P04073 (PEPC_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastricsin
EC=3.4.23.3

Alternative name(s):
Pepsinogen C

Gene names

Name:

Pgc

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes a variety of proteins.
Catalytic activity	More restricted specificity than pepsin A, but shows preferential cleavage at Tyr-|-Xaa bonds. High activity on hemoglobin.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: Compara
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.1
	Propeptide	17 – 62	46	Activation peptide		PRO_0000026069
	Chain	63 – 392	330	Gastricsin		PRO_0000026070
Sites
	Active site	94	1
	Active site	280	1
Amino acid modifications
	Disulfide bond	107 ↔ 112		By similarity
	Disulfide bond	270 ↔ 275		By similarity
	Disulfide bond	314 ↔ 347		By similarity
Experimental info
	Sequence conflict	31	1	E → Q AA sequence Ref.3
	Sequence conflict	103	1	S → A AA sequence Ref.3
	Sequence conflict	109	1	S → L AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P04073 [UniParc]. Last modified November 1, 1986. Version 1. Checksum: 092A5EAF2783EDD1	FASTA	392	42,833
	10 20 30 40 50 60 MKWMVVALLC LPLLEASLLR VPLRKMKSIR ETMKEQGVLK DFLKTHKYDP GQKYHFGNFG 70 80 90 100 110 120 DYSVLYEPMA YMDASYFGEI SIGTPPQNFL VLFDTGSSNL WVSSVYCQSE ACTTHARFNP 130 140 150 160 170 180 SKSSTYYTEG QTFSLQYGTG SLTGFFGYDT LTVQSIQVPN QEFGLSENEP GTNFVYAQFD 190 200 210 220 230 240 GIMGLAYPGL SSGGATTALQ GMLGEGALSQ PLFGVYLGSQ QGSNGGQIVF GGVDKNLYTG 250 260 270 280 290 300 EITWVPVTQE LYWQITIDDF LIGDQASGWC SSQGCQGIVD TGTSLLVMPA QYLSELLQTI 310 320 330 340 350 360 GAQEGEYGEY FVSCDSVSSL PTLSFVLNGV QFPLSPSSYI IQEDNFCMVG LESISLTSES 370 380 390 GQPLWILGDV FLRSYYAIFD MGNNKVGLAT SV

References

[1]	"Nucleotide sequence of a nearly full-length cDNA coding for pepsinogen of rat gastric mucosa." Ichihara Y., Sogawa K., Morohashi K., Fujii-Kuriyama Y., Takahashi K. Eur. J. Biochem. 161:7-12(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[2]	"Primary structure and transcriptional regulation of rat pepsinogen C gene." Ishihara T., Ichihara Y., Hayano T., Katsura I., Sogawa K., Fujii-Kuriyama Y., Takahashi K. J. Biol. Chem. 264:10193-10199(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar.
[3]	"The N-terminal sequence of rat pepsinogen." Arai K.M., Muto N., Tani S., Akahane K. Biochim. Biophys. Acta 788:256-261(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-112. Strain: Wistar.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04644 mRNA. Translation: CAA28305.1. M25993 , M25985, M25986, M25987, M25988, M25989, M25990, M25991, M25992 Genomic DNA. Translation: AAA41827.1.
IPI	IPI00324445.
PIR	A24608. A33510.
RefSeq	NP_579818.1. NM_133284.2.
UniGene	Rn.9735.
3D structure databases
ProteinModelPortal	P04073.
SMR	P04073. Positions 17-59, 63-391.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000019650.
Protein family/group databases
MEROPS	A01.003.
Proteomic databases
PaxDb	P04073.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000019650; ENSRNOP00000019650; ENSRNOG00000014492.
GeneID	24864.
KEGG	rno:24864.
UCSC	RGD:3943. rat.
Organism-specific databases
CTD	5225.
RGD	3943. Pgc.
Phylogenomic databases
eggNOG	NOG248684.
GeneTree	ENSGT00700000104165.
HOGENOM	HOG000197681.
HOVERGEN	HBG000482.
InParanoid	P04073.
KO	K01377.
OMA	ASGWCSE.
Gene expression databases
ArrayExpress	P04073.
Genevestigator	P04073.
GermOnline	ENSRNOG00000014492. Rattus norvegicus.
Family and domain databases
Gene3D	2.40.70.10. 2 hits.
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR012848. Propep_A1. [Graphical view]
PANTHER	PTHR13683. PTHR13683. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P04073.
ChEMBL	CHEMBL2152.
NextBio	604678.

Entry information

Entry name

PEPC_RAT

Accession

Primary (citable) accession number: P04073

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	November 1, 1986
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program