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P04047 (KITH_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase, cytosolic

EC=2.7.1.21
Gene names
Name:TK1
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subcellular location

Cytoplasm.

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Sequence similarities

Belongs to the thymidine kinase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-8306403,EBI-8306403

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224Thymidine kinase, cytosolic
PRO_0000174946

Regions

Nucleotide binding26 – 338ATP Probable
Nucleotide binding58 – 603ATP By similarity
Nucleotide binding98 – 1014ATP By similarity
Region173 – 1775Substrate binding By similarity

Sites

Active site991Proton acceptor Potential
Metal binding1541Zinc By similarity
Metal binding1571Zinc By similarity
Metal binding1861Zinc By similarity
Metal binding1891Zinc By similarity
Binding site1291Substrate; via amide nitrogen By similarity
Binding site1821Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P04047 [UniParc].

Last modified November 1, 1986. Version 1.
Checksum: BF29BEED2892BCF6

FASTA22424,872
        10         20         30         40         50         60 
MNCLTVPGVH PGSPGRPRGQ IQVIFGPMFS GKSTELMRRV RRFQLAQYRC LLVKYAKDTR 

        70         80         90        100        110        120 
YCTTGVSTHD RNTMEARPAC ALQDVYQEAL GSAVIGIDEG QFFPDIVEFC EKMANTGKTV 

       130        140        150        160        170        180 
IVAALDGTFQ RKAFGSILNL VPLAESVVKL NAVCMECYRE ASYTKRLGAE REVEVIGGAD 

       190        200        210        220 
KYHSVCRACY FQKRPQQLGS ENKENVPMGV KQLDMPASRK IFAS 

« Hide

References

[1]"The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase."
Kwoh T.J., Engler J.A.
Nucleic Acids Res. 12:3959-3971(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genetic and physical analysis of the chicken tk gene."
Merrill G.F., Harland R.M., Groudine M., McKnight S.L.
Mol. Cell. Biol. 4:1769-1776(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02611 Genomic DNA. Translation: AAA49096.1.
PIRKICHT. A00608.
RefSeqNP_990229.1. NM_204898.1.
UniGeneGga.421.

3D structure databases

ProteinModelPortalP04047.
SMRP04047. Positions 18-192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000011630.

Proteomic databases

PaxDbP04047.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000011644; ENSGALP00000011630; ENSGALG00000007191.
GeneID395719.
KEGGgga:395719.

Organism-specific databases

CTD7083.

Phylogenomic databases

eggNOGCOG1435.
GeneTreeENSGT00390000011309.
HOGENOMHOG000076390.
HOVERGENHBG006215.
InParanoidP04047.
KOK00857.
OMAVRRFQLA.
OrthoDBEOG7MSMPX.
PhylomeDBP04047.
TreeFamTF314839.

Enzyme and pathway databases

BRENDA2.7.1.21. 1306.

Family and domain databases

InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20815789.
PROP04047.

Entry information

Entry nameKITH_CHICK
AccessionPrimary (citable) accession number: P04047
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families