SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P04177

- TY3H_RAT

UniProt

P04177 - TY3H_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Tyrosine 3-monooxygenase

Gene
Th
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an important role in the physiology of adrenergic neurons.

Catalytic activityi

L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactori

Fe2+ ion.

Enzyme regulationi

Phosphorylation leads to an increase in the catalytic activity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi331 – 3311Iron
Metal bindingi336 – 3361Iron
Metal bindingi376 – 3761Iron

GO - Molecular functioni

  1. amino acid binding Source: RGD
  2. dopamine binding Source: RGD
  3. ferric iron binding Source: RGD
  4. ferrous iron binding Source: RGD
  5. monooxygenase activity Source: BHF-UCL
  6. oxygen binding Source: RGD
  7. protein binding Source: RGD
  8. protein domain specific binding Source: RGD
  9. tetrahydrobiopterin binding Source: RGD
  10. tyrosine 3-monooxygenase activity Source: RGD

GO - Biological processi

  1. catecholamine biosynthetic process Source: RGD
  2. cellular response to alkaloid Source: RGD
  3. cellular response to drug Source: RGD
  4. cellular response to glucose stimulus Source: RGD
  5. cellular response to growth factor stimulus Source: RGD
  6. cellular response to manganese ion Source: RGD
  7. cellular response to nicotine Source: RGD
  8. cerebral cortex development Source: RGD
  9. circadian sleep/wake cycle Source: RGD
  10. cognition Source: RGD
  11. dopamine biosynthetic process from tyrosine Source: BHF-UCL
  12. eating behavior Source: Ensembl
  13. embryonic camera-type eye morphogenesis Source: Ensembl
  14. epinephrine biosynthetic process Source: Ensembl
  15. eye photoreceptor cell development Source: Ensembl
  16. fatty acid metabolic process Source: RGD
  17. glycoside metabolic process Source: RGD
  18. heart development Source: RGD
  19. isoquinoline alkaloid metabolic process Source: RGD
  20. learning Source: Ensembl
  21. locomotory behavior Source: Ensembl
  22. mating behavior Source: Ensembl
  23. memory Source: Ensembl
  24. multicellular organismal aging Source: RGD
  25. neurotransmitter biosynthetic process Source: UniProtKB-KW
  26. norepinephrine biosynthetic process Source: Ensembl
  27. phthalate metabolic process Source: RGD
  28. phytoalexin metabolic process Source: RGD
  29. protein homotetramerization Source: RGD
  30. regulation of heart contraction Source: Ensembl
  31. response to activity Source: RGD
  32. response to amphetamine Source: RGD
  33. response to corticosterone Source: RGD
  34. response to drug Source: RGD
  35. response to electrical stimulus Source: RGD
  36. response to estradiol Source: RGD
  37. response to ethanol Source: RGD
  38. response to ether Source: RGD
  39. response to growth factor Source: RGD
  40. response to herbicide Source: RGD
  41. response to hypoxia Source: RGD
  42. response to immobilization stress Source: RGD
  43. response to insecticide Source: RGD
  44. response to light stimulus Source: RGD
  45. response to lipopolysaccharide Source: RGD
  46. response to metal ion Source: RGD
  47. response to nicotine Source: RGD
  48. response to nutrient levels Source: RGD
  49. response to organic cyclic compound Source: RGD
  50. response to peptide hormone Source: RGD
  51. response to pyrethroid Source: RGD
  52. response to salt stress Source: RGD
  53. response to steroid hormone Source: RGD
  54. response to water deprivation Source: RGD
  55. response to zinc ion Source: RGD
  56. sensory perception of sound Source: RGD
  57. social behavior Source: RGD
  58. sphingolipid metabolic process Source: RGD
  59. synaptic transmission, dopaminergic Source: Ensembl
  60. synaptic vesicle amine transport Source: RGD
  61. terpene metabolic process Source: RGD
  62. visual perception Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Catecholamine biosynthesis, Neurotransmitter biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP04177.
UniPathwayiUPA00747; UER00733.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine 3-monooxygenase (EC:1.14.16.2)
Alternative name(s):
Tyrosine 3-hydroxylase
Short name:
TH
Gene namesi
Name:Th
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3853. Th.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: RGD
  2. cytoplasm Source: RGD
  3. cytoplasmic side of plasma membrane Source: Ensembl
  4. cytoplasmic vesicle membrane Source: BHF-UCL
  5. cytosol Source: RGD
  6. dendrite Source: RGD
  7. melanosome membrane Source: Ensembl
  8. mitochondrion Source: BHF-UCL
  9. neuronal cell body Source: RGD
  10. neuron projection Source: RGD
  11. nucleus Source: Ensembl
  12. perikaryon Source: RGD
  13. smooth endoplasmic reticulum Source: Ensembl
  14. synaptic vesicle Source: RGD
  15. terminal bouton Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 498497Tyrosine 3-monooxygenasePRO_0000205564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Phosphoserine; by CaMK22 Publications
Modified residuei31 – 311Phosphoserine1 Publication
Modified residuei40 – 401Phosphoserine; by CaMK2 and PKA2 Publications

