P04076 (ARLY_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate lyase
Short name=ASAL
EC=4.3.2.1

Alternative name(s):
Arginosuccinase

Gene names

Name:	ARG4
Ordered Locus Names:	YHR018C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Subunit structure	Homotetramer.
Miscellaneous	Present with 10200 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from direct assay. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD
Molecular function	argininosuccinate lyase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Argininosuccinate lyase		PRO_0000137727
Amino acid modifications
	Modified residue	27	1	Phosphoserine Ref.8
	Modified residue	443	1	Phosphothreonine Ref.7
Experimental info
	Sequence conflict	103	1	D → E in AAA34434. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P04076 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 2E5C01E53B3E163B	FASTA	463	51,989
	10 20 30 40 50 60 MSDGTQKLWG GRFTGETDPL MHLYNASLPY DYKMYKADLE GTKVYTAGLQ KLGLLTETEL 70 80 90 100 110 120 AKIHEGLAEI KKEWDADKFV RHPNDEDIHT ANERRLGELI GRDIAGKVHT GRSRNDQVVT 130 140 150 160 170 180 DLRIYCRDIV NDTLFPALKG LVEVLIKRAE GEIDVLMPGY THLQRAQPIR WSHWLSSYAT 190 200 210 220 230 240 YFTEDYKRLG QILHRLNQSP LGAGALAGHP YGIDREFLAE GLGFNSVIGN SLVAVSDRDF 250 260 270 280 290 300 IVELMFWGTL FMNHISRFAE DLIIYCTAEF GFIQLSDAYS TGSSLMPQKK NADSLELLRG 310 320 330 340 350 360 KSGRVFGDLT GFLMSLKGIP STYDKDMQED KEPLFDCLTT VEHSMLIATG VISTLTVNKE 370 380 390 400 410 420 KMEAALTMDM LATDLADYLV RKGVPFRETH HISGECVATA ERLGLSGIDK LTLEQYQKID 430 440 450 460 SRFGQDLFET FNFEQSVERR DATGGTAKSA VLKQLDNLKS QLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the yeast ARG4 gene." Beacham I.R., Schweitzer B.W., Warrick H.M., Carbon J. Gene 29:271-279(1984) [PubMed: 6386606] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Mismatch-specific post-meiotic segregation frequency in yeast suggests a heteroduplex recombination intermediate." White J.H., Lusnak K., Fogel S. Nature 315:350-352(1985) [PubMed: 3889658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-443, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36586 Genomic DNA. Translation: AAA34435.1. K01813 Genomic DNA. Translation: AAA34434.1. AY693155 Genomic DNA. Translation: AAT93174.1. U10400 Genomic DNA. Translation: AAB68946.1. BK006934 Genomic DNA. Translation: DAA06707.1.
PIR	WZBYRS. S46792.
RefSeq	NP_011882.1. NM_001179148.1.
3D structure databases
ProteinModelPortal	P04076.
SMR	P04076. Positions 6-463.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4306N.
IntAct	P04076. 3 interactions.
MINT	MINT-560098.
STRING	P04076.
Proteomic databases
PeptideAtlas	P04076.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR018C; YHR018C; YHR018C.
GeneID	856411.
KEGG	sce:YHR018C.
NMPDR	fig|4932.3.peg.3027.
Organism-specific databases
CYGD	YHR018c.
SGD	S000001060. ARG4.
Phylogenomic databases
eggNOG	fuNOG04635.
GeneTree	EFGT00050000004720.
HOGENOM	HBG539632.
OMA	MAEDLIF.
OrthoDB	EOG4VMJQ2.
Gene expression databases
ArrayExpress	P04076.
Genevestigator	P04076.
GermOnline	YHR018C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR022761. Lyase1_N. [Graphical view]
KO	K01755.
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00838. ArgH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981962.

Entry information

Entry name

ARLY_YEAST

Accession

Primary (citable) accession number: P04076
Secondary accession number(s): D3DKW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program