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Protein

Protein UmuC

Gene

umuC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: InterPro

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: EcoliWiki
  2. single strand break repair Source: EcoCyc
  3. SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, SOS mutagenesis, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG11056-MONOMER.
ECOL316407:JW1173-MONOMER.
MetaCyc:EG11056-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UmuC
Gene namesi
Name:umuC
Ordered Locus Names:b1184, JW1173
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11056. umuC.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: EcoliWiki
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi75 – 751E → K in umuC36; non-mutable. 1 Publication
Mutagenesisi101 – 1011D → N in umuC104; non-mutable. 1 Publication
Mutagenesisi290 – 2901T → K in umuC25; non-mutable. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422Protein UmuCPRO_0000173982Add
BLAST

Proteomic databases

PRIDEiP04152.

Expressioni

Gene expression databases

GenevestigatoriP04152.

Interactioni

Protein-protein interaction databases

DIPiDIP-11090N.
IntActiP04152. 9 interactions.
MINTiMINT-1516814.
STRINGi511145.b1184.

Structurei

3D structure databases

ProteinModelPortaliP04152.
SMRiP04152. Positions 6-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 188187UmuCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 umuC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0389.
HOGENOMiHOG000252172.
InParanoidiP04152.
KOiK03502.
OMAiQETCVSR.
OrthoDBiEOG6CZQNH.
PhylomeDBiP04152.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR025188. DUF4113.
IPR024728. PolY_HhH_motif.
[Graphical view]
PfamiPF13438. DUF4113. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFALCDVNAF YASCETVFRP DLWGKPVVVL SNNDGCVIAR NAEAKALGVK
60 70 80 90 100
MGDPWFKQKD LFRRCGVVCF SSNYELYADM SNRVMSTLEE LSPRVEIYSI
110 120 130 140 150
DEAFCDLTGV RNCRDLTDFG REIRATVLQR THLTVGVGIA QTKTLAKLAN
160 170 180 190 200
HAAKKWQRQT GGVVDLSNLE RQRKLMSALP VDDVWGIGRR ISKKLDAMGI
210 220 230 240 250
KTVLDLADTD IRFIRKHFNV VLERTVRELR GEPCLQLEEF APTKQEIICS
260 270 280 290 300
RSFGERITDY PSMRQAICSY AARAAEKLRS EHQYCRFIST FIKTSPFALN
310 320 330 340 350
EPYYGNSASV KLLTPTQDSR DIINAATRSL DAIWQAGHRY QKAGVMLGDF
360 370 380 390 400
FSQGVAQLNL FDDNAPRPGS EQLMTVMDTL NAKEGRGTLY FAGQGIQQQW
410 420
QMKRAMLSPR YTTRSSDLLR VK
Length:422
Mass (Da):47,680
Last modified:November 1, 1997 - v2
Checksum:i0F1855D802A3F5F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521A → E in AAA24729 (PubMed:2989817).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA. Translation: AAA24729.1.
M13387 Genomic DNA. Translation: AAA98074.1.
U00096 Genomic DNA. Translation: AAC74268.1.
AP009048 Genomic DNA. Translation: BAA36031.1.
PIRiE64864. ZWECC.
RefSeqiNP_415702.1. NC_000913.3.
YP_489451.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74268; AAC74268; b1184.
BAA36031; BAA36031; BAA36031.
GeneIDi12932822.
946359.
KEGGiecj:Y75_p1156.
eco:b1184.
PATRICi32117614. VBIEscCol129921_1229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA. Translation: AAA24729.1.
M13387 Genomic DNA. Translation: AAA98074.1.
U00096 Genomic DNA. Translation: AAC74268.1.
AP009048 Genomic DNA. Translation: BAA36031.1.
PIRiE64864. ZWECC.
RefSeqiNP_415702.1. NC_000913.3.
YP_489451.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP04152.
SMRiP04152. Positions 6-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-11090N.
IntActiP04152. 9 interactions.
MINTiMINT-1516814.
STRINGi511145.b1184.

Proteomic databases

PRIDEiP04152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74268; AAC74268; b1184.
BAA36031; BAA36031; BAA36031.
GeneIDi12932822.
946359.
KEGGiecj:Y75_p1156.
eco:b1184.
PATRICi32117614. VBIEscCol129921_1229.

Organism-specific databases

EchoBASEiEB1049.
EcoGeneiEG11056. umuC.

Phylogenomic databases

eggNOGiCOG0389.
HOGENOMiHOG000252172.
InParanoidiP04152.
KOiK03502.
OMAiQETCVSR.
OrthoDBiEOG6CZQNH.
PhylomeDBiP04152.

Enzyme and pathway databases

BioCyciEcoCyc:EG11056-MONOMER.
ECOL316407:JW1173-MONOMER.
MetaCyc:EG11056-MONOMER.

Miscellaneous databases

PROiP04152.

Gene expression databases

GenevestigatoriP04152.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR001126. DNA_repair_prot_UmuC-like.
IPR017963. DNA_repair_prot_UmuC-like_N.
IPR025188. DUF4113.
IPR024728. PolY_HhH_motif.
[Graphical view]
PfamiPF13438. DUF4113. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli."
    Kitagawa Y., Akaboshi E., Shinagawa H., Horii T., Ogawa H., Kato T.
    Proc. Natl. Acad. Sci. U.S.A. 82:4336-4340(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology."
    Perry K.L., Elledge S.J., Mitchell B.B., Marsh L., Walker G.C.
    Proc. Natl. Acad. Sci. U.S.A. 82:4331-4335(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage."
    Koch W.H., Ennis D.G., Levine A.S., Woodgate R.
    Mol. Gen. Genet. 233:443-448(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiUMUC_ECOLI
AccessioniPrimary (citable) accession number: P04152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.