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Protein

Receptor-type tyrosine-protein phosphatase C

Gene

Ptprc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei790 – 7901SubstrateBy similarity
Active sitei822 – 8221Phosphocysteine intermediateBy similarity
Binding sitei866 – 8661SubstrateBy similarity
Active sitei1137 – 11371Phosphocysteine intermediateBy similarity

GO - Molecular functioni

  • heparin binding Source: Ensembl
  • protein kinase binding Source: UniProtKB
  • protein kinase regulator activity Source: RGD
  • protein tyrosine phosphatase activity Source: UniProtKB
  • transmembrane receptor protein tyrosine phosphatase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase C (EC:3.1.3.48)
Alternative name(s):
Leukocyte common antigen
Short name:
L-CA
T200
CD_antigen: CD45
Gene namesi
Name:Ptprc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi3451. Ptprc.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 546523ExtracellularSequence AnalysisAdd
BLAST
Transmembranei547 – 56721HelicalSequence AnalysisAdd
BLAST
Topological domaini568 – 1273706CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: RGD
  • cytoplasmic side of plasma membrane Source: RGD
  • external side of plasma membrane Source: Ensembl
  • focal adhesion Source: UniProtKB
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: UniProtKB
  • intracellular Source: GOC
  • membrane raft Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 12731250Receptor-type tyrosine-protein phosphatase CPRO_0000025472Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi471 – 4711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
Modified residuei944 – 9441PhosphoserineBy similarity
Modified residuei1266 – 12661PhosphoserineBy similarity

Post-translational modificationi

Heavily N- and O-glycosylated.
The cytoplasmic domain contains potential phosphorylation sites.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP04157.
PRIDEiP04157.

PTM databases

PhosphoSiteiP04157.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are found in thymocyte and lymph node. Isoform 4 and isoform 3 are found in the lymph nod.

Gene expression databases

ExpressionAtlasiP04157. baseline and differential.
GenevisibleiP04157. RN.

Interactioni

Subunit structurei

Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Binds GANAB and PRKCSH (By similarity).By similarity

Protein-protein interaction databases

BioGridi246829. 1 interaction.
IntActiP04157. 2 interactions.
STRINGi10116.ENSRNOP00000063859.

Structurei

3D structure databases

ProteinModelPortaliP04157.
SMRiP04157. Positions 594-1198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini361 – 45292Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini453 – 54593Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini622 – 881260Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd
BLAST
Domaini913 – 1196284Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni822 – 8287Substrate bindingBy similarity

Domaini

The first PTPase domain interacts with SKAP1.By similarity

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000049064.
HOVERGENiHBG000066.
InParanoidiP04157.
KOiK06478.
OMAiKHELEMS.
OrthoDBiEOG7XSTCW.
PhylomeDBiP04157.
TreeFamiTF351829.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR024739. PTP_recept_N.
IPR016335. Ptprc.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PANTHERiPTHR19134:SF278. PTHR19134:SF278. 1 hit.
PfamiPF12567. CD45. 1 hit.
PF00041. fn3. 1 hit.
PF12453. PTP_N. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF002004. Leukocyte_common_antigen. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 2 hits.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P04157-1) [UniParc]FASTAAdd to basket

