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P04065 (AMYH_YEASX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucoamylase S1

EC=3.2.1.3
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAI
Glucan 1,4-alpha-glucosidase
Gene names
Name:STA1
Synonyms:DEX2, MAL5
OrganismSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length767 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

Sequence caution

The sequence CAA26487.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionglucan 1,4-alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 767746Glucoamylase S1
PRO_0000001477

Regions

Region348 – 691344H subunit
Region692 – 76776Y subunit
Compositional bias22 – 347326Ser/Thr-rich

Sites

Active site5181Proton acceptor By similarity
Active site5211Proton donor By similarity
Binding site4551Substrate By similarity

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation3081N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation4141N-linked (GlcNAc...) Potential
Glycosylation4231N-linked (GlcNAc...) Potential
Glycosylation4341N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Glycosylation5461N-linked (GlcNAc...) Potential
Glycosylation6451N-linked (GlcNAc...) Potential
Glycosylation6501N-linked (GlcNAc...) Potential
Glycosylation7201N-linked (GlcNAc...) Potential
Glycosylation7411N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P04065 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: A5F29E2427EDB593

FASTA76782,489
        10         20         30         40         50         60 
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT ESFSTGTTVT 

        70         80         90        100        110        120 
PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI TTTVCSTGTN SAGETTSGCS 

       130        140        150        160        170        180 
PKTITTTVPC STSPSETASE STTTSPTTPV TTVVSTTVVT TEYSTSTKQG GEITTTFVTK 

       190        200        210        220        230        240 
NIPTTYLTTI APTSSVTTVT NFTPTTITTT VCSTGTNSAG ETTSGCSPKT VTTTVPCSTG 

       250        260        270        280        290        300 
TGEYTTEATA PVTTAVTTTV VTTESSTGTN SAGKTTTSYT TKSVPTTYVF DFGKGILDQS 

       310        320        330        340        350        360 
CGGVFSNNGS SQVQLRDVVL MNGTVVYDSN GAWDSSALEE WLQRQKKVSI ERIFENIGPS 

       370        380        390        400        410        420 
AVYPSILPGV VIASPSQTHP DYFYQWIRDS ALTINSIVSH SADPAIETLL QYLNVSFHLQ 

       430        440        450        460        470        480 
RTNNTLGAGI GYTNDTVALG DPKWNVDNTA FTEPWGRPQN DGPALRSIAI LKIIDYIKQS 

       490        500        510        520        530        540 
GTDLGAKYPF QSTADIFDDI VRWDLRFIID HWNSSGFDLW EEVNGMHFFT LLVQLSAVDR 

       550        560        570        580        590        600 
SLSYFNASER SSPFVEELRQ TRRDISKFLV DPANGFINGK YNYIVETPMI ADTLRSGLDI 

       610        620        630        640        650        660 
STLLAANTVH DAPSASHLPF DINDPAVLNT LHHLMLHMRS IYPINDSSKN ATGIALGRYP 

       670        680        690        700        710        720 
EDVYDGYGVG EGNPWVLATC AASTTLYQLI YRHISEQHDL VVPMNNDCSN AFWSELVFSN 

       730        740        750        760 
LTTLGNDEGY LILEFNTPAF NQTIQKIFQL ADSFLVKLKA TWEQTGN 

« Hide

References

[1]"Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus."
Yamashita I., Suzuki K., Fukui S.
J. Bacteriol. 161:567-573(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Diastaticus / ATCC 60709 / 5106-9A.
[2]"Proteolytic processing of glucoamylase in the yeast Saccharomyces cerevisiae."
Yamashita I., Suzuki K., Sakuzo F.
Agric. Biol. Chem. 50:475-482(1986) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
[3]"Upstream regions of the yeast glucoamylase gene which are required for efficient transcription."
Shima H., Inui M., Akada R., Yamashita I.
Agric. Biol. Chem. 53:749-755(1989)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02649 Genomic DNA. Translation: CAA26487.1. Different initiation.
D00428 Genomic DNA. Translation: BAA00332.1.
PIRALBYG. A21896.

3D structure databases

ProteinModelPortalP04065.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH15. Glycoside Hydrolase Family 15.

Proteomic databases

PRIDEP04065.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG3387.

Enzyme and pathway databases

BRENDA3.2.1.3. 8373.

Gene expression databases

GenevestigatorP04065.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSPR00736. GLHYDRLASE15.
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMYH_YEASX
AccessionPrimary (citable) accession number: P04065
Secondary accession number(s): Q92314
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1990
Last modified: October 16, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries