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P04094

- PENK_RAT

UniProt

P04094 - PENK_RAT

Protein

Proenkephalin-A

Gene

Penk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1986)
      Previous versions | rss
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    Functioni

    Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(239-260) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.1 Publication

    GO - Biological processi

    1. aggressive behavior Source: Ensembl
    2. behavioral fear response Source: Ensembl
    3. locomotory behavior Source: Ensembl
    4. neuropeptide signaling pathway Source: UniProtKB-KW
    5. sensory perception of pain Source: Ensembl
    6. startle response Source: Ensembl

    Keywords - Molecular functioni

    Endorphin, Neuropeptide, Opioid peptide

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proenkephalin-A
    Cleaved into the following 8 chains:
    Gene namesi
    Name:Penk
    Synonyms:Penk-rs, Penk1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi68946. Penk.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Peptidei25 – 9773SynenkephalinPRO_0000008281Add
    BLAST
    Peptidei100 – 1045Met-enkephalinPRO_0000008282
    Peptidei107 – 1115Met-enkephalinPRO_0000008283
    Peptidei114 – 13320PENK(114-133)PRO_0000008284Add
    BLAST
    Peptidei136 – 1405Met-enkephalinPRO_0000008285
    Peptidei143 – 18543PENK(143-185)PRO_0000008286Add
    BLAST
    Peptidei188 – 1958Met-enkephalin-Arg-Gly-LeuPRO_0000008287
    Propeptidei198 – 20912PRO_0000008288Add
    BLAST
    Peptidei212 – 2165Met-enkephalinPRO_0000008289
    Propeptidei219 – 22911PRO_0000008290Add
    BLAST
    Peptidei232 – 2365Leu-enkephalinPRO_0000008291
    Peptidei239 – 26022PENK(239-260)PRO_0000008292Add
    BLAST
    Peptidei263 – 2697Met-enkephalin-Arg-PhePRO_0000008293

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 48By similarity
    Disulfide bondi30 ↔ 52By similarity
    Disulfide bondi33 ↔ 65By similarity

    Post-translational modificationi

    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    PTM databases

    PhosphoSiteiP04094.

    Miscellaneous databases

    PMAP-CutDBP04094.

    Expressioni

    Tissue specificityi

    Expressed in brain, heart and testis.

    Gene expression databases

    GenevestigatoriP04094.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000011892.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40630.
    GeneTreeiENSGT00530000063761.
    HOGENOMiHOG000013003.
    HOVERGENiHBG000063.
    InParanoidiQ53X05.
    OMAiGGFMKKD.
    OrthoDBiEOG75TMCR.
    PhylomeDBiP04094.
    TreeFamiTF332620.

    Family and domain databases

    InterProiIPR006024. Opioid_neupept.
    IPR000703. Proenkphlin_A.
    [Graphical view]
    PANTHERiPTHR11438. PTHR11438. 1 hit.
    PTHR11438:SF3. PTHR11438:SF3. 1 hit.
    PfamiPF01160. Opiods_neuropep. 1 hit.
    [Graphical view]
    PRINTSiPR01028. OPIOIDPRCRSR.
    PR01029. PENKAPRCRSR.
    PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04094-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQFLRLCIW LLALGSCLLA TVQADCSQDC AKCSYRLVRP GDINFLACTL    50
    ECEGQLPSFK IWETCKDLLQ VSKPEFPWDN IDMYKDSSKQ DESHLLAKKY 100
    GGFMKRYGGF MKKMDELYPV EPEEEANGGE ILAKRYGGFM KKDADEGDTL 150
    ANSSDLLKEL LGTGDNRAKD SHQQESTNND EDSTSKRYGG FMRGLKRSPQ 200
    LEDEAKELQK RYGGFMRRVG RPEWWMDYQK RYGGFLKRFA ESLPSDEEGE 250
    SYSKEVPEME KRYGGFMRF 269
    Length:269
    Mass (Da):30,932
    Last modified:November 1, 1986 - v1
    Checksum:i2BF0EA81A1D59692
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121L → V in AAA60734. (PubMed:6094550)Curated
    Sequence conflicti12 – 121L → V in CAA68804. (PubMed:2707437)Curated

    Mass spectrometryi

    Molecular mass is 2203.971 Da from positions 114 - 133. Determined by ESI. 1 Publication
    Molecular mass is 4592.8 Da from positions 143 - 185. Determined by ESI. 1 Publication
    Molecular mass is 2504.027 Da from positions 239 - 260. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02807 Genomic DNA. Translation: AAA60734.1.
    M28263 mRNA. Translation: AAA41115.1.
    Y07503 mRNA. Translation: CAA68804.1.
    S49491 mRNA. Translation: AAB24022.1.
    BC083563 mRNA. Translation: AAH83563.1.
    PIRiA92464. EQRTA.
    RefSeqiNP_058835.1. NM_017139.1.
    XP_006237897.1. XM_006237835.1.
    UniGeneiRn.10015.

    Genome annotation databases

    EnsembliENSRNOT00000011892; ENSRNOP00000011892; ENSRNOG00000008943.
    GeneIDi29237.
    KEGGirno:29237.
    UCSCiRGD:68946. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02807 Genomic DNA. Translation: AAA60734.1 .
    M28263 mRNA. Translation: AAA41115.1 .
    Y07503 mRNA. Translation: CAA68804.1 .
    S49491 mRNA. Translation: AAB24022.1 .
    BC083563 mRNA. Translation: AAH83563.1 .
    PIRi A92464. EQRTA.
    RefSeqi NP_058835.1. NM_017139.1.
    XP_006237897.1. XM_006237835.1.
    UniGenei Rn.10015.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000011892.

    PTM databases

    PhosphoSitei P04094.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000011892 ; ENSRNOP00000011892 ; ENSRNOG00000008943 .
    GeneIDi 29237.
    KEGGi rno:29237.
    UCSCi RGD:68946. rat.

    Organism-specific databases

    CTDi 5179.
    RGDi 68946. Penk.

    Phylogenomic databases

    eggNOGi NOG40630.
    GeneTreei ENSGT00530000063761.
    HOGENOMi HOG000013003.
    HOVERGENi HBG000063.
    InParanoidi Q53X05.
    OMAi GGFMKKD.
    OrthoDBi EOG75TMCR.
    PhylomeDBi P04094.
    TreeFami TF332620.

    Miscellaneous databases

    NextBioi 608502.
    PMAP-CutDB P04094.
    PROi P04094.

    Gene expression databases

    Genevestigatori P04094.

    Family and domain databases

    InterProi IPR006024. Opioid_neupept.
    IPR000703. Proenkphlin_A.
    [Graphical view ]
    PANTHERi PTHR11438. PTHR11438. 1 hit.
    PTHR11438:SF3. PTHR11438:SF3. 1 hit.
    Pfami PF01160. Opiods_neuropep. 1 hit.
    [Graphical view ]
    PRINTSi PR01028. OPIOIDPRCRSR.
    PR01029. PENKAPRCRSR.
    PROSITEi PS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat brain preproenkephalin mRNA. cDNA cloning, primary structure, and distribution in the central nervous system."
      Yoshikawa K., Williams C., Sabol S.L.
      J. Biol. Chem. 259:14301-14308(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Fischer 344.
      Tissue: Brain.
    2. "Isolation and characterization of the rat proenkephalin gene."
      Rosen H., Douglass J., Herbert E.
      J. Biol. Chem. 259:14309-14313(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Fischer 344.
    3. "Molecular cloning and sequence determination of rat preproenkephalin cDNA: sensitive probe for studying transcriptional changes in rat tissues."
      Howells R.D., Kilpatrick D.L., Bhatt R., Monahan J.J., Poonian M., Udenfriend S.
      Proc. Natl. Acad. Sci. U.S.A. 81:7651-7655(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Fischer 344.
      Tissue: Brain.
    4. "Proenkephalin biosynthesis in the rat."
      Howells R.D.
      NIDA Res. Monogr. 70:43-65(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    5. "A new species of enkephalin precursor mRNA with a distinct 5'-untranslated region in haploid germ cells."
      Yoshikawa K., Maruyama K., Aizawa T., Yamamoto A.
      FEBS Lett. 246:193-196(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Testis.
    6. "Molecular cloning, sequence analysis and translation of proenkephalin mRNA from rat heart."
      Rao S.M., Howells R.D.
      Regul. Pept. 40:397-408(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    8. "Discovering new bioactive neuropeptides in the striatum secretome using in vivo microdialysis and versatile proteomics."
      Bernay B., Gaillard M.-C., Guryca V., Emadali A., Kuhn L., Bertrand A., Detraz I., Carcenac C., Savasta M., Brouillet E., Garin J., Elalouf J.-M.
      Mol. Cell. Proteomics 8:946-958(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 114-133; 143-185 AND 239-260, FUNCTION, MASS SPECTROMETRY.
      Strain: Wistar.
      Tissue: Corpus striatum.

    Entry informationi

    Entry nameiPENK_RAT
    AccessioniPrimary (citable) accession number: P04094
    Secondary accession number(s): Q53X05
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1986
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3