Glucoamylase S1 precursor - Saccharomyces cerevisiae (Baker's yeast)

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P04065 (AMYH_YEASX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glucoamylase S1
EC=3.2.1.3

Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAI
Glucan 1,4-alpha-glucosidase

Gene names

Name:	STA1
Synonyms:	DEX2, MAL5

Organism

Saccharomyces cerevisiae (Baker's yeast)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 15 family.
Sequence caution	The sequence CAA26487.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glucan 1,4-alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

21

Chain

746

Glucoamylase S1

PRO_0000001477

Regions

Region

344

H subunit

Region

76

Y subunit

Compositional bias

326

Ser/Thr-rich

Sites

Active site

1

Proton acceptor By similarity

Active site

1

Proton donor By similarity

Binding site

1

Substrate By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P04065 [UniParc].

Last modified November 1, 1990. Version 2.
Checksum: A5F29E2427EDB593
Show »

767

82,489

        10         20         30         40         50         60 
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT ESFSTGTTVT 

        70         80         90        100        110        120 
PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI TTTVCSTGTN SAGETTSGCS 

       130        140        150        160        170        180 
PKTITTTVPC STSPSETASE STTTSPTTPV TTVVSTTVVT TEYSTSTKQG GEITTTFVTK 

       190        200        210        220        230        240 
NIPTTYLTTI APTSSVTTVT NFTPTTITTT VCSTGTNSAG ETTSGCSPKT VTTTVPCSTG 

       250        260        270        280        290        300 
TGEYTTEATA PVTTAVTTTV VTTESSTGTN SAGKTTTSYT TKSVPTTYVF DFGKGILDQS 

       310        320        330        340        350        360 
CGGVFSNNGS SQVQLRDVVL MNGTVVYDSN GAWDSSALEE WLQRQKKVSI ERIFENIGPS 

       370        380        390        400        410        420 
AVYPSILPGV VIASPSQTHP DYFYQWIRDS ALTINSIVSH SADPAIETLL QYLNVSFHLQ 

       430        440        450        460        470        480 
RTNNTLGAGI GYTNDTVALG DPKWNVDNTA FTEPWGRPQN DGPALRSIAI LKIIDYIKQS 

       490        500        510        520        530        540 
GTDLGAKYPF QSTADIFDDI VRWDLRFIID HWNSSGFDLW EEVNGMHFFT LLVQLSAVDR 

       550        560        570        580        590        600 
SLSYFNASER SSPFVEELRQ TRRDISKFLV DPANGFINGK YNYIVETPMI ADTLRSGLDI 

       610        620        630        640        650        660 
STLLAANTVH DAPSASHLPF DINDPAVLNT LHHLMLHMRS IYPINDSSKN ATGIALGRYP 

       670        680        690        700        710        720 
EDVYDGYGVG EGNPWVLATC AASTTLYQLI YRHISEQHDL VVPMNNDCSN AFWSELVFSN 

       730        740        750        760 
LTTLGNDEGY LILEFNTPAF NQTIQKIFQL ADSFLVKLKA TWEQTGN

References

[1]	"Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus." Yamashita I., Suzuki K., Fukui S. J. Bacteriol. 161:567-573(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Diastaticus / ATCC 60709 / 5106-9A.
[2]	"Proteolytic processing of glucoamylase in the yeast Saccharomyces cerevisiae." Yamashita I., Suzuki K., Sakuzo F. Agric. Biol. Chem. 50:475-482(1986) [AGRICOLA] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
[3]	"Upstream regions of the yeast glucoamylase gene which are required for efficient transcription." Shima H., Inui M., Akada R., Yamashita I. Agric. Biol. Chem. 53:749-755(1989) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02649 Genomic DNA. Translation: CAA26487.1. Different initiation. D00428 Genomic DNA. Translation: BAA00332.1.
PIR	ALBYG. A21896.
3D structure databases
ProteinModelPortal	P04065.
ModBase	Search...
Protein family/group databases
CAZy	GH15. Glycoside Hydrolase Family 15.
Proteomic databases
PRIDE	P04065.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG3387.
Enzyme and pathway databases
BRENDA	3.2.1.3. 8373.
Gene expression databases
Genevestigator	P04065.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR000165. Glucoamylase. IPR011613. Glyco_hydro_15. [Graphical view]
Pfam	PF00723. Glyco_hydro_15. 1 hit. [Graphical view]
PRINTS	PR00736. GLHYDRLASE15.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00820. GLUCOAMYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYH_YEASX

Accession

Primary (citable) accession number: P04065
Secondary accession number(s): Q92314

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents