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P04046

- PUR1_YEAST

UniProt

P04046 - PUR1_YEAST

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Protein

Amidophosphoribosyltransferase

Gene

ADE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21NucleophilePROSITE-ProRule annotation
Metal bindingi373 – 3731MagnesiumBy similarity
Metal bindingi374 – 3741MagnesiumBy similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: SGD
  2. identical protein binding Source: IntAct
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ADE4
Ordered Locus Names:YMR300C
ORF Names:YM9952.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR300c.
SGDiS000004915. ADE4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 510509AmidophosphoribosyltransferasePRO_0000139647Add
BLAST

Proteomic databases

MaxQBiP04046.
PaxDbiP04046.
PeptideAtlasiP04046.

Expressioni

Gene expression databases

GenevestigatoriP04046.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-14238,EBI-14238

Protein-protein interaction databases

BioGridi35480. 35 interactions.
DIPiDIP-6727N.
IntActiP04046. 9 interactions.
MINTiMINT-625374.
STRINGi4932.YMR300C.

Structurei

3D structure databases

ProteinModelPortaliP04046.
SMRiP04046. Positions 2-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 239238Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
GeneTreeiENSGT00390000003428.
HOGENOMiHOG000033687.
InParanoidiP04046.
KOiK00764.
OMAiPITHAHI.
OrthoDBiEOG74R20H.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04046-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK
60 70 80 90 100
GNGMARDVFT QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI
110 120 130 140 150
NLAHNGNLVN TASLKRYMDE DVHRHINTDS DSELLLNIFA AELEKHNKYR
160 170 180 190 200
VNNEDVFHAL EGVYRLCRGG YACVGLLAGF ALFGFRDPNG IRPLLFGERE
210 220 230 240 250
NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC SKGEPEFKQV
260 270 280 290 300
VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE
310 320 330 340 350
DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV
360 370 380 390 400
SSVRRKLNPM ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA
410 420 430 440 450
SAAPAIRYNH IYGIDLTDTK NLIAYNRTDE EVAEVIGCER VIYQSLEDLI
460 470 480 490 500
DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ ELEEKRESIA NNSSDMKAEV
510
DIGLYNCADY
Length:510
Mass (Da):56,719
Last modified:February 1, 1996 - v2
Checksum:i1FE7A6F5B7C96710
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 472IY → VC in AAA34403. (PubMed:6376509)Curated
Sequence conflicti46 – 472IY → VC in AAA34404. (PubMed:6376509)Curated
Sequence conflicti62 – 621Missing in AAA34403. (PubMed:6376509)Curated
Sequence conflicti62 – 621Missing in AAA34404. (PubMed:6376509)Curated
Sequence conflicti82 – 876GSSANS → PLRLIL(PubMed:6376509)Curated
Sequence conflicti156 – 1583VFH → GFSN in AAA34403. (PubMed:6376509)Curated
Sequence conflicti156 – 1583VFH → GFSN in AAA34404. (PubMed:6376509)Curated
Sequence conflicti234 – 2341V → G in AAA34403. (PubMed:6376509)Curated
Sequence conflicti234 – 2341V → G in AAA34404. (PubMed:6376509)Curated
Sequence conflicti291 – 2911E → Q in AAA34403. (PubMed:6376509)Curated
Sequence conflicti291 – 2911E → Q in AAA34404. (PubMed:6376509)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02203 Genomic DNA. Translation: AAA34403.1.
M57633 Genomic DNA. Translation: AAA34405.1.
M74309 Genomic DNA. Translation: AAA34404.1.
Z49212 Genomic DNA. Translation: CAA89133.1.
BK006946 Genomic DNA. Translation: DAA10201.1.
PIRiS53970.
RefSeqiNP_014029.1. NM_001182809.1.

Genome annotation databases

EnsemblFungiiYMR300C; YMR300C; YMR300C.
GeneIDi855346.
KEGGisce:YMR300C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02203 Genomic DNA. Translation: AAA34403.1 .
M57633 Genomic DNA. Translation: AAA34405.1 .
M74309 Genomic DNA. Translation: AAA34404.1 .
Z49212 Genomic DNA. Translation: CAA89133.1 .
BK006946 Genomic DNA. Translation: DAA10201.1 .
PIRi S53970.
RefSeqi NP_014029.1. NM_001182809.1.

3D structure databases

ProteinModelPortali P04046.
SMRi P04046. Positions 2-490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35480. 35 interactions.
DIPi DIP-6727N.
IntActi P04046. 9 interactions.
MINTi MINT-625374.
STRINGi 4932.YMR300C.

Protein family/group databases

MEROPSi C44.001.

Proteomic databases

MaxQBi P04046.
PaxDbi P04046.
PeptideAtlasi P04046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR300C ; YMR300C ; YMR300C .
GeneIDi 855346.
KEGGi sce:YMR300C.

Organism-specific databases

CYGDi YMR300c.
SGDi S000004915. ADE4.

Phylogenomic databases

eggNOGi COG0034.
GeneTreei ENSGT00390000003428.
HOGENOMi HOG000033687.
InParanoidi P04046.
KOi K00764.
OMAi PITHAHI.
OrthoDBi EOG74R20H.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci MetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.

Miscellaneous databases

NextBioi 979094.
PROi P04046.

Gene expression databases

Genevestigatori P04046.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPi MF_01931. PurF.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase."
    Maentsaelae P., Zalkin H.
    J. Biol. Chem. 259:8478-8484(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Deletion analysis of the 5' flanking region of yeast ADE4 gene."
    Lahti R., Glumoff V., Haemaelaeinen H., Paerssinen R., Maentsaelae P.
    Eur. Congr. Biotechnol. 1:420-423(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUR1_YEAST
AccessioniPrimary (citable) accession number: P04046
Secondary accession number(s): D6W0C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3