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Protein

DNA nucleotidylexotransferase

Gene

DNTT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).3 Publications

Cofactori

Mg2+1 PublicationNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi343MagnesiumBy similarity1
Metal bindingi345MagnesiumBy similarity1
Metal bindingi433MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA metabolic process Source: UniProtKB
  • DNA modification Source: UniProtKB-KW
  • response to ATP Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Terminal addition

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS02999-MONOMER.
SABIO-RKP04053.
SignaLinkiP04053.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.313 Publications)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name:
Terminal transferase1 Publication
Gene namesi
Name:DNTT
Synonyms:TDT1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:2983. DNTT.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • euchromatin Source: Ensembl
  • nuclear chromatin Source: Ensembl
  • nuclear matrix Source: Ensembl
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343D → E: Nearly abolishes enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi1791.
OpenTargetsiENSG00000107447.
PharmGKBiPA27449.

Chemistry databases

ChEMBLiCHEMBL4810.

Polymorphism and mutation databases

BioMutaiDNTT.
DMDMi311033533.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187911 – 509DNA nucleotidylexotransferaseAdd BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei134PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04053.
PaxDbiP04053.
PeptideAtlasiP04053.
PRIDEiP04053.

PTM databases

iPTMnetiP04053.
PhosphoSitePlusiP04053.

Expressioni

Gene expression databases

BgeeiENSG00000107447.
CleanExiHS_DNTT.
GenevisibleiP04053. HS.

Organism-specific databases

HPAiHPA044194.

Interactioni

Subunit structurei

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1 (PubMed:16371131).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RELQ048643EBI-1220259,EBI-307352

Protein-protein interaction databases

BioGridi108127. 9 interactors.
IntActiP04053. 3 interactors.
STRINGi9606.ENSP00000360216.

Chemistry databases

BindingDBiP04053.

Structurei

Secondary structure

1509
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 25Combined sources3
Beta strandi36 – 40Combined sources5
Turni42 – 44Combined sources3
Helixi46 – 58Combined sources13
Beta strandi73 – 78Combined sources6
Helixi81 – 90Combined sources10
Beta strandi100 – 103Combined sources4
Helixi104 – 112Combined sources9
Beta strandi120 – 124Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2COENMR-A19-125[»]
ProteinModelPortaliP04053.
SMRiP04053.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04053.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 124BRCTPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni151 – 509Mediates interaction with DNTTIP21 PublicationAdd BLAST359
Regioni258 – 262Involved in DNA bindingBy similarity5
Regioni333 – 338Deoxynucleoside triphosphate bindingBy similarity6
Regioni342 – 345Deoxynucleoside triphosphate bindingBy similarity4
Regioni448 – 449Deoxynucleoside triphosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiP04053.
KOiK00977.
OMAiKTWKAIR.
OrthoDBiEOG091G073W.
PhylomeDBiP04053.
TreeFamiTF103012.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04053-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPPRASHLS PRKKRPRQTG ALMASSPQDI KFQDLVVFIL EKKMGTTRRA
60 70 80 90 100
FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQA QKVQVSSQPE
110 120 130 140 150
LLDVSWLIEC IRAGKPVEMT GKHQLVVRRD YSDSTNPGPP KTPPIAVQKI
160 170 180 190 200
SQYACQRRTT LNNCNQIFTD AFDILAENCE FRENEDSCVT FMRAASVLKS
210 220 230 240 250
LPFTIISMKD TEGIPCLGSK VKGIIEEIIE DGESSEVKAV LNDERYQSFK
260 270 280 290 300
LFTSVFGVGL KTSEKWFRMG FRTLSKVRSD KSLKFTRMQK AGFLYYEDLV
310 320 330 340 350
SCVTRAEAEA VSVLVKEAVW AFLPDAFVTM TGGFRRGKKM GHDVDFLITS
360 370 380 390 400
PGSTEDEEQL LQKVMNLWEK KGLLLYYDLV ESTFEKLRLP SRKVDALDHF
410 420 430 440 450
QKCFLIFKLP RQRVDSDQSS WQEGKTWKAI RVDLVLCPYE RRAFALLGWT
460 470 480 490 500
GSRQFERDLR RYATHERKMI LDNHALYDKT KRIFLKAESE EEIFAHLGLD

YIEPWERNA
Length:509
Mass (Da):58,536
Last modified:November 2, 2010 - v3
Checksum:i0EC01E380FA7E5A2
GO
Isoform 2 (identifier: P04053-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     454-454: Missing.

