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P04074

- AT1A1_SHEEP

UniProt

P04074 - AT1A1_SHEEP

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Protein
Sodium/potassium-transporting ATPase subunit alpha-1
Gene
ATP1A1
Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Enzyme regulationi

Specifically inhibited by cardiac glycosides such as digoxin or ouabain.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei374 – 37414-aspartylphosphate intermediate By similarity
Binding sitei485 – 4851ATP By similarity
Metal bindingi715 – 7151Magnesium By similarity
Metal bindingi719 – 7191Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sodium:potassium-exchanging ATPase activity Source: UniProtKB

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. negative regulation of glucocorticoid biosynthetic process Source: Ensembl
  3. negative regulation of heart contraction Source: Ensembl
  4. positive regulation of heart contraction Source: Ensembl
  5. positive regulation of striated muscle contraction Source: Ensembl
  6. regulation of blood pressure Source: Ensembl
  7. regulation of sodium ion transport Source: UniProtKB
  8. regulation of the force of heart contraction Source: Ensembl
  9. response to drug Source: Ensembl
  10. sodium ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-1 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-1 subunit
Alternative name(s):
Sodium pump subunit alpha-1
Gene namesi
Name:ATP1A1
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8580Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei86 – 10621Helical; Reviewed prediction
Add
BLAST
Topological domaini107 – 12923Extracellular Reviewed prediction
Add
BLAST
Transmembranei130 – 15021Helical; Reviewed prediction
Add
BLAST
Topological domaini151 – 286136Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei287 – 30620Helical; Reviewed prediction
Add
BLAST
Topological domaini307 – 31812Extracellular Reviewed prediction
Add
BLAST
Transmembranei319 – 33618Helical; Reviewed prediction
Add
BLAST
Topological domaini337 – 770434Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei771 – 79020Helical; Reviewed prediction
Add
BLAST
Topological domaini791 – 80010Extracellular Reviewed prediction
Transmembranei801 – 82121Helical; Reviewed prediction
Add
BLAST
Topological domaini822 – 84120Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei842 – 86423Helical; Reviewed prediction
Add
BLAST
Topological domaini865 – 91652Extracellular Reviewed prediction
Add
BLAST
Transmembranei917 – 93620Helical; Reviewed prediction
Add
BLAST
Topological domaini937 – 94913Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei950 – 96819Helical; Reviewed prediction
Add
BLAST
Topological domaini969 – 98315Extracellular Reviewed prediction
Add
BLAST
Transmembranei984 – 100421Helical; Reviewed prediction
Add
BLAST
Topological domaini1005 – 102117Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. T-tubule Source: Ensembl
  2. basolateral plasma membrane Source: Ensembl
  3. integral component of membrane Source: UniProtKB
  4. melanosome Source: UniProtKB-SubCell
  5. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55 By similarity
PRO_0000002491
Chaini6 – 10211016Sodium/potassium-transporting ATPase subunit alpha-1
PRO_0000002492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91N6-acetyllysine By similarity
Modified residuei10 – 101Phosphotyrosine By similarity
Modified residuei16 – 161Phosphoserine; by PKC By similarity
Modified residuei21 – 211N6-acetyllysine By similarity
Modified residuei258 – 2581Phosphotyrosine By similarity
Modified residuei540 – 5401Phosphotyrosine By similarity
Modified residuei941 – 9411Phosphoserine; by PKA By similarity

Post-translational modificationi

Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-941 by PKA modulates the response of ATP1A1 to PKC By similarity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Interactioni

Subunit structurei

Interacts with SIK1 By similarity. Composed of three subunits: alpha (catalytic), beta and gamma.

Structurei

3D structure databases

ProteinModelPortaliP04074.
SMRiP04074. Positions 24-1021.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 823Phosphoinositide-3 kinase binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00560000076866.
HOVERGENiHBG004298.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04074-1 [UniParc]FASTAAdd to Basket

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MGKGVGRDKY EPAAVSEHGD KKKAKKERDM DELKKEVSMD DHKLSLDELH     50
RKYGTDLNRG LTTARAAEIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSM 100
LLWIGAVLCF LAYGIQAATE EEPQNDNLYL GVVLSAVVII TGCFSYYQEA 150
KSSKIMESFK NMVPQQALVI RNGEKMSINA EEVVVGDLVE VKGGDRIPAD 200
LRIISANGCK VDNSSLTGES EPQTRSPDFT NENPLETRNI AFFSTNCVEG 250
TARGIVVYTG DRTVMGRIAT LASGLEGGQT PIAAEIEHFI HIITGVAVFL 300
GVSFFILSLI LEYTWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA 350
RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT 400
TENQSGVSFD KTSATWLALS RIAGLCNRAV FQANQDNLPI LKRAVAGDAS 450
ESALLKCIEV CCGSVKEMRE RYAKIVEIPF NSTNKYQLSI HKNANAGEPR 500
HLLVMKGAPE RILDRCSSIL IHGKEQPLDE ELKDAFQNAY LELGGLGERV 550
LGFCHLMLPD EQFPEGFQFD TDDVNFPVDN LCFVGLISMI DPPRAAVPDA 600
VGKCRSAGIK VIMVTGDHPI TAKAIAKGVG IISEGNETVE DIAARLNIPV 650
SQVNPRDARA CVVHGSDLKD MTPEQLDDIL KYHTEIVFAR TSPQQKLIIV 700
EGCQRQGAIV AVTGDGVNDS PALKKADIGV AMGIAGSDVS KQAADMILLD 750
DNFASIVTGV EEGRLIFDNL KKSIAYTLTS NIPEITPFLI FIIANIPLPL 800
GTVTILCIDL GTDMVPAISL AYEQAESDIM KRQPRNPQTD KLVNERLISM 850
AYGQIGMIQA LGGFFTYFVI MAENGFLPNH LLGIRVTWDD RWINDVEDSY 900
GQQWTYEQRK IVEFTCHTAF FVSIVVVQWA DLVICKTRRN SVFQQGMKNK 950
ILIFGLFEET ALAAFLSYCP GMGVALRMYP LKPTWWFCAF PYSLLIFVYD 1000
EVRKLIIRRR PGGWVEKETY Y 1021
Length:1,021
Mass (Da):112,658
Last modified:November 1, 1986 - v1
Checksum:i0D493D95BD9D4312
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02813 mRNA. Translation: CAA26581.1.
X02813 mRNA. Translation: CAA26582.1.
PIRiA01074. PWSHNA.
RefSeqiNP_001009360.1. NM_001009360.1.
UniGeneiOar.424.

Genome annotation databases

EnsembliENSOART00000022009; ENSOARP00000021706; ENSOARG00000020213.
GeneIDi443381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02813 mRNA. Translation: CAA26581.1 .
X02813 mRNA. Translation: CAA26582.1 .
PIRi A01074. PWSHNA.
RefSeqi NP_001009360.1. NM_001009360.1.
UniGenei Oar.424.

3D structure databases

ProteinModelPortali P04074.
SMRi P04074. Positions 24-1021.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSOART00000022009 ; ENSOARP00000021706 ; ENSOARG00000020213 .
GeneIDi 443381.

Organism-specific databases

CTDi 476.

Phylogenomic databases

GeneTreei ENSGT00560000076866.
HOVERGENi HBG004298.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA."
    Shull G.E., Schwartz A., Lingrel J.B.
    Nature 316:691-695(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Lysine 480 is an essential residue in the putative ATP site of lamb kidney (Na,K)-ATPase. Identification of the pyridoxal 5'-diphospho-5'-adenosine and pyridoxal phosphate reactive residue."
    Hinz H.R., Kirley T.L.
    J. Biol. Chem. 265:10260-10265(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 475-492.

Entry informationi

Entry nameiAT1A1_SHEEP
AccessioniPrimary (citable) accession number: P04074
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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