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P04046

- PUR1_YEAST

UniProt

P04046 - PUR1_YEAST

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Protein
Amidophosphoribosyltransferase
Gene
ADE4, YMR300C, YM9952.02C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Metal bindingi373 – 3731Magnesium By similarity
Metal bindingi374 – 3741Magnesium By similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: SGD
  2. identical protein binding Source: IntAct
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ADE4
Ordered Locus Names:YMR300C
ORF Names:YM9952.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR300c.
SGDiS000004915. ADE4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 510509Amidophosphoribosyltransferase
PRO_0000139647Add
BLAST

Proteomic databases

MaxQBiP04046.
PaxDbiP04046.
PeptideAtlasiP04046.

Expressioni

Gene expression databases

GenevestigatoriP04046.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-14238,EBI-14238

Protein-protein interaction databases

BioGridi35480. 33 interactions.
DIPiDIP-6727N.
IntActiP04046. 9 interactions.
MINTiMINT-625374.
STRINGi4932.YMR300C.

Structurei

3D structure databases

ProteinModelPortaliP04046.
SMRiP04046. Positions 2-490.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 239238Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
GeneTreeiENSGT00390000003428.
HOGENOMiHOG000033687.
KOiK00764.
OMAiPITHAHI.
OrthoDBiEOG74R20H.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04046-1 [UniParc]FASTAAdd to Basket

« Hide

MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK    50
GNGMARDVFT QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI 100
NLAHNGNLVN TASLKRYMDE DVHRHINTDS DSELLLNIFA AELEKHNKYR 150
VNNEDVFHAL EGVYRLCRGG YACVGLLAGF ALFGFRDPNG IRPLLFGERE 200
NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC SKGEPEFKQV 250
VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE 300
DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV 350
SSVRRKLNPM ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA 400
SAAPAIRYNH IYGIDLTDTK NLIAYNRTDE EVAEVIGCER VIYQSLEDLI 450
DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ ELEEKRESIA NNSSDMKAEV 500
DIGLYNCADY 510
Length:510
Mass (Da):56,719
Last modified:February 1, 1996 - v2
Checksum:i1FE7A6F5B7C96710
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 472IY → VC in AAA34403. 1 Publication
Sequence conflicti46 – 472IY → VC in AAA34404. 1 Publication
Sequence conflicti62 – 621Missing in AAA34403. 1 Publication
Sequence conflicti62 – 621Missing in AAA34404. 1 Publication
Sequence conflicti82 – 876GSSANS → PLRLIL1 Publication
Sequence conflicti156 – 1583VFH → GFSN in AAA34403. 1 Publication
Sequence conflicti156 – 1583VFH → GFSN in AAA34404. 1 Publication
Sequence conflicti234 – 2341V → G in AAA34403. 1 Publication
Sequence conflicti234 – 2341V → G in AAA34404. 1 Publication
Sequence conflicti291 – 2911E → Q in AAA34403. 1 Publication
Sequence conflicti291 – 2911E → Q in AAA34404. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02203 Genomic DNA. Translation: AAA34403.1.
M57633 Genomic DNA. Translation: AAA34405.1.
M74309 Genomic DNA. Translation: AAA34404.1.
Z49212 Genomic DNA. Translation: CAA89133.1.
BK006946 Genomic DNA. Translation: DAA10201.1.
PIRiS53970.
RefSeqiNP_014029.1. NM_001182809.1.

Genome annotation databases

EnsemblFungiiYMR300C; YMR300C; YMR300C.
GeneIDi855346.
KEGGisce:YMR300C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02203 Genomic DNA. Translation: AAA34403.1 .
M57633 Genomic DNA. Translation: AAA34405.1 .
M74309 Genomic DNA. Translation: AAA34404.1 .
Z49212 Genomic DNA. Translation: CAA89133.1 .
BK006946 Genomic DNA. Translation: DAA10201.1 .
PIRi S53970.
RefSeqi NP_014029.1. NM_001182809.1.

3D structure databases

ProteinModelPortali P04046.
SMRi P04046. Positions 2-490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35480. 33 interactions.
DIPi DIP-6727N.
IntActi P04046. 9 interactions.
MINTi MINT-625374.
STRINGi 4932.YMR300C.

Protein family/group databases

MEROPSi C44.001.

Proteomic databases

MaxQBi P04046.
PaxDbi P04046.
PeptideAtlasi P04046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR300C ; YMR300C ; YMR300C .
GeneIDi 855346.
KEGGi sce:YMR300C.

Organism-specific databases

CYGDi YMR300c.
SGDi S000004915. ADE4.

Phylogenomic databases

eggNOGi COG0034.
GeneTreei ENSGT00390000003428.
HOGENOMi HOG000033687.
KOi K00764.
OMAi PITHAHI.
OrthoDBi EOG74R20H.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci MetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.

Miscellaneous databases

NextBioi 979094.
PROi P04046.

Gene expression databases

Genevestigatori P04046.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase."
    Maentsaelae P., Zalkin H.
    J. Biol. Chem. 259:8478-8484(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Deletion analysis of the 5' flanking region of yeast ADE4 gene."
    Lahti R., Glumoff V., Haemaelaeinen H., Paerssinen R., Maentsaelae P.
    Eur. Congr. Biotechnol. 1:420-423(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUR1_YEAST
AccessioniPrimary (citable) accession number: P04046
Secondary accession number(s): D6W0C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18700 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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