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P04068

- HYEP_RABIT

UniProt

P04068 - HYEP_RABIT

Protein

Epoxide hydrolase 1

Gene

EPHX1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.

    Catalytic activityi

    Cis-stilbene oxide + H2O = (+)-(1R,2R)-1,2-diphenylethane-1,2-diol.

    GO - Molecular functioni

    1. cis-stilbene-oxide hydrolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW
    2. response to toxic substance Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism, Detoxification

    Protein family/group databases

    MEROPSiS33.971.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epoxide hydrolase 1 (EC:3.3.2.9)
    Alternative name(s):
    Epoxide hydratase
    Microsomal epoxide hydrolase
    Gene namesi
    Name:EPHX1
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 455455Epoxide hydrolase 1PRO_0000080858Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei295 – 2951Omega-N-methylated arginineBy similarity

    Keywords - PTMi

    Methylation

    Interactioni

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000010259.

    Structurei

    3D structure databases

    ProteinModelPortaliP04068.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2 – 2019Helical; Signal-anchorSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S33 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0596.
    HOGENOMiHOG000132894.
    HOVERGENiHBG002366.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR010497. Epoxide_hydro_N.
    IPR016292. Epoxide_hydrolase.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    PF06441. EHN. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001112. Epoxide_hydrolase. 1 hit.
    PRINTSiPR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P04068-1 [UniParc]FASTAAdd to Basket

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    MLLELLLASV LGFVIYWFVS GDKEESLPLE DGWWGPGSRP VGLEDESIRP    50
    FKVETSDEEI NDLHQRIDRI RLTPPLENSR FHYGFNSNYL KKILSYWRHE 100
    FDWKKQVEIL NSYPHFKTKI EGLDIHFIHV KPPQVPPGRT PKPLLMVHGW 150
    PGSFFEFYKI IPLLTDPKSH GLSDEHIFEV ICPSIPGYGF SQASSKKGFN 200
    SVSTARIFYK LMLRLGFQEF YIQGGDWGAL VCTNMAQLVP SHVKGLHLNM 250
    ALILRNHYTL TLLLGRRIGG LLGYTERDME LLYPFKEKVF YSLMRESGYM 300
    HIRATKPDTV GCALNDSPVG LAAYILEKFS TWTNSEFRDL EDGGLERKFS 350
    LQDLLTNIMI YWTTGSIVSS QRYYKENLGQ GFMAHKHERL KVHVPTGFAA 400
    FPCEIMHVPE KWVRTKYPQL ISYSYMPRGG HFAAFEEPEL LARDICKFVG 450
    LVERQ 455
    Length:455
    Mass (Da):52,512
    Last modified:August 1, 1990 - v2
    Checksum:i8A1F556275389AD2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 703Missing AA sequence (PubMed:6693396)Curated
    Sequence conflicti113 – 1131Y → L AA sequence (PubMed:6693396)Curated
    Sequence conflicti201 – 2033SVS → NVV AA sequence (PubMed:6693396)Curated
    Sequence conflicti212 – 2121M → RISSY AA sequence (PubMed:6693396)Curated
    Sequence conflicti248 – 2492LN → DL AA sequence (PubMed:6693396)Curated
    Sequence conflicti303 – 3031R → Q AA sequence (PubMed:6693396)Curated
    Sequence conflicti366 – 3716SIVSSQ → WIRTHY AA sequence (PubMed:6693396)Curated
    Sequence conflicti455 – 4551Missing AA sequence (PubMed:6693396)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21496 mRNA. Translation: AAA31392.1.
    PIRiS04342. YXRBH.
    RefSeqiNP_001075744.1. NM_001082275.1.
    UniGeneiOcu.6248.

    Genome annotation databases

    GeneIDi100009104.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21496 mRNA. Translation: AAA31392.1 .
    PIRi S04342. YXRBH.
    RefSeqi NP_001075744.1. NM_001082275.1.
    UniGenei Ocu.6248.

    3D structure databases

    ProteinModelPortali P04068.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000010259.

    Protein family/group databases

    MEROPSi S33.971.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009104.

    Organism-specific databases

    CTDi 2052.

    Phylogenomic databases

    eggNOGi COG0596.
    HOGENOMi HOG000132894.
    HOVERGENi HBG002366.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR010497. Epoxide_hydro_N.
    IPR016292. Epoxide_hydrolase.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    PF06441. EHN. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001112. Epoxide_hydrolase. 1 hit.
    PRINTSi PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rabbit microsomal epoxide hydrolase: isolation and characterization of the xenobiotic metabolizing enzyme cDNA."
      Hassett C., Turnblom S.M., Deangeles A., Omiecinski C.J.
      Arch. Biochem. Biophys. 271:380-389(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The covalent structure of hepatic microsomal epoxide hydrolase. II. The complete amino acid sequence."
      Heinemann F.S., Ozols J.
      J. Biol. Chem. 259:797-804(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Strain: New Zealand white.
      Tissue: Liver.

    Entry informationi

    Entry nameiHYEP_RABIT
    AccessioniPrimary (citable) accession number: P04068
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3