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Reviewed, UniProtKB/Swiss-Prot P04175 (NCPR_PIG)

Last modified June 16, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADPH--cytochrome P450 reductase
      Short name=CPR
      Short name=P450R
    EC=1.6.2.4
Gene names
Name: POR
OrganismSus scrofa (Pig)
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

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Protein attributes

Sequence length678 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activity

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactor

FAD.

FMN.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

Sequence similarities

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 678677NADPH--cytochrome P450 reductase
PRO_0000167598

Regions

Domain80 – 224145Flavodoxin-like
Domain279 – 521243FAD-binding FR-type
Nucleotide binding170 – 20132FMN By similarity
Nucleotide binding314 – 32512FAD By similarity
Nucleotide binding451 – 46111FAD By similarity
Nucleotide binding529 – 54719NADP By similarity
Nucleotide binding624 – 64017NADP By similarity

Amino acid modifications

Modified residue21N-acetylglycine Ref.2
Modified residue5751Phosphotyrosine By similarity

Experimental info

Sequence conflict551S → T in AAA85368. Ref.2
Sequence conflict1641T → S in AAA85368. Ref.2
Sequence conflict1751N → D AA sequence Ref.3
Sequence conflict3401T → A AA sequence Ref.3
Sequence conflict3791N → D AA sequence Ref.3
Sequence conflict4011Q → E AA sequence Ref.3
Sequence conflict4471R → L in AAA85368. Ref.2
Sequence conflict5031N → D AA sequence Ref.3
Sequence conflict5091V → L AA sequence Ref.2
Sequence conflict5091V → L AA sequence Ref.3
Sequence conflict6751D → N AA sequence Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P04175-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 206079E13CA6E0A8

FASTA67876,833
        10         20         30         40         50         60 
MGDSNVDTGT TTSEMVAEEV SLFSATDMVL FSLIVGLLTY WFIFRKKKDE VPEFSKIETT 

        70         80         90        100        110        120 
TSSVKDSSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM AADPEEYDLS 

       130        140        150        160        170        180 
DLSSLPEIEN ALAVFCMATY GEGDPTDNAQ DFYDWLQEAD VDLTGVKYAV FGLGNKTYEH 

       190        200        210        220        230        240 
FNAMGKYVDK RLEQLGAQRI FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES 

       250        260        270        280        290        300 
SIRQYELVVH TDMDTAVVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL 

       310        320        330        340        350        360 
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGT DLDIVMSLNN LDEESNKRHP 

       370        380        390        400        410        420 
FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE QLRKMASSSG EGKELYLSWV 

       430        440        450        460        470        480 
VEARRHILAI LQDYPSLRPP IDHLCERLPR LQARYYSIAS SSKVHPNSVH ICAVVVEYET 

       490        500        510        520        530        540 
KSGRVNKGVA TSWLRAKEPA GENGRRALVP MFVRKSQFRL PFKATTPVIM VGPGTGVAPF 

       550        560        570        580        590        600 
IGFIQERAWL QEQGKEVGET LLYYGCRRSD EDYLYREELA QFHAKGALTR LSVAFSREQP 

       610        620        630        640        650        660 
QKVYVQHLLK RDKEHLWKLI HDGGAHIYIC GDARNMARDV QNTFCDIVAE QGPMEHAQAV 

       670 
DYVKKLMTKG RYSLDVWS

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References

[1]Amborn J., Preiss B., Stender B., Viale M., Repp R.Z., Lampert F., Kroger M., Lumper L.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete amino acid sequence of NADPH-cytochrome P-450 reductase from porcine hepatic microsomes."
Haniu M., Iyanagi T., Miller P., Lee T.D., Shively J.E.
Biochemistry 25:7906-7911(1986) [PubMed: 3099837] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-678, ACETYLATION AT GLY-2.
[3]"Complete structure of the hydrophilic domain in the porcine NADPH-cytochrome P-450 reductase."
Vogel F., Lumper L.
Biochem. J. 236:871-878(1986) [PubMed: 3098240] [Abstract]
Cited for: PROTEIN SEQUENCE OF 57-678.

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Cross-references

Sequence databases

L33893 mRNA. Translation: AAA85368.1.
PIRRDPGO4. A25584.
RefSeqNP_001123431.1.
UniGeneSsc.50299

3D structure databases

HSSPHSSP built from PDB template 1B1C based on UniProtKB P16435.
SMRP04175. Positions 64-678.
ModBaseSearch...

Genome annotation databases

GeneID100170114.
KEGGssc:100170114.

Phylogenomic databases

HOVERGENP04175.

Enzyme and pathway databases

BRENDA1.6.2.4. 249.

Family and domain databases

InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR015702. NADPH_Cyt_P450_Rdtase.
IPR001433. OxRdtase_FAD/NAD_bd.
[Graphical view]
PANTHERPTHR19384:SF17. NADPH_Cyt_Red. 1 hit.
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNCPR_PIG
AccessionPrimary (citable) accession number: P04175
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents