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P04074

- AT1A1_SHEEP

UniProt

P04074 - AT1A1_SHEEP

Protein

Sodium/potassium-transporting ATPase subunit alpha-1

Gene

ATP1A1

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1986)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Enzyme regulationi

    Specifically inhibited by cardiac glycosides such as digoxin or ouabain.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei374 – 37414-aspartylphosphate intermediateBy similarity
    Binding sitei485 – 4851ATPBy similarity
    Metal bindingi715 – 7151MagnesiumBy similarity
    Metal bindingi719 – 7191MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sodium:potassium-exchanging ATPase activity Source: UniProtKB

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. negative regulation of glucocorticoid biosynthetic process Source: Ensembl
    3. negative regulation of heart contraction Source: Ensembl
    4. positive regulation of heart contraction Source: Ensembl
    5. positive regulation of striated muscle contraction Source: Ensembl
    6. regulation of blood pressure Source: Ensembl
    7. regulation of sodium ion transport Source: UniProtKB
    8. regulation of the force of heart contraction Source: Ensembl
    9. response to drug Source: Ensembl
    10. sodium ion transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha-1 (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha-1 subunit
    Alternative name(s):
    Sodium pump subunit alpha-1
    Gene namesi
    Name:ATP1A1
    OrganismiOvis aries (Sheep)
    Taxonomic identifieri9940 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
    ProteomesiUP000002356: Chromosome 1

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. integral component of membrane Source: UniProtKB
    3. melanosome Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB
    5. T-tubule Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 55By similarityPRO_0000002491
    Chaini6 – 10211016Sodium/potassium-transporting ATPase subunit alpha-1PRO_0000002492Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91N6-acetyllysineBy similarity
    Modified residuei10 – 101PhosphotyrosineBy similarity
    Modified residuei16 – 161Phosphoserine; by PKCBy similarity
    Modified residuei21 – 211N6-acetyllysineBy similarity
    Modified residuei258 – 2581PhosphotyrosineBy similarity
    Modified residuei540 – 5401PhosphotyrosineBy similarity
    Modified residuei941 – 9411Phosphoserine; by PKABy similarity

    Post-translational modificationi

    Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-941 by PKA modulates the response of ATP1A1 to PKC By similarity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with SIK1 By similarity. Interacts with SLC35G1 and STIM1 By similarity. Composed of three subunits: alpha (catalytic), beta and gamma.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP04074.
    SMRiP04074. Positions 24-1021.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini6 – 8580CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini107 – 12923ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini151 – 286136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini307 – 31812ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini337 – 770434CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini791 – 80010ExtracellularSequence Analysis
    Topological domaini822 – 84120CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini865 – 91652ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini937 – 94913CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini969 – 98315ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1005 – 102117CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei86 – 10621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei287 – 30620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 33618HelicalSequence AnalysisAdd
    BLAST
    Transmembranei771 – 79020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei801 – 82121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei842 – 86423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei917 – 93620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei950 – 96819HelicalSequence AnalysisAdd
    BLAST
    Transmembranei984 – 100421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 823Phosphoinositide-3 kinase bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00560000076866.
    HOVERGENiHBG004298.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04074-1 [UniParc]FASTAAdd to Basket

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    MGKGVGRDKY EPAAVSEHGD KKKAKKERDM DELKKEVSMD DHKLSLDELH     50
    RKYGTDLNRG LTTARAAEIL ARDGPNALTP PPTTPEWVKF CRQLFGGFSM 100
    LLWIGAVLCF LAYGIQAATE EEPQNDNLYL GVVLSAVVII TGCFSYYQEA 150
    KSSKIMESFK NMVPQQALVI RNGEKMSINA EEVVVGDLVE VKGGDRIPAD 200
    LRIISANGCK VDNSSLTGES EPQTRSPDFT NENPLETRNI AFFSTNCVEG 250
    TARGIVVYTG DRTVMGRIAT LASGLEGGQT PIAAEIEHFI HIITGVAVFL 300
    GVSFFILSLI LEYTWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA 350
    RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT 400
    TENQSGVSFD KTSATWLALS RIAGLCNRAV FQANQDNLPI LKRAVAGDAS 450
    ESALLKCIEV CCGSVKEMRE RYAKIVEIPF NSTNKYQLSI HKNANAGEPR 500
    HLLVMKGAPE RILDRCSSIL IHGKEQPLDE ELKDAFQNAY LELGGLGERV 550
    LGFCHLMLPD EQFPEGFQFD TDDVNFPVDN LCFVGLISMI DPPRAAVPDA 600
    VGKCRSAGIK VIMVTGDHPI TAKAIAKGVG IISEGNETVE DIAARLNIPV 650
    SQVNPRDARA CVVHGSDLKD MTPEQLDDIL KYHTEIVFAR TSPQQKLIIV 700
    EGCQRQGAIV AVTGDGVNDS PALKKADIGV AMGIAGSDVS KQAADMILLD 750
    DNFASIVTGV EEGRLIFDNL KKSIAYTLTS NIPEITPFLI FIIANIPLPL 800
    GTVTILCIDL GTDMVPAISL AYEQAESDIM KRQPRNPQTD KLVNERLISM 850
    AYGQIGMIQA LGGFFTYFVI MAENGFLPNH LLGIRVTWDD RWINDVEDSY 900
    GQQWTYEQRK IVEFTCHTAF FVSIVVVQWA DLVICKTRRN SVFQQGMKNK 950
    ILIFGLFEET ALAAFLSYCP GMGVALRMYP LKPTWWFCAF PYSLLIFVYD 1000
    EVRKLIIRRR PGGWVEKETY Y 1021
    Length:1,021
    Mass (Da):112,658
    Last modified:November 1, 1986 - v1
    Checksum:i0D493D95BD9D4312
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02813 mRNA. Translation: CAA26581.1.
    X02813 mRNA. Translation: CAA26582.1.
    PIRiA01074. PWSHNA.
    RefSeqiNP_001009360.1. NM_001009360.1.
    UniGeneiOar.424.

    Genome annotation databases

    EnsembliENSOART00000022009; ENSOARP00000021706; ENSOARG00000020213.
    GeneIDi443381.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02813 mRNA. Translation: CAA26581.1 .
    X02813 mRNA. Translation: CAA26582.1 .
    PIRi A01074. PWSHNA.
    RefSeqi NP_001009360.1. NM_001009360.1.
    UniGenei Oar.424.

    3D structure databases

    ProteinModelPortali P04074.
    SMRi P04074. Positions 24-1021.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSOART00000022009 ; ENSOARP00000021706 ; ENSOARG00000020213 .
    GeneIDi 443381.

    Organism-specific databases

    CTDi 476.

    Phylogenomic databases

    GeneTreei ENSGT00560000076866.
    HOVERGENi HBG004298.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA."
      Shull G.E., Schwartz A., Lingrel J.B.
      Nature 316:691-695(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "Lysine 480 is an essential residue in the putative ATP site of lamb kidney (Na,K)-ATPase. Identification of the pyridoxal 5'-diphospho-5'-adenosine and pyridoxal phosphate reactive residue."
      Hinz H.R., Kirley T.L.
      J. Biol. Chem. 265:10260-10265(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 475-492.

    Entry informationi

    Entry nameiAT1A1_SHEEP
    AccessioniPrimary (citable) accession number: P04074
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1986
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3