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P04078 (GLNA1_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase cytosolic isozyme

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutamine synthetase cytosolic isozyme
PRO_0000153183

Sequences

Sequence LengthMass (Da)Tools
P04078 [UniParc].

Last modified November 1, 1986. Version 1.
Checksum: B4450F5489B27BD7

FASTA35639,107
        10         20         30         40         50         60 
MSLLSDLINL DLSETTEKII AEYIWIGGSG LDLRSKARTL PGPVTDPSQL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGEDSEV IIYPQAIFKD PFRRGNNILV MCDAYTPAGE PIPTNKRHAA AKIFSHPDVV 

       130        140        150        160        170        180 
AEVPWYGIEQ EYTLLQKDIN WPLGWPVGGF PGPQGPYYCG AGADKAFGRD IVDSHYKACL 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPSVGISA GDEIWVARYI LERITEVAGV VLSFDPKPIK 

       250        260        270        280        290        300 
GDWNGAGAHT NYSTKSMRED GGYEVILKAI EKLGKKHKEH IAAYGEGNER RLTGRHETAD 

       310        320        330        340        350 
INTFLWGVAN RGASIRVGRD TEKAGKGYFE DRRPSSNMDP YVVTSMIADT TILWKP 

« Hide

References

[1]"Nucleotide sequence of an alfalfa glutamine synthetase gene."
Tischer E., Dassarma S., Goodman H.M.
Mol. Gen. Genet. 203:221-229(1986)
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Herbicide-resistant alfalfa cells: an example of gene amplification in plants."
Donn G., Tischer E., Smith J.A., Goodman H.M.
J. Mol. Appl. Genet. 2:621-635(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 266-356.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K03282 mRNA. Translation: AAA32654.1.
X03931 Genomic DNA. Translation: CAA27570.1.
PIRAJAAQ. A26025.

3D structure databases

ProteinModelPortalP04078.
SMRP04078. Positions 4-355.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

ProMEXP04078.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA1_MEDSA
AccessionPrimary (citable) accession number: P04078
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families