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Protein

Glucoamylase S1

Gene

STA1

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei455SubstrateBy similarity1
Active sitei518Proton acceptorPROSITE-ProRule annotation1
Active sitei521Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.3 5495

Protein family/group databases

CAZyiGH15 Glycoside Hydrolase Family 15

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase S1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAI
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:STA1
Synonyms:DEX2, MAL5
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000000147722 – 767Glucoamylase S1Add BLAST746

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi322N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi414N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi423N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi434N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi513N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi546N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi645N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi650N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi720N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi741N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP04065

Structurei

3D structure databases

ProteinModelPortaliP04065
SMRiP04065
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni348 – 691H subunitAdd BLAST344
Regioni692 – 767Y subunitAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 347Ser/Thr-richAdd BLAST326

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGG8 Eukaryota
COG3387 LUCA

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR011613 GH15-like
IPR000165 Glucoamylase
PfamiView protein in Pfam
PF00723 Glyco_hydro_15, 1 hit
PRINTSiPR00736 GLHYDRLASE15
SUPFAMiSSF48208 SSF48208, 1 hit
PROSITEiView protein in PROSITE
PS00820 GLUCOAMYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT
60 70 80 90 100
ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI
110 120 130 140 150
TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV
160 170 180 190 200
TTVVSTTVVT TEYSTSTKQG GEITTTFVTK NIPTTYLTTI APTSSVTTVT
210 220 230 240 250
NFTPTTITTT VCSTGTNSAG ETTSGCSPKT VTTTVPCSTG TGEYTTEATA
260 270 280 290 300
PVTTAVTTTV VTTESSTGTN SAGKTTTSYT TKSVPTTYVF DFGKGILDQS
310 320 330 340 350
CGGVFSNNGS SQVQLRDVVL MNGTVVYDSN GAWDSSALEE WLQRQKKVSI
360 370 380 390 400
ERIFENIGPS AVYPSILPGV VIASPSQTHP DYFYQWIRDS ALTINSIVSH
410 420 430 440 450
SADPAIETLL QYLNVSFHLQ RTNNTLGAGI GYTNDTVALG DPKWNVDNTA
460 470 480 490 500
FTEPWGRPQN DGPALRSIAI LKIIDYIKQS GTDLGAKYPF QSTADIFDDI
510 520 530 540 550
VRWDLRFIID HWNSSGFDLW EEVNGMHFFT LLVQLSAVDR SLSYFNASER
560 570 580 590 600
SSPFVEELRQ TRRDISKFLV DPANGFINGK YNYIVETPMI ADTLRSGLDI
610 620 630 640 650
STLLAANTVH DAPSASHLPF DINDPAVLNT LHHLMLHMRS IYPINDSSKN
660 670 680 690 700
ATGIALGRYP EDVYDGYGVG EGNPWVLATC AASTTLYQLI YRHISEQHDL
710 720 730 740 750
VVPMNNDCSN AFWSELVFSN LTTLGNDEGY LILEFNTPAF NQTIQKIFQL
760
ADSFLVKLKA TWEQTGN
Length:767
Mass (Da):82,489
Last modified:November 1, 1990 - v2
Checksum:iA5F29E2427EDB593
GO

Sequence cautioni

The sequence CAA26487 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02649 Genomic DNA Translation: CAA26487.1 Different initiation.
D00428 Genomic DNA Translation: BAA00332.1
PIRiA21896 ALBYG

Similar proteinsi

Entry informationi

Entry nameiAMYH_YEASX
AccessioniPrimary (citable) accession number: P04065
Secondary accession number(s): Q92314
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1990
Last modified: June 20, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

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