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P04152

- UMUC_ECOLI

UniProt

P04152 - UMUC_ECOLI

Protein

Protein UmuC

Gene

umuC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. DNA-directed DNA polymerase activity Source: InterPro
    3. protein binding Source: EcoliWiki

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoliWiki
    2. single strand break repair Source: EcoCyc
    3. SOS response Source: UniProtKB-KW

    Keywords - Biological processi

    DNA damage, DNA repair, SOS mutagenesis, SOS response

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11056-MONOMER.
    ECOL316407:JW1173-MONOMER.
    MetaCyc:EG11056-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein UmuC
    Gene namesi
    Name:umuC
    Ordered Locus Names:b1184, JW1173
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11056. umuC.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: EcoliWiki

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi75 – 751E → K in umuC36; non-mutable. 1 Publication
    Mutagenesisi101 – 1011D → N in umuC104; non-mutable. 1 Publication
    Mutagenesisi290 – 2901T → K in umuC25; non-mutable. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 422422Protein UmuCPRO_0000173982Add
    BLAST

    Proteomic databases

    PRIDEiP04152.

    Expressioni

    Gene expression databases

    GenevestigatoriP04152.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-11090N.
    IntActiP04152. 9 interactions.
    MINTiMINT-1516814.
    STRINGi511145.b1184.

    Structurei

    3D structure databases

    ProteinModelPortaliP04152.
    SMRiP04152. Positions 1-351.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 188187UmuCPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-Y family.Curated
    Contains 1 umuC domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0389.
    HOGENOMiHOG000252172.
    KOiK03502.
    OMAiPMGAPYF.
    OrthoDBiEOG6CZQNH.
    PhylomeDBiP04152.

    Family and domain databases

    Gene3Di3.30.1490.100. 1 hit.
    InterProiIPR017961. DNA_pol_Y-fam_little_finger.
    IPR001126. DNA_repair_prot_UmuC-like.
    IPR017963. DNA_repair_prot_UmuC-like_N.
    IPR025188. DUF4113.
    IPR024728. PolY_HhH_motif.
    [Graphical view]
    PfamiPF13438. DUF4113. 1 hit.
    PF00817. IMS. 1 hit.
    PF11799. IMS_C. 1 hit.
    PF11798. IMS_HHH. 1 hit.
    [Graphical view]
    SUPFAMiSSF100879. SSF100879. 1 hit.
    PROSITEiPS50173. UMUC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04152-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFALCDVNAF YASCETVFRP DLWGKPVVVL SNNDGCVIAR NAEAKALGVK    50
    MGDPWFKQKD LFRRCGVVCF SSNYELYADM SNRVMSTLEE LSPRVEIYSI 100
    DEAFCDLTGV RNCRDLTDFG REIRATVLQR THLTVGVGIA QTKTLAKLAN 150
    HAAKKWQRQT GGVVDLSNLE RQRKLMSALP VDDVWGIGRR ISKKLDAMGI 200
    KTVLDLADTD IRFIRKHFNV VLERTVRELR GEPCLQLEEF APTKQEIICS 250
    RSFGERITDY PSMRQAICSY AARAAEKLRS EHQYCRFIST FIKTSPFALN 300
    EPYYGNSASV KLLTPTQDSR DIINAATRSL DAIWQAGHRY QKAGVMLGDF 350
    FSQGVAQLNL FDDNAPRPGS EQLMTVMDTL NAKEGRGTLY FAGQGIQQQW 400
    QMKRAMLSPR YTTRSSDLLR VK 422
    Length:422
    Mass (Da):47,680
    Last modified:November 1, 1997 - v2
    Checksum:i0F1855D802A3F5F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti152 – 1521A → E in AAA24729. (PubMed:2989817)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10107 Genomic DNA. Translation: AAA24729.1.
    M13387 Genomic DNA. Translation: AAA98074.1.
    U00096 Genomic DNA. Translation: AAC74268.1.
    AP009048 Genomic DNA. Translation: BAA36031.1.
    PIRiE64864. ZWECC.
    RefSeqiNP_415702.1. NC_000913.3.
    YP_489451.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74268; AAC74268; b1184.
    BAA36031; BAA36031; BAA36031.
    GeneIDi12932822.
    946359.
    KEGGiecj:Y75_p1156.
    eco:b1184.
    PATRICi32117614. VBIEscCol129921_1229.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10107 Genomic DNA. Translation: AAA24729.1 .
    M13387 Genomic DNA. Translation: AAA98074.1 .
    U00096 Genomic DNA. Translation: AAC74268.1 .
    AP009048 Genomic DNA. Translation: BAA36031.1 .
    PIRi E64864. ZWECC.
    RefSeqi NP_415702.1. NC_000913.3.
    YP_489451.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P04152.
    SMRi P04152. Positions 1-351.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11090N.
    IntActi P04152. 9 interactions.
    MINTi MINT-1516814.
    STRINGi 511145.b1184.

    Proteomic databases

    PRIDEi P04152.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74268 ; AAC74268 ; b1184 .
    BAA36031 ; BAA36031 ; BAA36031 .
    GeneIDi 12932822.
    946359.
    KEGGi ecj:Y75_p1156.
    eco:b1184.
    PATRICi 32117614. VBIEscCol129921_1229.

    Organism-specific databases

    EchoBASEi EB1049.
    EcoGenei EG11056. umuC.

    Phylogenomic databases

    eggNOGi COG0389.
    HOGENOMi HOG000252172.
    KOi K03502.
    OMAi PMGAPYF.
    OrthoDBi EOG6CZQNH.
    PhylomeDBi P04152.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11056-MONOMER.
    ECOL316407:JW1173-MONOMER.
    MetaCyc:EG11056-MONOMER.

    Miscellaneous databases

    PROi P04152.

    Gene expression databases

    Genevestigatori P04152.

    Family and domain databases

    Gene3Di 3.30.1490.100. 1 hit.
    InterProi IPR017961. DNA_pol_Y-fam_little_finger.
    IPR001126. DNA_repair_prot_UmuC-like.
    IPR017963. DNA_repair_prot_UmuC-like_N.
    IPR025188. DUF4113.
    IPR024728. PolY_HhH_motif.
    [Graphical view ]
    Pfami PF13438. DUF4113. 1 hit.
    PF00817. IMS. 1 hit.
    PF11799. IMS_C. 1 hit.
    PF11798. IMS_HHH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF100879. SSF100879. 1 hit.
    PROSITEi PS50173. UMUC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli."
      Kitagawa Y., Akaboshi E., Shinagawa H., Horii T., Ogawa H., Kato T.
      Proc. Natl. Acad. Sci. U.S.A. 82:4336-4340(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology."
      Perry K.L., Elledge S.J., Mitchell B.B., Marsh L., Walker G.C.
      Proc. Natl. Acad. Sci. U.S.A. 82:4331-4335(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage."
      Koch W.H., Ennis D.G., Levine A.S., Woodgate R.
      Mol. Gen. Genet. 233:443-448(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.

    Entry informationi

    Entry nameiUMUC_ECOLI
    AccessioniPrimary (citable) accession number: P04152
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3