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Protein

Cytochrome b5 type B

Gene

Cyb5b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Iron (heme axial ligand)1
Metal bindingi79Iron (heme axial ligand)1

GO - Molecular functioni

  • electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity Source: RGD
  • enzyme activator activity Source: RGD
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5 type B
Alternative name(s):
Cytochrome b5 outer mitochondrial membrane isoform
Gene namesi
Name:Cyb5b
Synonyms:Cyb5m, Omb5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi621551. Cyb5b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei119 – 136HelicalSequence analysisAdd BLAST18

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial outer membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00000064772 – 111 Publication10
ChainiPRO_000000647812 – 146Cytochrome b5 type BAdd BLAST135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei30N6-acetyllysineBy similarity1
Modified residuei80PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04166.
PRIDEiP04166.

PTM databases

iPTMnetiP04166.
PhosphoSitePlusiP04166.
SwissPalmiP04166.

Expressioni

Gene expression databases

BgeeiENSRNOG00000011142.
GenevisibleiP04166. RN.

Interactioni

Subunit structurei

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2.By similarity

Protein-protein interaction databases

MINTiMINT-4996332.
STRINGi10116.ENSRNOP00000015006.

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 28Combined sources4
Beta strandi36 – 41Combined sources6
Beta strandi44 – 47Combined sources4
Helixi49 – 51Combined sources3
Turni52 – 54Combined sources3
Helixi60 – 63Combined sources4
Turni64 – 67Combined sources4
Helixi71 – 77Combined sources7
Helixi81 – 85Combined sources5
Helixi86 – 89Combined sources4
Beta strandi91 – 95Combined sources5
Helixi97 – 99Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AWPX-ray2.00A/B13-103[»]
1B5MX-ray2.70A19-102[»]
1EUEX-ray1.80A/B17-102[»]
1ICCX-ray2.00A/B/C/D17-103[»]
1LJ0X-ray2.00A/B/C/D12-103[»]
2I89X-ray2.10A/B/C/D12-103[»]
3MUSX-ray2.00A/B17-102[»]
4HILX-ray1.25A/B17-102[»]
ProteinModelPortaliP04166.
SMRiP04166.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04166.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 96Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP04166.
OMAiYYMAESK.
OrthoDBiEOG091G17G8.
PhylomeDBiP04166.
TreeFamiTF314537.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
3.10.120.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPEASGSG RNGQGSDPAV TYYRLEEVAK RNTAEETWMV IHGRVYDITR
60 70 80 90 100
FLSEHPGGEE VLLEQAGADA TESFEDVGHS PDAREMLKQY YIGDVHPNDL
110 120 130 140
KPKDGDKDPS KNNSCQSSWA YWIVPIVGAI LIGFLYRHFW ADSKSS
Length:146
Mass (Da):16,265
Last modified:January 11, 2001 - v2
Checksum:i1CA90DD3C81C412E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12N → D AA sequence (PubMed:6840088).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12517 mRNA. Translation: CAA73117.1.
BC072535 mRNA. Translation: AAH72535.1.
RefSeqiNP_085075.1. NM_030586.2.
UniGeneiRn.10249.

Genome annotation databases

EnsembliENSRNOT00000015006; ENSRNOP00000015006; ENSRNOG00000011142.
ENSRNOT00000086435; ENSRNOP00000069128; ENSRNOG00000011142.
GeneIDi80773.
KEGGirno:80773.
UCSCiRGD:621551. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12517 mRNA. Translation: CAA73117.1.
BC072535 mRNA. Translation: AAH72535.1.
RefSeqiNP_085075.1. NM_030586.2.
UniGeneiRn.10249.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AWPX-ray2.00A/B13-103[»]
1B5MX-ray2.70A19-102[»]
1EUEX-ray1.80A/B17-102[»]
1ICCX-ray2.00A/B/C/D17-103[»]
1LJ0X-ray2.00A/B/C/D12-103[»]
2I89X-ray2.10A/B/C/D12-103[»]
3MUSX-ray2.00A/B17-102[»]
4HILX-ray1.25A/B17-102[»]
ProteinModelPortaliP04166.
SMRiP04166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996332.
STRINGi10116.ENSRNOP00000015006.

PTM databases

iPTMnetiP04166.
PhosphoSitePlusiP04166.
SwissPalmiP04166.

Proteomic databases

PaxDbiP04166.
PRIDEiP04166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015006; ENSRNOP00000015006; ENSRNOG00000011142.
ENSRNOT00000086435; ENSRNOP00000069128; ENSRNOG00000011142.
GeneIDi80773.
KEGGirno:80773.
UCSCiRGD:621551. rat.

Organism-specific databases

CTDi80777.
RGDi621551. Cyb5b.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiP04166.
OMAiYYMAESK.
OrthoDBiEOG091G17G8.
PhylomeDBiP04166.
TreeFamiTF314537.

Miscellaneous databases

EvolutionaryTraceiP04166.
PROiP04166.

Gene expression databases

BgeeiENSRNOG00000011142.
GenevisibleiP04166. RN.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
3.10.120.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5B_RAT
AccessioniPrimary (citable) accession number: P04166
Secondary accession number(s): Q9QWG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.