P04161 (PUR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART

Gene names

Name:	ADE8
Ordered Locus Names:	YDR408C
ORF Names:	D9509.26

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Miscellaneous	Present with 3910 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro adenine biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylglycinamide formyltransferase activity Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Phosphoribosylglycinamide formyltransferase		PRO_0000074954
Regions
	Region	12 – 14	3	5'-phosphoribosylglycinamide binding By similarity
	Region	105 – 108	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	125	1	Proton donor By similarity
	Binding site	123	1	10-formyltetrahydrofolate By similarity
	Binding site	167	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	197	1	5'-phosphoribosylglycinamide By similarity
	Site	167	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04161 [UniParc]. Last modified November 1, 1986. Version 1. Checksum: 4FC8105E35A0A2A6	FASTA	214	23,540
	10 20 30 40 50 60 MARIVVLISG SGSNLQALID AQKQGQLGED AHIVSVISSS KKAYGLTRAA DNNIPTKVCS 70 80 90 100 110 120 LYPYTKGIAK EDKAARAKAR SQFENDLAKL VLEEKPDVII CAGWLLILGS TFLSQLQSVP 130 140 150 160 170 180 ILNLHPALPG CFDGTTHAIE MAWRKCQDEN KPLTAGCMVH YVIEEVDKGE PLVVKKLEII 190 200 210 PGEETLEQYE QRVHDAEHIA IVEATYKVLQ QLHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate." White J.H., Lusnak K., Fogel S. Nature 315:350-352(1985) [PubMed: 3889658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36585 Genomic DNA. Translation: AAA34406.1. U32274 Genomic DNA. Translation: AAB64848.1. BK006938 Genomic DNA. Translation: DAA12250.1.
PIR	A8BYD. A22316.
RefSeq	NP_010696.1. NM_001180716.1.
3D structure databases
ProteinModelPortal	P04161.
SMR	P04161. Positions 2-212.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1177N.
IntAct	P04161. 4 interactions.
MINT	MINT-390623.
STRING	P04161.
Proteomic databases
PeptideAtlas	P04161.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR408C; YDR408C; YDR408C.
GeneID	852017.
KEGG	sce:YDR408C.
NMPDR	fig|4932.3.peg.1469.
Organism-specific databases
CYGD	YDR408c.
SGD	S000002816. ADE8.
Phylogenomic databases
eggNOG	fuNOG10564.
GeneTree	EFGT00050000003453.
HOGENOM	HBG649382.
OMA	CAGWMLI.
OrthoDB	EOG46DQC3.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-485.
Gene expression databases
ArrayExpress	P04161.
Genevestigator	P04161.
GermOnline	YDR408C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00601.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970228.

Entry information

Entry name

PUR3_YEAST

Accession

Primary (citable) accession number: P04161
Secondary accession number(s): D6VT40

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	November 1, 1986
Last modified:	December 14, 2011
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program