P04055 (PA21B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase A2
EC=3.1.1.4

Alternative name(s):
Group IB phospholipase A2
Phosphatidylcholine 2-acylhydrolase 1B

Gene names

Name:

Pla2g1b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Monomer or homodimer. The inactive pro-form is a homotrimer By similarity.
Subcellular location	Secreted. Note: secreted from pancreatic acinar cells in its inactive form By similarity.
Post-translational modification	Activated by trypsin cleavage in the duodenum. Can also be activated by thrombin or autocatalytically By similarity.
Sequence similarities	Belongs to the phospholipase A2 family.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Autocatalytic cleavage Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to insulin stimulus Inferred from sequence or structural similarity PubMed 1918029. Source: BHF-UCL fatty acid biosynthetic process Inferred from direct assay PubMed 1918029. Source: BHF-UCL glucose transport Inferred from sequence or structural similarity PubMed 1918029. Source: BHF-UCL multicellular organismal lipid catabolic process Inferred from electronic annotation. Source: Compara phosphatidylcholine metabolic process Inferred from electronic annotation. Source: Compara phospholipid metabolic process Inferred from direct assay PubMed 9487151. Source: RGD positive regulation of DNA replication Inferred from direct assay PubMed 1918029. Source: BHF-UCL
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara secretory granule Inferred from direct assay PubMed 9487151. Source: RGD
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipase A2 activity Inferred from direct assay PubMed 9487151. Source: RGD cell surface binding Inferred from direct assay PubMed 1918029. Source: BHF-UCL receptor binding Inferred from direct assay PubMed 1918029. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 22	7	Activation peptide		PRO_0000022747
	Chain	23 – 146	124	Phospholipase A2		PRO_0000022748
Sites
	Active site	70	1
	Active site	121	1
	Metal binding	50	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	52	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	54	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	71	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	33 ↔ 99		By similarity
	Disulfide bond	49 ↔ 146		By similarity
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	66 ↔ 127		By similarity
	Disulfide bond	73 ↔ 120		By similarity
	Disulfide bond	83 ↔ 113		By similarity
	Disulfide bond	106 ↔ 118		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04055 [UniParc]. Last modified November 1, 1986. Version 1. Checksum: 7EC4F7A491B913D0	FASTA	146	16,424
	10 20 30 40 50 60 MKLLLLAALL TAGVTAHSIS TRAVWQFRNM IKCTIPGSDP LREYNNYGCY CGLGGSGTPV 70 80 90 100 110 120 DDLDRCCQTH DHCYNQAKKL ESCKFLIDNP YTNTYSYKCS GNVITCSDKN NDCESFICNC 130 140 DRQAAICFSK VPYNKEYKDL DTKKHC

References

[1]	"Dog and rat pancreatic phospholipases A2: complete amino acid sequences deduced from complementary DNAs." Ohara O., Tamaki M., Nakamura E., Tsuruta Y., Fujii Y., Shin M., Teraoka H., Okamoto M. J. Biochem. 99:733-739(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Presence of pancreatic-type phospholipase A2 mRNA in rat gastric mucosa and lung." Sakata T., Nakamura E., Tsuruta Y., Tamaki M., Teraoka H., Tojo H., Ono T., Okamoto M. Biochim. Biophys. Acta 1007:124-126(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Rat pancreatic phospholipase A2: purification, characterization, and N-terminal amino acid sequence." Ono T., Tojo H., Inoue K., Kagamiyama H., Yamano T., Okamoto M. J. Biochem. 96:785-792(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-54.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00036 mRNA. Translation: BAA00024.1.
IPI	IPI00192334.
PIR	PSRT. A92008.
RefSeq	NP_113773.1. NM_031585.1.
UniGene	Rn.4283.
3D structure databases
ProteinModelPortal	P04055.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000001525.
Proteomic databases
PaxDb	P04055.
PRIDE	P04055.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001525; ENSRNOP00000001525; ENSRNOG00000001153.
GeneID	29526.
KEGG	rno:29526.
UCSC	RGD:61949. rat.
Organism-specific databases
CTD	5319.
RGD	61949. Pla2g1b.
Phylogenomic databases
eggNOG	NOG290764.
GeneTree	ENSGT00690000101719.
HOGENOM	HOG000231749.
HOVERGEN	HBG008137.
InParanoid	P04055.
KO	K01047.
OMA	NKECEAF.
OrthoDB	EOG4GHZQJ.
Gene expression databases
Genevestigator	P04055.
GermOnline	ENSRNOG00000001153. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.90.10. 1 hit.
InterPro	IPR001211. PLipase_A2. IPR013090. PLipase_A2_AS. IPR016090. PLipase_A2_dom. [Graphical view]
PANTHER	PTHR11716. PTHR11716. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P04055.
ChEMBL	CHEMBL5016.
NextBio	609488.

Entry information

Entry name

PA21B_RAT

Accession

Primary (citable) accession number: P04055

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	November 1, 1986
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program