Show »
Length:508
Mass (Da):58,408
Checksum:iC72DABE3ACAEADD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51F → L in BAD97037 (Ref. 4) Curated1
Sequence conflicti299L → P in BAD97037 (Ref. 4) Curated1
Sequence conflicti329T → A in BAD97037 (Ref. 4) Curated1
Sequence conflicti383T → A in BAD97037 (Ref. 4) Curated1
Sequence conflicti389L → M in BAD97037 (Ref. 4) Curated1
Sequence conflicti452S → SP in AAA53100 (PubMed:2833741).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_058200112R → G.5 PublicationsCorresponds to variant rs6584066dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038397454Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11722 mRNA. Translation: AAA36726.1.
M20703
, M22968, M20694, M20695, M20696, M20697, M20698, M20699, M20700, M20701, M20702 mRNA. Translation: AAA53100.1.
AB046378 mRNA. Translation: BAB72001.1.
AK223317 mRNA. Translation: BAD97037.1.
AL136181 Genomic DNA. Translation: CAH72984.1.
BC012920 mRNA. Translation: AAH12920.1.
M21195 Genomic DNA. Translation: AAA61137.1.
K01919 mRNA. Translation: AAA61136.1.
CCDSiCCDS44465.1. [P04053-2]
CCDS7447.1. [P04053-1]
PIRiA23924. WXHU.
RefSeqiNP_001017520.1. NM_001017520.1. [P04053-2]
NP_004079.3. NM_004088.3. [P04053-1]
UniGeneiHs.534206.

Genome annotation databases

EnsembliENST00000371174; ENSP00000360216; ENSG00000107447. [P04053-1]
ENST00000630152; ENSP00000486733; ENSG00000107447. [P04053-2]
GeneIDi1791.
KEGGihsa:1791.
UCSCiuc001kmf.4. human. [P04053-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11722 mRNA. Translation: AAA36726.1.
M20703
, M22968, M20694, M20695, M20696, M20697, M20698, M20699, M20700, M20701, M20702 mRNA. Translation: AAA53100.1.
AB046378 mRNA. Translation: BAB72001.1.
AK223317 mRNA. Translation: BAD97037.1.
AL136181 Genomic DNA. Translation: CAH72984.1.
BC012920 mRNA. Translation: AAH12920.1.
M21195 Genomic DNA. Translation: AAA61137.1.
K01919 mRNA. Translation: AAA61136.1.
CCDSiCCDS44465.1. [P04053-2]
CCDS7447.1. [P04053-1]
PIRiA23924. WXHU.
RefSeqiNP_001017520.1. NM_001017520.1. [P04053-2]
NP_004079.3. NM_004088.3. [P04053-1]
UniGeneiHs.534206.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2COENMR-A19-125[»]
ProteinModelPortaliP04053.
SMRiP04053.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108127. 9 interactors.
IntActiP04053. 3 interactors.
STRINGi9606.ENSP00000360216.

Chemistry databases

BindingDBiP04053.
ChEMBLiCHEMBL4810.

PTM databases

iPTMnetiP04053.
PhosphoSitePlusiP04053.

Polymorphism and mutation databases

BioMutaiDNTT.
DMDMi311033533.

Proteomic databases

MaxQBiP04053.
PaxDbiP04053.
PeptideAtlasiP04053.
PRIDEiP04053.

Protocols and materials databases

DNASUi1791.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371174; ENSP00000360216; ENSG00000107447. [P04053-1]
ENST00000630152; ENSP00000486733; ENSG00000107447. [P04053-2]
GeneIDi1791.
KEGGihsa:1791.
UCSCiuc001kmf.4. human. [P04053-1]

Organism-specific databases

CTDi1791.
DisGeNETi1791.
GeneCardsiDNTT.
H-InvDBHIX0009072.
HGNCiHGNC:2983. DNTT.
HPAiHPA044194.
MIMi187410. gene.
neXtProtiNX_P04053.
OpenTargetsiENSG00000107447.
PharmGKBiPA27449.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2534. Eukaryota.
COG1796. LUCA.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000263600.
HOVERGENiHBG003670.
InParanoidiP04053.
KOiK00977.
OMAiKTWKAIR.
OrthoDBiEOG091G073W.
PhylomeDBiP04053.
TreeFamiTF103012.

Enzyme and pathway databases

BioCyciZFISH:HS02999-MONOMER.
SABIO-RKP04053.
SignaLinkiP04053.

Miscellaneous databases

EvolutionaryTraceiP04053.
GeneWikiiTerminal_deoxynucleotidyl_transferase.
GenomeRNAii1791.
PROiP04053.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107447.
CleanExiHS_DNTT.
GenevisibleiP04053. HS.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
cd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTDT_HUMAN
AccessioniPrimary (citable) accession number: P04053
Secondary accession number(s): Q53FH1, Q5W103, Q96E50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 2, 2010
Last modified: November 30, 2016
This is version 182 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.