P04046 (PUR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase

Gene names

Name:	ADE4
Ordered Locus Names:	YMR300C
ORF Names:	YM9952.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Miscellaneous	Present with 18700 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from mutant phenotype. Source: SGD glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	amidophosphoribosyltransferase activity Inferred from mutant phenotype Ref.1. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Amidophosphoribosyltransferase		PRO_0000139647
Regions
	Domain	2 – 239	238	Glutamine amidotransferase type-2
Sites
	Active site	2	1	For GATase activity By similarity
	Metal binding	373	1	Magnesium By similarity
	Metal binding	374	1	Magnesium By similarity
Amino acid modifications
	Modified residue	488	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	46 – 47	2	IY → VC in AAA34403. Ref.1
	Sequence conflict	46 – 47	2	IY → VC in AAA34404. Ref.1
	Sequence conflict	62	1	Missing in AAA34403. Ref.1
	Sequence conflict	62	1	Missing in AAA34404. Ref.1
	Sequence conflict	82 – 87	6	GSSANS → PLRLIL Ref.1
	Sequence conflict	156 – 158	3	VFH → GFSN in AAA34403. Ref.1
	Sequence conflict	156 – 158	3	VFH → GFSN in AAA34404. Ref.1
	Sequence conflict	234	1	V → G in AAA34403. Ref.1
	Sequence conflict	234	1	V → G in AAA34404. Ref.1
	Sequence conflict	291	1	E → Q in AAA34403. Ref.1
	Sequence conflict	291	1	E → Q in AAA34404. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P04046 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 1FE7A6F5B7C96710	FASTA	510	56,719
	10 20 30 40 50 60 MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK GNGMARDVFT 70 80 90 100 110 120 QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI NLAHNGNLVN TASLKRYMDE 130 140 150 160 170 180 DVHRHINTDS DSELLLNIFA AELEKHNKYR VNNEDVFHAL EGVYRLCRGG YACVGLLAGF 190 200 210 220 230 240 ALFGFRDPNG IRPLLFGERE NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC 250 260 270 280 290 300 SKGEPEFKQV VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE 310 320 330 340 350 360 DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV SSVRRKLNPM 370 380 390 400 410 420 ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA SAAPAIRYNH IYGIDLTDTK 430 440 450 460 470 480 NLIAYNRTDE EVAEVIGCER VIYQSLEDLI DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ 490 500 510 ELEEKRESIA NNSSDMKAEV DIGLYNCADY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase." Maentsaelae P., Zalkin H. J. Biol. Chem. 259:8478-8484(1984) [PubMed: 6376509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Deletion analysis of the 5' flanking region of yeast ADE4 gene." Lahti R., Glumoff V., Haemaelaeinen H., Paerssinen R., Maentsaelae P. Eur. Congr. Biotechnol. 1:420-423(1987) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02203 Genomic DNA. Translation: AAA34403.1. M57633 Genomic DNA. Translation: AAA34405.1. M74309 Genomic DNA. Translation: AAA34404.1. Z49212 Genomic DNA. Translation: CAA89133.1. BK006946 Genomic DNA. Translation: DAA10201.1.
PIR	S53970.
RefSeq	NP_014029.1. NM_001182809.1.
3D structure databases
ProteinModelPortal	P04046.
SMR	P04046. Positions 2-491.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6727N.
IntAct	P04046. 17 interactions.
MINT	MINT-625374.
STRING	P04046.
Proteomic databases
PeptideAtlas	P04046.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR300C; YMR300C; YMR300C.
GeneID	855346.
KEGG	sce:YMR300C.
NMPDR	fig|4932.3.peg.5087.
Organism-specific databases
CYGD	YMR300c.
SGD	S000004915. ADE4.
Phylogenomic databases
eggNOG	fuNOG05803.
GeneTree	EFGT00050000003544.
HOGENOM	HBG392416.
OMA	YFASCAP.
OrthoDB	EOG4B5SDH.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-462.
Gene expression databases
ArrayExpress	P04046.
Genevestigator	P04046.
GermOnline	YMR300C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
KO	K00764.
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979094.

Entry information

Entry name

PUR1_YEAST

Accession

Primary (citable) accession number: P04046
Secondary accession number(s): D6W0C7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program