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Protein

Receptor-type tyrosine-protein phosphatase C

Gene

Ptprc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei790SubstrateBy similarity1
Active sitei822Phosphocysteine intermediateBy similarity1
Binding sitei866SubstrateBy similarity1
Active sitei1137Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB
  • protein kinase regulator activity Source: RGD
  • protein tyrosine phosphatase activity Source: UniProtKB
  • transmembrane receptor protein tyrosine phosphatase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase C (EC:3.1.3.48)
Alternative name(s):
Leukocyte common antigen
Short name:
L-CA
T200
CD_antigen: CD45
Gene namesi
Name:Ptprc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi3451. Ptprc.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 546ExtracellularSequence analysisAdd BLAST523
Transmembranei547 – 567HelicalSequence analysisAdd BLAST21
Topological domaini568 – 1273CytoplasmicSequence analysisAdd BLAST706

GO - Cellular componenti

  • cell surface Source: RGD
  • cytoplasmic side of plasma membrane Source: RGD
  • focal adhesion Source: UniProtKB
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • membrane raft Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000002547224 – 1273Receptor-type tyrosine-protein phosphatase CAdd BLAST1250

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62N-linked (GlcNAc...)Sequence analysis1
Glycosylationi142N-linked (GlcNAc...)Sequence analysis1
Glycosylationi153N-linked (GlcNAc...)Sequence analysis1
Glycosylationi164N-linked (GlcNAc...)Sequence analysis1
Glycosylationi178N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi245N-linked (GlcNAc...)Sequence analysis1
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi282N-linked (GlcNAc...)Sequence analysis1
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi333N-linked (GlcNAc...)Sequence analysis1
Glycosylationi371N-linked (GlcNAc...)Sequence analysis1
Glycosylationi374N-linked (GlcNAc...)Sequence analysis1
Glycosylationi471N-linked (GlcNAc...)Sequence analysis1
Glycosylationi502N-linked (GlcNAc...)Sequence analysis1
Modified residuei652PhosphotyrosineCombined sources1
Modified residuei944PhosphoserineBy similarity1
Modified residuei963PhosphoserineCombined sources1
Modified residuei966PhosphoserineCombined sources1
Modified residuei970PhosphoserineCombined sources1
Modified residuei973PhosphoserineCombined sources1
Modified residuei974PhosphoserineCombined sources1
Modified residuei978PhosphoserineCombined sources1
Modified residuei1209PhosphoserineCombined sources1
Modified residuei1266PhosphoserineBy similarity1

Post-translational modificationi

Heavily N- and O-glycosylated.
The cytoplasmic domain contains potential phosphorylation sites.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP04157.
PRIDEiP04157.

PTM databases

iPTMnetiP04157.
PhosphoSitePlusiP04157.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are found in thymocyte and lymph node. Isoform 4 and isoform 3 are found in the lymph nod.

Gene expression databases

BgeeiENSRNOG00000000655.
GenevisibleiP04157. RN.

Interactioni

Subunit structurei

Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Binds GANAB and PRKCSH (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246829. 1 interactor.
IntActiP04157. 2 interactors.
STRINGi10116.ENSRNOP00000063859.

Structurei

Secondary structure

11273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi385 – 387Combined sources3
Beta strandi423 – 426Combined sources4
Beta strandi445 – 448Combined sources4
Beta strandi458 – 464Combined sources7
Beta strandi466 – 468Combined sources3
Beta strandi470 – 475Combined sources6
Beta strandi486 – 493Combined sources8
Beta strandi496 – 510Combined sources15
Beta strandi518 – 526Combined sources9
Beta strandi535 – 540Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FN8X-ray2.45A/B357-546[»]
ProteinModelPortaliP04157.
SMRiP04157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini361 – 452Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST92
Domaini453 – 545Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST93
Domaini622 – 881Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST260
Domaini913 – 1196Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST284

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni822 – 828Substrate bindingBy similarity7

Domaini

The first PTPase domain interacts with SKAP1.By similarity

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000049064.
HOVERGENiHBG000066.
InParanoidiP04157.
KOiK06478.
OMAiKHELEMS.
OrthoDBiEOG091G015A.
PhylomeDBiP04157.
TreeFamiTF351829.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 2 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR024739. PTP_recept_N.
IPR000242. PTPase_domain.
IPR016335. Ptprc.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF12567. CD45. 1 hit.
PF00041. fn3. 1 hit.
PF12453. PTP_N. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF002004. Leukocyte_common_antigen. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 2 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P04157-1) [UniParc]FASTAAdd to basket
Also known as: L-CA variant 4

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYLWLKLLAF SLALLGPEVF VTGQGTTDDG LDTTEIVLLP QTDPLPARTT
60 70 80 90 100
EFTPPSISER GNGSSETTYL PGFSSTLMPH LTPQPDSQTP SARGADTQTL
110 120 130 140 150
SSQADLTTLT AAPSGETDPP GVPEESTVPE TFPGGTPILA RNSTAPSPTH
160 170 180 190 200
TSNVSTTDIS SGANLTTPAP STLGFASNTT TSTEIATPQT KPSCDEKFGN
210 220 230 240 250
VTVRYIYDDS SKNFNANLEG DKKPKCEYTD CEKELKNLPE CSQKNVTLSN
260 270 280 290 300
GSCTPDKIIN LDVPPGTHNF NLTNCTPDIE ANTSICLEWK IKNKFTCDIQ
310 320 330 340 350
KISYNFRCTP EMKTFALDKH GTLWLHNLTV RTNYTCAAEV LYNNVILLKQ
360 370 380 390 400
DRRVQTDFGT PEMLPHVQCK NSTNSTTLVS WAEPASKHHG YILCYKKTPS
410 420 430 440 450
EKCENLANDV NSFEVKNLRP YTEYTVSLFA YVIGRVQRNG PAKDCNFRTK
460 470 480 490 500
AARPGKVNGM KTSRASDNSI NVTCNSPYEI NGPEARYILE VKSGGSLVKT
510 520 530 540 550
FNQSTCKFVV DNLYYSTDYE FLVYFYNGEY LGDPEIKPQS TSYNSKALII
560 570 580 590 600
FLVFLIIVTS IALLVVLYKI YDLRKKRSSN LDEQQELVER DEEKQLINVD
610 620 630 640 650
PIHSDLLLET YKRKIADEGR LFLAEFQSIP RVFSKFPIKD ARKSQNQNKN
660 670 680 690 700
RYVDILPYDY NRVELSEING DAGSTYINAS YIDGFKEPRK YIAAQGPRDE
710 720 730 740 750
TVDDFWKMIW EQKATVIVMV TRCEEGNRNK CAEYWPCMEE GTRTFRDVVV
760 770 780 790 800
TINDHKRCPD YIIQKLSIAH KKEKATGREV THIQFTSWPD HGVPEDPHLL
810 820 830 840 850
LKLRRRVNAF SNFFSGPIVV HCSAGVGRTG TYIGIDAMLE SLEAEGKVDV
860 870 880 890 900
YGYVVNLRRQ RCLMVQVEAQ YILIHQALVE YNQFGETEVN LSELHSCLQN
910 920 930 940 950
LKKRDPPSDP SPLEAEYQRL PSYRSWRTQH IGNQEENKKK NRSSNVVPYD
960 970 980 990 1000
FNRVPLKHEL EMSKESEAES DESSDEDSDS EETSKYINAS FVMSYWKPEM
1010 1020 1030 1040 1050
MIAAQGPLKE TIGDFWQMIF QRKVKVIVML TELMSGDQEV CAQYWGEGKQ
1060 1070 1080 1090 1100
TYGDMEVMLK DTNKSSAYIL RAFELRHSKR KEPRTVYQYQ CTTWKGEELP
1110 1120 1130 1140 1150
AEPKDLVTLI QNIKQKLPKS GSEGMKYHKH ASILVHCRDG SQQTGLFCAL
1160 1170 1180 1190 1200
FNLLESAETE DVVDVFQVVK SLRKARPGMV GSFEQYQFLY DIMASIYPTQ
1210 1220 1230 1240 1250
NGQVKKANSQ DKIEFHNEVD GAKQDANCVQ PADPLNKAQE DSKEVGASEP
1260 1270
ASGSEEPEHS ANGPMSPALT PSS
Length:1,273
Mass (Da):143,269
Last modified:June 12, 2007 - v2
Checksum:iEDFA48100ACF2CB2
GO
Isoform 2 (identifier: P04157-2) [UniParc]FASTAAdd to basket
Also known as: L-CA variant 1

The sequence of this isoform differs from the canonical sequence as follows:
     30-161: Missing.

Show »
Length:1,141
Mass (Da):129,697
Checksum:iD8CD289BA1873388
GO
Isoform 3 (identifier: P04157-3) [UniParc]FASTAAdd to basket
Also known as: L-CA variant 2

The sequence of this isoform differs from the canonical sequence as follows:
     30-120: Missing.

Show »
Length:1,182
Mass (Da):133,819
Checksum:iEDF2153D0EBBF86E
GO
Isoform 4 (identifier: P04157-4) [UniParc]FASTAAdd to basket
Also known as: L-CA variant 3

The sequence of this isoform differs from the canonical sequence as follows:
     30-71: Missing.
     121-161: Missing.

Show »
Length:1,190
Mass (Da):134,662
Checksum:i61CD4F787C7E695F
GO
Isoform 5 (identifier: P04157-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-161: Missing.

