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P04047

- KITH_CHICK

UniProt

P04047 - KITH_CHICK

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Protein

Thymidine kinase, cytosolic

Gene
TK1
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Proton acceptor Reviewed prediction
Binding sitei129 – 1291Substrate; via amide nitrogen By similarity
Metal bindingi154 – 1541Zinc By similarity
Metal bindingi157 – 1571Zinc By similarity
Binding sitei182 – 1821Substrate By similarity
Metal bindingi186 – 1861Zinc By similarity
Metal bindingi189 – 1891Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATP Inferred
Nucleotide bindingi58 – 603ATP By similarity
Nucleotide bindingi98 – 1014ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. identical protein binding Source: IntAct
  3. thymidine kinase activity Source: UniProtKB-EC
  4. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein homotetramerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21. 1306.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 18

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Thymidine kinase, cytosolicPRO_0000174946Add
BLAST

Proteomic databases

PaxDbiP04047.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8306403,EBI-8306403

Protein-protein interaction databases

STRINGi9031.ENSGALP00000011630.

Structurei

3D structure databases

ProteinModelPortaliP04047.
SMRiP04047. Positions 18-192.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 1775Substrate binding By similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.

Phylogenomic databases

eggNOGiCOG1435.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiP04047.
KOiK00857.
OMAiQILQCSP.
OrthoDBiEOG7MSMPX.
PhylomeDBiP04047.
TreeFamiTF314839.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04047-1 [UniParc]FASTAAdd to Basket

« Hide

MNCLTVPGVH PGSPGRPRGQ IQVIFGPMFS GKSTELMRRV RRFQLAQYRC    50
LLVKYAKDTR YCTTGVSTHD RNTMEARPAC ALQDVYQEAL GSAVIGIDEG 100
QFFPDIVEFC EKMANTGKTV IVAALDGTFQ RKAFGSILNL VPLAESVVKL 150
NAVCMECYRE ASYTKRLGAE REVEVIGGAD KYHSVCRACY FQKRPQQLGS 200
ENKENVPMGV KQLDMPASRK IFAS 224
Length:224
Mass (Da):24,872
Last modified:November 1, 1986 - v1
Checksum:iBF29BEED2892BCF6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02611 Genomic DNA. Translation: AAA49096.1.
PIRiA00608. KICHT.
RefSeqiNP_990229.1. NM_204898.1.
UniGeneiGga.421.

Genome annotation databases

EnsembliENSGALT00000011644; ENSGALP00000011630; ENSGALG00000007191.
GeneIDi395719.
KEGGigga:395719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02611 Genomic DNA. Translation: AAA49096.1 .
PIRi A00608. KICHT.
RefSeqi NP_990229.1. NM_204898.1.
UniGenei Gga.421.

3D structure databases

ProteinModelPortali P04047.
SMRi P04047. Positions 18-192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000011630.

Proteomic databases

PaxDbi P04047.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000011644 ; ENSGALP00000011630 ; ENSGALG00000007191 .
GeneIDi 395719.
KEGGi gga:395719.

Organism-specific databases

CTDi 7083.

Phylogenomic databases

eggNOGi COG1435.
GeneTreei ENSGT00390000011309.
HOGENOMi HOG000076390.
HOVERGENi HBG006215.
InParanoidi P04047.
KOi K00857.
OMAi QILQCSP.
OrthoDBi EOG7MSMPX.
PhylomeDBi P04047.
TreeFami TF314839.

Enzyme and pathway databases

BRENDAi 2.7.1.21. 1306.

Miscellaneous databases

NextBioi 20815789.
PROi P04047.

Family and domain databases

InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase."
    Kwoh T.J., Engler J.A.
    Nucleic Acids Res. 12:3959-3971(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genetic and physical analysis of the chicken tk gene."
    Merrill G.F., Harland R.M., Groudine M., McKnight S.L.
    Mol. Cell. Biol. 4:1769-1776(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKITH_CHICK
AccessioniPrimary (citable) accession number: P04047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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