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Protein

NADPH--cytochrome P450 reductase

Gene

POR

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208FMNUniRule annotation1
Binding sitei298NADPUniRule annotation1
Binding sitei424FADUniRule annotation1
Binding sitei478FADUniRule annotation1
Binding sitei535NADPUniRule annotation1
Binding sitei639NADPUniRule annotation1
Binding sitei677FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 91FMNUniRule annotation6
Nucleotide bindingi138 – 141FMNUniRule annotation4
Nucleotide bindingi173 – 182FMNUniRule annotation10
Nucleotide bindingi454 – 457FADUniRule annotation4
Nucleotide bindingi472 – 474FADUniRule annotation3
Nucleotide bindingi488 – 491FADUniRule annotation4
Nucleotide bindingi596 – 597NADPUniRule annotation2
Nucleotide bindingi602 – 606NADPUniRule annotation5

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PORUniRule annotation
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 22LumenalUniRule annotationAdd BLAST21
Transmembranei23 – 43HelicalUniRule annotationAdd BLAST21
Topological domaini44 – 678CytoplasmicUniRule annotationAdd BLAST635

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001675982 – 678NADPH--cytochrome P450 reductaseAdd BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine1 Publication1
Modified residuei63PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PeptideAtlasiP04175.
PRIDEiP04175.

Structurei

3D structure databases

ProteinModelPortaliP04175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 224Flavodoxin-likeUniRule annotationAdd BLAST145
Domaini279 – 521FAD-binding FR-typeUniRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000432.
InParanoidiP04175.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDSNVDTGT TTSEMVAEEV SLFSATDMVL FSLIVGLLTY WFIFRKKKDE
60 70 80 90 100
VPEFSKIETT TSSVKDSSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK
110 120 130 140 150
DAHRYGMRGM AADPEEYDLS DLSSLPEIEN ALAVFCMATY GEGDPTDNAQ
160 170 180 190 200
DFYDWLQEAD VDLTGVKYAV FGLGNKTYEH FNAMGKYVDK RLEQLGAQRI
210 220 230 240 250
FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES SIRQYELVVH
260 270 280 290 300
TDMDTAVVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL
310 320 330 340 350
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGT DLDIVMSLNN
360 370 380 390 400
LDEESNKRHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE
410 420 430 440 450
QLRKMASSSG EGKELYLSWV VEARRHILAI LQDYPSLRPP IDHLCERLPR
460 470 480 490 500
LQARYYSIAS SSKVHPNSVH ICAVVVEYET KSGRVNKGVA TSWLRAKEPA
510 520 530 540 550
GENGRRALVP MFVRKSQFRL PFKATTPVIM VGPGTGVAPF IGFIQERAWL
560 570 580 590 600
QEQGKEVGET LLYYGCRRSD EDYLYREELA QFHAKGALTR LSVAFSREQP
610 620 630 640 650
QKVYVQHLLK RDKEHLWKLI HDGGAHIYIC GDARNMARDV QNTFCDIVAE
660 670
QGPMEHAQAV DYVKKLMTKG RYSLDVWS
Length:678
Mass (Da):76,833
Last modified:January 23, 2007 - v2
Checksum:i206079E13CA6E0A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55S → T in AAA85368 (Ref. 1) Curated1
Sequence conflicti164T → S in AAA85368 (Ref. 1) Curated1
Sequence conflicti175N → D AA sequence (PubMed:3098240).Curated1
Sequence conflicti340T → A AA sequence (PubMed:3098240).Curated1
Sequence conflicti379N → D AA sequence (PubMed:3098240).Curated1
Sequence conflicti401Q → E AA sequence (PubMed:3098240).Curated1
Sequence conflicti447R → L in AAA85368 (Ref. 1) Curated1
Sequence conflicti503N → D AA sequence (PubMed:3098240).Curated1
Sequence conflicti509V → L AA sequence (PubMed:3099837).Curated1
Sequence conflicti509V → L AA sequence (PubMed:3098240).Curated1
Sequence conflicti675D → N AA sequence (PubMed:3098240).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33893 mRNA. Translation: AAA85368.1.
PIRiA25584. RDPGO4.
RefSeqiNP_001123431.1. NM_001129959.1.
UniGeneiSsc.50299.

Genome annotation databases

GeneIDi100170114.
KEGGissc:100170114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33893 mRNA. Translation: AAA85368.1.
PIRiA25584. RDPGO4.
RefSeqiNP_001123431.1. NM_001129959.1.
UniGeneiSsc.50299.

3D structure databases

ProteinModelPortaliP04175.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PeptideAtlasiP04175.
PRIDEiP04175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100170114.
KEGGissc:100170114.

Organism-specific databases

CTDi5447.

Phylogenomic databases

HOVERGENiHBG000432.
InParanoidiP04175.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCPR_PIG
AccessioniPrimary (citable) accession number: P04175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.