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P04065

- AMYH_YEASX

UniProt

P04065 - AMYH_YEASX

Protein

Glucoamylase S1

Gene

STA1

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei455 – 4551SubstrateBy similarity
    Active sitei518 – 5181Proton acceptorPROSITE-ProRule annotation
    Active sitei521 – 5211Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Enzyme and pathway databases

    BRENDAi3.2.1.3. 8373.

    Protein family/group databases

    CAZyiGH15. Glycoside Hydrolase Family 15.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucoamylase S1 (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    GAI
    Glucan 1,4-alpha-glucosidase
    Gene namesi
    Name:STA1
    Synonyms:DEX2, MAL5
    OrganismiSaccharomyces cerevisiae (Baker's yeast)
    Taxonomic identifieri4932 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Add
    BLAST
    Chaini22 – 767746Glucoamylase S1PRO_0000001477Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi308 – 3081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi414 – 4141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi434 – 4341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi546 – 5461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi645 – 6451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi650 – 6501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi720 – 7201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi741 – 7411N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP04065.

    Expressioni

    Gene expression databases

    GenevestigatoriP04065.

    Structurei

    3D structure databases

    ProteinModelPortaliP04065.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni348 – 691344H subunitAdd
    BLAST
    Regioni692 – 76776Y subunitAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 347326Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 15 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3387.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view]
    PfamiPF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    PRINTSiPR00736. GLHYDRLASE15.
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04065-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQRPFLLAYL VLSLLFNSAL GFPTALVPRG SSSSNITSSG PSSTPFSSAT    50
    ESFSTGTTVT PSSSKYPGSK TETSVSSTTE TTIVPTTTTT SVITPSTTTI 100
    TTTVCSTGTN SAGETTSGCS PKTITTTVPC STSPSETASE STTTSPTTPV 150
    TTVVSTTVVT TEYSTSTKQG GEITTTFVTK NIPTTYLTTI APTSSVTTVT 200
    NFTPTTITTT VCSTGTNSAG ETTSGCSPKT VTTTVPCSTG TGEYTTEATA 250
    PVTTAVTTTV VTTESSTGTN SAGKTTTSYT TKSVPTTYVF DFGKGILDQS 300
    CGGVFSNNGS SQVQLRDVVL MNGTVVYDSN GAWDSSALEE WLQRQKKVSI 350
    ERIFENIGPS AVYPSILPGV VIASPSQTHP DYFYQWIRDS ALTINSIVSH 400
    SADPAIETLL QYLNVSFHLQ RTNNTLGAGI GYTNDTVALG DPKWNVDNTA 450
    FTEPWGRPQN DGPALRSIAI LKIIDYIKQS GTDLGAKYPF QSTADIFDDI 500
    VRWDLRFIID HWNSSGFDLW EEVNGMHFFT LLVQLSAVDR SLSYFNASER 550
    SSPFVEELRQ TRRDISKFLV DPANGFINGK YNYIVETPMI ADTLRSGLDI 600
    STLLAANTVH DAPSASHLPF DINDPAVLNT LHHLMLHMRS IYPINDSSKN 650
    ATGIALGRYP EDVYDGYGVG EGNPWVLATC AASTTLYQLI YRHISEQHDL 700
    VVPMNNDCSN AFWSELVFSN LTTLGNDEGY LILEFNTPAF NQTIQKIFQL 750
    ADSFLVKLKA TWEQTGN 767
    Length:767
    Mass (Da):82,489
    Last modified:November 1, 1990 - v2
    Checksum:iA5F29E2427EDB593
    GO

    Sequence cautioni

    The sequence CAA26487.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02649 Genomic DNA. Translation: CAA26487.1. Different initiation.
    D00428 Genomic DNA. Translation: BAA00332.1.
    PIRiA21896. ALBYG.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02649 Genomic DNA. Translation: CAA26487.1 . Different initiation.
    D00428 Genomic DNA. Translation: BAA00332.1 .
    PIRi A21896. ALBYG.

    3D structure databases

    ProteinModelPortali P04065.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH15. Glycoside Hydrolase Family 15.

    Proteomic databases

    PRIDEi P04065.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG3387.

    Enzyme and pathway databases

    BRENDAi 3.2.1.3. 8373.

    Gene expression databases

    Genevestigatori P04065.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR000165. Glucoamylase.
    IPR011613. Glyco_hydro_15.
    [Graphical view ]
    Pfami PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    PRINTSi PR00736. GLHYDRLASE15.
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00820. GLUCOAMYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus."
      Yamashita I., Suzuki K., Fukui S.
      J. Bacteriol. 161:567-573(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Diastaticus / ATCC 60709 / 5106-9A.
    2. "Proteolytic processing of glucoamylase in the yeast Saccharomyces cerevisiae."
      Yamashita I., Suzuki K., Sakuzo F.
      Agric. Biol. Chem. 50:475-482(1986)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
    3. "Upstream regions of the yeast glucoamylase gene which are required for efficient transcription."
      Shima H., Inui M., Akada R., Yamashita I.
      Agric. Biol. Chem. 53:749-755(1989)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64.

    Entry informationi

    Entry nameiAMYH_YEASX
    AccessioniPrimary (citable) accession number: P04065
    Secondary accession number(s): Q92314
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3