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Protein

Protein UmuC

Gene

umuC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in UV protection and mutation. Poorly processive, error-prone DNA polymerase involved in translesion repair (PubMed:10801133). Essential for induced (or SOS) mutagenesis. Able to replicate DNA across DNA lesions (thymine photodimers and abasic sites, translesion synthesis) in the presence of activated RecA; efficiency is maximal in the presence of the beta sliding-clamp and clamp-loading complex of DNA polymerase III plus single-stranded binding protein (SSB) (PubMed:10801133). RecA and to a lesser extent the beta clamp-complex may target Pol V to replication complexes stalled at DNA template lesions (PubMed:10801133).1 Publication

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • DNA-dependent ATPase activity Source: EcoCyc
  • DNA-directed DNA polymerase activity Source: EcoCyc
  • single-stranded DNA binding Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • SOS response Source: EcoCyc
  • translesion synthesis Source: EcoCyc

Keywordsi

Biological processDNA damage, DNA repair, SOS mutagenesis, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG11056-MONOMER.
MetaCyc:EG11056-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UmuC
Alternative name(s):
DNA polymerase V
Short name:
Pol V
Gene namesi
Name:umuC
Ordered Locus Names:b1184, JW1173
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11056. umuC.

Subcellular locationi

GO - Cellular componenti

  • DNA polymerase V complex Source: EcoCyc
  • integral component of membrane Source: EcoliWiki
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75E → K in umuC36; non-mutable. 1 Publication1
Mutagenesisi101D → N in umuC104; non-mutable. 1 Publication1
Mutagenesisi290T → K in umuC25; non-mutable. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739821 – 422Protein UmuCAdd BLAST422

Proteomic databases

PaxDbiP04152.
PRIDEiP04152.

Expressioni

Inductioni

Induced 1.5-fold by hydroxyurea.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261816. 142 interactors.
DIPiDIP-11090N.
IntActiP04152. 9 interactors.
MINTiMINT-1516814.
STRINGi316385.ECDH10B_1237.

Structurei

3D structure databases

ProteinModelPortaliP04152.
SMRiP04152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 188UmuCPROSITE-ProRule annotationAdd BLAST187

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated

Phylogenomic databases

eggNOGiENOG4105CQ3. Bacteria.
COG0389. LUCA.
HOGENOMiHOG000252172.
InParanoidiP04152.
KOiK03502.
PhylomeDBiP04152.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
InterProiView protein in InterPro
IPR017961. DNA_pol_Y-fam_little_finger.
IPR025188. DUF4113.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
PfamiView protein in Pfam
PF13438. DUF4113. 1 hit.
PF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiView protein in PROSITE
PS50173. UMUC. 1 hit.

Sequencei

Sequence statusi: Complete.

P04152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFALCDVNAF YASCETVFRP DLWGKPVVVL SNNDGCVIAR NAEAKALGVK
60 70 80 90 100
MGDPWFKQKD LFRRCGVVCF SSNYELYADM SNRVMSTLEE LSPRVEIYSI
110 120 130 140 150
DEAFCDLTGV RNCRDLTDFG REIRATVLQR THLTVGVGIA QTKTLAKLAN
160 170 180 190 200
HAAKKWQRQT GGVVDLSNLE RQRKLMSALP VDDVWGIGRR ISKKLDAMGI
210 220 230 240 250
KTVLDLADTD IRFIRKHFNV VLERTVRELR GEPCLQLEEF APTKQEIICS
260 270 280 290 300
RSFGERITDY PSMRQAICSY AARAAEKLRS EHQYCRFIST FIKTSPFALN
310 320 330 340 350
EPYYGNSASV KLLTPTQDSR DIINAATRSL DAIWQAGHRY QKAGVMLGDF
360 370 380 390 400
FSQGVAQLNL FDDNAPRPGS EQLMTVMDTL NAKEGRGTLY FAGQGIQQQW
410 420
QMKRAMLSPR YTTRSSDLLR VK
Length:422
Mass (Da):47,680
Last modified:November 1, 1997 - v2
Checksum:i0F1855D802A3F5F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152A → E in AAA24729 (PubMed:2989817).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA. Translation: AAA24729.1.
M13387 Genomic DNA. Translation: AAA98074.1.
U00096 Genomic DNA. Translation: AAC74268.1.
AP009048 Genomic DNA. Translation: BAA36031.1.
PIRiE64864. ZWECC.
RefSeqiNP_415702.1. NC_000913.3.
WP_000457616.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74268; AAC74268; b1184.
BAA36031; BAA36031; BAA36031.
GeneIDi946359.
KEGGiecj:JW1173.
eco:b1184.
PATRICifig|1411691.4.peg.1103.

Similar proteinsi

Entry informationi

Entry nameiUMUC_ECOLI
AccessioniPrimary (citable) accession number: P04152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families