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Protein

Gastricsin

Gene

Pgc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes a variety of proteins.

Catalytic activityi

More restricted specificity than pepsin A, but shows preferential cleavage at Tyr-|-Xaa bonds. High activity on hemoglobin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941
Active sitei280 – 2801

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiA01.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Gastricsin (EC:3.4.23.3)
Alternative name(s):
Pepsinogen C
Gene namesi
Name:Pgc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi3943. Pgc.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2152.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Propeptidei17 – 6246Activation peptidePRO_0000026069Add
BLAST
Chaini63 – 392330GastricsinPRO_0000026070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi107 ↔ 112By similarity
Disulfide bondi270 ↔ 275By similarity
Disulfide bondi314 ↔ 347By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP04073.
PRIDEiP04073.

PTM databases

iPTMnetiP04073.

Expressioni

Gene expression databases

GenevisibleiP04073. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019650.

Chemistry

BindingDBiP04073.

Structurei

3D structure databases

ProteinModelPortaliP04073.
SMRiP04073. Positions 17-59, 63-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 389314Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP04073.
KOiK01377.
OMAiASGWCSE.
OrthoDBiEOG7HQN88.
PhylomeDBiP04073.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04073-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWMVVALLC LPLLEASLLR VPLRKMKSIR ETMKEQGVLK DFLKTHKYDP
60 70 80 90 100
GQKYHFGNFG DYSVLYEPMA YMDASYFGEI SIGTPPQNFL VLFDTGSSNL
110 120 130 140 150
WVSSVYCQSE ACTTHARFNP SKSSTYYTEG QTFSLQYGTG SLTGFFGYDT
160 170 180 190 200
LTVQSIQVPN QEFGLSENEP GTNFVYAQFD GIMGLAYPGL SSGGATTALQ
210 220 230 240 250
GMLGEGALSQ PLFGVYLGSQ QGSNGGQIVF GGVDKNLYTG EITWVPVTQE
260 270 280 290 300
LYWQITIDDF LIGDQASGWC SSQGCQGIVD TGTSLLVMPA QYLSELLQTI
310 320 330 340 350
GAQEGEYGEY FVSCDSVSSL PTLSFVLNGV QFPLSPSSYI IQEDNFCMVG
360 370 380 390
LESISLTSES GQPLWILGDV FLRSYYAIFD MGNNKVGLAT SV
Length:392
Mass (Da):42,833
Last modified:November 1, 1986 - v1
Checksum:i092A5EAF2783EDD1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311E → Q AA sequence (PubMed:6743670).Curated
Sequence conflicti103 – 1031S → A AA sequence (PubMed:6743670).Curated
Sequence conflicti109 – 1091S → L AA sequence (PubMed:6743670).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04644 mRNA. Translation: CAA28305.1.
M25993
, M25985, M25986, M25987, M25988, M25989, M25990, M25991, M25992 Genomic DNA. Translation: AAA41827.1.
PIRiA33510. A24608.
RefSeqiNP_579818.1. NM_133284.2.
UniGeneiRn.9735.

Genome annotation databases

EnsembliENSRNOT00000019650; ENSRNOP00000019650; ENSRNOG00000014492.
GeneIDi24864.
KEGGirno:24864.
UCSCiRGD:3943. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04644 mRNA. Translation: CAA28305.1.
M25993
, M25985, M25986, M25987, M25988, M25989, M25990, M25991, M25992 Genomic DNA. Translation: AAA41827.1.
PIRiA33510. A24608.
RefSeqiNP_579818.1. NM_133284.2.
UniGeneiRn.9735.

3D structure databases

ProteinModelPortaliP04073.
SMRiP04073. Positions 17-59, 63-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019650.

Chemistry

BindingDBiP04073.
ChEMBLiCHEMBL2152.

Protein family/group databases

MEROPSiA01.003.

PTM databases

iPTMnetiP04073.

Proteomic databases

PaxDbiP04073.
PRIDEiP04073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019650; ENSRNOP00000019650; ENSRNOG00000014492.
GeneIDi24864.
KEGGirno:24864.
UCSCiRGD:3943. rat.

Organism-specific databases

CTDi5225.
RGDi3943. Pgc.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiP04073.
KOiK01377.
OMAiASGWCSE.
OrthoDBiEOG7HQN88.
PhylomeDBiP04073.
TreeFamiTF314990.

Miscellaneous databases

PROiP04073.

Gene expression databases

GenevisibleiP04073. RN.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of a nearly full-length cDNA coding for pepsinogen of rat gastric mucosa."
    Ichihara Y., Sogawa K., Morohashi K., Fujii-Kuriyama Y., Takahashi K.
    Eur. J. Biochem. 161:7-12(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
  2. "Primary structure and transcriptional regulation of rat pepsinogen C gene."
    Ishihara T., Ichihara Y., Hayano T., Katsura I., Sogawa K., Fujii-Kuriyama Y., Takahashi K.
    J. Biol. Chem. 264:10193-10199(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
  3. Cited for: PROTEIN SEQUENCE OF 16-112.
    Strain: Wistar.

Entry informationi

Entry nameiPEPC_RAT
AccessioniPrimary (citable) accession number: P04073
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.