Reviewed,
UniProtKB/Swiss-Prot P04085 (PDGFA_HUMAN)
Last modified
June 16, 2009.
Version 103.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Platelet-derived growth factor subunit A Short name=PDGF subunit A Alternative name(s): Platelet-derived growth factor A chain Platelet-derived growth factor alpha polypeptide PDGF-1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 211 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. |
| Subunit structure | Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4. Ref.10 Ref.11 |
| Subcellular location | |
| Domain | The long form contains a basic insert which acts as a cell retention signal. |
| Miscellaneous | A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. |
| Sequence similarities | Belongs to the PDGF/VEGF growth factor family. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: P04085-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: P04085-2) The sequence of this isoform differs from the canonical sequence as follows: 194-196: GRP → DVR 197-211: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 20 | 20 | |||||||||
| Propeptide | 21 – 86 | 66 | Removed in mature form | PRO_0000023356 | |||||||
| Chain | 87 – 211 | 125 | Platelet-derived growth factor subunit A | PRO_0000023357 | |||||||
Regions | |||||||||||
| Region | 158 – 162 | 5 | Receptor binding site Potential | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 134 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 96 ↔ 140 | By similarity | |||||||||
| Disulfide bond | 123 | Interchain Ref.10 | |||||||||
| Disulfide bond | 129 ↔ 177 | By similarity | |||||||||
| Disulfide bond | 132 | Interchain Ref.10 | |||||||||
| Disulfide bond | 133 ↔ 179 | By similarity | |||||||||
Natural variations | |||||||||||
| Alternative sequence | 194 – 196 | 3 | GRP → DVR in isoform Short. | VSP_004602 | |||||||
| Alternative sequence | 197 – 211 | 15 | Missing in isoform Short. | VSP_004603 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 64 – 66 | 3 | RAH → TRD in AAA60045. Ref.2 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing." Bonthron D.T., Morton C.C., Orkin S.H., Collins T. Proc. Natl. Acad. Sci. U.S.A. 85:1492-1496(1988) [PubMed: 3422746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins." Rorsman F., Bywater M., Knott T.J., Scott J., Betsholtz C. Mol. Cell. Biol. 8:571-577(1988) [PubMed: 2832727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines." Betsholtz C., Johnsson A., Heldin C.H., Westermark B., Lind P., Urdea M.S., Eddy R., Shows T.B., Philpott K., Mellor A.L., Knott T.J., Scott J. Nature 320:695-699(1986) [PubMed: 3754619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [4] | "The long 3'-untranslated regions of the PDGF-A and -B mRNAs are only distantly related." Hoppe J., Schumacher L., Eichner W., Weich H.A. FEBS Lett. 223:243-246(1987) [PubMed: 3666150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [5] | "Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. Nomura N.Nat. Methods 5:1011-1017(2008) [PubMed: 19054851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). |
| [6] | "Nucleotide sequence of the 5' region of the human platelet-derived growth factor A-chain gene." Takimoto Y., Li W.Y., Wang Z.Y., Tong B.D., Deuel T.F. Hiroshima J. Med. Sci. 42:47-52(1993) [PubMed: 8486521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53. |
| [7] | "Platelet-derived growth factor A chain: confirmation of localization of PDGFA to chromosome 7p22 and description of an unusual minisatellite." Bonthron D., Collins T., Grzeschik K.H., van Roy N., Speleman F. Genomics 13:257-263(1992) [PubMed: 1612586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 152-211. |
| [8] | "cDNA clones reveal differences between human glial and endothelial cell platelet-derived growth factor A-chains." Tong B.D., Auer D.E., Jaye M., Kaplow J.M., Ricca G., McConathy E., Drohan W., Deuel T.F. Nature 328:619-621(1987) [PubMed: 3614363] [Abstract] Cited for: ALTERNATIVE SPLICING. |
| [9] | "Alternative RNA splicing affects function of encoded platelet-derived growth factor A chain." Collins T., Bonthron D.T., Orkin S.H. Nature 328:621-624(1987) [PubMed: 3614364] [Abstract] Cited for: ALTERNATIVE SPLICING. |
| [10] | "Assignment of interchain disulfide bonds in platelet-derived growth factor (PDGF) and evidence for agonist activity of monomeric PDGF." Andersson M., Oestman A., Baeckstroem G., Hellman U., George-Nascimento C., Westermark B., Heldin C.-H. J. Biol. Chem. 267:11260-11266(1992) [PubMed: 1317862] [Abstract] Cited for: INTERCHAIN DISULFIDE BONDS. |
| [11] | "High-affinity binding of basic fibroblast growth factor and platelet-derived growth factor-AA to the core protein of the NG2 proteoglycan." Goretzki L., Burg M.A., Grako K.A., Stallcup W.B. J. Biol. Chem. 274:16831-16837(1999) [PubMed: 10358027] [Abstract] Cited for: INTERACTION WITH CSPG4. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X03795 mRNA. Translation: CAA27421.1. X06374 mRNA. Translation: CAA29677.1. M20494 M20493 Genomic DNA. Translation: AAA60045.1. M19988 M19987 Genomic DNA. Translation: AAA60046.1. M19989 M19987 Genomic DNA. Translation: AAA60047.1. A09204 Unassigned RNA. Translation: CAA00830.1. AB451309 mRNA. Translation: BAG70123.1. AB451439 mRNA. Translation: BAG70253.1. S62078 Genomic DNA. Translation: AAB26566.1. | |
| IPI | IPI00021833. IPI00220454. |
| PIR | PFHUG1. A28964. B28964. |
| RefSeq | NP_002598.4. NP_148983.1. |
| UniGene | Hs.535898 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1PDG based on UniProtKB P01127. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:5735N. |
Proteomic databases | |
| PRIDE | P04085. |
Genome annotation databases | |
| Ensembl | ENSG00000197461. Homo sapiens. [Contig view] |
| GeneID | 5154. |
| KEGG | hsa:5154. |
Organism-specific databases | |
| GeneCards | GC07M000503. |
| H-InvDB | HIX0042124. |
| HGNC | HGNC:8799. PDGFA. |
| HPA | CAB005579. |
| MIM | 173430. gene. |
| PharmGKB | PA33144. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P04085. |
| HOVERGEN | P04085. |
| OMA | P04085. DYREEET. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | ceramidepathway. Ceramide signaling pathway. pdgfrapathway. PDGFR-alpha signaling pathway. |
| Reactome | REACT_604. Hemostasis. |
Gene expression databases | |
| ArrayExpress | P04085. |
| Bgee | P04085. |
| CleanEx | HS_PDGFA. |
| GermOnline | ENSG00000197461. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000072. PD_growth_factor. IPR006782. PDGF_N. [Graphical view] |
| Pfam | PF00341. PDGF. 1 hit. PF04692. PDGF_N. 1 hit. [Graphical view] |
| ProDom | PD001629. PD_growth_factor. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00141. PDGF. 1 hit. [Graphical view] |
| PROSITE | PS00249. PDGF_1. 1 hit. PS50278. PDGF_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 19930. |
| SOURCE | Search... |
Entry information
| Entry name | PDGFA_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P04085 Secondary accession number(s): B5BU73 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 7 Human chromosome 7: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


