF KKSSAQTAGS
210 220 230
DNKNCLVLGQ PGEALVVRKL FASQQVLQYN SAN
Length:233
Mass (Da):25,776
Last modified:February 26, 2008 - v3
Checksum:i78366623EDED0C6C
GO

Sequence cautioni

The sequence AAA40452 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27P → L in AAH91758 (PubMed:15489334).Curated1
Sequence conflicti88S → L in AAA40451 (PubMed:3018504).Curated1
Sequence conflicti88S → L in AAA40452 (PubMed:3018504).Curated1
Sequence conflicti185C → G in BAC39384 (PubMed:16141072).Curated1
Sequence conflicti198A → V in BAC39384 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti88S → A.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11945 mRNA. Translation: AAA40451.1.
M19438 mRNA. Translation: AAA40452.1. Different initiation.
M68489 Genomic DNA. Translation: AAA40454.1.
S57244 mRNA. Translation: AAB26001.1.
AK085188 mRNA. Translation: BAC39384.1.
AK087988 mRNA. Translation: BAC40077.1.
AK139244 mRNA. Translation: BAE23931.1.
BC012215 mRNA. Translation: AAH12215.1.
BC091758 mRNA. Translation: AAH91758.1.
X60980 Genomic DNA. Translation: CAA43296.1.
M22581 Genomic DNA. Translation: AAA40450.1.
CCDSiCCDS25692.1.
PIRiJC1252. KIMST.
RefSeqiNP_001258658.1. NM_001271729.1.
NP_033413.2. NM_009387.2.
UniGeneiMm.2661.
Mm.443968.

Genome annotation databases

EnsembliENSMUST00000026661; ENSMUSP00000026661; ENSMUSG00000025574.
GeneIDi21877.
KEGGimmu:21877.
UCSCiuc007mnz.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11945 mRNA. Translation: AAA40451.1.
M19438 mRNA. Translation: AAA40452.1. Different initiation.
M68489 Genomic DNA. Translation: AAA40454.1.
S57244 mRNA. Translation: AAB26001.1.
AK085188 mRNA. Translation: BAC39384.1.
AK087988 mRNA. Translation: BAC40077.1.
AK139244 mRNA. Translation: BAE23931.1.
BC012215 mRNA. Translation: AAH12215.1.
BC091758 mRNA. Translation: AAH91758.1.
X60980 Genomic DNA. Translation: CAA43296.1.
M22581 Genomic DNA. Translation: AAA40450.1.
CCDSiCCDS25692.1.
PIRiJC1252. KIMST.
RefSeqiNP_001258658.1. NM_001271729.1.
NP_033413.2. NM_009387.2.
UniGeneiMm.2661.
Mm.443968.

3D structure databases

ProteinModelPortaliP04184.
SMRiP04184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026661.

Chemistry databases

BindingDBiP04184.
ChEMBLiCHEMBL2618.

PTM databases

iPTMnetiP04184.
PhosphoSitePlusiP04184.

Proteomic databases

EPDiP04184.
MaxQBiP04184.
PaxDbiP04184.
PeptideAtlasiP04184.
PRIDEiP04184.

Protocols and materials databases

DNASUi21877.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026661; ENSMUSP00000026661; ENSMUSG00000025574.
GeneIDi21877.
KEGGimmu:21877.
UCSCiuc007mnz.2. mouse.

Organism-specific databases

CTDi7083.
MGIiMGI:98763. Tk1.

Phylogenomic databases

eggNOGiKOG3125. Eukaryota.
COG1435. LUCA.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiP04184.
KOiK00857.
OMAiVITGPMY.
OrthoDBiEOG091G0HZR.
PhylomeDBiP04184.
TreeFamiTF314839.

Enzyme and pathway databases

BRENDAi2.7.1.21. 3474.
ReactomeiR-MMU-73614. Pyrimidine salvage reactions.
SABIO-RKP04184.

Miscellaneous databases

PROiP04184.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025574.
CleanExiMM_TK1.
GenevisibleiP04184. MM.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_MOUSE
AccessioniPrimary (citable) accession number: P04184
Secondary accession number(s): Q58EU0, Q8C3P8, Q921J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: February 26, 2008
Last modified: November 30, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.