Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P04090

- REL2_HUMAN

UniProt

P04090 - REL2_HUMAN

Protein

Prorelaxin H2

Gene

RLN2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

    GO - Biological processi

    1. female pregnancy Source: ProtInc

    Keywords - Molecular functioni

    Hormone

    Enzyme and pathway databases

    ReactomeiREACT_19327. G alpha (s) signalling events.
    REACT_21314. Relaxin receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prorelaxin H2
    Cleaved into the following 2 chains:
    Gene namesi
    Name:RLN2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:10027. RLN2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34400.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 24241 PublicationAdd
    BLAST
    Peptidei25 – 5329Relaxin B chainPRO_0000016079Add
    BLAST
    Propeptidei56 – 157102Connecting peptidePRO_0000016080Add
    BLAST
    Peptidei162 – 18524Relaxin A chainPRO_0000016081Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi35 ↔ 172Interchain (between B and A chains)
    Disulfide bondi47 ↔ 185Interchain (between B and A chains)
    Modified residuei162 – 1621Pyrrolidone carboxylic acid1 Publication
    Disulfide bondi171 ↔ 1761 Publication

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP04090.
    PRIDEiP04090.

    PTM databases

    PhosphoSiteiP04090.

    Expressioni

    Tissue specificityi

    Isoform 1 is expressed in the ovary during pregnancy. Also expressed in placenta, decidua and prostate. Isoform 2 is relatively abundant in placenta. It is in much lower abundance in the prostate gland. Not detected in the ovary.2 Publications

    Gene expression databases

    BgeeiP04090.
    CleanExiHS_RLN2.
    GenevestigatoriP04090.

    Interactioni

    Subunit structurei

    Heterodimer of a B chain and an A chain linked by two disulfide bonds.

    Protein-protein interaction databases

    STRINGi9606.ENSP00000371040.

    Structurei

    Secondary structure

    1
    185
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi27 – 293
    Helixi36 – 4914
    Helixi163 – 17311
    Helixi178 – 1825

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    6RLXX-ray1.50A/C163-185[»]
    B/D26-53[»]
    ProteinModelPortaliP04090.
    SMRiP04090. Positions 26-52.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04090.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the insulin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40989.
    HOGENOMiHOG000236342.
    HOVERGENiHBG003234.
    InParanoidiP04090.
    KOiK05255.
    OMAiLSEKCCQ.
    OrthoDBiEOG7QZGC5.
    PhylomeDBiP04090.
    TreeFamiTF333404.

    Family and domain databases

    Gene3Di1.10.100.10. 2 hits.
    InterProiIPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    IPR022421. Relaxin.
    [Graphical view]
    PfamiPF00049. Insulin. 1 hit.
    [Graphical view]
    PRINTSiPR00276. INSULINFAMLY.
    PR02004. RELAXIN.
    SMARTiSM00078. IlGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56994. SSF56994. 2 hits.
    PROSITEiPS00262. INSULIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P04090-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPRLFFFHLL GVCLLLNQFS RAVADSWMEE VIKLCGRELV RAQIAICGMS    50
    TWSKRSLSQE DAPQTPRPVA EIVPSFINKD TETINMMSEF VANLPQELKL 100
    TLSEMQPALP QLQQHVPVLK DSSLLFEEFK KLIRNRQSEA ADSSPSELKY 150
    LGLDTHSRKK RQLYSALANK CCHVGCTKRS LARFC 185
    Length:185
    Mass (Da):21,043
    Last modified:November 1, 1986 - v1
    Checksum:iAC73DBDE2090091B
    GO
    Isoform 2 (identifier: P04090-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         71-117: EIVPSFINKD...ALPQLQQHVP → GDFIQTVSLG...HPSSTKIQKP
         118-185: Missing.

    Show »
    Length:117
    Mass (Da):12,883
    Checksum:i087AE9F24582740A
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti12 – 121V → F.
    Corresponds to variant rs2020050 [ dbSNP | Ensembl ].
    VAR_050007
    Natural varianti28 – 281M → K.
    Corresponds to variant rs618066 [ dbSNP | Ensembl ].
    VAR_034444
    Natural varianti68 – 681P → Q.
    Corresponds to variant rs2273783 [ dbSNP | Ensembl ].
    VAR_022124

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei71 – 11747EIVPS…QQHVP → GDFIQTVSLGISPDGGKALR TGSCFTREFLGALSKLCHPS STKIQKP in isoform 2. CuratedVSP_002711Add
    BLAST
    Alternative sequencei118 – 18568Missing in isoform 2. CuratedVSP_002712Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00948 mRNA. Translation: CAA25460.1.
    AL135786 Genomic DNA. Translation: CAC04177.1.
    BC126415 mRNA. Translation: AAI26416.1.
    BC126419 mRNA. Translation: AAI26420.1.
    S83200 Genomic DNA. Translation: AAD14429.1. Sequence problems.
    AF104935 Genomic DNA. Translation: AAD21961.1.
    CCDSiCCDS6460.1. [P04090-1]
    PIRiA05092. A60982.
    RefSeqiNP_005050.2. NM_005059.3. [P04090-2]
    NP_604390.1. NM_134441.2. [P04090-1]
    UniGeneiHs.127032.
    Hs.368996.

    Genome annotation databases

    EnsembliENST00000381627; ENSP00000371040; ENSG00000107014. [P04090-1]
    GeneIDi6019.
    KEGGihsa:6019.
    UCSCiuc003ziz.2. human. [P04090-2]
    uc003zja.2. human. [P04090-1]

    Polymorphism databases

    DMDMi132281.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00948 mRNA. Translation: CAA25460.1 .
    AL135786 Genomic DNA. Translation: CAC04177.1 .
    BC126415 mRNA. Translation: AAI26416.1 .
    BC126419 mRNA. Translation: AAI26420.1 .
    S83200 Genomic DNA. Translation: AAD14429.1 . Sequence problems.
    AF104935 Genomic DNA. Translation: AAD21961.1 .
    CCDSi CCDS6460.1. [P04090-1 ]
    PIRi A05092. A60982.
    RefSeqi NP_005050.2. NM_005059.3. [P04090-2 ]
    NP_604390.1. NM_134441.2. [P04090-1 ]
    UniGenei Hs.127032.
    Hs.368996.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    6RLX X-ray 1.50 A/C 163-185 [» ]
    B/D 26-53 [» ]
    ProteinModelPortali P04090.
    SMRi P04090. Positions 26-52.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000371040.

    PTM databases

    PhosphoSitei P04090.

    Polymorphism databases

    DMDMi 132281.

    Proteomic databases

    PaxDbi P04090.
    PRIDEi P04090.

    Protocols and materials databases

    DNASUi 6019.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000381627 ; ENSP00000371040 ; ENSG00000107014 . [P04090-1 ]
    GeneIDi 6019.
    KEGGi hsa:6019.
    UCSCi uc003ziz.2. human. [P04090-2 ]
    uc003zja.2. human. [P04090-1 ]

    Organism-specific databases

    CTDi 6019.
    GeneCardsi GC09M005305.
    HGNCi HGNC:10027. RLN2.
    MIMi 179740. gene.
    neXtProti NX_P04090.
    PharmGKBi PA34400.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG40989.
    HOGENOMi HOG000236342.
    HOVERGENi HBG003234.
    InParanoidi P04090.
    KOi K05255.
    OMAi LSEKCCQ.
    OrthoDBi EOG7QZGC5.
    PhylomeDBi P04090.
    TreeFami TF333404.

    Enzyme and pathway databases

    Reactomei REACT_19327. G alpha (s) signalling events.
    REACT_21314. Relaxin receptors.

    Miscellaneous databases

    EvolutionaryTracei P04090.
    GenomeRNAii 6019.
    NextBioi 23483.
    PROi P04090.
    SOURCEi Search...

    Gene expression databases

    Bgeei P04090.
    CleanExi HS_RLN2.
    Genevestigatori P04090.

    Family and domain databases

    Gene3Di 1.10.100.10. 2 hits.
    InterProi IPR016179. Insulin-like.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    IPR022421. Relaxin.
    [Graphical view ]
    Pfami PF00049. Insulin. 1 hit.
    [Graphical view ]
    PRINTSi PR00276. INSULINFAMLY.
    PR02004. RELAXIN.
    SMARTi SM00078. IlGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56994. SSF56994. 2 hits.
    PROSITEi PS00262. INSULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Relaxin gene expression in human ovaries and the predicted structure of a human preprorelaxin by analysis of cDNA clones."
      Hudson P., John M., Crawford R., Haralambidis J., Scanlon D., Gorman J., Tregear G., Shine J., Niall H.
      EMBO J. 3:2333-2339(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "Expression of human relaxin genes: characterization of a novel alternatively-spliced human relaxin mRNA species."
      Gunnersen J.M., Fu P., Roche P.J., Tregear G.W.
      Mol. Cell. Endocrinol. 118:85-94(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-185 (ISOFORM 2), TISSUE SPECIFICITY.
      Tissue: Prostate.
    5. "Analysis of the 5'-upstream regions of the human relaxin H1 and H2 genes and their chromosomal localization on chromosome 9p24.1 by radiation hybrid and breakpoint mapping."
      Garibay-Tupas J.L., Csiszar K., Fox M., Povey S., Bryant-Greenwood G.D.
      J. Mol. Endocrinol. 23:355-365(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
    6. "Human seminal relaxin is a product of the same gene as human luteal relaxin."
      Winslow J.W., Shih A., Bourell J.H., Weiss G., Reed B., Stults J.T., Goldsmith L.T.
      Endocrinology 130:2660-2668(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-51.
      Tissue: Semen.
    7. "Structural characterization by mass spectrometry of native and recombinant human relaxin."
      Winslow J.W., Griffin P.R., Rinderknecht E., Vandlen R.L.
      Biomed. Environ. Mass Spectrom. 19:655-664(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-53 AND 162-185 (ISOFORM 1), PYROGLUTAMATE FORMATION AT GLN-162.
    8. "Total synthesis of human relaxin and human relaxin derivatives by solid-phase peptide synthesis and site-directed chain combination."
      Buellesbach E.E., Schwabe C.
      J. Biol. Chem. 266:10754-10761(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SYNTHESIS.
    9. "X-ray structure of human relaxin at 1.5 A. Comparison to insulin and implications for receptor binding determinants."
      Eigenbrot C., Randal M., Quan C., Burnier J., O'Connell L., Rinderknecht E., Kossiakoff A.A.
      J. Mol. Biol. 221:15-21(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

    Entry informationi

    Entry nameiREL2_HUMAN
    AccessioniPrimary (citable) accession number: P04090
    Secondary accession number(s): A0AVM0
    , Q99936, Q9UCX3, Q9UQJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1986
    Last modified: October 1, 2014
    This is version 153 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3