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Protein

Epoxide hydrolase 1

Gene

EPHX1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids.By similarity

Catalytic activityi

Cis-stilbene oxide + H2O = (1R,2R)-1,2-diphenylethane-1,2-diol.By similarity
1-(4-methoxyphenyl)-N-methyl-N-((3-methyloxetan-3-yl)methyl)methanamine + H2O = 2-((((4-methoxyphenyl)methyl)(methyl)amino)methyl)-2-methylpropane-1,3-diol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei226NucleophileBy similarity1
Active sitei374Proton donorBy similarity1
Active sitei431Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAromatic hydrocarbons catabolism, Detoxification

Protein family/group databases

ESTHERirabit-hyep Epoxide_hydrolase
MEROPSiS33.971

Names & Taxonomyi

Protein namesi
Recommended name:
Epoxide hydrolase 1 (EC:3.3.2.9By similarity)
Alternative name(s):
Epoxide hydratase
Microsomal epoxide hydrolase
Short name:
mEHCurated
Gene namesi
Name:EPHX1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini22 – 455CytoplasmicBy similarityAdd BLAST434

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000808581 – 455Epoxide hydrolase 1Add BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295Dimethylated arginine; alternateBy similarity1
Modified residuei295Omega-N-methylated arginine; alternateBy similarity1

Keywords - PTMi

Methylation

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000010259

Structurei

3D structure databases

ProteinModelPortaliP04068
SMRiP04068
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2565 Eukaryota
COG0596 LUCA
HOGENOMiHOG000132894
HOVERGENiHBG002366
InParanoidiP04068
KOiK01253

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000639 Epox_hydrolase-like
IPR010497 Epoxide_hydro_N
IPR016292 Epoxide_hydrolase
PfamiView protein in Pfam
PF06441 EHN, 1 hit
PIRSFiPIRSF001112 Epoxide_hydrolase, 1 hit
PRINTSiPR00412 EPOXHYDRLASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

P04068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLELLLASV LGFVIYWFVS GDKEESLPLE DGWWGPGSRP VGLEDESIRP
60 70 80 90 100
FKVETSDEEI NDLHQRIDRI RLTPPLENSR FHYGFNSNYL KKILSYWRHE
110 120 130 140 150
FDWKKQVEIL NSYPHFKTKI EGLDIHFIHV KPPQVPPGRT PKPLLMVHGW
160 170 180 190 200
PGSFFEFYKI IPLLTDPKSH GLSDEHIFEV ICPSIPGYGF SQASSKKGFN
210 220 230 240 250
SVSTARIFYK LMLRLGFQEF YIQGGDWGAL VCTNMAQLVP SHVKGLHLNM
260 270 280 290 300
ALILRNHYTL TLLLGRRIGG LLGYTERDME LLYPFKEKVF YSLMRESGYM
310 320 330 340 350
HIRATKPDTV GCALNDSPVG LAAYILEKFS TWTNSEFRDL EDGGLERKFS
360 370 380 390 400
LQDLLTNIMI YWTTGSIVSS QRYYKENLGQ GFMAHKHERL KVHVPTGFAA
410 420 430 440 450
FPCEIMHVPE KWVRTKYPQL ISYSYMPRGG HFAAFEEPEL LARDICKFVG

LVERQ
Length:455
Mass (Da):52,512
Last modified:August 1, 1990 - v2
Checksum:i8A1F556275389AD2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68 – 70Missing AA sequence (PubMed:6693396).Curated3
Sequence conflicti113Y → L AA sequence (PubMed:6693396).Curated1
Sequence conflicti201 – 203SVS → NVV AA sequence (PubMed:6693396).Curated3
Sequence conflicti212M → RISSY AA sequence (PubMed:6693396).Curated1
Sequence conflicti248 – 249LN → DL AA sequence (PubMed:6693396).Curated2
Sequence conflicti303R → Q AA sequence (PubMed:6693396).Curated1
Sequence conflicti366 – 371SIVSSQ → WIRTHY AA sequence (PubMed:6693396).Curated6
Sequence conflicti455Missing AA sequence (PubMed:6693396).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21496 mRNA Translation: AAA31392.1
PIRiS04342 YXRBH
RefSeqiNP_001075744.1, NM_001082275.1
UniGeneiOcu.6248

Genome annotation databases

GeneIDi100009104
KEGGiocu:100009104

Similar proteinsi

Entry informationi

Entry nameiHYEP_RABIT
AccessioniPrimary (citable) accession number: P04068
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: August 1, 1990
Last modified: March 28, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health