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Protein

Thymidine kinase, cytosolic

Gene

TK1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99Proton acceptorSequence analysis1
Binding sitei129Substrate; via amide nitrogenBy similarity1
Metal bindingi154ZincBy similarity1
Metal bindingi157ZincBy similarity1
Binding sitei182SubstrateBy similarity1
Metal bindingi186ZincBy similarity1
Metal bindingi189ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33ATPCurated8
Nucleotide bindingi58 – 60ATPBy similarity3
Nucleotide bindingi98 – 101ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • thymidine kinase activity Source: GO_Central
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processDNA synthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.21 1306
ReactomeiR-GGA-73614 Pyrimidine salvage

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase, cytosolic (EC:2.7.1.21)
Gene namesi
Name:TK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 18

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001749461 – 224Thymidine kinase, cytosolicAdd BLAST224

Proteomic databases

PaxDbiP04047
PRIDEiP04047

Expressioni

Gene expression databases

BgeeiENSGALG00000007191

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8306403,EBI-8306403

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

MINTiP04047
STRINGi9031.ENSGALP00000011630

Structurei

3D structure databases

ProteinModelPortaliP04047
SMRiP04047
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 177Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiKOG3125 Eukaryota
COG1435 LUCA
GeneTreeiENSGT00390000011309
HOGENOMiHOG000076390
HOVERGENiHBG006215
InParanoidiP04047
KOiK00857
OMAiCAGLDQD
OrthoDBiEOG091G0HZR
PhylomeDBiP04047
TreeFamiTF314839

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR001267 Thymidine_kinase
IPR020633 Thymidine_kinase_CS
PANTHERiPTHR11441 PTHR11441, 1 hit
PfamiView protein in Pfam
PF00265 TK, 1 hit
PIRSFiPIRSF035805 TK_cell, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00603 TK_CELLULAR_TYPE, 1 hit

Sequencei

Sequence statusi: Complete.

P04047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCLTVPGVH PGSPGRPRGQ IQVIFGPMFS GKSTELMRRV RRFQLAQYRC
60 70 80 90 100
LLVKYAKDTR YCTTGVSTHD RNTMEARPAC ALQDVYQEAL GSAVIGIDEG
110 120 130 140 150
QFFPDIVEFC EKMANTGKTV IVAALDGTFQ RKAFGSILNL VPLAESVVKL
160 170 180 190 200
NAVCMECYRE ASYTKRLGAE REVEVIGGAD KYHSVCRACY FQKRPQQLGS
210 220
ENKENVPMGV KQLDMPASRK IFAS
Length:224
Mass (Da):24,872
Last modified:November 1, 1986 - v1
Checksum:iBF29BEED2892BCF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02611 Genomic DNA Translation: AAA49096.1
PIRiA00608 KICHT
RefSeqiNP_990229.1, NM_204898.1
UniGeneiGga.421

Genome annotation databases

EnsembliENSGALT00000082171; ENSGALP00000053115; ENSGALG00000007191
GeneIDi395719
KEGGigga:395719

Similar proteinsi

Entry informationi

Entry nameiKITH_CHICK
AccessioniPrimary (citable) accession number: P04047
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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