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Protein

Argininosuccinate lyase

Gene

ARG4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-(N(omega)-L-arginino)succinate = fumarate + L-arginine.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (ARG3)
  2. Argininosuccinate synthase (ARG1)
  3. Argininosuccinate lyase (ARG4)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • argininosuccinate lyase activity Source: SGD

GO - Biological processi

  • arginine biosynthetic process via ornithine Source: SGD
  • protein tetramerization Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YHR018C-MONOMER.
ReactomeiR-SCE-70635. Urea cycle.
UniPathwayiUPA00068; UER00114.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate lyase (EC:4.3.2.1)
Short name:
ASAL
Alternative name(s):
Arginosuccinase
Gene namesi
Name:ARG4
Ordered Locus Names:YHR018C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR018C.
SGDiS000001060. ARG4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001377271 – 463Argininosuccinate lyaseAdd BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04076.
PRIDEiP04076.
TopDownProteomicsiP04076.

PTM databases

iPTMnetiP04076.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi36447. 15 interactors.
DIPiDIP-4306N.
IntActiP04076. 1 interactor.
MINTiMINT-560098.

Structurei

3D structure databases

ProteinModelPortaliP04076.
SMRiP04076.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000014045.
HOGENOMiHOG000242744.
InParanoidiP04076.
KOiK01755.
OMAiAHHLMAY.
OrthoDBiEOG092C22ZV.

Family and domain databases

CDDicd01359. Argininosuccinate_lyase. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00006. Arg_succ_lyase. 1 hit.
InterProiIPR029419. Arg_succ_lyase_C.
IPR009049. Argininosuccinate_lyase.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PTHR11444:SF3. PTHR11444:SF3. 1 hit.
PfamiPF14698. ASL_C2. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00838. argH. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGTQKLWG GRFTGETDPL MHLYNASLPY DYKMYKADLE GTKVYTAGLQ
60 70 80 90 100
KLGLLTETEL AKIHEGLAEI KKEWDADKFV RHPNDEDIHT ANERRLGELI
110 120 130 140 150
GRDIAGKVHT GRSRNDQVVT DLRIYCRDIV NDTLFPALKG LVEVLIKRAE
160 170 180 190 200
GEIDVLMPGY THLQRAQPIR WSHWLSSYAT YFTEDYKRLG QILHRLNQSP
210 220 230 240 250
LGAGALAGHP YGIDREFLAE GLGFNSVIGN SLVAVSDRDF IVELMFWGTL
260 270 280 290 300
FMNHISRFAE DLIIYCTAEF GFIQLSDAYS TGSSLMPQKK NADSLELLRG
310 320 330 340 350
KSGRVFGDLT GFLMSLKGIP STYDKDMQED KEPLFDCLTT VEHSMLIATG
360 370 380 390 400
VISTLTVNKE KMEAALTMDM LATDLADYLV RKGVPFRETH HISGECVATA
410 420 430 440 450
ERLGLSGIDK LTLEQYQKID SRFGQDLFET FNFEQSVERR DATGGTAKSA
460
VLKQLDNLKS QLN
Length:463
Mass (Da):51,989
Last modified:February 1, 1995 - v2
Checksum:i2E5C01E53B3E163B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103D → E in AAA34434 (PubMed:6386606).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36586 Genomic DNA. Translation: AAA34435.1.
K01813 Genomic DNA. Translation: AAA34434.1.
AY693155 Genomic DNA. Translation: AAT93174.1.
U10400 Genomic DNA. Translation: AAB68946.1.
BK006934 Genomic DNA. Translation: DAA06707.1.
PIRiS46792. WZBYRS.
RefSeqiNP_011882.1. NM_001179148.1.

Genome annotation databases

EnsemblFungiiYHR018C; YHR018C; YHR018C.
GeneIDi856411.
KEGGisce:YHR018C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36586 Genomic DNA. Translation: AAA34435.1.
K01813 Genomic DNA. Translation: AAA34434.1.
AY693155 Genomic DNA. Translation: AAT93174.1.
U10400 Genomic DNA. Translation: AAB68946.1.
BK006934 Genomic DNA. Translation: DAA06707.1.
PIRiS46792. WZBYRS.
RefSeqiNP_011882.1. NM_001179148.1.

3D structure databases

ProteinModelPortaliP04076.
SMRiP04076.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36447. 15 interactors.
DIPiDIP-4306N.
IntActiP04076. 1 interactor.
MINTiMINT-560098.

PTM databases

iPTMnetiP04076.

Proteomic databases

MaxQBiP04076.
PRIDEiP04076.
TopDownProteomicsiP04076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR018C; YHR018C; YHR018C.
GeneIDi856411.
KEGGisce:YHR018C.

Organism-specific databases

EuPathDBiFungiDB:YHR018C.
SGDiS000001060. ARG4.

Phylogenomic databases

GeneTreeiENSGT00390000014045.
HOGENOMiHOG000242744.
InParanoidiP04076.
KOiK01755.
OMAiAHHLMAY.
OrthoDBiEOG092C22ZV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00114.
BioCyciYEAST:YHR018C-MONOMER.
ReactomeiR-SCE-70635. Urea cycle.

Miscellaneous databases

PROiP04076.

Family and domain databases

CDDicd01359. Argininosuccinate_lyase. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00006. Arg_succ_lyase. 1 hit.
InterProiIPR029419. Arg_succ_lyase_C.
IPR009049. Argininosuccinate_lyase.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PTHR11444:SF3. PTHR11444:SF3. 1 hit.
PfamiPF14698. ASL_C2. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00838. argH. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARLY_YEAST
AccessioniPrimary (citable) accession number: P04076
Secondary accession number(s): D3DKW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.