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P04065

- AMYH_YEASX

UniProt

P04065 - AMYH_YEASX

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Protein

Glucoamylase S1

Gene
STA1, DEX2, MAL5
Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei455 – 4551Substrate By similarity
Active sitei518 – 5181Proton acceptor By similarity
Active sitei521 – 5211Proton donor By similarity

GO - Molecular functioni

  1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.3. 8373.

Protein family/group databases

CAZyiGH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase S1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
GAI
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:STA1
Synonyms:DEX2, MAL5
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 767746Glucoamylase S1PRO_0000001477Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi308 – 3081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi322 – 3221N-linked (GlcNAc...) Reviewed prediction
Glycosylationi414 – 4141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi423 – 4231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi434 – 4341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi513 – 5131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi546 – 5461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi645 – 6451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi650 – 6501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi720 – 7201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi741 – 7411N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP04065.

Expressioni

Gene expression databases

GenevestigatoriP04065.

Structurei

3D structure databases

ProteinModelPortaliP04065.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni348 – 691344H subunitAdd
BLAST
Regioni692 – 76776Y subunitAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 347326Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3387.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view]
PfamiPF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PRINTSiPR00736. GLHYDRLASE15.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04065-1 [UniParc]FASTAAdd to Basket

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MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT    50
ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI 100
TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV 150
TTVVSTTVVT TEYSTSTKQG GEITTTFVTK NIPTTYLTTI APTSSVTTVT 200
NFTPTTITTT VCSTGTNSAG ETTSGCSPKT VTTTVPCSTG TGEYTTEATA 250
PVTTAVTTTV VTTESSTGTN SAGKTTTSYT TKSVPTTYVF DFGKGILDQS 300
CGGVFSNNGS SQVQLRDVVL MNGTVVYDSN GAWDSSALEE WLQRQKKVSI 350
ERIFENIGPS AVYPSILPGV VIASPSQTHP DYFYQWIRDS ALTINSIVSH 400
SADPAIETLL QYLNVSFHLQ RTNNTLGAGI GYTNDTVALG DPKWNVDNTA 450
FTEPWGRPQN DGPALRSIAI LKIIDYIKQS GTDLGAKYPF QSTADIFDDI 500
VRWDLRFIID HWNSSGFDLW EEVNGMHFFT LLVQLSAVDR SLSYFNASER 550
SSPFVEELRQ TRRDISKFLV DPANGFINGK YNYIVETPMI ADTLRSGLDI 600
STLLAANTVH DAPSASHLPF DINDPAVLNT LHHLMLHMRS IYPINDSSKN 650
ATGIALGRYP EDVYDGYGVG EGNPWVLATC AASTTLYQLI YRHISEQHDL 700
VVPMNNDCSN AFWSELVFSN LTTLGNDEGY LILEFNTPAF NQTIQKIFQL 750
ADSFLVKLKA TWEQTGN 767
Length:767
Mass (Da):82,489
Last modified:November 1, 1990 - v2
Checksum:iA5F29E2427EDB593
GO

Sequence cautioni

The sequence CAA26487.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02649 Genomic DNA. Translation: CAA26487.1. Different initiation.
D00428 Genomic DNA. Translation: BAA00332.1.
PIRiA21896. ALBYG.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02649 Genomic DNA. Translation: CAA26487.1 . Different initiation.
D00428 Genomic DNA. Translation: BAA00332.1 .
PIRi A21896. ALBYG.

3D structure databases

ProteinModelPortali P04065.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH15. Glycoside Hydrolase Family 15.

Proteomic databases

PRIDEi P04065.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG3387.

Enzyme and pathway databases

BRENDAi 3.2.1.3. 8373.

Gene expression databases

Genevestigatori P04065.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR000165. Glucoamylase.
IPR011613. Glyco_hydro_15.
[Graphical view ]
Pfami PF00723. Glyco_hydro_15. 1 hit.
[Graphical view ]
PRINTSi PR00736. GLHYDRLASE15.
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus."
    Yamashita I., Suzuki K., Fukui S.
    J. Bacteriol. 161:567-573(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Diastaticus / ATCC 60709 / 5106-9A.
  2. "Proteolytic processing of glucoamylase in the yeast Saccharomyces cerevisiae."
    Yamashita I., Suzuki K., Sakuzo F.
    Agric. Biol. Chem. 50:475-482(1986)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
  3. "Upstream regions of the yeast glucoamylase gene which are required for efficient transcription."
    Shima H., Inui M., Akada R., Yamashita I.
    Agric. Biol. Chem. 53:749-755(1989)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.

Entry informationi

Entry nameiAMYH_YEASX
AccessioniPrimary (citable) accession number: P04065
Secondary accession number(s): Q92314
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1990
Last modified: October 16, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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