P04061 (RNAS1_CHESE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonuclease EC=3.1.27.5
Organism	Chelydra serpentina (Snapping turtle) (Testudo serpentina)
Taxonomic identifier	8475 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Testudines › Cryptodira › Testudinoidea › Chelydridae › Chelydra

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	Ribonuclease		PRO_0000057175
Regions
	Region	41 – 45	5	Substrate binding By similarity
Sites
	Active site	11	1	Proton acceptor By similarity
	Active site	114	1	Proton donor By similarity
	Binding site	6	1	Substrate By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	82	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	26 ↔ 81		By similarity
	Disulfide bond	40 ↔ 92		By similarity
	Disulfide bond	58 ↔ 107		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04061 [UniParc]. Last modified November 1, 1986. Version 1. Checksum: 77078988B4423123	FASTA	119	12,915
	10 20 30 40 50 60 ETRYEKFLRQ HVDYPKSSAP DSRTYCNQMM QRRGMTSPVC KFTNTFVHAS AASITTVCGS 70 80 90 100 110 GGTPASGDLR DSNASFALTT CRLQGGSQTP NCPYNADAST QRIRIACVGG LPVHYDKSI

References

[1]	"The amino acid sequence of snapping turtle (Chelydra serpentina) ribonuclease." Beintema J.J., Broos J., Meulenberg J., Schueller C. Eur. J. Biochem. 153:305-312(1985) [PubMed: 4076178] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Pancreas.
[2]	"Evolution of ribonuclease in relation to polypeptide folding mechanisms." Barnard E.A., Cohen M.S., Gold M.H., Kim J.K. Nature 240:395-398(1972) [PubMed: 4564316] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-30.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	NRST. A91155.
3D structure databases
ProteinModelPortal	P04061.
SMR	P04061. Positions 1-119.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_CHESE

Accession

Primary (citable) accession number: P04061

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	November 1, 1986
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program