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Protein

Cytochrome P450 2B2

Gene

Cyp2b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: GO_Central
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. oxidoreductase activity Source: RGD
  6. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  7. oxygen binding Source: GO_Central
  8. steroid hydroxylase activity Source: RGD

GO - Biological processi

  1. aging Source: RGD
  2. drug metabolic process Source: RGD
  3. epoxygenase P450 pathway Source: GO_Central
  4. exogenous drug catabolic process Source: GO_Central
  5. nicotine metabolic process Source: RGD
  6. oxidation-reduction process Source: GO_Central
  7. response to calcium ion Source: RGD
  8. response to metal ion Source: RGD
  9. response to organic cyclic compound Source: RGD
  10. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B2 (EC:1.14.14.1)
Alternative name(s):
CYPIIB2
Cytochrome P450 PB4
Cytochrome P450E
Gene namesi
Name:Cyp2b2
Synonyms:Cyp2b-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2467. Cyp2b2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Cytochrome P450 2B2PRO_0000051679Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine; by PKA1 Publication

Post-translational modificationi

Phosphorylation is accompanied by a decrease in enzyme activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP04167.
PRIDEiP04167.

Expressioni

Inductioni

By phenobarbital.

Gene expression databases

GenevestigatoriP04167.

Interactioni

Protein-protein interaction databases

MINTiMINT-4571939.

Structurei

3D structure databases

ProteinModelPortaliP04167.
SMRiP04167. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP04167.
KOiK07412.
PhylomeDBiP04167.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04167-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPSILLLLA LLVGFLLLLV RGHPKSRGNF PPGPRPLPLL GNLLQLDRGG
60 70 80 90 100
LLNSFMQLRE KYGDVFTVHL GPRPVVMLCG TDTIKEALVG QAEDFSGRGT
110 120 130 140 150
IAVIEPIFKE YGVIFANGER WKALRRFSLA TMRDFGMGKR SVEERIQEEA
160 170 180 190 200
QCLVEELRKS QGAPLDPTFL FQCITANIIC SIVFGERFDY TDRQFLRLLE
210 220 230 240 250
LFYRTFSLLS SFSSQVFEFF SGFLKYFPGA HRQISKNLQE ILDYIGHIVE
260 270 280 290 300
KHRATLDPSA PRDFIDTYLL RMEKEKSNHH TEFHHENLMI SLLSLFFAGT
310 320 330 340 350
ETGSTTLRYG FLLMLKYPHV TEKVQKEIDQ VIGSHRPPSL DDRTKMPYTD
360 370 380 390 400
AVIHEIQRFA DLAPIGLPHR VTKDTMFRGY LLPKNTEVYP ILSSALHDPQ
410 420 430 440 450
YFDHPDTFNP EHFLDADGTL KKSEAFMPFS TGKRICLGEG IARNELFLFF
460 470 480 490
TTILQNFSVS SHLAPKDIDL TPKESGIAKI PPTYQICFSA R
Length:491
Mass (Da):55,933
Last modified:July 15, 1998 - v2
Checksum:i00CB6B937FDD44BC
GO
Isoform 2 (identifier: P04167-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-274: K → KVSPAWMRE

Note: No experimental confirmation available.

Show »
Length:499
Mass (Da):56,890
Checksum:iA1359447DBDA2983
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141I → F in AAA41004 (PubMed:2323573).Curated
Sequence conflicti292 – 2921L → P AA sequence (PubMed:3877725).Curated
Sequence conflicti321 – 3211T → A AA sequence (PubMed:3877725).Curated
Sequence conflicti321 – 3211T → A in AAA41029 (PubMed:6688421).Curated
Sequence conflicti322 – 3221E → V in AAA41056 (PubMed:6306654).Curated
Sequence conflicti359 – 3602FA → AS no nucleotide entry (PubMed:2539047).Curated
Sequence conflicti438 – 4381G → D in AAA41029 (PubMed:6688421).Curated
Sequence conflicti444 – 4441N → K in AAA41026 (PubMed:6689485).Curated
Sequence conflicti473 – 4731K → M in AAA41056 (PubMed:6306654).Curated
Sequence conflicti476 – 4761G → D AA sequence (PubMed:3877725).Curated
Sequence conflicti476 – 4761G → D in AAA41029 (PubMed:6688421).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei274 – 2741K → KVSPAWMRE in isoform 2. 1 PublicationVSP_011939

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00728
, J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA. Translation: AAA41056.1.
M34452 mRNA. Translation: AAA41004.1.
K01721 mRNA. Translation: AAA41026.1.
K00996 mRNA. Translation: AAA41029.1.
M13234 Genomic DNA. Translation: AAA41057.1.
K01626 mRNA. Translation: AAA41037.1.
D00250 Genomic DNA. Translation: BAA00181.1.
PIRiA21162. O4RTP2.
A60822.
RefSeqiNP_001185605.1. NM_001198676.1.
UniGeneiRn.228579.
Rn.91353.

