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Protein

Pap fimbrial major pilin protein

Gene

papA

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Polymerizes to form the thick (6.8 nm in diameter) rod of the pilus (also called fimbria). The rod is a right-handed helical cylinder with 3.28 PapA subunits per turn. Pili are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, and enable bacteria to colonize the epithelium of specific host organs.

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Pap fimbrial major pilin protein
Short name:
Pap pili
Gene namesi
Name:papA
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Fimbrium, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Chaini23 – 185163Pap fimbrial major pilin proteinPRO_0000009197Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 831 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

DIPiDIP-44594N.
IntActiP04127. 1 interaction.
MINTiMINT-4055086.

Structurei

Secondary structure

1
185
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 408Combined sources
Beta strandi45 – 473Combined sources
Beta strandi54 – 563Combined sources
Helixi62 – 676Combined sources
Beta strandi74 – 8310Combined sources
Beta strandi98 – 1047Combined sources
Beta strandi111 – 1166Combined sources
Beta strandi118 – 1214Combined sources
Beta strandi123 – 1286Combined sources
Beta strandi130 – 1334Combined sources
Beta strandi137 – 1404Combined sources
Beta strandi152 – 16110Combined sources
Beta strandi175 – 18410Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UY6X-ray2.50B/C23-185[»]
2UY7X-ray2.60B/D/F/H23-185[»]
5FLUelectron microscopy3.80A/B/C/D/E/F/G/H/I/J/K/L/M23-185[»]
ProteinModelPortaliP04127.
SMRiP04127. Positions 30-185.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04127.

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial protein family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1090. 1 hit.
InterProiIPR008966. Adhesion_dom.
IPR000259. Adhesion_dom_fimbrial.
[Graphical view]
PfamiPF00419. Fimbrial. 1 hit.
[Graphical view]
SUPFAMiSSF49401. SSF49401. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04127-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKSVIAGAV AMAVVSFGVN NAAPTIPQGQ GKVTFNGTVV DAPCSISQKS
60 70 80 90 100
ADQSIDFGQL SKSFLEAGGV SKPMDLDIEL VNCDITAFKG GNGAKKGTVK
110 120 130 140 150
LAFTGPIVNG HSDELDTNGG TGTAIVVQGA GKNVVFDGSE GDANTLKDGE
160 170 180
NVLHYTAVVK KSSAVGAAVT EGAFSAVANF NLTYQ
Length:185
Mass (Da):18,686
Last modified:November 1, 1986 - v1
Checksum:i93DB4FFDA211C671
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03391 Genomic DNA. Translation: CAA27126.1.
X61239 Genomic DNA. Translation: CAA43562.1.
PIRiA23221. YQECPP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03391 Genomic DNA. Translation: CAA27126.1.
X61239 Genomic DNA. Translation: CAA43562.1.
PIRiA23221. YQECPP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UY6X-ray2.50B/C23-185[»]
2UY7X-ray2.60B/D/F/H23-185[»]
5FLUelectron microscopy3.80A/B/C/D/E/F/G/H/I/J/K/L/M23-185[»]
ProteinModelPortaliP04127.
SMRiP04127. Positions 30-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-44594N.
IntActiP04127. 1 interaction.
MINTiMINT-4055086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP04127.

Family and domain databases

Gene3Di2.60.40.1090. 1 hit.
InterProiIPR008966. Adhesion_dom.
IPR000259. Adhesion_dom_fimbrial.
[Graphical view]
PfamiPF00419. Fimbrial. 1 hit.
[Graphical view]
SUPFAMiSSF49401. SSF49401. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the papA gene encoding the Pap pilus subunit of human uropathogenic Escherichia coli."
    Baga M., Normark S., Hardy J., O'Hanley P., Lark D., Olsson O., Schoolnik G., Falkow S.
    J. Bacteriol. 157:330-333(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700336 / J96.
  2. "Horizontal gene transfer of the Escherichia coli pap and prs pili operons as a mechanism for the development of tissue-specific adhesive properties."
    Marklund B.-I., Tennent J.M., Garcia E., Hamers A., Baga M., Lindberg F., Gaastra W., Normark S.
    Mol. Microbiol. 6:2225-2242(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700336 / J96.
  3. "Pilus biogenesis via the chaperone/usher pathway: an integration of structure and function."
    Hung D.L., Hultgren S.J.
    J. Struct. Biol. 124:201-220(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  4. "Crystal structure of the P pilus rod subunit PapA."
    Verger D., Bullitt E., Hultgren S.J., Waksman G.
    PLoS Pathog. 3:674-682(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 23-185 IN COMPLEX WITH PAPD, DISULFIDE BOND.

Entry informationi

Entry nameiPAPA_ECOLX
AccessioniPrimary (citable) accession number: P04127
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: April 13, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Strains of E.coli that cause infection of the human urinary tract produce pap-pili which are hair-like appendages consisting of about 1000 helically arranged subunits of the protein PapA. These pili mediate binding to digalactoside-containing glycolipids present on the epithelial cells which line the urinary tract.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.