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P04047

- KITH_CHICK

UniProt

P04047 - KITH_CHICK

Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Nov 1986)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei99 – 991Proton acceptorSequence Analysis
    Binding sitei129 – 1291Substrate; via amide nitrogenBy similarity
    Metal bindingi154 – 1541ZincBy similarity
    Metal bindingi157 – 1571ZincBy similarity
    Binding sitei182 – 1821SubstrateBy similarity
    Metal bindingi186 – 1861ZincBy similarity
    Metal bindingi189 – 1891ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338ATPCurated
    Nucleotide bindingi58 – 603ATPBy similarity
    Nucleotide bindingi98 – 1014ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. thymidine kinase activity Source: UniProtKB-EC
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein homotetramerization Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.1.21. 1306.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase, cytosolic (EC:2.7.1.21)
    Gene namesi
    Name:TK1
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome 18

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224Thymidine kinase, cytosolicPRO_0000174946Add
    BLAST

    Proteomic databases

    PaxDbiP04047.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-8306403,EBI-8306403

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000011630.

    Structurei

    3D structure databases

    ProteinModelPortaliP04047.
    SMRiP04047. Positions 18-192.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni173 – 1775Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1435.
    GeneTreeiENSGT00390000011309.
    HOGENOMiHOG000076390.
    HOVERGENiHBG006215.
    InParanoidiP04047.
    KOiK00857.
    OMAiQILQCSP.
    OrthoDBiEOG7MSMPX.
    PhylomeDBiP04047.
    TreeFamiTF314839.

    Family and domain databases

    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04047-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNCLTVPGVH PGSPGRPRGQ IQVIFGPMFS GKSTELMRRV RRFQLAQYRC    50
    LLVKYAKDTR YCTTGVSTHD RNTMEARPAC ALQDVYQEAL GSAVIGIDEG 100
    QFFPDIVEFC EKMANTGKTV IVAALDGTFQ RKAFGSILNL VPLAESVVKL 150
    NAVCMECYRE ASYTKRLGAE REVEVIGGAD KYHSVCRACY FQKRPQQLGS 200
    ENKENVPMGV KQLDMPASRK IFAS 224
    Length:224
    Mass (Da):24,872
    Last modified:November 1, 1986 - v1
    Checksum:iBF29BEED2892BCF6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02611 Genomic DNA. Translation: AAA49096.1.
    PIRiA00608. KICHT.
    RefSeqiNP_990229.1. NM_204898.1.
    UniGeneiGga.421.

    Genome annotation databases

    EnsembliENSGALT00000011644; ENSGALP00000011630; ENSGALG00000007191.
    GeneIDi395719.
    KEGGigga:395719.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02611 Genomic DNA. Translation: AAA49096.1 .
    PIRi A00608. KICHT.
    RefSeqi NP_990229.1. NM_204898.1.
    UniGenei Gga.421.

    3D structure databases

    ProteinModelPortali P04047.
    SMRi P04047. Positions 18-192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000011630.

    Proteomic databases

    PaxDbi P04047.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000011644 ; ENSGALP00000011630 ; ENSGALG00000007191 .
    GeneIDi 395719.
    KEGGi gga:395719.

    Organism-specific databases

    CTDi 7083.

    Phylogenomic databases

    eggNOGi COG1435.
    GeneTreei ENSGT00390000011309.
    HOGENOMi HOG000076390.
    HOVERGENi HBG006215.
    InParanoidi P04047.
    KOi K00857.
    OMAi QILQCSP.
    OrthoDBi EOG7MSMPX.
    PhylomeDBi P04047.
    TreeFami TF314839.

    Enzyme and pathway databases

    BRENDAi 2.7.1.21. 1306.

    Miscellaneous databases

    NextBioi 20815789.
    PROi P04047.

    Family and domain databases

    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase."
      Kwoh T.J., Engler J.A.
      Nucleic Acids Res. 12:3959-3971(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genetic and physical analysis of the chicken tk gene."
      Merrill G.F., Harland R.M., Groudine M., McKnight S.L.
      Mol. Cell. Biol. 4:1769-1776(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiKITH_CHICK
    AccessioniPrimary (citable) accession number: P04047
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1986
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3