Genome annotation databases

GeneIDi361523.
KEGGirno:361523.
UCSCiRGD:2467. rat. [P04167-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00728
, J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA. Translation: AAA41056.1.
M34452 mRNA. Translation: AAA41004.1.
K01721 mRNA. Translation: AAA41026.1.
K00996 mRNA. Translation: AAA41029.1.
M13234 Genomic DNA. Translation: AAA41057.1.
K01626 mRNA. Translation: AAA41037.1.
D00250 Genomic DNA. Translation: BAA00181.1.
PIRiA21162. O4RTP2.
A60822.
RefSeqiNP_001185605.1. NM_001198676.1.
UniGeneiRn.228579.
Rn.91353.

3D structure databases

ProteinModelPortaliP04167.
SMRiP04167. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4571939.

Chemistry

DrugBankiDB08834. Tauroursodeoxycholic acid.

Proteomic databases

PaxDbiP04167.
PRIDEiP04167.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi361523.
KEGGirno:361523.
UCSCiRGD:2467. rat. [P04167-1]

Organism-specific databases

CTDi361523.
RGDi2467. Cyp2b2.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP04167.
KOiK07412.
PhylomeDBiP04167.

Miscellaneous databases

NextBioi676604.

Gene expression databases

GenevestigatoriP04167.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Gene structure of a phenobarbital-inducible cytochrome P-450 in rat liver."
    Mizukami Y., Sogawa K., Suwa Y., Muramatsu M., Fujii-Kuriyama Y.
    Proc. Natl. Acad. Sci. U.S.A. 80:3958-3962(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  2. "The structure of phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4. Production and characterization of site-specific antibodies."
    Frey A.B., Waxman D.J., Kreibich G.
    J. Biol. Chem. 260:15253-15265(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE (ISOFORM 1).
  3. "Alternative splicing of mRNA encoding rat liver cytochrome P450e (P450IIB2)."
    Lacroix D., Desrochers M., Lambert M., Anderson A.
    Gene 86:201-207(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-491 (ISOFORM 2).
  4. "Cloning and sequence analysis of a rat liver cDNA coding for a phenobarbital-inducible microheterogenous cytochrome P-450 variant: regulation of its messenger level by xenobiotics."
    Phillips I.R., Shephard E.A., Ashworth A., Rabin B.R.
    Gene 26:41-52(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 168-491 (ISOFORM 1).
  5. "Cloned cytochrome P-450 cDNA. Nucleotide sequence and homology to multiple phenobarbital-induced mRNA species."
    Kumar A., Raphael C., Adesnik M.
    J. Biol. Chem. 258:11280-11284(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 281-491.
  6. Erratum
    Kumar A., Raphael C., Adesnik M.
    J. Biol. Chem. 259:6039-6039(1984)
  7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 323-431.
  8. "Antibodies targeted against hypervariable and constant regions of cytochromes P450IIB1 and P450IIB2."
    Oesch F., Waxman D.J., Morrissey J.J., Honscha W., Kissel W., Friedberg T.
    Arch. Biochem. Biophys. 270:23-32(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-380 AND 402-423.
  9. "Segmental homologies in the coding and 3' non-coding sequences of rat liver cytochrome P-450e and P-450b cDNAs and cytochrome P-450e-like genes."
    Affolter M., Anderson A.
    Biochem. Biophys. Res. Commun. 118:655-662(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 385-491.
  10. "A mutant rat strain deficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes."
    Hashimoto T., Matsumoto T., Nishizawa M., Kawabata S., Morohashi K., Handa S., Omura T.
    J. Biochem. 103:487-492(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
  11. "Effect of nutritional imbalances on cytochrome P-450 isozymes in rat liver."
    Amelizad Z., Narbonne J.F., Wolf C.R., Robertson L.W., Oesch F.
    Biochem. Pharmacol. 37:3245-3249(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20.
    Tissue: Liver.
  12. "Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450."
    Pyerin W., Taniguchi H.
    EMBO J. 8:3003-3010(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-128.

Entry informationi

Entry nameiCP2B2_RAT
AccessioniPrimary (citable) accession number: P04167
Secondary accession number(s): Q64579, Q64582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: July 15, 1998
Last modified: February 4, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.