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Protein

Cytochrome P450 2B2

Gene

Cyp2b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi436Iron (heme axial ligand)1

GO - Molecular functioni

  • arachidonic acid epoxygenase activity Source: GO_Central
  • aromatase activity Source: UniProtKB-EC
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • oxidoreductase activity Source: RGD
  • steroid hydroxylase activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • drug metabolic process Source: RGD
  • epoxygenase P450 pathway Source: GO_Central
  • nicotine metabolic process Source: RGD
  • response to calcium ion Source: RGD
  • response to metal ion Source: RGD
  • response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B2 (EC:1.14.14.1)
Alternative name(s):
CYPIIB2
Cytochrome P450 PB4
Cytochrome P450E
Gene namesi
Name:Cyp2b2
Synonyms:Cyp2b-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2467. Cyp2b2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

DrugBankiDB08834. Tauroursodeoxycholic acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516791 – 491Cytochrome P450 2B2Add BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128Phosphoserine; by PKA1 Publication1

Post-translational modificationi

Phosphorylation is accompanied by a decrease in enzyme activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP04167.
PeptideAtlasiP04167.
PRIDEiP04167.

PTM databases

iPTMnetiP04167.

Expressioni

Inductioni

By phenobarbital.

Interactioni

Protein-protein interaction databases

MINTiMINT-4571939.
STRINGi10116.ENSRNOP00000045196.

Structurei

3D structure databases

ProteinModelPortaliP04167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP04167.
KOiK07412.
PhylomeDBiP04167.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04167-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPSILLLLA LLVGFLLLLV RGHPKSRGNF PPGPRPLPLL GNLLQLDRGG
60 70 80 90 100
LLNSFMQLRE KYGDVFTVHL GPRPVVMLCG TDTIKEALVG QAEDFSGRGT
110 120 130 140 150
IAVIEPIFKE YGVIFANGER WKALRRFSLA TMRDFGMGKR SVEERIQEEA
160 170 180 190 200
QCLVEELRKS QGAPLDPTFL FQCITANIIC SIVFGERFDY TDRQFLRLLE
210 220 230 240 250
LFYRTFSLLS SFSSQVFEFF SGFLKYFPGA HRQISKNLQE ILDYIGHIVE
260 270 280 290 300
KHRATLDPSA PRDFIDTYLL RMEKEKSNHH TEFHHENLMI SLLSLFFAGT
310 320 330 340 350
ETGSTTLRYG FLLMLKYPHV TEKVQKEIDQ VIGSHRPPSL DDRTKMPYTD
360 370 380 390 400
AVIHEIQRFA DLAPIGLPHR VTKDTMFRGY LLPKNTEVYP ILSSALHDPQ
410 420 430 440 450
YFDHPDTFNP EHFLDADGTL KKSEAFMPFS TGKRICLGEG IARNELFLFF
460 470 480 490
TTILQNFSVS SHLAPKDIDL TPKESGIAKI PPTYQICFSA R
Length:491
Mass (Da):55,933
Last modified:July 15, 1998 - v2
Checksum:i00CB6B937FDD44BC
GO
Isoform 2 (identifier: P04167-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-274: K → KVSPAWMRE

Note: No experimental confirmation available.
Show »
Length:499
Mass (Da):56,890
Checksum:iA1359447DBDA2983
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114I → F in AAA41004 (PubMed:2323573).Curated1
Sequence conflicti292L → P AA sequence (PubMed:3877725).Curated1
Sequence conflicti321T → A AA sequence (PubMed:3877725).Curated1
Sequence conflicti321T → A in AAA41029 (PubMed:6688421).Curated1
Sequence conflicti322E → V in AAA41056 (PubMed:6306654).Curated1
Sequence conflicti359 – 360FA → AS no nucleotide entry (PubMed:2539047).Curated2
Sequence conflicti438G → D in AAA41029 (PubMed:6688421).Curated1
Sequence conflicti444N → K in AAA41026 (PubMed:6689485).Curated1
Sequence conflicti473K → M in AAA41056 (PubMed:6306654).Curated1
Sequence conflicti476G → D AA sequence (PubMed:3877725).Curated1
Sequence conflicti476G → D in AAA41029 (PubMed:6688421).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011939274K → KVSPAWMRE in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00728
, J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA. Translation: AAA41056.1.
M34452 mRNA. Translation: AAA41004.1.
K01721 mRNA. Translation: AAA41026.1.
K00996 mRNA. Translation: AAA41029.1.
M13234 Genomic DNA. Translation: AAA41057.1.
K01626 mRNA. Translation: AAA41037.1.
D00250 Genomic DNA. Translation: BAA00181.1.
PIRiA21162. O4RTP2.
A60822.
RefSeqiNP_001185605.1. NM_001198676.1.
UniGeneiRn.228579.
Rn.91353.

Genome annotation databases

GeneIDi361523.
KEGGirno:361523.
UCSCiRGD:2467. rat. [P04167-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00728
, J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA. Translation: AAA41056.1.
M34452 mRNA. Translation: AAA41004.1.
K01721 mRNA. Translation: AAA41026.1.
K00996 mRNA. Translation: AAA41029.1.
M13234 Genomic DNA. Translation: AAA41057.1.
K01626 mRNA. Translation: AAA41037.1.
D00250 Genomic DNA. Translation: BAA00181.1.
PIRiA21162. O4RTP2.
A60822.
RefSeqiNP_001185605.1. NM_001198676.1.
UniGeneiRn.228579.
Rn.91353.

3D structure databases

ProteinModelPortaliP04167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4571939.
STRINGi10116.ENSRNOP00000045196.

Chemistry databases

DrugBankiDB08834. Tauroursodeoxycholic acid.

PTM databases

iPTMnetiP04167.

Proteomic databases

PaxDbiP04167.
PeptideAtlasiP04167.
PRIDEiP04167.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi361523.
KEGGirno:361523.
UCSCiRGD:2467. rat. [P04167-1]

Organism-specific databases

CTDi361523.
RGDi2467. Cyp2b2.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP04167.
KOiK07412.
PhylomeDBiP04167.

Miscellaneous databases

PROiP04167.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP2B2_RAT
AccessioniPrimary (citable) accession number: P04167
Secondary accession number(s): Q64579, Q64582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: July 15, 1998
Last modified: October 5, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.