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P04167

- CP2B2_RAT

UniProt

P04167 - CP2B2_RAT

Protein

Cytochrome P450 2B2

Gene

Cyp2b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi436 – 4361Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. oxidoreductase activity Source: RGD
    5. steroid hydroxylase activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. drug metabolic process Source: RGD
    3. nicotine metabolic process Source: RGD
    4. response to calcium ion Source: RGD
    5. response to metal ion Source: RGD
    6. response to organic cyclic compound Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2B2 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIB2
    Cytochrome P450 PB4
    Cytochrome P450E
    Gene namesi
    Name:Cyp2b2
    Synonyms:Cyp2b-2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2467. Cyp2b2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Cytochrome P450 2B2PRO_0000051679Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281Phosphoserine; by PKA1 Publication

    Post-translational modificationi

    Phosphorylation is accompanied by a decrease in enzyme activity.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP04167.
    PRIDEiP04167.

    Expressioni

    Inductioni

    By phenobarbital.

    Gene expression databases

    GenevestigatoriP04167.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4571939.

    Structurei

    3D structure databases

    ProteinModelPortaliP04167.
    SMRiP04167. Positions 28-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiP04167.
    KOiK07412.
    PhylomeDBiP04167.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P04167-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPSILLLLA LLVGFLLLLV RGHPKSRGNF PPGPRPLPLL GNLLQLDRGG    50
    LLNSFMQLRE KYGDVFTVHL GPRPVVMLCG TDTIKEALVG QAEDFSGRGT 100
    IAVIEPIFKE YGVIFANGER WKALRRFSLA TMRDFGMGKR SVEERIQEEA 150
    QCLVEELRKS QGAPLDPTFL FQCITANIIC SIVFGERFDY TDRQFLRLLE 200
    LFYRTFSLLS SFSSQVFEFF SGFLKYFPGA HRQISKNLQE ILDYIGHIVE 250
    KHRATLDPSA PRDFIDTYLL RMEKEKSNHH TEFHHENLMI SLLSLFFAGT 300
    ETGSTTLRYG FLLMLKYPHV TEKVQKEIDQ VIGSHRPPSL DDRTKMPYTD 350
    AVIHEIQRFA DLAPIGLPHR VTKDTMFRGY LLPKNTEVYP ILSSALHDPQ 400
    YFDHPDTFNP EHFLDADGTL KKSEAFMPFS TGKRICLGEG IARNELFLFF 450
    TTILQNFSVS SHLAPKDIDL TPKESGIAKI PPTYQICFSA R 491
    Length:491
    Mass (Da):55,933
    Last modified:July 15, 1998 - v2
    Checksum:i00CB6B937FDD44BC
    GO
    Isoform 2 (identifier: P04167-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         274-274: K → KVSPAWMRE

    Note: No experimental confirmation available.

    Show »
    Length:499
    Mass (Da):56,890
    Checksum:iA1359447DBDA2983
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141I → F in AAA41004. (PubMed:2323573)Curated
    Sequence conflicti292 – 2921L → P AA sequence (PubMed:3877725)Curated
    Sequence conflicti321 – 3211T → A AA sequence (PubMed:3877725)Curated
    Sequence conflicti321 – 3211T → A in AAA41029. (PubMed:6688421)Curated
    Sequence conflicti322 – 3221E → V in AAA41056. (PubMed:6306654)Curated
    Sequence conflicti359 – 3602FA → AS no nucleotide entry (PubMed:2539047)Curated
    Sequence conflicti438 – 4381G → D in AAA41029. (PubMed:6688421)Curated
    Sequence conflicti444 – 4441N → K in AAA41026. (PubMed:6689485)Curated
    Sequence conflicti473 – 4731K → M in AAA41056. (PubMed:6306654)Curated
    Sequence conflicti476 – 4761G → D AA sequence (PubMed:3877725)Curated
    Sequence conflicti476 – 4761G → D in AAA41029. (PubMed:6688421)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei274 – 2741K → KVSPAWMRE in isoform 2. 1 PublicationVSP_011939

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00728
    , J00720, J00721, J00722, J00723, J00724, J00725, J00726 Genomic DNA. Translation: AAA41056.1.
    M34452 mRNA. Translation: AAA41004.1.
    K01721 mRNA. Translation: AAA41026.1.
    K00996 mRNA. Translation: AAA41029.1.
    M13234 Genomic DNA. Translation: AAA41057.1.
    K01626 mRNA. Translation: AAA41037.1.
    D00250 Genomic DNA. Translation: BAA00181.1.
    PIRiA21162. O4RTP2.
    A60822.
    RefSeqiNP_001185605.1. NM_001198676.1.
    UniGeneiRn.228579.
    Rn.91353.

    Genome annotation databases

    GeneIDi361523.
    KEGGirno:361523.
    UCSCiRGD:2467. rat. [P04167-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00728
    , J00720 , J00721 , J00722 , J00723 , J00724 , J00725 , J00726 Genomic DNA. Translation: AAA41056.1 .
    M34452 mRNA. Translation: AAA41004.1 .
    K01721 mRNA. Translation: AAA41026.1 .
    K00996 mRNA. Translation: AAA41029.1 .
    M13234 Genomic DNA. Translation: AAA41057.1 .
    K01626 mRNA. Translation: AAA41037.1 .
    D00250 Genomic DNA. Translation: BAA00181.1 .
    PIRi A21162. O4RTP2.
    A60822.
    RefSeqi NP_001185605.1. NM_001198676.1.
    UniGenei Rn.228579.
    Rn.91353.

    3D structure databases

    ProteinModelPortali P04167.
    SMRi P04167. Positions 28-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4571939.

    Proteomic databases

    PaxDbi P04167.
    PRIDEi P04167.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 361523.
    KEGGi rno:361523.
    UCSCi RGD:2467. rat. [P04167-1 ]

    Organism-specific databases

    CTDi 361523.
    RGDi 2467. Cyp2b2.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi P04167.
    KOi K07412.
    PhylomeDBi P04167.

    Miscellaneous databases

    NextBioi 676604.

    Gene expression databases

    Genevestigatori P04167.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure of a phenobarbital-inducible cytochrome P-450 in rat liver."
      Mizukami Y., Sogawa K., Suwa Y., Muramatsu M., Fujii-Kuriyama Y.
      Proc. Natl. Acad. Sci. U.S.A. 80:3958-3962(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    2. "The structure of phenobarbital-inducible rat liver cytochrome P-450 isoenzyme PB-4. Production and characterization of site-specific antibodies."
      Frey A.B., Waxman D.J., Kreibich G.
      J. Biol. Chem. 260:15253-15265(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE (ISOFORM 1).
    3. "Alternative splicing of mRNA encoding rat liver cytochrome P450e (P450IIB2)."
      Lacroix D., Desrochers M., Lambert M., Anderson A.
      Gene 86:201-207(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-491 (ISOFORM 2).
    4. "Cloning and sequence analysis of a rat liver cDNA coding for a phenobarbital-inducible microheterogenous cytochrome P-450 variant: regulation of its messenger level by xenobiotics."
      Phillips I.R., Shephard E.A., Ashworth A., Rabin B.R.
      Gene 26:41-52(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 168-491 (ISOFORM 1).
    5. "Cloned cytochrome P-450 cDNA. Nucleotide sequence and homology to multiple phenobarbital-induced mRNA species."
      Kumar A., Raphael C., Adesnik M.
      J. Biol. Chem. 258:11280-11284(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 281-491.
    6. Erratum
      Kumar A., Raphael C., Adesnik M.
      J. Biol. Chem. 259:6039-6039(1984)
    7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 323-431.
    8. "Antibodies targeted against hypervariable and constant regions of cytochromes P450IIB1 and P450IIB2."
      Oesch F., Waxman D.J., Morrissey J.J., Honscha W., Kissel W., Friedberg T.
      Arch. Biochem. Biophys. 270:23-32(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 329-380 AND 402-423.
    9. "Segmental homologies in the coding and 3' non-coding sequences of rat liver cytochrome P-450e and P-450b cDNAs and cytochrome P-450e-like genes."
      Affolter M., Anderson A.
      Biochem. Biophys. Res. Commun. 118:655-662(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 385-491.
    10. "A mutant rat strain deficient in induction of a phenobarbital-inducible form of cytochrome P-450 in liver microsomes."
      Hashimoto T., Matsumoto T., Nishizawa M., Kawabata S., Morohashi K., Handa S., Omura T.
      J. Biochem. 103:487-492(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
    11. "Effect of nutritional imbalances on cytochrome P-450 isozymes in rat liver."
      Amelizad Z., Narbonne J.F., Wolf C.R., Robertson L.W., Oesch F.
      Biochem. Pharmacol. 37:3245-3249(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20.
      Tissue: Liver.
    12. "Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450."
      Pyerin W., Taniguchi H.
      EMBO J. 8:3003-3010(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-128.

    Entry informationi

    Entry nameiCP2B2_RAT
    AccessioniPrimary (citable) accession number: P04167
    Secondary accession number(s): Q64579, Q64582
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 1987
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3