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Protein

DNA-directed RNA polymerase III subunit RPC1

Gene

RPO31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).By similarity

Miscellaneous

Present with 6020 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Zinc 1By similarity1
Metal bindingi70Zinc 1By similarity1
Metal bindingi77Zinc 1By similarity1
Metal bindingi80Zinc 1By similarity1
Metal bindingi107Zinc 2By similarity1
Metal bindingi110Zinc 2By similarity1
Metal bindingi154Zinc 2By similarity1
Metal bindingi511Magnesium; catalyticBy similarity1
Metal bindingi513Magnesium; catalyticBy similarity1
Metal bindingi515Magnesium; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33645-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-72086 mRNA Capping
R-SCE-72165 mRNA Splicing - Minor Pathway
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC1 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C1
Alternative name(s):
DNA-directed RNA polymerase III largest subunit
RNA polymerase III subunit C160
Gene namesi
Name:RPO31
Synonyms:RPC1, RPC160
Ordered Locus Names:YOR116C
ORF Names:O3254, YOR3254C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR116C
SGDiS000005642 RPO31

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi506T → I: Temperature-sensitive. 1
Mutagenesisi509N → Y: Temperature-sensitive. 1
Mutagenesisi518N → Q: Temperature-sensitive. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739531 – 1460DNA-directed RNA polymerase III subunit RPC1Add BLAST1460

Proteomic databases

MaxQBiP04051
PaxDbiP04051
PRIDEiP04051

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34512, 251 interactors
DIPiDIP-612N
IntActiP04051, 50 interactors
MINTiP04051
STRINGi4932.YOR116C

