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Protein

Argininosuccinate lyase

Gene

ARG4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 10200 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2-(N(omega)-L-arginino)succinate = fumarate + L-arginine.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (ARG3)
  2. Argininosuccinate synthase (ARG1)
  3. Argininosuccinate lyase (ARG4)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • argininosuccinate lyase activity Source: SGD

GO - Biological processi

  • arginine biosynthetic process via ornithine Source: SGD

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YHR018C-MONOMER
ReactomeiR-SCE-70635 Urea cycle
UniPathwayiUPA00068; UER00114

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate lyase (EC:4.3.2.1)
Short name:
ASAL
Alternative name(s):
Arginosuccinase
Gene namesi
Name:ARG4
Ordered Locus Names:YHR018C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR018C
SGDiS000001060 ARG4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001377271 – 463Argininosuccinate lyaseAdd BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04076
PaxDbiP04076
PRIDEiP04076
TopDownProteomicsiP04076

PTM databases

iPTMnetiP04076

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi36447, 17 interactors
DIPiDIP-4306N
IntActiP04076, 3 interactors
STRINGi4932.YHR018C

Structurei

3D structure databases

ProteinModelPortaliP04076
SMRiP04076
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000014045
HOGENOMiHOG000242744
InParanoidiP04076
KOiK01755
OMAiSIMPHKK
OrthoDBiEOG092C22ZV

Family and domain databases

CDDicd01359 Argininosuccinate_lyase, 1 hit
Gene3Di1.10.275.10, 2 hits
HAMAPiMF_00006 Arg_succ_lyase, 1 hit
InterProiView protein in InterPro
IPR029419 Arg_succ_lyase_C
IPR009049 Argininosuccinate_lyase
IPR024083 Fumarase/histidase_N
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR43814 PTHR43814, 1 hit
PfamiView protein in Pfam
PF14698 ASL_C2, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00838 argH, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

P04076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGTQKLWG GRFTGETDPL MHLYNASLPY DYKMYKADLE GTKVYTAGLQ
60 70 80 90 100
KLGLLTETEL AKIHEGLAEI KKEWDADKFV RHPNDEDIHT ANERRLGELI
110 120 130 140 150
GRDIAGKVHT GRSRNDQVVT DLRIYCRDIV NDTLFPALKG LVEVLIKRAE
160 170 180 190 200
GEIDVLMPGY THLQRAQPIR WSHWLSSYAT YFTEDYKRLG QILHRLNQSP
210 220 230 240 250
LGAGALAGHP YGIDREFLAE GLGFNSVIGN SLVAVSDRDF IVELMFWGTL
260 270 280 290 300
FMNHISRFAE DLIIYCTAEF GFIQLSDAYS TGSSLMPQKK NADSLELLRG
310 320 330 340 350
KSGRVFGDLT GFLMSLKGIP STYDKDMQED KEPLFDCLTT VEHSMLIATG
360 370 380 390 400
VISTLTVNKE KMEAALTMDM LATDLADYLV RKGVPFRETH HISGECVATA
410 420 430 440 450
ERLGLSGIDK LTLEQYQKID SRFGQDLFET FNFEQSVERR DATGGTAKSA
460
VLKQLDNLKS QLN
Length:463
Mass (Da):51,989
Last modified:February 1, 1995 - v2
Checksum:i2E5C01E53B3E163B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103D → E in AAA34434 (PubMed:6386606).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36586 Genomic DNA Translation: AAA34435.1
K01813 Genomic DNA Translation: AAA34434.1
AY693155 Genomic DNA Translation: AAT93174.1
U10400 Genomic DNA Translation: AAB68946.1
BK006934 Genomic DNA Translation: DAA06707.1
PIRiS46792 WZBYRS
RefSeqiNP_011882.1, NM_001179148.1

Genome annotation databases

EnsemblFungiiYHR018C; YHR018C; YHR018C
GeneIDi856411
KEGGisce:YHR018C

Similar proteinsi

Entry informationi

Entry nameiARLY_YEAST
AccessioniPrimary (citable) accession number: P04076
Secondary accession number(s): D3DKW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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