P04047 (KITH_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase, cytosolic
EC=2.7.1.21

Gene names

Name:

TK1

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Subcellular location	Cytoplasm.
Miscellaneous	Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Thymidine kinase, cytosolic		PRO_0000174946
Regions
	Nucleotide binding	26 – 33	8	ATP Probable
	Nucleotide binding	58 – 60	3	ATP By similarity
	Nucleotide binding	98 – 101	4	ATP By similarity
	Region	173 – 177	5	Substrate binding By similarity
Sites
	Active site	99	1	Proton acceptor Potential
	Metal binding	154	1	Zinc By similarity
	Metal binding	157	1	Zinc By similarity
	Metal binding	186	1	Zinc By similarity
	Metal binding	189	1	Zinc By similarity
	Binding site	129	1	Substrate; via amide nitrogen By similarity
	Binding site	182	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04047 [UniParc]. Last modified November 1, 1986. Version 1. Checksum: BF29BEED2892BCF6	FASTA	224	24,872
	10 20 30 40 50 60 MNCLTVPGVH PGSPGRPRGQ IQVIFGPMFS GKSTELMRRV RRFQLAQYRC LLVKYAKDTR 70 80 90 100 110 120 YCTTGVSTHD RNTMEARPAC ALQDVYQEAL GSAVIGIDEG QFFPDIVEFC EKMANTGKTV 130 140 150 160 170 180 IVAALDGTFQ RKAFGSILNL VPLAESVVKL NAVCMECYRE ASYTKRLGAE REVEVIGGAD 190 200 210 220 KYHSVCRACY FQKRPQQLGS ENKENVPMGV KQLDMPASRK IFAS

References

[1]	"The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase." Kwoh T.J., Engler J.A. Nucleic Acids Res. 12:3959-3971(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic and physical analysis of the chicken tk gene." Merrill G.F., Harland R.M., Groudine M., McKnight S.L. Mol. Cell. Biol. 4:1769-1776(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02611 Genomic DNA. Translation: AAA49096.1.
IPI	IPI00585035.
PIR	KICHT. A00608.
RefSeq	NP_990229.1. NM_204898.1.
UniGene	Gga.421.
3D structure databases
ProteinModelPortal	P04047.
SMR	P04047. Positions 18-192.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000011630.
Proteomic databases
PaxDb	P04047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000011644; ENSGALP00000011630; ENSGALG00000007191.
GeneID	395719.
KEGG	gga:395719.
Organism-specific databases
CTD	7083.
Phylogenomic databases
eggNOG	COG1435.
GeneTree	ENSGT00390000011309.
HOGENOM	HOG000076390.
HOVERGEN	HBG006215.
InParanoid	P04047.
KO	K00857.
OMA	EFCEEMA.
OrthoDB	EOG4VHK7C.
Enzyme and pathway databases
BRENDA	2.7.1.21. 1306.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20815789.

Entry information

Entry name

KITH_CHICK

Accession

Primary (citable) accession number: P04047

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	November 1, 1986
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program