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P04167 (CP2B2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2B2

EC=1.14.14.1
Alternative name(s):
CYPIIB2
Cytochrome P450 PB4
Cytochrome P450E
Gene names
Name:Cyp2b2
Synonyms:Cyp2b-2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By phenobarbital.

Post-translational modification

Phosphorylation is accompanied by a decrease in enzyme activity.

Sequence similarities

Belongs to the cytochrome P450 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P04167-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P04167-2)

The sequence of this isoform differs from the canonical sequence as follows:
     274-274: K → KVSPAWMRE
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Cytochrome P450 2B2
PRO_0000051679

Sites

Metal binding4361Iron (heme axial ligand)

Amino acid modifications

Modified residue1281Phosphoserine; by PKA Ref.12

Natural variations

Alternative sequence2741K → KVSPAWMRE in isoform 2.
VSP_011939

Experimental info

Sequence conflict1141I → F in AAA41004. Ref.3
Sequence conflict2921L → P AA sequence Ref.2
Sequence conflict3211T → A AA sequence Ref.2
Sequence conflict3211T → A in AAA41029. Ref.5
Sequence conflict3221E → V in AAA41056. Ref.1
Sequence conflict359 – 3602FA → AS no nucleotide entry Ref.8
Sequence conflict4381G → D in AAA41029. Ref.5
Sequence conflict4441N → K in AAA41026. Ref.4
Sequence conflict4731K → M in AAA41056. Ref.1
Sequence conflict4761G → D AA sequence Ref.2
Sequence conflict4761G → D in AAA41029. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 00CB6B937FDD44BC

FASTA49155,933
        10         20         30         40         50         60 
MEPSILLLLA LLVGFLLLLV RGHPKSRGNF PPGPRPLPLL GNLLQLDRGG LLNSFMQLRE 

        70         80         90        100        110        120 
KYGDVFTVHL GPRPVVMLCG TDTIKEALVG QAEDFSGRGT IAVIEPIFKE YGVIFANGER 

       130        140        150        160        170        180 
WKALRRFSLA TMRDFGMGKR SVEERIQEEA QCLVEELRKS QGAPLDPTFL FQCITANIIC 

       190        200        210        220        230        240 
SIVFGERFDY TDRQFLRLLE LFYRTFSLLS SFSSQVFEFF SGFLKYFPGA HRQISKNLQE 

       250        260        270        280        290        300 
ILDYIGHIVE KHRATLDPSA PRDFIDTYLL RMEKEKSNHH TEFHHENLMI SLLSLFFAGT 

       310        320        330        340        350        360 
ETGSTTLRYG FLLMLKYPHV TEKVQKEIDQ VIGSHRPPSL DDRTKMPYTD AVIHEIQRFA 

       370        380        390        400        410        420 
DLAPIGLPHR VTKDTMFRGY LLPKNTEVYP ILSSALHDPQ YFDHPDTFNP EHFLDADGTL 

       430        440        450        460        470        480 
KKSEAFMPFS TGKRICLGEG IARNELFLFF TTILQNFSVS SHLAPKDIDL TPKESGIAKI 

       490 
PPTYQICFSA R 

« Hide

Isoform 2 [UniParc].

Checksum: A1359447DBDA2983
Show »

FASTA49956,890

References

[1]"Gene structure of a phenobarbital-inducible cytochrome P-450 in rat liver."
Mizukami Y., Sogawa K., Suwa Y., Muramatsu M., Fujii-Kuriyama Y.
Proc. Natl. Acad. Sci. U.S.A. 80:3958-3962(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]"The structure of phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4. Production and characterization of site-specific antibodies."
Frey A.B., Waxman D.J., Kreibich G.
J. Biol. Chem. 260:15253-15265(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE (ISOFORM 1).
[3]"Alternative splicing of mRNA encoding rat liver cytochrome P450e (P450IIB2)."
Lacroix D., Desrochers M., Lambert M., Anderson A.
Gene 86:201-207(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-491 (ISOFORM 2).
[4]"Cloning and sequence analysis of a rat liver cDNA coding for a phenobarbital-inducible microheterogenous cytochrome P-450 variant: regulation of its messenger level by xenobiotics."
Phillips I.R., Shephard E.A., Ashworth A., Rabin B.R.
Gene 26:41-52(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 168-491 (ISOFORM 1).
[5]"Cloned cytochrome P-450 cDNA. Nucleotide sequence and homology to multiple phenobarbital-induced mRNA species."
Kumar A., Raphael C., Adesnik M.
J. Biol. Chem. 258:11280-11284(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 281-491.
[6]Erratum
Kumar A., Raphael C., Adesnik M.
J. Biol. Chem. 259:6039-6039(1984)
[7]"Gene conversion in a cytochrome P-450 gene family."
Atchison M.L., Adesnik M.
Proc. Natl. Acad. Sci. U.S.A. 83:2300-2304(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 323-431.
[8]"Antibodies targeted against hypervariable and constant regions of cytochromes P450IIB1 and P450IIB2."
Oesch F., Waxman D.J., Morrissey J.J., Honscha W., Kissel W., Friedberg T.
Arch. Biochem. Biophys. 270:23-32(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-380 AND 402-423.
[9]"Segmental homologies in the coding and 3' non-coding sequences of rat liver cytochrome P-450e and P-450b cDNAs and cytochrome P-450e-like genes."
Affolter M., Anderson A.
Biochem. Biophys. Res. Commun. 118:655-662(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 385-491.
[10]"A mutant rat strain deficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes."
Hashimoto T., Matsumoto T., Nishizawa M., Kawabata S., Morohashi K., Handa S., Omura T.
J. Biochem. 103:487-492(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
[11]"Effect of nutritional imbalances on cytochrome P-450 isozymes in rat liver."
Amelizad Z., Narbonne J.F., Wolf C.R., Robertson L.W., Oesch F.
Biochem. Pharmacol. 37:3245-3249(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-20.
Tissue: Liver.
[12]"Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450."
Pyerin W., Taniguchi H.
EMBO J. 8:3003-3010(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-128.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J00728 expand/collapse EMBL AC list , J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA. Translation: AAA41056.1.
M34452 mRNA. Translation: AAA41004.1.
K01721 mRNA. Translation: AAA41026.1.
K00996 mRNA. Translation: AAA41029.1.
M13234 Genomic DNA. Translation: AAA41057.1.
K01626 mRNA. Translation: AAA41037.1.
D00250 Genomic DNA. Translation: BAA00181.1.
PIRO4RTP2. A21162.
A60822.
RefSeqNP_001185605.1. NM_001198676.1.
UniGeneRn.228579.
Rn.91353.

3D structure databases

ProteinModelPortalP04167.
SMRP04167. Positions 28-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4571939.

Proteomic databases

PaxDbP04167.
PRIDEP04167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID361523.
KEGGrno:361523.
UCSCRGD:2467. rat. [P04167-1]

Organism-specific databases

CTD361523.
RGD2467. Cyp2b2.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036992.
HOVERGENHBG015789.
InParanoidP04167.
KOK07412.
PhylomeDBP04167.

Gene expression databases

GenevestigatorP04167.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio676604.

Entry information

Entry nameCP2B2_RAT
AccessionPrimary (citable) accession number: P04167
Secondary accession number(s): Q64579, Q64582
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: July 15, 1998
Last modified: April 16, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families