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Protein

Proenkephalin-A

Gene

Penk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(239-260) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.1 Publication

GO - Molecular functioni

GO - Biological processi

  • aggressive behavior Source: Ensembl
  • aging Source: RGD
  • behavioral fear response Source: Ensembl
  • cellular response to cAMP Source: RGD
  • cellular response to oxidative stress Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • cellular response to virus Source: RGD
  • cellular response to vitamin D Source: RGD
  • chemical synaptic transmission Source: GO_Central
  • general adaptation syndrome, behavioral process Source: RGD
  • glial cell proliferation Source: RGD
  • locomotory exploration behavior Source: RGD
  • neuropeptide signaling pathway Source: GO_Central
  • osteoblast differentiation Source: RGD
  • positive regulation of behavioral fear response Source: RGD
  • response to calcium ion Source: RGD
  • response to epinephrine Source: RGD
  • response to estradiol Source: RGD
  • response to ethanol Source: RGD
  • response to hypoxia Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to morphine Source: RGD
  • response to nicotine Source: RGD
  • response to radiation Source: RGD
  • response to toxic substance Source: RGD
  • sensory perception of pain Source: Ensembl
  • startle response Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Endorphin, Neuropeptide, Opioid peptide

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Proenkephalin-A
Cleaved into the following 8 chains:
Gene namesi
Name:Penk
Synonyms:Penk-rs, Penk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi68946. Penk.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • axon terminus Source: RGD
  • cell body fiber Source: RGD
  • dendrite Source: RGD
  • extracellular region Source: GO_Central
  • neuronal cell body Source: RGD
  • perikaryon Source: RGD
  • plasma membrane Source: GO_Central
  • symmetric synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PeptideiPRO_000000828125 – 97SynenkephalinAdd BLAST73
PeptideiPRO_0000008282100 – 104Met-enkephalin5
PeptideiPRO_0000008283107 – 111Met-enkephalin5
PeptideiPRO_0000008284114 – 133PENK(114-133)Add BLAST20
PeptideiPRO_0000008285136 – 140Met-enkephalin5
PeptideiPRO_0000008286143 – 185PENK(143-185)Add BLAST43
PeptideiPRO_0000008287188 – 195Met-enkephalin-Arg-Gly-Leu8
PropeptideiPRO_0000008288198 – 209Add BLAST12
PeptideiPRO_0000008289212 – 216Met-enkephalin5
PropeptideiPRO_0000008290219 – 229Add BLAST11
PeptideiPRO_0000008291232 – 236Leu-enkephalin5
PeptideiPRO_0000008292239 – 260PENK(239-260)Add BLAST22
PeptideiPRO_0000008293263 – 269Met-enkephalin-Arg-Phe7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 48By similarity
Disulfide bondi30 ↔ 52By similarity
Disulfide bondi33 ↔ 65By similarity
Modified residuei253PhosphoserineCombined sources1

Post-translational modificationi

The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP04094.

PTM databases

iPTMnetiP04094.
PhosphoSitePlusiP04094.

Miscellaneous databases

PMAP-CutDBP04094.

Expressioni

Tissue specificityi

Expressed in brain, heart and testis.

Gene expression databases

BgeeiENSRNOG00000008943.
GenevisibleiP04094. RN.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011892.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJUY. Eukaryota.
ENOG4110ZYD. LUCA.
GeneTreeiENSGT00530000063761.
HOGENOMiHOG000013003.
HOVERGENiHBG000063.
InParanoidiP04094.
KOiK18832.
OMAiFMKKMDE.
OrthoDBiEOG091G0HV8.
PhylomeDBiP04094.
TreeFamiTF332620.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04094-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFLRLCIW LLALGSCLLA TVQADCSQDC AKCSYRLVRP GDINFLACTL
60 70 80 90 100
ECEGQLPSFK IWETCKDLLQ VSKPEFPWDN IDMYKDSSKQ DESHLLAKKY
110 120 130 140 150
GGFMKRYGGF MKKMDELYPV EPEEEANGGE ILAKRYGGFM KKDADEGDTL
160 170 180 190 200
ANSSDLLKEL LGTGDNRAKD SHQQESTNND EDSTSKRYGG FMRGLKRSPQ
210 220 230 240 250
LEDEAKELQK RYGGFMRRVG RPEWWMDYQK RYGGFLKRFA ESLPSDEEGE
260
SYSKEVPEME KRYGGFMRF
Length:269
Mass (Da):30,932
Last modified:November 1, 1986 - v1
Checksum:i2BF0EA81A1D59692
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12L → V in AAA60734 (PubMed:6094550).Curated1
Sequence conflicti12L → V in CAA68804 (PubMed:2707437).Curated1

Mass spectrometryi

Molecular mass is 2203.971 Da from positions 114 - 133. Determined by ESI. 1 Publication
Molecular mass is 4592.8 Da from positions 143 - 185. Determined by ESI. 1 Publication
Molecular mass is 2504.027 Da from positions 239 - 260. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02807 Genomic DNA. Translation: AAA60734.1.
M28263 mRNA. Translation: AAA41115.1.
Y07503 mRNA. Translation: CAA68804.1.
S49491 mRNA. Translation: AAB24022.1.
BC083563 mRNA. Translation: AAH83563.1.
PIRiA92464. EQRTA.
RefSeqiNP_058835.1. NM_017139.1.
XP_006237897.1. XM_006237835.1.
UniGeneiRn.10015.

Genome annotation databases

EnsembliENSRNOT00000011892; ENSRNOP00000011892; ENSRNOG00000008943.
ENSRNOT00000089318; ENSRNOP00000074581; ENSRNOG00000008943.
GeneIDi29237.
KEGGirno:29237.
UCSCiRGD:68946. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02807 Genomic DNA. Translation: AAA60734.1.
M28263 mRNA. Translation: AAA41115.1.
Y07503 mRNA. Translation: CAA68804.1.
S49491 mRNA. Translation: AAB24022.1.
BC083563 mRNA. Translation: AAH83563.1.
PIRiA92464. EQRTA.
RefSeqiNP_058835.1. NM_017139.1.
XP_006237897.1. XM_006237835.1.
UniGeneiRn.10015.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011892.

PTM databases

iPTMnetiP04094.
PhosphoSitePlusiP04094.

Proteomic databases

PaxDbiP04094.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011892; ENSRNOP00000011892; ENSRNOG00000008943.
ENSRNOT00000089318; ENSRNOP00000074581; ENSRNOG00000008943.
GeneIDi29237.
KEGGirno:29237.
UCSCiRGD:68946. rat.

Organism-specific databases

CTDi5179.
RGDi68946. Penk.

Phylogenomic databases

eggNOGiENOG410IJUY. Eukaryota.
ENOG4110ZYD. LUCA.
GeneTreeiENSGT00530000063761.
HOGENOMiHOG000013003.
HOVERGENiHBG000063.
InParanoidiP04094.
KOiK18832.
OMAiFMKKMDE.
OrthoDBiEOG091G0HV8.
PhylomeDBiP04094.
TreeFamiTF332620.

Enzyme and pathway databases

ReactomeiR-RNO-375276. Peptide ligand-binding receptors.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

PMAP-CutDBP04094.
PROiP04094.

Gene expression databases

BgeeiENSRNOG00000008943.
GenevisibleiP04094. RN.

Family and domain databases

InterProiIPR006024. Opioid_neupept.
IPR000703. Proenkphlin_A.
[Graphical view]
PANTHERiPTHR11438. PTHR11438. 1 hit.
PTHR11438:SF3. PTHR11438:SF3. 1 hit.
PfamiPF01160. Opiods_neuropep. 1 hit.
[Graphical view]
PRINTSiPR01028. OPIOIDPRCRSR.
PR01029. PENKAPRCRSR.
PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPENK_RAT
AccessioniPrimary (citable) accession number: P04094
Secondary accession number(s): Q53X05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.