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Protein

Cytochrome b5 type B

Gene

Cyb5b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Iron (heme axial ligand)1
Metal bindingi79Iron (heme axial ligand)1

GO - Molecular functioni

  • electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity Source: RGD
  • enzyme activator activity Source: RGD
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-211945 Phase I - Functionalization of compounds

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5 type B
Alternative name(s):
Cytochrome b5 outer mitochondrial membrane isoform
Gene namesi
Name:Cyb5b
Synonyms:Cyb5m, Omb5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi621551 Cyb5b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei119 – 136HelicalSequence analysisAdd BLAST18

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00000064772 – 111 Publication10
ChainiPRO_000000647812 – 146Cytochrome b5 type BAdd BLAST135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei30N6-acetyllysineBy similarity1
Modified residuei80PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04166
PRIDEiP04166

PTM databases

iPTMnetiP04166
PhosphoSitePlusiP04166
SwissPalmiP04166

Expressioni

Gene expression databases

BgeeiENSRNOG00000011142
GenevisibleiP04166 RN

Interactioni

Subunit structurei

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015006

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 28Combined sources4
Beta strandi36 – 41Combined sources6
Beta strandi44 – 47Combined sources4
Helixi49 – 51Combined sources3
Turni52 – 54Combined sources3
Helixi60 – 63Combined sources4
Turni64 – 67Combined sources4
Helixi71 – 77Combined sources7
Helixi81 – 85Combined sources5
Helixi86 – 89Combined sources4
Beta strandi91 – 95Combined sources5
Helixi97 – 99Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AWPX-ray2.00A/B13-103[»]
1B5MX-ray2.70A19-102[»]
1EUEX-ray1.80A/B17-102[»]
1ICCX-ray2.00A/B/C/D17-103[»]
1LJ0X-ray2.00A/B/C/D12-103[»]
2I89X-ray2.10A/B/C/D12-103[»]
3MUSX-ray2.00A/B17-102[»]
4HILX-ray1.25A/B17-102[»]
ProteinModelPortaliP04166
SMRiP04166
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04166

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 96Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537 Eukaryota
COG5274 LUCA
GeneTreeiENSGT00510000046507
HOGENOMiHOG000039853
HOVERGENiHBG002653
InParanoidiP04166
OMAiYYMAESK
OrthoDBiEOG091G17G8
PhylomeDBiP04166
TreeFamiTF314537

Family and domain databases

Gene3Di3.10.120.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PRINTSiPR00363 CYTOCHROMEB5
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPEASGSG RNGQGSDPAV TYYRLEEVAK RNTAEETWMV IHGRVYDITR
60 70 80 90 100
FLSEHPGGEE VLLEQAGADA TESFEDVGHS PDAREMLKQY YIGDVHPNDL
110 120 130 140
KPKDGDKDPS KNNSCQSSWA YWIVPIVGAI LIGFLYRHFW ADSKSS
Length:146
Mass (Da):16,265
Last modified:January 11, 2001 - v2
Checksum:i1CA90DD3C81C412E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12N → D AA sequence (PubMed:6840088).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12517 mRNA Translation: CAA73117.1
BC072535 mRNA Translation: AAH72535.1
RefSeqiNP_085075.1, NM_030586.2
UniGeneiRn.10249

Genome annotation databases

EnsembliENSRNOT00000015006; ENSRNOP00000015006; ENSRNOG00000011142
ENSRNOT00000086435; ENSRNOP00000069128; ENSRNOG00000011142
GeneIDi80773
KEGGirno:80773
UCSCiRGD:621551 rat

Similar proteinsi

Entry informationi

Entry nameiCYB5B_RAT
AccessioniPrimary (citable) accession number: P04166
Secondary accession number(s): Q9QWG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: January 11, 2001
Last modified: May 23, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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