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Protein

Glutamine synthetase cytosolic isozyme

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001531831 – 356Glutamine synthetase cytosolic isozymeAdd BLAST356

Proteomic databases

PRIDEiP04078.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP04078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P04078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLSDLINL DLSETTEKII AEYIWIGGSG LDLRSKARTL PGPVTDPSQL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV IIYPQAIFKD PFRRGNNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRHAA AKIFSHPDVV AEVPWYGIEQ EYTLLQKDIN WPLGWPVGGF
160 170 180 190 200
PGPQGPYYCG AGADKAFGRD IVDSHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA GDEIWVARYI LERITEVAGV VLSFDPKPIK GDWNGAGAHT
260 270 280 290 300
NYSTKSMRED GGYEVILKAI EKLGKKHKEH IAAYGEGNER RLTGRHETAD
310 320 330 340 350
INTFLWGVAN RGASIRVGRD TEKAGKGYFE DRRPSSNMDP YVVTSMIADT

TILWKP
Length:356
Mass (Da):39,107
Last modified:November 1, 1986 - v1
Checksum:iB4450F5489B27BD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03282 mRNA. Translation: AAA32654.1.
X03931 Genomic DNA. Translation: CAA27570.1.
PIRiA26025. AJAAQ.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03282 mRNA. Translation: AAA32654.1.
X03931 Genomic DNA. Translation: CAA27570.1.
PIRiA26025. AJAAQ.

3D structure databases

ProteinModelPortaliP04078.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP04078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA1_MEDSA
AccessioniPrimary (citable) accession number: P04078
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: October 5, 2016
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.