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Protein

Granulocyte-macrophage colony-stimulating factor

Gene

CSF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164400-MONOMER.
ReactomeiR-HSA-114604. GPVI-mediated activation cascade.
R-HSA-392451. G beta:gamma signalling through PI3Kgamma.
R-HSA-512988. Interleukin-3, 5 and GM-CSF signaling.
R-HSA-5673001. RAF/MAP kinase cascade.
R-HSA-912526. Interleukin receptor SHC signaling.
SignaLinkiP04141.
SIGNORiP04141.

Names & Taxonomyi

Protein namesi
Recommended name:
Granulocyte-macrophage colony-stimulating factor
Short name:
GM-CSF
Alternative name(s):
Colony-stimulating factor
Short name:
CSF
Molgramostin
Sargramostim
Gene namesi
Name:CSF2
Synonyms:GMCSF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:2434. CSF2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Organism-specific databases

DisGeNETi1437.
OpenTargetsiENSG00000164400.
PharmGKBiPA26937.

Polymorphism and mutation databases

BioMutaiCSF2.
DMDMi117561.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Add BLAST17
ChainiPRO_000000586518 – 144Granulocyte-macrophage colony-stimulating factorAdd BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi22O-linked (GalNAc...)1 Publication1
Glycosylationi24O-linked (GalNAc...)1 Publication1
Glycosylationi26O-linked (GalNAc...)1 Publication1
Glycosylationi27O-linked (GalNAc...); partial1 Publication1
Glycosylationi44N-linked (GlcNAc...)1
Glycosylationi54N-linked (GlcNAc...)1
Disulfide bondi71 ↔ 1131 Publication
Disulfide bondi105 ↔ 1381 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP04141.
PRIDEiP04141.

PTM databases

iPTMnetiP04141.
PhosphoSitePlusiP04141.

Miscellaneous databases

PMAP-CutDBP04141.

Expressioni

Gene expression databases

BgeeiENSG00000164400.
CleanExiHS_CSF2.
GenevisibleiP04141. HS.

Interactioni

Subunit structurei

Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CSF2RBP329272EBI-1809826,EBI-1809771
MEOX2A4D1273EBI-1809826,EBI-10172134

GO - Molecular functioni

  • granulocyte macrophage colony-stimulating factor receptor binding Source: ProtInc

Protein-protein interaction databases

BioGridi107824. 11 interactors.
DIPiDIP-386N.
IntActiP04141. 3 interactors.
STRINGi9606.ENSP00000296871.

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni25 – 27Combined sources3
Turni29 – 31Combined sources3
Helixi32 – 45Combined sources14
Helixi50 – 53Combined sources4
Beta strandi56 – 60Combined sources5
Beta strandi66 – 68Combined sources3
Helixi72 – 80Combined sources9
Helixi88 – 90Combined sources3
Helixi91 – 104Combined sources14
Beta strandi115 – 119Combined sources5
Helixi120 – 133Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CSGX-ray2.70A/B18-144[»]
2GMFX-ray2.40A/B18-144[»]
4NKQX-ray3.30B18-144[»]
4RS1X-ray2.68A31-142[»]
5C7XX-ray2.95A/B18-144[»]
5D70X-ray2.06A18-144[»]
5D71X-ray2.25A1-127[»]
5D72X-ray2.60A/B1-127[»]
ProteinModelPortaliP04141.
SMRiP04141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04141.

Family & Domainsi

Sequence similaritiesi

Belongs to the GM-CSF family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JBQ7. Eukaryota.
ENOG411164J. LUCA.
GeneTreeiENSGT00390000013425.
HOGENOMiHOG000037940.
HOVERGENiHBG000532.
InParanoidiP04141.
KOiK05427.
OMAiTCLQTRL.
OrthoDBiEOG091G0XFL.
PhylomeDBiP04141.
TreeFamiTF338611.

Family and domain databases

CDDicd00040. CSF2. 1 hit.
Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000773. GM_colony-stim-fac.
[Graphical view]
PANTHERiPTHR10059. PTHR10059. 1 hit.
PfamiPF01109. GM_CSF. 1 hit.
[Graphical view]
PRINTSiPR00693. GMCSFACTOR.
ProDomiPD007349. GM_colony-stim-fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00040. CSF2. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00702. GM_CSF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLQSLLLLG TVACSISAPA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA
60 70 80 90 100
AEMNETVEVI SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH
110 120 130 140
YKQHCPPTPE TSCATQIITF ESFKENLKDF LLVIPFDCWE PVQE
Length:144
Mass (Da):16,295
Last modified:November 1, 1986 - v1
Checksum:i75D1E50506BCA7A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96M → K in AAM44054 (Ref. 9) Curated1
Sequence conflicti123F → L in AAM44054 (Ref. 9) Curated1

Polymorphismi

Variant Ile-117 may be a risk factor for atopic asthma.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_013089115T → I.1 PublicationCorresponds to variant rs2069640dbSNPEnsembl.1
Natural variantiVAR_001975117I → T.3 PublicationsCorresponds to variant rs25882dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13207 Genomic DNA. Translation: AAA98768.1.
M11734 mRNA. Translation: AAA52122.1.
M11220 mRNA. Translation: AAA52578.1.
X03021 Genomic DNA. Translation: CAA26822.1.
M10663 mRNA. Translation: AAA52121.1.
AF373868 Genomic DNA. Translation: AAK51563.1.
AC004511 Genomic DNA. Translation: AAC08707.1.
BC108724 mRNA. Translation: AAI08725.1.
BC113924 mRNA. Translation: AAI13925.1.
BC113999 mRNA. Translation: AAI14000.1.
AF510855 mRNA. Translation: AAM44054.1.
CCDSiCCDS4150.1.
PIRiC24636. FQHUGM.
RefSeqiNP_000749.2. NM_000758.3.
UniGeneiHs.1349.

Genome annotation databases

EnsembliENST00000296871; ENSP00000296871; ENSG00000164400.
GeneIDi1437.
KEGGihsa:1437.
UCSCiuc003kwf.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Leukine

Clinical information on Leukine

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13207 Genomic DNA. Translation: AAA98768.1.
M11734 mRNA. Translation: AAA52122.1.
M11220 mRNA. Translation: AAA52578.1.
X03021 Genomic DNA. Translation: CAA26822.1.
M10663 mRNA. Translation: AAA52121.1.
AF373868 Genomic DNA. Translation: AAK51563.1.
AC004511 Genomic DNA. Translation: AAC08707.1.
BC108724 mRNA. Translation: AAI08725.1.
BC113924 mRNA. Translation: AAI13925.1.
BC113999 mRNA. Translation: AAI14000.1.
AF510855 mRNA. Translation: AAM44054.1.
CCDSiCCDS4150.1.
PIRiC24636. FQHUGM.
RefSeqiNP_000749.2. NM_000758.3.
UniGeneiHs.1349.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CSGX-ray2.70A/B18-144[»]
2GMFX-ray2.40A/B18-144[»]
4NKQX-ray3.30B18-144[»]
4RS1X-ray2.68A31-142[»]
5C7XX-ray2.95A/B18-144[»]
5D70X-ray2.06A18-144[»]
5D71X-ray2.25A1-127[»]
5D72X-ray2.60A/B1-127[»]
ProteinModelPortaliP04141.
SMRiP04141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107824. 11 interactors.
DIPiDIP-386N.
IntActiP04141. 3 interactors.
STRINGi9606.ENSP00000296871.

PTM databases

iPTMnetiP04141.
PhosphoSitePlusiP04141.

Polymorphism and mutation databases

BioMutaiCSF2.
DMDMi117561.

Proteomic databases

PaxDbiP04141.
PRIDEiP04141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296871; ENSP00000296871; ENSG00000164400.
GeneIDi1437.
KEGGihsa:1437.
UCSCiuc003kwf.5. human.

Organism-specific databases

CTDi1437.
DisGeNETi1437.
GeneCardsiCSF2.
HGNCiHGNC:2434. CSF2.
MIMi138960. gene.
neXtProtiNX_P04141.
OpenTargetsiENSG00000164400.
PharmGKBiPA26937.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410JBQ7. Eukaryota.
ENOG411164J. LUCA.
GeneTreeiENSGT00390000013425.
HOGENOMiHOG000037940.
HOVERGENiHBG000532.
InParanoidiP04141.
KOiK05427.
OMAiTCLQTRL.
OrthoDBiEOG091G0XFL.
PhylomeDBiP04141.
TreeFamiTF338611.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164400-MONOMER.
ReactomeiR-HSA-114604. GPVI-mediated activation cascade.
R-HSA-392451. G beta:gamma signalling through PI3Kgamma.
R-HSA-512988. Interleukin-3, 5 and GM-CSF signaling.
R-HSA-5673001. RAF/MAP kinase cascade.
R-HSA-912526. Interleukin receptor SHC signaling.
SignaLinkiP04141.
SIGNORiP04141.

Miscellaneous databases

EvolutionaryTraceiP04141.
GeneWikiiGranulocyte_macrophage_colony-stimulating_factor.
GenomeRNAii1437.
PMAP-CutDBP04141.
PROiP04141.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164400.
CleanExiHS_CSF2.
GenevisibleiP04141. HS.

Family and domain databases

CDDicd00040. CSF2. 1 hit.
Gene3Di1.20.1250.10. 1 hit.
InterProiIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000773. GM_colony-stim-fac.
[Graphical view]
PANTHERiPTHR10059. PTHR10059. 1 hit.
PfamiPF01109. GM_CSF. 1 hit.
[Graphical view]
PRINTSiPR00693. GMCSFACTOR.
ProDomiPD007349. GM_colony-stim-fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00040. CSF2. 1 hit.
[Graphical view]
SUPFAMiSSF47266. SSF47266. 1 hit.
PROSITEiPS00702. GM_CSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSF2_HUMAN
AccessioniPrimary (citable) accession number: P04141
Secondary accession number(s): Q14CE8, Q2VPI8, Q8NFI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: November 2, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.