Structurei

Secondary structure

11460
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 19Combined sources9
Helixi23 – 29Combined sources7
Beta strandi31 – 33Combined sources3
Turni44 – 46Combined sources3
Beta strandi50 – 54Combined sources5
Beta strandi62 – 65Combined sources4
Beta strandi68 – 70Combined sources3
Beta strandi74 – 76Combined sources3
Beta strandi82 – 90Combined sources9
Helixi96 – 104Combined sources9
Turni108 – 111Combined sources4
Helixi118 – 127Combined sources10
Helixi134 – 149Combined sources16
Beta strandi155 – 157Combined sources3
Beta strandi163 – 166Combined sources4
Beta strandi178 – 182Combined sources5
Beta strandi183 – 185Combined sources3
Helixi192 – 207Combined sources16
Beta strandi211 – 214Combined sources4
Helixi224 – 232Combined sources9
Helixi238 – 241Combined sources4
Beta strandi248 – 250Combined sources3
Turni253 – 256Combined sources4
Beta strandi257 – 263Combined sources7
Turni266 – 268Combined sources3
Beta strandi276 – 279Combined sources4
Helixi284 – 304Combined sources21
Helixi311 – 326Combined sources16
Beta strandi346 – 349Combined sources4
Turni355 – 357Combined sources3
Turni359 – 361Combined sources3
Helixi363 – 366Combined sources4
Turni367 – 369Combined sources3
Beta strandi374 – 383Combined sources10
Beta strandi391 – 395Combined sources5
Turni396 – 401Combined sources6
Beta strandi404 – 407Combined sources4
Beta strandi409 – 412Combined sources4
Helixi413 – 422Combined sources10
Turni423 – 425Combined sources3
Beta strandi426 – 434Combined sources9
Beta strandi441 – 447Combined sources7
Beta strandi449 – 451Combined sources3
Helixi452 – 455Combined sources4
Beta strandi461 – 464Combined sources4
Beta strandi471 – 475Combined sources5
Helixi482 – 484Combined sources3
Beta strandi485 – 500Combined sources16
Beta strandi502 – 504Combined sources3
Turni505 – 509Combined sources5
Beta strandi512 – 514Combined sources3
Beta strandi516 – 520Combined sources5
Helixi527 – 533Combined sources7
Beta strandi534 – 537Combined sources4
Turni544 – 546Combined sources3
Helixi555 – 562Combined sources8
Helixi573 – 582Combined sources10
Turni583 – 585Combined sources3
Beta strandi599 – 601Combined sources3
Beta strandi603 – 605Combined sources3
Helixi607 – 614Combined sources8
Beta strandi617 – 619Combined sources3
Beta strandi625 – 628Combined sources4
Beta strandi638 – 642Combined sources5
Beta strandi647 – 649Combined sources3
Beta strandi651 – 657Combined sources7
Beta strandi659 – 661Combined sources3
Turni666 – 668Combined sources3
Beta strandi669 – 674Combined sources6
Helixi677 – 708Combined sources32
Turni713 – 717Combined sources5
Helixi723 – 745Combined sources23
Beta strandi754 – 756Combined sources3
Helixi760 – 782Combined sources23
Beta strandi783 – 785Combined sources3
Helixi790 – 796Combined sources7
Beta strandi797 – 800Combined sources4
Helixi803 – 809Combined sources7
Beta strandi818 – 821Combined sources4
Beta strandi826 – 829Combined sources4
Turni842 – 846Combined sources5
Turni852 – 854Combined sources3
Helixi858 – 876Combined sources19
Helixi878 – 881Combined sources4
Helixi884 – 889Combined sources6
Turni890 – 892Combined sources3
Beta strandi893 – 895Combined sources3
Beta strandi911 – 915Combined sources5
Helixi916 – 918Combined sources3
Beta strandi923 – 925Combined sources3
Turni928 – 930Combined sources3
Beta strandi931 – 933Combined sources3
Helixi935 – 945Combined sources11
Beta strandi949 – 951Combined sources3
Helixi958 – 962Combined sources5
Turni963 – 965Combined sources3
Beta strandi966 – 968Combined sources3
Turni969 – 973Combined sources5
Beta strandi979 – 983Combined sources5
Helixi986 – 988Combined sources3
Beta strandi989 – 991Combined sources3
Helixi1000 – 1025Combined sources26
Beta strandi1037 – 1040Combined sources4
Beta strandi1047 – 1049Combined sources3
Helixi1050 – 1057Combined sources8
Helixi1064 – 1079Combined sources16
Beta strandi1080 – 1082Combined sources3
Helixi1089 – 1098Combined sources10
Helixi1100 – 1103Combined sources4
Beta strandi1111 – 1115Combined sources5
Helixi1123 – 1130Combined sources8
Beta strandi1140 – 1146Combined sources7
Helixi1152 – 1159Combined sources8
Helixi1166 – 1169Combined sources4
Beta strandi1176 – 1178Combined sources3
Beta strandi1181 – 1183Combined sources3
Helixi1191 – 1195Combined sources5
Helixi1202 – 1210Combined sources9
Beta strandi1212 – 1217Combined sources6
Beta strandi1226 – 1228Combined sources3
Helixi1237 – 1243Combined sources7
Turni1244 – 1246Combined sources3
Beta strandi1248 – 1250Combined sources3
Helixi1251 – 1254Combined sources4
Helixi1256 – 1265Combined sources10
Beta strandi1266 – 1270Combined sources5
Beta strandi1272 – 1274Combined sources3
Beta strandi1281 – 1285Combined sources5
Beta strandi1287 – 1289Combined sources3
Beta strandi1291 – 1295Combined sources5
Turni1302 – 1304Combined sources3
Beta strandi1311 – 1313Combined sources3
Beta strandi1315 – 1317Combined sources3
Helixi1319 – 1325Combined sources7
Helixi1329 – 1344Combined sources16
Turni1345 – 1347Combined sources3
Helixi1353 – 1361Combined sources9
Turni1373 – 1375Combined sources3
Turni1377 – 1379Combined sources3
Helixi1383 – 1386Combined sources4
Beta strandi1388 – 1391Combined sources4
Helixi1395 – 1402Combined sources8
Turni1412 – 1418Combined sources7
Turni1424 – 1427Combined sources4
Beta strandi1428 – 1432Combined sources5
Turni1438 – 1440Combined sources3
Beta strandi1441 – 1443Combined sources3
Beta strandi1446 – 1449Combined sources4
Turni1450 – 1453Combined sources4
Helixi1454 – 1456Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FJ8electron microscopy3.90A1-1460[»]
5FJ9electron microscopy4.60A1-1460[»]
5FJAelectron microscopy4.65A1-1460[»]
6EU0electron microscopy4.00A1-1460[»]
6EU1electron microscopy3.40A1-1460[»]
6EU2electron microscopy3.40A1-1460[»]
6EU3electron microscopy3.30A1-1460[»]
6F40electron microscopy3.70A1-1460[»]
6F41electron microscopy4.30A1-1460[»]
6F42electron microscopy5.50A1-1460[»]
6F44electron microscopy4.20A1-1460[»]
ProteinModelPortaliP04051
SMRiP04051
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni858 – 870Bridging helixBy similarityAdd BLAST13

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.Curated

Phylogenomic databases

GeneTreeiENSGT00910000144114
HOGENOMiHOG000222974
InParanoidiP04051
KOiK03018
OMAiAVCPPYN
OrthoDBiEOG092C01XQ

Family and domain databases

CDDicd02736 RNAP_III_Rpc1_C, 1 hit
cd02583 RNAP_III_RPC1_N, 1 hit
Gene3Di1.10.132.30, 1 hit
InterProiView protein in InterPro
IPR000722 RNA_pol_asu
IPR006592 RNA_pol_N
IPR007080 RNA_pol_Rpb1_1
IPR007066 RNA_pol_Rpb1_3
IPR007083 RNA_pol_Rpb1_4
IPR007081 RNA_pol_Rpb1_5
IPR035698 RNAP_III_Rpc1_C
IPR035697 RNAP_III_RPC1_N
IPR038120 Rpb1_funnel_sf
PfamiView protein in Pfam
PF04997 RNA_pol_Rpb1_1, 1 hit
PF00623 RNA_pol_Rpb1_2, 1 hit
PF04983 RNA_pol_Rpb1_3, 1 hit
PF05000 RNA_pol_Rpb1_4, 1 hit
PF04998 RNA_pol_Rpb1_5, 1 hit
SMARTiView protein in SMART
SM00663 RPOLA_N, 1 hit

Sequencei

Sequence statusi: Complete.