Also known as: L-CA variant 4

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYLWLKLLAF SLALLGPEVF VTGQGTTDDG LDTTEIVLLP QTDPLPARTT
60 70 80 90 100
EFTPPSISER GNGSSETTYL PGFSSTLMPH LTPQPDSQTP SARGADTQTL
110 120 130 140 150
SSQADLTTLT AAPSGETDPP GVPEESTVPE TFPGGTPILA RNSTAPSPTH
160 170 180 190 200
TSNVSTTDIS SGANLTTPAP STLGFASNTT TSTEIATPQT KPSCDEKFGN
210 220 230 240 250
VTVRYIYDDS SKNFNANLEG DKKPKCEYTD CEKELKNLPE CSQKNVTLSN
260 270 280 290 300
GSCTPDKIIN LDVPPGTHNF NLTNCTPDIE ANTSICLEWK IKNKFTCDIQ
310 320 330 340 350
KISYNFRCTP EMKTFALDKH GTLWLHNLTV RTNYTCAAEV LYNNVILLKQ
360 370 380 390 400
DRRVQTDFGT PEMLPHVQCK NSTNSTTLVS WAEPASKHHG YILCYKKTPS
410 420 430 440 450
EKCENLANDV NSFEVKNLRP YTEYTVSLFA YVIGRVQRNG PAKDCNFRTK
460 470 480 490 500
AARPGKVNGM KTSRASDNSI NVTCNSPYEI NGPEARYILE VKSGGSLVKT
510 520 530 540 550
FNQSTCKFVV DNLYYSTDYE FLVYFYNGEY LGDPEIKPQS TSYNSKALII
560 570 580 590 600
FLVFLIIVTS IALLVVLYKI YDLRKKRSSN LDEQQELVER DEEKQLINVD
610 620 630 640 650
PIHSDLLLET YKRKIADEGR LFLAEFQSIP RVFSKFPIKD ARKSQNQNKN
660 670 680 690 700
RYVDILPYDY NRVELSEING DAGSTYINAS YIDGFKEPRK YIAAQGPRDE
710 720 730 740 750
TVDDFWKMIW EQKATVIVMV TRCEEGNRNK CAEYWPCMEE GTRTFRDVVV
760 770 780 790 800
TINDHKRCPD YIIQKLSIAH KKEKATGREV THIQFTSWPD HGVPEDPHLL
810 820 830 840 850
LKLRRRVNAF SNFFSGPIVV HCSAGVGRTG TYIGIDAMLE SLEAEGKVDV
860 870 880 890 900
YGYVVNLRRQ RCLMVQVEAQ YILIHQALVE YNQFGETEVN LSELHSCLQN
910 920 930 940 950
LKKRDPPSDP SPLEAEYQRL PSYRSWRTQH IGNQEENKKK NRSSNVVPYD
960 970 980 990 1000
FNRVPLKHEL EMSKESEAES DESSDEDSDS EETSKYINAS FVMSYWKPEM
1010 1020 1030 1040 1050
MIAAQGPLKE TIGDFWQMIF QRKVKVIVML TELMSGDQEV CAQYWGEGKQ
1060 1070 1080 1090 1100
TYGDMEVMLK DTNKSSAYIL RAFELRHSKR KEPRTVYQYQ CTTWKGEELP
1110 1120 1130 1140 1150
AEPKDLVTLI QNIKQKLPKS GSEGMKYHKH ASILVHCRDG SQQTGLFCAL
1160 1170 1180 1190 1200
FNLLESAETE DVVDVFQVVK SLRKARPGMV GSFEQYQFLY DIMASIYPTQ
1210 1220 1230 1240 1250
NGQVKKANSQ DKIEFHNEVD GAKQDANCVQ PADPLNKAQE DSKEVGASEP
1260 1270
ASGSEEPEHS ANGPMSPALT PSS
Length:1,273
Mass (Da):143,269
Last modified:June 12, 2007 - v2
Checksum:iEDFA48100ACF2CB2
GO
Isoform 2 (identifier: P04157-2) [UniParc]FASTAAdd to basket

Also known as: L-CA variant 1

The sequence of this isoform differs from the canonical sequence as follows:
     30-161: Missing.

Show »
Length:1,141
Mass (Da):129,697
Checksum:iD8CD289BA1873388
GO
Isoform 3 (identifier: P04157-3) [UniParc]FASTAAdd to basket

Also known as: L-CA variant 2

The sequence of this isoform differs from the canonical sequence as follows:
     30-120: Missing.

Show »
Length:1,182
Mass (Da):133,819
Checksum:iEDF2153D0EBBF86E
GO
Isoform 4 (identifier: P04157-4) [UniParc]FASTAAdd to basket

Also known as: L-CA variant 3

The sequence of this isoform differs from the canonical sequence as follows:
     30-71: Missing.
     121-161: Missing.

Show »
Length:1,190
Mass (Da):134,662
Checksum:i61CD4F787C7E695F
GO
Isoform 5 (identifier: P04157-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     72-161: Missing.

Show »
Length:1,183
Mass (Da):134,182
Checksum:i1076BD878F13B5CA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561S → R in AAA41518 (Ref. 3) Curated
Sequence conflicti56 – 561S → R in AAA41521 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei30 – 161132Missing in isoform 2. 1 PublicationVSP_005167Add
BLAST
Alternative sequencei30 – 12091Missing in isoform 3. 2 PublicationsVSP_005166Add
BLAST
Alternative sequencei30 – 7142Missing in isoform 4. 3 PublicationsVSP_005165Add
BLAST
Alternative sequencei72 – 16190Missing in isoform 5. 1 PublicationVSP_026163Add
BLAST
Alternative sequencei121 – 16141Missing in isoform 4. 3 PublicationsVSP_005168Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CB793707 mRNA. No translation available.
Y00065 mRNA. Translation: CAA68272.1.
Y00065 mRNA. Translation: CAA68273.1.
Y00065 mRNA. Translation: CAA68274.1.
Y00065 mRNA. Translation: CAA68275.1.
M25820 mRNA. Translation: AAA41518.1.
M25821 mRNA. Translation: AAA41519.1.
M25822 mRNA. Translation: AAA41520.1.
M25823 mRNA. Translation: AAA41521.1.
PIRiA45854.
RefSeqiNP_001103357.1. NM_001109887.1. [P04157-4]
NP_001103358.1. NM_001109888.1. [P04157-5]
NP_001103359.1. NM_001109889.1. [P04157-3]
NP_001103360.1. NM_001109890.1. [P04157-2]
NP_612516.2. NM_138507.2. [P04157-1]
XP_006249972.1. XM_006249910.2. [P04157-1]
UniGeneiRn.90166.

