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P04090

- REL2_HUMAN

UniProt

P04090 - REL2_HUMAN

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Protein
Prorelaxin H2
Gene
RLN2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

GO - Biological processi

  1. female pregnancy Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiREACT_19327. G alpha (s) signalling events.
REACT_21314. Relaxin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Prorelaxin H2
Cleaved into the following 2 chains:
Gene namesi
Name:RLN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:10027. RLN2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34400.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24242 Publications
Add
BLAST
Peptidei25 – 5329Relaxin B chain1 Publication
PRO_0000016079Add
BLAST
Propeptidei56 – 157102Connecting peptide
PRO_0000016080Add
BLAST
Peptidei162 – 18524Relaxin A chain
PRO_0000016081Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 172Interchain (between B and A chains)
Disulfide bondi47 ↔ 185Interchain (between B and A chains)
Modified residuei162 – 1621Pyrrolidone carboxylic acid1 Publication
Disulfide bondi171 ↔ 1761 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP04090.
PRIDEiP04090.

PTM databases

PhosphoSiteiP04090.

Expressioni

Tissue specificityi

Isoform 1 is expressed in the ovary during pregnancy. Also expressed in placenta, decidua and prostate. Isoform 2 is relatively abundant in placenta. It is in much lower abundance in the prostate gland. Not detected in the ovary.2 Publications

Gene expression databases

BgeeiP04090.
CleanExiHS_RLN2.
GenevestigatoriP04090.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Protein-protein interaction databases

STRINGi9606.ENSP00000371040.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi27 – 293
Helixi36 – 4914
Helixi163 – 17311
Helixi178 – 1825

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
6RLXX-ray1.50A/C163-185[»]
B/D26-53[»]
ProteinModelPortaliP04090.
SMRiP04090. Positions 26-52.

Miscellaneous databases

EvolutionaryTraceiP04090.

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40989.
HOGENOMiHOG000236342.
HOVERGENiHBG003234.
InParanoidiP04090.
KOiK05255.
OMAiLSEKCCQ.
OrthoDBiEOG7QZGC5.
PhylomeDBiP04090.
TreeFamiTF333404.

Family and domain databases

Gene3Di1.10.100.10. 2 hits.
InterProiIPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
IPR022421. Relaxin.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00276. INSULINFAMLY.
PR02004. RELAXIN.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P04090-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPRLFFFHLL GVCLLLNQFS RAVADSWMEE VIKLCGRELV RAQIAICGMS    50
TWSKRSLSQE DAPQTPRPVA EIVPSFINKD TETINMMSEF VANLPQELKL 100
TLSEMQPALP QLQQHVPVLK DSSLLFEEFK KLIRNRQSEA ADSSPSELKY 150
LGLDTHSRKK RQLYSALANK CCHVGCTKRS LARFC 185
Length:185
Mass (Da):21,043
Last modified:November 1, 1986 - v1
Checksum:iAC73DBDE2090091B
GO
Isoform 2 (identifier: P04090-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     71-117: EIVPSFINKD...ALPQLQQHVP → GDFIQTVSLG...HPSSTKIQKP
     118-185: Missing.

Show »
Length:117
Mass (Da):12,883
Checksum:i087AE9F24582740A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti12 – 121V → F.
Corresponds to variant rs2020050 [ dbSNP | Ensembl ].
VAR_050007
Natural varianti28 – 281M → K.
Corresponds to variant rs618066 [ dbSNP | Ensembl ].
VAR_034444
Natural varianti68 – 681P → Q.
Corresponds to variant rs2273783 [ dbSNP | Ensembl ].
VAR_022124

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei71 – 11747EIVPS…QQHVP → GDFIQTVSLGISPDGGKALR TGSCFTREFLGALSKLCHPS STKIQKP in isoform 2.
VSP_002711Add
BLAST
Alternative sequencei118 – 18568Missing in isoform 2.
VSP_002712Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00948 mRNA. Translation: CAA25460.1.
AL135786 Genomic DNA. Translation: CAC04177.1.
BC126415 mRNA. Translation: AAI26416.1.
BC126419 mRNA. Translation: AAI26420.1.
S83200 Genomic DNA. Translation: AAD14429.1. Sequence problems.
AF104935 Genomic DNA. Translation: AAD21961.1.
CCDSiCCDS6460.1. [P04090-1]
PIRiA05092. A60982.
RefSeqiNP_005050.2. NM_005059.3. [P04090-2]
NP_604390.1. NM_134441.2. [P04090-1]
UniGeneiHs.127032.
Hs.368996.

Genome annotation databases

EnsembliENST00000308420; ENSP00000308018; ENSG00000107014. [P04090-2]
ENST00000381627; ENSP00000371040; ENSG00000107014. [P04090-1]
GeneIDi6019.
KEGGihsa:6019.
UCSCiuc003ziz.2. human. [P04090-2]
uc003zja.2. human. [P04090-1]

Polymorphism databases

DMDMi132281.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00948 mRNA. Translation: CAA25460.1 .
AL135786 Genomic DNA. Translation: CAC04177.1 .
BC126415 mRNA. Translation: AAI26416.1 .
BC126419 mRNA. Translation: AAI26420.1 .
S83200 Genomic DNA. Translation: AAD14429.1 . Sequence problems.
AF104935 Genomic DNA. Translation: AAD21961.1 .
CCDSi CCDS6460.1. [P04090-1 ]
PIRi A05092. A60982.
RefSeqi NP_005050.2. NM_005059.3. [P04090-2 ]
NP_604390.1. NM_134441.2. [P04090-1 ]
UniGenei Hs.127032.
Hs.368996.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
6RLX X-ray 1.50 A/C 163-185 [» ]
B/D 26-53 [» ]
ProteinModelPortali P04090.
SMRi P04090. Positions 26-52.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000371040.

PTM databases

PhosphoSitei P04090.

Polymorphism databases

DMDMi 132281.

Proteomic databases

PaxDbi P04090.
PRIDEi P04090.

Protocols and materials databases

DNASUi 6019.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308420 ; ENSP00000308018 ; ENSG00000107014 . [P04090-2 ]
ENST00000381627 ; ENSP00000371040 ; ENSG00000107014 . [P04090-1 ]
GeneIDi 6019.
KEGGi hsa:6019.
UCSCi uc003ziz.2. human. [P04090-2 ]
uc003zja.2. human. [P04090-1 ]

Organism-specific databases

CTDi 6019.
GeneCardsi GC09M005305.
HGNCi HGNC:10027. RLN2.
MIMi 179740. gene.
neXtProti NX_P04090.
PharmGKBi PA34400.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG40989.
HOGENOMi HOG000236342.
HOVERGENi HBG003234.
InParanoidi P04090.
KOi K05255.
OMAi LSEKCCQ.
OrthoDBi EOG7QZGC5.
PhylomeDBi P04090.
TreeFami TF333404.

Enzyme and pathway databases

Reactomei REACT_19327. G alpha (s) signalling events.
REACT_21314. Relaxin receptors.

Miscellaneous databases

EvolutionaryTracei P04090.
GenomeRNAii 6019.
NextBioi 23483.
PROi P04090.
SOURCEi Search...

Gene expression databases

Bgeei P04090.
CleanExi HS_RLN2.
Genevestigatori P04090.

Family and domain databases

Gene3Di 1.10.100.10. 2 hits.
InterProi IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
IPR022421. Relaxin.
[Graphical view ]
Pfami PF00049. Insulin. 1 hit.
[Graphical view ]
PRINTSi PR00276. INSULINFAMLY.
PR02004. RELAXIN.
SMARTi SM00078. IlGF. 1 hit.
[Graphical view ]
SUPFAMi SSF56994. SSF56994. 2 hits.
PROSITEi PS00262. INSULIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Relaxin gene expression in human ovaries and the predicted structure of a human preprorelaxin by analysis of cDNA clones."
    Hudson P., John M., Crawford R., Haralambidis J., Scanlon D., Gorman J., Tregear G., Shine J., Niall H.
    EMBO J. 3:2333-2339(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "Expression of human relaxin genes: characterization of a novel alternatively-spliced human relaxin mRNA species."
    Gunnersen J.M., Fu P., Roche P.J., Tregear G.W.
    Mol. Cell. Endocrinol. 118:85-94(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-185 (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: Prostate.
  5. "Analysis of the 5'-upstream regions of the human relaxin H1 and H2 genes and their chromosomal localization on chromosome 9p24.1 by radiation hybrid and breakpoint mapping."
    Garibay-Tupas J.L., Csiszar K., Fox M., Povey S., Bryant-Greenwood G.D.
    J. Mol. Endocrinol. 23:355-365(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
  6. "Human seminal relaxin is a product of the same gene as human luteal relaxin."
    Winslow J.W., Shih A., Bourell J.H., Weiss G., Reed B., Stults J.T., Goldsmith L.T.
    Endocrinology 130:2660-2668(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-51.
    Tissue: Semen.
  7. "Structural characterization by mass spectrometry of native and recombinant human relaxin."
    Winslow J.W., Griffin P.R., Rinderknecht E., Vandlen R.L.
    Biomed. Environ. Mass Spectrom. 19:655-664(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-53 AND 162-185 (ISOFORM 1), PYROGLUTAMATE FORMATION AT GLN-162.
  8. "Total synthesis of human relaxin and human relaxin derivatives by solid-phase peptide synthesis and site-directed chain combination."
    Buellesbach E.E., Schwabe C.
    J. Biol. Chem. 266:10754-10761(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SYNTHESIS.
  9. "X-ray structure of human relaxin at 1.5 A. Comparison to insulin and implications for receptor binding determinants."
    Eigenbrot C., Randal M., Quan C., Burnier J., O'Connell L., Rinderknecht E., Kossiakoff A.A.
    J. Mol. Biol. 221:15-21(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Entry informationi

Entry nameiREL2_HUMAN
AccessioniPrimary (citable) accession number: P04090
Secondary accession number(s): A0AVM0
, Q99936, Q9UCX3, Q9UQJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: September 3, 2014
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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