Post-translational modificationi

In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP04177.
PRIDEiP04177.

PTM databases

PhosphoSiteiP04177.

Expressioni

Gene expression databases

GenevestigatoriP04177.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi247158. 1 interaction.
IntActiP04177. 4 interactions.
MINTiMINT-4585867.

Structurei

Secondary structure

1
498
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi75 – 773
Beta strandi80 – 856
Beta strandi91 – 933
Helixi98 – 1069
Beta strandi110 – 1167
Beta strandi119 – 1224
Beta strandi133 – 1386
Helixi141 – 15010
Beta strandi162 – 1643
Helixi171 – 1766
Beta strandi177 – 1793
Turni193 – 1964
Helixi198 – 21316
Helixi227 – 24721
Helixi250 – 26213
Helixi273 – 28311
Beta strandi287 – 2904
Helixi297 – 3048
Turni305 – 3073
Beta strandi308 – 3114
Helixi329 – 3357
Helixi337 – 3404
Helixi343 – 35614
Helixi361 – 37212
Turni373 – 3775
Beta strandi379 – 3824
Beta strandi385 – 3884
Helixi391 – 3944
Helixi397 – 4037
Beta strandi405 – 4128
Helixi415 – 4195
Beta strandi425 – 4273
Beta strandi430 – 4367
Helixi438 – 45013
Beta strandi457 – 4615
Turni462 – 4654
Beta strandi466 – 4705
Helixi473 – 49624

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TOHX-ray2.30A156-498[»]
2MDANMR-A/B65-159[»]
2TOHX-ray2.30A156-498[»]
DisProtiDP00094.
ProteinModelPortaliP04177.
SMRiP04177. Positions 160-498.

Miscellaneous databases

EvolutionaryTraceiP04177.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 599Poly-Ala

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP04177.
KOiK00501.
OMAiELDKCHH.
OrthoDBiEOG7KM5T1.
PhylomeDBiP04177.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01269. Tyr_3_monoox. 1 hit.
PROSITEiPS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04177-1 [UniParc]FASTAAdd to Basket

« Hide

MPTPSAPSPQ PKGFRRAVSE QDAKQAEAVT SPRFIGRRQS LIEDARKERE    50
AAAAAAAAAV ASSEPGNPLE AVVFEERDGN AVLNLLFSLR GTKPSSLSRA 100
VKVFETFEAK IHHLETRPAQ RPLAGSPHLE YFVRFEVPSG DLAALLSSVR 150
RVSDDVRSAR EDKVPWFPRK VSELDKCHHL VTKFDPDLDL DHPGFSDQVY 200
RQRRKLIAEI AFQYKHGEPI PHVEYTAEEI ATWKEVYVTL KGLYATHACR 250
EHLEGFQLLE RYCGYREDSI PQLEDVSRFL KERTGFQLRP VAGLLSARDF 300
LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ 350
DIGLASLGAS DEEIEKLSTV YWFTVEFGLC KQNGELKAYG AGLLSSYGEL 400
LHSLSEEPEV RAFDPDTAAV QPYQDQTYQP VYFVSESFND AKDKLRNYAS 450
RIQRPFSVKF DPYTLAIDVL DSPHTIQRSL EGVQDELHTL AHALSAIS 498
Length:498
Mass (Da):55,966
Last modified:January 23, 2007 - v3
Checksum:i17F7E003D29218C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10244 mRNA. Translation: AAA42257.1.
L22651 mRNA. Translation: AAA42258.1.
PIRiA00510. WHRTY.
RefSeqiNP_036872.1. NM_012740.3.
UniGeneiRn.11082.

Genome annotation databases

EnsembliENSRNOT00000027682; ENSRNOP00000027682; ENSRNOG00000020410.
GeneIDi25085.
KEGGirno:25085.
UCSCiRGD:3853. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M10244 mRNA. Translation: AAA42257.1 .
L22651 mRNA. Translation: AAA42258.1 .
PIRi A00510. WHRTY.
RefSeqi NP_036872.1. NM_012740.3.
UniGenei Rn.11082.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TOH X-ray 2.30 A 156-498 [» ]
2MDA NMR - A/B 65-159 [» ]
2TOH X-ray 2.30 A 156-498 [» ]
DisProti DP00094.
ProteinModelPortali P04177.
SMRi P04177. Positions 160-498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247158. 1 interaction.
IntActi P04177. 4 interactions.
MINTi MINT-4585867.

Chemistry

BindingDBi P04177.
ChEMBLi CHEMBL2462.

PTM databases

PhosphoSitei P04177.

Proteomic databases

PaxDbi P04177.
PRIDEi P04177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000027682 ; ENSRNOP00000027682 ; ENSRNOG00000020410 .
GeneIDi 25085.
KEGGi rno:25085.
UCSCi RGD:3853. rat.

Organism-specific databases

CTDi 7054.
RGDi 3853. Th.

Phylogenomic databases

eggNOGi COG3186.
GeneTreei ENSGT00390000010268.
HOGENOMi HOG000233373.
HOVERGENi HBG006841.
InParanoidi P04177.
KOi K00501.
OMAi ELDKCHH.
OrthoDBi EOG7KM5T1.
PhylomeDBi P04177.
TreeFami TF313327.

Enzyme and pathway databases

UniPathwayi UPA00747 ; UER00733 .
SABIO-RK P04177.

Miscellaneous databases

EvolutionaryTracei P04177.
NextBioi 605346.
PROi P04177.

Gene expression databases

Genevestigatori P04177.

Family and domain databases

Gene3Di 1.10.800.10. 1 hit.
InterProi IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005962. Tyr_3_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
IPR021164. Tyrosine_hydroxylase_CS.
[Graphical view ]
PANTHERi PTHR11473. PTHR11473. 1 hit.
Pfami PF00351. Biopterin_H. 1 hit.
PF12549. TOH_N. 2 hits.
[Graphical view ]
PIRSFi PIRSF000336. TH. 1 hit.
PRINTSi PR00372. FYWHYDRXLASE.
SUPFAMi SSF56534. SSF56534. 1 hit.
TIGRFAMsi TIGR01269. Tyr_3_monoox. 1 hit.
PROSITEi PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Anton X.X., Manaster J.S., Kordower X.X., Markham X.X., Bredesen D.E.
    Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Bienvenut W.V., von Kriegsheim A.F., Kolch W.
    Submitted (AUG-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-12; 284-298 AND 452-459, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Pheochromocytoma.
  4. "Tyrosine hydroxylase in rat brain dopaminergic nerve terminals. Multiple-site phosphorylation in vivo and in synaptosomes."
    Haycock J.W., Haycock D.A.
    J. Biol. Chem. 266:5650-5657(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-19; SER-31 AND SER-40.
  5. "Phosphorylation of Ser(19) alters the conformation of tyrosine hydroxylase to increase the rate of phosphorylation of Ser(40)."
    Bevilaqua L.R., Graham M.E., Dunkley P.R., von Nagy-Felsobuki E.I., Dickson P.W.
    J. Biol. Chem. 276:40411-40416(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-19 AND SER-40, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Crystal structure of tyrosine hydroxylase at 2.3 A and its implications for inherited neurodegenerative diseases."
    Goodwill K.E., Sabatier C., Marks C., Raag R., Fitzpatrick P.F., Stevens R.C.
    Nat. Struct. Biol. 4:578-585(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 164-498.
  7. "Crystal structure of tyrosine hydroxylase with bound cofactor analogue and iron at 2.3 A resolution: self-hydroxylation of Phe300 and the pterin-binding site."
    Goodwill K.E., Sabatier C., Stevens R.C.
    Biochemistry 37:13437-13445(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 160-498.

Entry informationi

Entry nameiTY3H_RAT
AccessioniPrimary (citable) accession number: P04177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi