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Protein

Phosphoribosylglycinamide formyltransferase

Gene

ADE8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoribosylglycinamide formyltransferase (ADE8)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 123110-formyltetrahydrofolateBy similarity
Active sitei125 – 1251Proton donorBy similarity
Binding sitei167 – 167110-formyltetrahydrofolate; via amide nitrogenBy similarity
Sitei167 – 1671Raises pKa of active site HisBy similarity
Binding sitei197 – 19715'-phosphoribosylglycinamideBy similarity

GO - Molecular functioni

  • phosphoribosylglycinamide formyltransferase activity Source: UniProtKB

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: SGD
  • adenine biosynthetic process Source: SGD
  • purine nucleotide biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-485.
YEAST:YDR408C-MONOMER.
UniPathwayiUPA00074; UER00126.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:ADE8
Ordered Locus Names:YDR408C
ORF Names:D9509.26
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR408C.
SGDiS000002816. ADE8.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 214214Phosphoribosylglycinamide formyltransferasePRO_0000074954Add
BLAST

Proteomic databases

MaxQBiP04161.
PeptideAtlasiP04161.

Interactioni

Protein-protein interaction databases

BioGridi32468. 19 interactions.
DIPiDIP-1177N.
IntActiP04161. 5 interactions.
MINTiMINT-390623.

Structurei

3D structure databases

ProteinModelPortaliP04161.
SMRiP04161. Positions 4-199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 1435'-phosphoribosylglycinamide bindingBy similarity
Regioni105 – 108410-formyltetrahydrofolate bindingBy similarity

Sequence similaritiesi

Belongs to the GART family.Curated

Phylogenomic databases

HOGENOMiHOG000033575.
InParanoidiP04161.
KOiK00601.
OMAiLAGWMHV.
OrthoDBiEOG7ZPNX0.

Family and domain databases

Gene3Di3.40.50.170. 1 hit.
HAMAPiMF_01930. PurN.
InterProiIPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR004607. PurN_trans.
[Graphical view]
PfamiPF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00639. PurN. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARIVVLISG SGSNLQALID AQKQGQLGED AHIVSVISSS KKAYGLTRAA
60 70 80 90 100
DNNIPTKVCS LYPYTKGIAK EDKAARAKAR SQFENDLAKL VLEEKPDVII
110 120 130 140 150
CAGWLLILGS TFLSQLQSVP ILNLHPALPG CFDGTTHAIE MAWRKCQDEN
160 170 180 190 200
KPLTAGCMVH YVIEEVDKGE PLVVKKLEII PGEETLEQYE QRVHDAEHIA
210
IVEATYKVLQ QLHK
Length:214
Mass (Da):23,540
Last modified:November 1, 1986 - v1
Checksum:i4FC8105E35A0A2A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36585 Genomic DNA. Translation: AAA34406.1.
U32274 Genomic DNA. Translation: AAB64848.1.
BK006938 Genomic DNA. Translation: DAA12250.1.
PIRiA22316. A8BYD.
RefSeqiNP_010696.3. NM_001180716.3.

Genome annotation databases

EnsemblFungiiYDR408C; YDR408C; YDR408C.
GeneIDi852017.
KEGGisce:YDR408C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36585 Genomic DNA. Translation: AAA34406.1.
U32274 Genomic DNA. Translation: AAB64848.1.
BK006938 Genomic DNA. Translation: DAA12250.1.
PIRiA22316. A8BYD.
RefSeqiNP_010696.3. NM_001180716.3.

3D structure databases

ProteinModelPortaliP04161.
SMRiP04161. Positions 4-199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32468. 19 interactions.
DIPiDIP-1177N.
IntActiP04161. 5 interactions.
MINTiMINT-390623.

Proteomic databases

MaxQBiP04161.
PeptideAtlasiP04161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR408C; YDR408C; YDR408C.
GeneIDi852017.
KEGGisce:YDR408C.

Organism-specific databases

EuPathDBiFungiDB:YDR408C.
SGDiS000002816. ADE8.

Phylogenomic databases

HOGENOMiHOG000033575.
InParanoidiP04161.
KOiK00601.
OMAiLAGWMHV.
OrthoDBiEOG7ZPNX0.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00126.
BioCyciMetaCyc:MONOMER-485.
YEAST:YDR408C-MONOMER.

Miscellaneous databases

PROiP04161.

Family and domain databases

Gene3Di3.40.50.170. 1 hit.
HAMAPiMF_01930. PurN.
InterProiIPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR004607. PurN_trans.
[Graphical view]
PfamiPF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00639. PurN. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate."
    White J.H., Lusnak K., Fogel S.
    Nature 315:350-352(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUR3_YEAST
AccessioniPrimary (citable) accession number: P04161
Secondary accession number(s): D6VT40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.