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Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Proton acceptorSequence Analysis
Binding sitei129 – 1291Substrate; via amide nitrogenBy similarity
Metal bindingi154 – 1541ZincBy similarity
Metal bindingi157 – 1571ZincBy similarity
Binding sitei182 – 1821SubstrateBy similarity
Metal bindingi186 – 1861ZincBy similarity
Metal bindingi189 – 1891ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATPCurated
Nucleotide bindingi58 – 603ATPBy similarity
Nucleotide bindingi98 – 1014ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • thymidine kinase activity Source: UniProtKB-EC
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21. 1306.
ReactomeiREACT_286667. Pyrimidine salvage reactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Thymidine kinase, cytosolicPRO_0000174946Add
BLAST

Proteomic databases

PaxDbiP04047.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8306403,EBI-8306403

Protein-protein interaction databases

STRINGi9031.ENSGALP00000011630.

Structurei

3D structure databases

ProteinModelPortaliP04047.
SMRiP04047. Positions 18-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 1775Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiCOG1435.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiP04047.
KOiK00857.
OMAiGMATHDK.
OrthoDBiEOG7MSMPX.
PhylomeDBiP04047.
TreeFamiTF314839.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCLTVPGVH PGSPGRPRGQ IQVIFGPMFS GKSTELMRRV RRFQLAQYRC
60 70 80 90 100
LLVKYAKDTR YCTTGVSTHD RNTMEARPAC ALQDVYQEAL GSAVIGIDEG
110 120 130 140 150
QFFPDIVEFC EKMANTGKTV IVAALDGTFQ RKAFGSILNL VPLAESVVKL
160 170 180 190 200
NAVCMECYRE ASYTKRLGAE REVEVIGGAD KYHSVCRACY FQKRPQQLGS
210 220
ENKENVPMGV KQLDMPASRK IFAS
Length:224
Mass (Da):24,872
Last modified:November 1, 1986 - v1
Checksum:iBF29BEED2892BCF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02611 Genomic DNA. Translation: AAA49096.1.
PIRiA00608. KICHT.
RefSeqiNP_990229.1. NM_204898.1.
UniGeneiGga.421.

Genome annotation databases

EnsembliENSGALT00000011644; ENSGALP00000011630; ENSGALG00000007191.
GeneIDi395719.
KEGGigga:395719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02611 Genomic DNA. Translation: AAA49096.1.
PIRiA00608. KICHT.
RefSeqiNP_990229.1. NM_204898.1.
UniGeneiGga.421.

3D structure databases

ProteinModelPortaliP04047.
SMRiP04047. Positions 18-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000011630.

Proteomic databases

PaxDbiP04047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000011644; ENSGALP00000011630; ENSGALG00000007191.
GeneIDi395719.
KEGGigga:395719.

Organism-specific databases

CTDi7083.

Phylogenomic databases

eggNOGiCOG1435.
GeneTreeiENSGT00390000011309.
HOGENOMiHOG000076390.
HOVERGENiHBG006215.
InParanoidiP04047.
KOiK00857.
OMAiGMATHDK.
OrthoDBiEOG7MSMPX.
PhylomeDBiP04047.
TreeFamiTF314839.

Enzyme and pathway databases

BRENDAi2.7.1.21. 1306.
ReactomeiREACT_286667. Pyrimidine salvage reactions.

Miscellaneous databases

NextBioi20815789.
PROiP04047.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase."
    Kwoh T.J., Engler J.A.
    Nucleic Acids Res. 12:3959-3971(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genetic and physical analysis of the chicken tk gene."
    Merrill G.F., Harland R.M., Groudine M., McKnight S.L.
    Mol. Cell. Biol. 4:1769-1776(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKITH_CHICK
AccessioniPrimary (citable) accession number: P04047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: April 1, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.