Reviewed, UniProtKB/Swiss-Prot P04071 (K1B16_MOUSE)

Names and origin

Protein names

Recommended name:
    Kallikrein 1-related peptidase b16
    EC=3.4.21.54
Alternative name(s):
    Gamma-renin, submandibular gland
      Short name=mGK-16

Gene names

Name:	Klk1b16
Synonyms:	Klk-16, Klk16

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Cleavage of the Leu-|-Leu bond in synthetic tetradecapeptide renin substrate, to produce angiotensin I, but not active on natural angiotensinogen. Also hydrolyzes Bz-Arg-p-nitroanilide.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 24	6	Activation peptide Ref.4		PRO_0000027985
	Chain	25 – 261	237	Kallikrein 1-related peptidase b16		PRO_0000027986
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	213	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 173		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	152 ↔ 219		By similarity
	Disulfide bond	184 ↔ 198		By similarity
	Disulfide bond	209 ↔ 234		By similarity
Experimental info
	Sequence conflict	45	1	H → A AA sequence Ref.4
	Sequence conflict	48 – 49	2	HI → YL AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P04071-1 [UniParc]. Last modified July 1, 1989. Version 2. Checksum: 53D8984BF41E0F3D	FASTA	261	28,722
	10 20 30 40 50 60 MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWQV AVYYHKEHIC GGVLLDRNWV 70 80 90 100 110 120 LTAAHCYVDE CEVWLGKNQL FQEEPSAQNR LVSKSFPHPG FNMTLLTFEK LPPGADFSND 130 140 150 160 170 180 LMLLRLSKPA DITDVVKPID LPTKEPKLDS TCLVSGWGSI TPTKWQKPDD LQCMFTKLLP 190 200 210 220 230 240 NENCAKAYLL KVTDVMLCTI EMGEDKGPCV GDSGGPLICD GVLQGTVSIG PDPCGIPGVS 250 260 AIYTNLVKFN SWIKDTMMKN A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression of mouse gamma-renin." Drinkwater C.C., Evans B.A., Richards R.I. J. Biol. Chem. 263:8565-8568(1988) [PubMed: 3288617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[3]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed: 3036794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-54 AND 70-122.
[4]	"Purification and properties of renin and gamma-renin from the mouse submaxillary gland." Poe M., Wu J.K., Florance J.R., Rodkey J.A., Bennett C.D., Hoogsteen K. J. Biol. Chem. 258:2209-2216(1983) [PubMed: 6337154] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-64 AND 165-184. Strain: Swiss Webster.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03877 mRNA. Translation: AAA40049.1. BC012237 mRNA. Translation: AAH12237.1. M18594 Genomic DNA. Translation: AAA39357.1. M18615 Genomic DNA. Translation: AAA39358.1.
IPI	IPI00113575.
PIR	A28062.
RefSeq	NP_032480.1.
UniGene	Mm.440887
3D structure databases
HSSP	HSSP built from PDB template 1AO5 based on UniProtKB P36368.
SMR	P04071. Positions 25-261.
ModBase	Search...
Protein family/group databases
MEROPS	S01.163.
Proteomic databases
PRIDE	P04071.
Genome annotation databases
Ensembl	ENSMUSG00000038968. Mus musculus. [Contig view]
GeneID	16615.
KEGG	mmu:16615.
Organism-specific databases
MGI	MGI:891982. Klk1b16.
Phylogenomic databases
HOVERGEN	P04071.
OMA	P04071. CKSEDHA.
Enzyme and pathway databases
BRENDA	3.4.21.54. 244.
Gene expression databases
ArrayExpress	P04071.
Bgee	P04071.
CleanEx	MM_KLK1B16.
GermOnline	ENSMUSG00000038968. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290207.
SOURCE	Search...

Entry information

Entry name

K1B16_MOUSE

Accession

Primary (citable) accession number: P04071

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)