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Protein

DNA-directed RNA polymerase III subunit RPC1

Gene

RPO31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Zinc 1By similarity1
Metal bindingi70Zinc 1By similarity1
Metal bindingi77Zinc 1By similarity1
Metal bindingi80Zinc 1By similarity1
Metal bindingi107Zinc 2By similarity1
Metal bindingi110Zinc 2By similarity1
Metal bindingi154Zinc 2By similarity1
Metal bindingi511Magnesium; catalyticBy similarity1
Metal bindingi513Magnesium; catalyticBy similarity1
Metal bindingi515Magnesium; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33645-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC1 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C1
Alternative name(s):
DNA-directed RNA polymerase III largest subunit
RNA polymerase III subunit C160
Gene namesi
Name:RPO31
Synonyms:RPC1, RPC160
Ordered Locus Names:YOR116C
ORF Names:O3254, YOR3254C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR116C.
SGDiS000005642. RPO31.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase III complex Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi506T → I: Temperature-sensitive. 1
Mutagenesisi509N → Y: Temperature-sensitive. 1
Mutagenesisi518N → Q: Temperature-sensitive. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739531 – 1460DNA-directed RNA polymerase III subunit RPC1Add BLAST1460

Proteomic databases

MaxQBiP04051.
PRIDEiP04051.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Binary interactionsi

WithEntry#Exp.IntActNotes
RPC25P357185EBI-15810,EBI-15854

Protein-protein interaction databases

BioGridi34512. 130 interactors.
DIPiDIP-612N.
IntActiP04051. 40 interactors.
MINTiMINT-557787.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FJ8electron microscopy3.90A1-1460[»]
5FJ9electron microscopy4.60A1-1460[»]
5FJAelectron microscopy4.65A1-1460[»]
ProteinModelPortaliP04051.
SMRiP04051.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni858 – 870Bridging helixBy similarityAdd BLAST13

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

GeneTreeiENSGT00850000132392.
HOGENOMiHOG000222974.
InParanoidiP04051.
KOiK03018.
OMAiSAMNRMA.
OrthoDBiEOG092C01XQ.

