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P04078

- GLNA1_MEDSA

UniProt

P04078 - GLNA1_MEDSA

Protein

Glutamine synthetase cytosolic isozyme

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Nov 1986)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase
    OrganismiMedicago sativa (Alfalfa)
    Taxonomic identifieri3879 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Glutamine synthetase cytosolic isozymePRO_0000153183Add
    BLAST

    Proteomic databases

    ProMEXiP04078.

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP04078.
    SMRiP04078. Positions 4-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04078-1 [UniParc]FASTAAdd to Basket

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    MSLLSDLINL DLSETTEKII AEYIWIGGSG LDLRSKARTL PGPVTDPSQL    50
    PKWNYDGSST GQAPGEDSEV IIYPQAIFKD PFRRGNNILV MCDAYTPAGE 100
    PIPTNKRHAA AKIFSHPDVV AEVPWYGIEQ EYTLLQKDIN WPLGWPVGGF 150
    PGPQGPYYCG AGADKAFGRD IVDSHYKACL YAGINISGIN GEVMPGQWEF 200
    QVGPSVGISA GDEIWVARYI LERITEVAGV VLSFDPKPIK GDWNGAGAHT 250
    NYSTKSMRED GGYEVILKAI EKLGKKHKEH IAAYGEGNER RLTGRHETAD 300
    INTFLWGVAN RGASIRVGRD TEKAGKGYFE DRRPSSNMDP YVVTSMIADT 350
    TILWKP 356
    Length:356
    Mass (Da):39,107
    Last modified:November 1, 1986 - v1
    Checksum:iB4450F5489B27BD7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03282 mRNA. Translation: AAA32654.1.
    X03931 Genomic DNA. Translation: CAA27570.1.
    PIRiA26025. AJAAQ.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K03282 mRNA. Translation: AAA32654.1 .
    X03931 Genomic DNA. Translation: CAA27570.1 .
    PIRi A26025. AJAAQ.

    3D structure databases

    ProteinModelPortali P04078.
    SMRi P04078. Positions 4-355.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    ProMEXi P04078.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of an alfalfa glutamine synthetase gene."
      Tischer E., Dassarma S., Goodman H.M.
      Mol. Gen. Genet. 203:221-229(1986)
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Herbicide-resistant alfalfa cells: an example of gene amplification in plants."
      Donn G., Tischer E., Smith J.A., Goodman H.M.
      J. Mol. Appl. Genet. 2:621-635(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 266-356.

    Entry informationi

    Entry nameiGLNA1_MEDSA
    AccessioniPrimary (citable) accession number: P04078
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1986
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3