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Protein

Glucoamylase S1

Gene

STA1

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei455SubstrateBy similarity1
Active sitei518Proton acceptorPROSITE-ProRule annotation1
Active sitei521Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.3. 5495.

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase S1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAI
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:STA1
Synonyms:DEX2, MAL5
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000000147722 – 767Glucoamylase S1Add BLAST746

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi322N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi414N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi423N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi434N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi513N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi546N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi645N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi650N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi720N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi741N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP04065.
PRIDEiP04065.

Structurei

3D structure databases

ProteinModelPortaliP04065.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni348 – 691H subunitAdd BLAST344
Regioni692 – 767Y subunitAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 347Ser/Thr-richAdd BLAST326

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGG8. Eukaryota.
COG3387. LUCA.

Family and domain databases

InterProiView protein in InterPro
IPR008928. 6-hairpin_glycosidase-like.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15/PHK.
PfamiView protein in Pfam
PF00723. Glyco_hydro_15. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiView protein in PROSITE
PS00820. GLUCOAMYLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT
60 70 80 90 100
ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI
110 120 130 140 150
TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV
160 170 180 190 200
TTVVSTTVVT TEYSTSTKQG GEITTTFVTK NIPTTYLTTI APTSSVTTVT
210 220 230 240 250
NFTPTTITTT VCSTGTNSAG ETTSGCSPKT VTTTVPCSTG TGEYTTEATA
260 270 280 290 300
PVTTAVTTTV VTTESSTGTN SAGKTTTSYT TKSVPTTYVF DFGKGILDQS
310 320 330 340 350
CGGVFSNNGS SQVQLRDVVL MNGTVVYDSN GAWDSSALEE WLQRQKKVSI
360 370 380 390 400
ERIFENIGPS AVYPSILPGV VIASPSQTHP DYFYQWIRDS ALTINSIVSH
410 420 430 440 450
SADPAIETLL QYLNVSFHLQ RTNNTLGAGI GYTNDTVALG DPKWNVDNTA
460 470 480 490 500
FTEPWGRPQN DGPALRSIAI LKIIDYIKQS GTDLGAKYPF QSTADIFDDI
510 520 530 540 550
VRWDLRFIID HWNSSGFDLW EEVNGMHFFT LLVQLSAVDR SLSYFNASER
560 570 580 590 600
SSPFVEELRQ TRRDISKFLV DPANGFINGK YNYIVETPMI ADTLRSGLDI
610 620 630 640 650
STLLAANTVH DAPSASHLPF DINDPAVLNT LHHLMLHMRS IYPINDSSKN
660 670 680 690 700
ATGIALGRYP EDVYDGYGVG EGNPWVLATC AASTTLYQLI YRHISEQHDL
710 720 730 740 750
VVPMNNDCSN AFWSELVFSN LTTLGNDEGY LILEFNTPAF NQTIQKIFQL
760
ADSFLVKLKA TWEQTGN
Length:767
Mass (Da):82,489
Last modified:November 1, 1990 - v2
Checksum:iA5F29E2427EDB593
GO

Sequence cautioni

The sequence CAA26487 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02649 Genomic DNA. Translation: CAA26487.1. Different initiation.
D00428 Genomic DNA. Translation: BAA00332.1.
PIRiA21896. ALBYG.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAMYH_YEASX
AccessioniPrimary (citable) accession number: P04065
Secondary accession number(s): Q92314
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1990
Last modified: July 5, 2017
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families