Genome annotation databases

EnsembliENSRNOT00000000814; ENSRNOP00000000814; ENSRNOG00000000655. [P04157-2]
ENSRNOT00000029865; ENSRNOP00000029889; ENSRNOG00000000655. [P04157-5]
ENSRNOT00000029878; ENSRNOP00000030503; ENSRNOG00000000655. [P04157-4]
ENSRNOT00000060292; ENSRNOP00000057042; ENSRNOG00000000655. [P04157-3]
ENSRNOT00000064785; ENSRNOP00000063859; ENSRNOG00000000655. [P04157-1]
GeneIDi24699.
KEGGirno:24699.
UCSCiRGD:3451. rat. [P04157-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CB793707 mRNA. No translation available.
Y00065 mRNA. Translation: CAA68272.1.
Y00065 mRNA. Translation: CAA68273.1.
Y00065 mRNA. Translation: CAA68274.1.
Y00065 mRNA. Translation: CAA68275.1.
M25820 mRNA. Translation: AAA41518.1.
M25821 mRNA. Translation: AAA41519.1.
M25822 mRNA. Translation: AAA41520.1.
M25823 mRNA. Translation: AAA41521.1.
PIRiA45854.
RefSeqiNP_001103357.1. NM_001109887.1. [P04157-4]
NP_001103358.1. NM_001109888.1. [P04157-5]
NP_001103359.1. NM_001109889.1. [P04157-3]
NP_001103360.1. NM_001109890.1. [P04157-2]
NP_612516.2. NM_138507.2. [P04157-1]
XP_006249972.1. XM_006249910.2. [P04157-1]
UniGeneiRn.90166.

3D structure databases

ProteinModelPortaliP04157.
SMRiP04157. Positions 594-1198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246829. 1 interaction.
IntActiP04157. 2 interactions.
STRINGi10116.ENSRNOP00000063859.

Chemistry

GuidetoPHARMACOLOGYi1852.

PTM databases

PhosphoSiteiP04157.

Proteomic databases

PaxDbiP04157.
PRIDEiP04157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000814; ENSRNOP00000000814; ENSRNOG00000000655. [P04157-2]
ENSRNOT00000029865; ENSRNOP00000029889; ENSRNOG00000000655. [P04157-5]
ENSRNOT00000029878; ENSRNOP00000030503; ENSRNOG00000000655. [P04157-4]
ENSRNOT00000060292; ENSRNOP00000057042; ENSRNOG00000000655. [P04157-3]
ENSRNOT00000064785; ENSRNOP00000063859; ENSRNOG00000000655. [P04157-1]
GeneIDi24699.
KEGGirno:24699.
UCSCiRGD:3451. rat. [P04157-1]

Organism-specific databases

CTDi5788.
RGDi3451. Ptprc.

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000049064.
HOVERGENiHBG000066.
InParanoidiP04157.
KOiK06478.
OMAiKHELEMS.
OrthoDBiEOG7XSTCW.
PhylomeDBiP04157.
TreeFamiTF351829.

Miscellaneous databases

NextBioi604155.
PROiP04157.

Gene expression databases

ExpressionAtlasiP04157. baseline and differential.
GenevisibleiP04157. RN.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR024739. PTP_recept_N.
IPR016335. Ptprc.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PANTHERiPTHR19134:SF278. PTHR19134:SF278. 1 hit.
PfamiPF12567. CD45. 1 hit.
PF00041. fn3. 1 hit.
PF12453. PTP_N. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF002004. Leukocyte_common_antigen. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 2 hits.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Amgen rat EST program."
    Amgen EST program
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-147 (ISOFORM 4).
  2. "Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus."
    Barclay A.N., Jackson D.I., Willis A.C., Williams A.F.
    EMBO J. 6:1259-1264(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-218 (ISOFORMS 1; 2; 3 AND 4).
  3. Barclay A.N., Jackson D.I., Willis A.C., Williams A.F.
    Submitted (MAY-1987) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-1273 (ISOFORMS 1; 3; 4 AND 5).
  4. "Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 Mr."
    Thomas M.L., Barclay A.N., Gagnon J., Williams A.F.
    Cell 41:83-93(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 208-1255 (ISOFORM 1).

Entry informationi

Entry nameiPTPRC_RAT
AccessioniPrimary (citable) accession number: P04157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: June 12, 2007
Last modified: July 22, 2015
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.