Family and domain databases

InterProiIPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEVVVSETP KRIKGLEFSA LSAADIVAQS EVEVSTRDLF DLEKDRAPKA
60 70 80 90 100
NGALDPKMGV SSSSLECATC HGNLASCHGH FGHLKLALPV FHIGYFKATI
110 120 130 140 150
QILQGICKNC SAILLSETDK RQFLHELRRP GVDNLRRMGI LKKILDQCKK
160 170 180 190 200
QRRCLHCGAL NGVVKKAAAG AGSAALKIIH DTFRWVGKKS APEKDIWVGE
210 220 230 240 250
WKEVLAHNPE LERYVKRCMD DLNPLKTLNL FKQIKSADCE LLGIDATVPS
260 270 280 290 300
GRPETYIWRY LPAPPVCIRP SVMMQDSPAS NEDDLTVKLT EIVWTSSLIK
310 320 330 340 350
AGLDKGISIN NMMEHWDYLQ LTVAMYINSD SVNPAMLPGS SNGGGKVKPI
360 370 380 390 400
RGFCQRLKGK QGRFRGNLSG KRVDFSGRTV ISPDPNLSID EVAVPDRVAK
410 420 430 440 450
VLTYPEKVTR YNRHKLQELI VNGPNVHPGA NYLLKRNEDA RRNLRYGDRM
460 470 480 490 500
KLAKNLQIGD VVERHLEDGD VVLFNRQPSL HRLSILSHYA KIRPWRTFRL
510 520 530 540 550
NECVCTPYNA DFDGDEMNLH VPQTEEARAE AINLMGVKNN LLTPKSGEPI
560 570 580 590 600
IAATQDFITG SYLISHKDSF YDRATLTQLL SMMSDGIEHF DIPPPAIMKP
610 620 630 640 650
YYLWTGKQVF SLLIKPNHNS PVVINLDAKN KVFVPPKSKS LPNEMSQNDG
660 670 680 690 700
FVIIRGSQIL SGVMDKSVLG DGKKHSVFYT ILRDYGPQEA ANAMNRMAKL
710 720 730 740 750
CARFLGNRGF SIGINDVTPA DDLKQKKEEL VEIAYHKCDE LITLFNKGEL
760 770 780 790 800
ETQPGCNEEQ TLEAKIGGLL SKVREEVGDV CINELDNWNA PLIMATCGSK
810 820 830 840 850
GSTLNVSQMV AVVGQQIISG NRVPDGFQDR SLPHFPKNSK TPQSKGFVRN
860 870 880 890 900
SFFSGLSPPE FLFHAISGRE GLVDTAVKTA ETGYMSRRLM KSLEDLSCQY
910 920 930 940 950
DNTVRTSANG IVQFTYGGDG LDPLEMEGNA QPVNFNRSWD HAYNITFNNQ
960 970 980 990 1000
DKGLLPYAIM ETANEILGPL EERLVRYDNS GCLVKREDLN KAEYVDQYDA
1010 1020 1030 1040 1050
ERDFYHSLRE YINGKATALA NLRKSRGMLG LLEPPAKELQ GIDPDETVPD
1060 1070 1080 1090 1100
NVKTSVSQLY RISEKSVRKF LEIALFKYRK ARLEPGTAIG AIGAQSIGEP
1110 1120 1130 1140 1150
GTQMTLKTFH FAGVASMNVT LGVPRIKEII NASKVISTPI INAVLVNDND
1160 1170 1180 1190 1200
ERAARVVKGR VEKTLLSDVA FYVQDVYKDN LSFIQVRIDL GTIDKLQLEL
1210 1220 1230 1240 1250
TIEDIAVAIT RASKLKIQAS DVNIIGKDRI AINVFPEGYK AKSISTSAKE
1260 1270 1280 1290 1300
PSENDVFYRM QQLRRALPDV VVKGLPDISR AVINIRDDGK RELLVEGYGL
1310 1320 1330 1340 1350
RDVMCTDGVI GSRTTTNHVL EVFSVLGIEA ARYSIIREIN YTMSNHGMSV
1360 1370 1380 1390 1400
DPRHIQLLGD VMTYKGEVLG ITRFGLSKMR DSVLQLASFE KTTDHLFDAA
1410 1420 1430 1440 1450
FYMKKDAVEG VSECIILGQT MSIGTGSFKV VKGTNISEKD LVPKRCLFES
1460
LSNEAALKAN
Length:1,460
Mass (Da):162,301
Last modified:November 1, 1986 - v1
Checksum:iD1697EB2352BCA4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03129 Genomic DNA. Translation: CAA26905.1.
X90518 Genomic DNA. Translation: CAA62123.1.
X94335 Genomic DNA. Translation: CAA64036.1.
Z75024 Genomic DNA. Translation: CAA99314.1.
BK006948 Genomic DNA. Translation: DAA10891.1.
PIRiA00694. RNBY3L.
RefSeqiNP_014759.1. NM_001183535.1.

Genome annotation databases

EnsemblFungiiYOR116C; YOR116C; YOR116C.
GeneIDi854283.
KEGGisce:YOR116C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03129 Genomic DNA. Translation: CAA26905.1.
X90518 Genomic DNA. Translation: CAA62123.1.
X94335 Genomic DNA. Translation: CAA64036.1.
Z75024 Genomic DNA. Translation: CAA99314.1.
BK006948 Genomic DNA. Translation: DAA10891.1.
PIRiA00694. RNBY3L.
RefSeqiNP_014759.1. NM_001183535.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FJ8electron microscopy3.90A1-1460[»]
5FJ9electron microscopy4.60A1-1460[»]
5FJAelectron microscopy4.65A1-1460[»]
ProteinModelPortaliP04051.
SMRiP04051.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34512. 130 interactors.
DIPiDIP-612N.
IntActiP04051. 40 interactors.
MINTiMINT-557787.

Proteomic databases

MaxQBiP04051.
PRIDEiP04051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR116C; YOR116C; YOR116C.
GeneIDi854283.
KEGGisce:YOR116C.

Organism-specific databases

EuPathDBiFungiDB:YOR116C.
SGDiS000005642. RPO31.

Phylogenomic databases

GeneTreeiENSGT00850000132392.
HOGENOMiHOG000222974.
InParanoidiP04051.
KOiK03018.
OMAiSAMNRMA.
OrthoDBiEOG092C01XQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33645-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

PROiP04051.

Family and domain databases

InterProiIPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPC1_YEAST
AccessioniPrimary (citable) accession number: P04051
Secondary accession number(s): D6W2H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: November 2, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6020 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.