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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of GMP from XMP.

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Enzyme regulationi

Inhibited by 2-fluoroinosine 5'-monophosphate (F-IMP) and by N(2)-hydroxyguanosine 5'-monophosphate (N(2)-OH-GMP).1 Publication

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Nucleophile1
Active sitei1811
Active sitei1831

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi235 – 241ATP7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • GMP synthase (glutamine-hydrolyzing) activity Source: EcoCyc
  • GMP synthase activity Source: EcoCyc
  • pyrophosphatase activity Source: InterPro

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-HAMAP
  • GMP biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:GMP-SYN-MONOMER.
ECOL316407:JW2491-MONOMER.
MetaCyc:GMP-SYN-MONOMER.
BRENDAi6.3.5.2. 2026.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.957.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Short name:
GMPS
Glutamine amidotransferase
Gene namesi
Name:guaA
Ordered Locus Names:b2507, JW2491
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10420. guaA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001401231 – 525GMP synthase [glutamine-hydrolyzing]Add BLAST525

Proteomic databases

EPDiP04079.
PaxDbiP04079.
PRIDEiP04079.

Expressioni

Inductioni

Activated by cAMP receptor protein (CRP). Repressed by DnaA.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-909122,EBI-909122

Protein-protein interaction databases

BioGridi4259645. 32 interactors.
DIPiDIP-9852N.
IntActiP04079. 8 interactors.
STRINGi511145.b2507.

Structurei

Secondary structure

1525
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 14Combined sources8
Helixi20 – 29Combined sources10
Beta strandi33 – 39Combined sources7
Helixi43 – 49Combined sources7
Beta strandi52 – 56Combined sources5
Helixi73 – 76Combined sources4
Beta strandi78 – 80Combined sources3
Beta strandi82 – 85Combined sources4
Helixi87 – 95Combined sources9
Beta strandi99 – 101Combined sources3
Beta strandi107 – 115Combined sources9
Turni120 – 124Combined sources5
Beta strandi127 – 129Combined sources3
Beta strandi135 – 143Combined sources9
Beta strandi145 – 149Combined sources5
Beta strandi155 – 159Combined sources5
Beta strandi166 – 170Combined sources5
Turni171 – 174Combined sources4
Beta strandi175 – 180Combined sources6
Helixi190 – 199Combined sources10
Helixi210 – 225Combined sources16
Beta strandi229 – 233Combined sources5
Helixi238 – 251Combined sources14
Helixi252 – 254Combined sources3
Beta strandi255 – 261Combined sources7
Helixi269 – 277Combined sources9
Turni278 – 281Combined sources4
Beta strandi285 – 289Combined sources5
Helixi291 – 298Combined sources8
Helixi304 – 325Combined sources22
Beta strandi326 – 333Combined sources8
Helixi338 – 343Combined sources6
Beta strandi371 – 373Combined sources3
Turni375 – 378Combined sources4
Helixi381 – 390Combined sources10
Helixi395 – 398Combined sources4
Helixi407 – 410Combined sources4
Helixi418 – 437Combined sources20
Helixi441 – 443Combined sources3
Beta strandi444 – 457Combined sources14
Turni460 – 462Combined sources3
Beta strandi465 – 479Combined sources15
Beta strandi482 – 485Combined sources4
Helixi490 – 503Combined sources14
Beta strandi507 – 513Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GPMX-ray2.20A/B/C/D1-525[»]
ProteinModelPortaliP04079.
SMRiP04079.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04079.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 207Glutamine amidotransferase type-1Add BLAST199
Domaini208 – 400GMPS ATP-PPaseAdd BLAST193

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
InParanoidiP04079.
KOiK01951.
OMAiMILVLDF.
PhylomeDBiP04079.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTENIHKHRI LILDFGSQYT QLVARRVREL GVYCELWAWD VTEAQIRDFN
60 70 80 90 100
PSGIILSGGP ESTTEENSPR APQYVFEAGV PVFGVCYGMQ TMAMQLGGHV
110 120 130 140 150
EASNEREFGY AQVEVVNDSA LVRGIEDALT ADGKPLLDVW MSHGDKVTAI
160 170 180 190 200
PSDFITVAST ESCPFAIMAN EEKRFYGVQF HPEVTHTRQG MRMLERFVRD
210 220 230 240 250
ICQCEALWTP AKIIDDAVAR IREQVGDDKV ILGLSGGVDS SVTAMLLHRA
260 270 280 290 300
IGKNLTCVFV DNGLLRLNEA EQVLDMFGDH FGLNIVHVPA EDRFLSALAG
310 320 330 340 350
ENDPEAKRKI IGRVFVEVFD EEALKLEDVK WLAQGTIYPD VIESAASATG
360 370 380 390 400
KAHVIKSHHN VGGLPKEMKM GLVEPLKELF KDEVRKIGLE LGLPYDMLYR
410 420 430 440 450
HPFPGPGLGV RVLGEVKKEY CDLLRRADAI FIEELRKADL YDKVSQAFTV
460 470 480 490 500
FLPVRSVGVM GDGRKYDWVV SLRAVETIDF MTAHWAHLPY DFLGRVSNRI
510 520
INEVNGISRV VYDISGKPPA TIEWE
Length:525
Mass (Da):58,679
Last modified:January 1, 1988 - v1
Checksum:iD934786DFF3D694B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3E → N AA sequence (PubMed:3894345).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10101 Genomic DNA. Translation: AAB18619.1.
U00096 Genomic DNA. Translation: AAC75560.1.
AP009048 Genomic DNA. Translation: BAA16394.1.
PIRiA24640. SYECGU.
RefSeqiNP_417002.1. NC_000913.3.
WP_000138270.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75560; AAC75560; b2507.
BAA16394; BAA16394; BAA16394.
GeneIDi947334.
KEGGiecj:JW2491.
eco:b2507.
PATRICi32120405. VBIEscCol129921_2605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10101 Genomic DNA. Translation: AAB18619.1.
U00096 Genomic DNA. Translation: AAC75560.1.
AP009048 Genomic DNA. Translation: BAA16394.1.
PIRiA24640. SYECGU.
RefSeqiNP_417002.1. NC_000913.3.
WP_000138270.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GPMX-ray2.20A/B/C/D1-525[»]
ProteinModelPortaliP04079.
SMRiP04079.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259645. 32 interactors.
DIPiDIP-9852N.
IntActiP04079. 8 interactors.
STRINGi511145.b2507.

Protein family/group databases

MEROPSiC26.957.

Proteomic databases

EPDiP04079.
PaxDbiP04079.
PRIDEiP04079.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75560; AAC75560; b2507.
BAA16394; BAA16394; BAA16394.
GeneIDi947334.
KEGGiecj:JW2491.
eco:b2507.
PATRICi32120405. VBIEscCol129921_2605.

Organism-specific databases

EchoBASEiEB0415.
EcoGeneiEG10420. guaA.

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
InParanoidiP04079.
KOiK01951.
OMAiMILVLDF.
PhylomeDBiP04079.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BioCyciEcoCyc:GMP-SYN-MONOMER.
ECOL316407:JW2491-MONOMER.
MetaCyc:GMP-SYN-MONOMER.
BRENDAi6.3.5.2. 2026.

Miscellaneous databases

EvolutionaryTraceiP04079.
PROiP04079.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAA_ECOLI
AccessioniPrimary (citable) accession number: P04079
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.