P04051-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEVVVSETP KRIKGLEFSA LSAADIVAQS EVEVSTRDLF DLEKDRAPKA
60 70 80 90 100
NGALDPKMGV SSSSLECATC HGNLASCHGH FGHLKLALPV FHIGYFKATI
110 120 130 140 150
QILQGICKNC SAILLSETDK RQFLHELRRP GVDNLRRMGI LKKILDQCKK
160 170 180 190 200
QRRCLHCGAL NGVVKKAAAG AGSAALKIIH DTFRWVGKKS APEKDIWVGE
210 220 230 240 250
WKEVLAHNPE LERYVKRCMD DLNPLKTLNL FKQIKSADCE LLGIDATVPS
260 270 280 290 300
GRPETYIWRY LPAPPVCIRP SVMMQDSPAS NEDDLTVKLT EIVWTSSLIK
310 320 330 340 350
AGLDKGISIN NMMEHWDYLQ LTVAMYINSD SVNPAMLPGS SNGGGKVKPI
360 370 380 390 400
RGFCQRLKGK QGRFRGNLSG KRVDFSGRTV ISPDPNLSID EVAVPDRVAK
410 420 430 440 450
VLTYPEKVTR YNRHKLQELI VNGPNVHPGA NYLLKRNEDA RRNLRYGDRM
460 470 480 490 500
KLAKNLQIGD VVERHLEDGD VVLFNRQPSL HRLSILSHYA KIRPWRTFRL
510 520 530 540 550
NECVCTPYNA DFDGDEMNLH VPQTEEARAE AINLMGVKNN LLTPKSGEPI
560 570 580 590 600
IAATQDFITG SYLISHKDSF YDRATLTQLL SMMSDGIEHF DIPPPAIMKP
610 620 630 640 650
YYLWTGKQVF SLLIKPNHNS PVVINLDAKN KVFVPPKSKS LPNEMSQNDG
660 670 680 690 700
FVIIRGSQIL SGVMDKSVLG DGKKHSVFYT ILRDYGPQEA ANAMNRMAKL
710 720 730 740 750
CARFLGNRGF SIGINDVTPA DDLKQKKEEL VEIAYHKCDE LITLFNKGEL
760 770 780 790 800
ETQPGCNEEQ TLEAKIGGLL SKVREEVGDV CINELDNWNA PLIMATCGSK
810 820 830 840 850
GSTLNVSQMV AVVGQQIISG NRVPDGFQDR SLPHFPKNSK TPQSKGFVRN
860 870 880 890 900
SFFSGLSPPE FLFHAISGRE GLVDTAVKTA ETGYMSRRLM KSLEDLSCQY
910 920 930 940 950
DNTVRTSANG IVQFTYGGDG LDPLEMEGNA QPVNFNRSWD HAYNITFNNQ
960 970 980 990 1000
DKGLLPYAIM ETANEILGPL EERLVRYDNS GCLVKREDLN KAEYVDQYDA
1010 1020 1030 1040 1050
ERDFYHSLRE YINGKATALA NLRKSRGMLG LLEPPAKELQ GIDPDETVPD
1060 1070 1080 1090 1100
NVKTSVSQLY RISEKSVRKF LEIALFKYRK ARLEPGTAIG AIGAQSIGEP
1110 1120 1130 1140 1150
GTQMTLKTFH FAGVASMNVT LGVPRIKEII NASKVISTPI INAVLVNDND
1160 1170 1180 1190 1200
ERAARVVKGR VEKTLLSDVA FYVQDVYKDN LSFIQVRIDL GTIDKLQLEL
1210 1220 1230 1240 1250
TIEDIAVAIT RASKLKIQAS DVNIIGKDRI AINVFPEGYK AKSISTSAKE
1260 1270 1280 1290 1300
PSENDVFYRM QQLRRALPDV VVKGLPDISR AVINIRDDGK RELLVEGYGL
1310 1320 1330 1340 1350
RDVMCTDGVI GSRTTTNHVL EVFSVLGIEA ARYSIIREIN YTMSNHGMSV
1360 1370 1380 1390 1400
DPRHIQLLGD VMTYKGEVLG ITRFGLSKMR DSVLQLASFE KTTDHLFDAA
1410 1420 1430 1440 1450
FYMKKDAVEG VSECIILGQT MSIGTGSFKV VKGTNISEKD LVPKRCLFES
1460
LSNEAALKAN
Length:1,460
Mass (Da):162,301
Last modified:November 1, 1986 - v1
Checksum:iD1697EB2352BCA4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03129 Genomic DNA Translation: CAA26905.1
X90518 Genomic DNA Translation: CAA62123.1
X94335 Genomic DNA Translation: CAA64036.1
Z75024 Genomic DNA Translation: CAA99314.1
BK006948 Genomic DNA Translation: DAA10891.1
PIRiA00694 RNBY3L
RefSeqiNP_014759.1, NM_001183535.1

Genome annotation databases

EnsemblFungiiYOR116C; YOR116C; YOR116C
GeneIDi854283
KEGGisce:YOR116C

Similar proteinsi

Entry informationi

Entry nameiRPC1_YEAST
AccessioniPrimary (citable) accession number: P04051
Secondary accession number(s): D6W2H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: March 28, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health