Show »
Length:1,183
Mass (Da):134,182
Checksum:i1076BD878F13B5CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56S → R in AAA41518 (Ref. 3) Curated1
Sequence conflicti56S → R in AAA41521 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00516730 – 161Missing in isoform 2. 1 PublicationAdd BLAST132
Alternative sequenceiVSP_00516630 – 120Missing in isoform 3. 2 PublicationsAdd BLAST91
Alternative sequenceiVSP_00516530 – 71Missing in isoform 4. 3 PublicationsAdd BLAST42
Alternative sequenceiVSP_02616372 – 161Missing in isoform 5. 1 PublicationAdd BLAST90
Alternative sequenceiVSP_005168121 – 161Missing in isoform 4. 3 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CB793707 mRNA. No translation available.
Y00065 mRNA. Translation: CAA68272.1.
Y00065 mRNA. Translation: CAA68273.1.
Y00065 mRNA. Translation: CAA68274.1.
Y00065 mRNA. Translation: CAA68275.1.
M25820 mRNA. Translation: AAA41518.1.
M25821 mRNA. Translation: AAA41519.1.
M25822 mRNA. Translation: AAA41520.1.
M25823 mRNA. Translation: AAA41521.1.
PIRiA45854.
RefSeqiNP_001103357.1. NM_001109887.2. [P04157-4]
NP_001103358.1. NM_001109888.2. [P04157-5]
NP_001103359.1. NM_001109889.2. [P04157-3]
NP_001103360.1. NM_001109890.2. [P04157-2]
NP_612516.2. NM_138507.3. [P04157-1]
XP_006249972.1. XM_006249910.2. [P04157-1]
UniGeneiRn.90166.

Genome annotation databases

EnsembliENSRNOT00000000814; ENSRNOP00000000814; ENSRNOG00000000655. [P04157-2]
ENSRNOT00000029865; ENSRNOP00000029889; ENSRNOG00000000655. [P04157-5]
ENSRNOT00000029878; ENSRNOP00000030503; ENSRNOG00000000655. [P04157-4]
ENSRNOT00000060292; ENSRNOP00000057042; ENSRNOG00000000655. [P04157-3]
ENSRNOT00000064785; ENSRNOP00000063859; ENSRNOG00000000655. [P04157-1]
GeneIDi24699.
KEGGirno:24699.
UCSCiRGD:3451. rat. [P04157-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CB793707 mRNA. No translation available.
Y00065 mRNA. Translation: CAA68272.1.
Y00065 mRNA. Translation: CAA68273.1.
Y00065 mRNA. Translation: CAA68274.1.
Y00065 mRNA. Translation: CAA68275.1.
M25820 mRNA. Translation: AAA41518.1.
M25821 mRNA. Translation: AAA41519.1.
M25822 mRNA. Translation: AAA41520.1.
M25823 mRNA. Translation: AAA41521.1.
PIRiA45854.
RefSeqiNP_001103357.1. NM_001109887.2. [P04157-4]
NP_001103358.1. NM_001109888.2. [P04157-5]
NP_001103359.1. NM_001109889.2. [P04157-3]
NP_001103360.1. NM_001109890.2. [P04157-2]
NP_612516.2. NM_138507.3. [P04157-1]
XP_006249972.1. XM_006249910.2. [P04157-1]
UniGeneiRn.90166.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FN8X-ray2.45A/B357-546[»]
ProteinModelPortaliP04157.
SMRiP04157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246829. 1 interactor.
IntActiP04157. 2 interactors.
STRINGi10116.ENSRNOP00000063859.

PTM databases

iPTMnetiP04157.
PhosphoSitePlusiP04157.

Proteomic databases

PaxDbiP04157.
PRIDEiP04157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000814; ENSRNOP00000000814; ENSRNOG00000000655. [P04157-2]
ENSRNOT00000029865; ENSRNOP00000029889; ENSRNOG00000000655. [P04157-5]
ENSRNOT00000029878; ENSRNOP00000030503; ENSRNOG00000000655. [P04157-4]
ENSRNOT00000060292; ENSRNOP00000057042; ENSRNOG00000000655. [P04157-3]
ENSRNOT00000064785; ENSRNOP00000063859; ENSRNOG00000000655. [P04157-1]
GeneIDi24699.
KEGGirno:24699.
UCSCiRGD:3451. rat. [P04157-1]

Organism-specific databases

CTDi5788.
RGDi3451. Ptprc.

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000049064.
HOVERGENiHBG000066.
InParanoidiP04157.
KOiK06478.
OMAiKHELEMS.
OrthoDBiEOG091G015A.
PhylomeDBiP04157.
TreeFamiTF351829.

Miscellaneous databases

PROiP04157.

Gene expression databases

BgeeiENSRNOG00000000655.
GenevisibleiP04157. RN.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di2.60.40.10. 2 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR024739. PTP_recept_N.
IPR000242. PTPase_domain.
IPR016335. Ptprc.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF12567. CD45. 1 hit.
PF00041. fn3. 1 hit.
PF12453. PTP_N. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF002004. Leukocyte_common_antigen. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 2 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRC_RAT
AccessioniPrimary (citable) accession number: P04157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: June 12, 2007
Last modified: November 30, 2016
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.