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P04046 (PUR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 151. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene names
Name:ADE4
Ordered Locus Names:YMR300C
ORF Names:YM9952.02C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Miscellaneous

Present with 18700 molecules/cell in log phase SD medium.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself3EBI-14238,EBI-14238

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 510509Amidophosphoribosyltransferase
PRO_0000139647

Regions

Domain2 – 239238Glutamine amidotransferase type-2

Sites

Active site21For GATase activity By similarity
Metal binding3731Magnesium By similarity
Metal binding3741Magnesium By similarity

Experimental info

Sequence conflict46 – 472IY → VC in AAA34403. Ref.1
Sequence conflict46 – 472IY → VC in AAA34404. Ref.1
Sequence conflict621Missing in AAA34403. Ref.1
Sequence conflict621Missing in AAA34404. Ref.1
Sequence conflict82 – 876GSSANS → PLRLIL Ref.1
Sequence conflict156 – 1583VFH → GFSN in AAA34403. Ref.1
Sequence conflict156 – 1583VFH → GFSN in AAA34404. Ref.1
Sequence conflict2341V → G in AAA34403. Ref.1
Sequence conflict2341V → G in AAA34404. Ref.1
Sequence conflict2911E → Q in AAA34403. Ref.1
Sequence conflict2911E → Q in AAA34404. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P04046 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 1FE7A6F5B7C96710

FASTA51056,719
        10         20         30         40         50         60 
MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK GNGMARDVFT 

        70         80         90        100        110        120 
QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI NLAHNGNLVN TASLKRYMDE 

       130        140        150        160        170        180 
DVHRHINTDS DSELLLNIFA AELEKHNKYR VNNEDVFHAL EGVYRLCRGG YACVGLLAGF 

       190        200        210        220        230        240 
ALFGFRDPNG IRPLLFGERE NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC 

       250        260        270        280        290        300 
SKGEPEFKQV VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE 

       310        320        330        340        350        360 
DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV SSVRRKLNPM 

       370        380        390        400        410        420 
ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA SAAPAIRYNH IYGIDLTDTK 

       430        440        450        460        470        480 
NLIAYNRTDE EVAEVIGCER VIYQSLEDLI DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ 

       490        500        510 
ELEEKRESIA NNSSDMKAEV DIGLYNCADY 

« Hide

References

« Hide 'large scale' references
[1]"Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase."
Maentsaelae P., Zalkin H.
J. Biol. Chem. 259:8478-8484(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Deletion analysis of the 5' flanking region of yeast ADE4 gene."
Lahti R., Glumoff V., Haemaelaeinen H., Paerssinen R., Maentsaelae P.
Eur. Congr. Biotechnol. 1:420-423(1987)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02203 Genomic DNA. Translation: AAA34403.1.
M57633 Genomic DNA. Translation: AAA34405.1.
M74309 Genomic DNA. Translation: AAA34404.1.
Z49212 Genomic DNA. Translation: CAA89133.1.
BK006946 Genomic DNA. Translation: DAA10201.1.
PIRS53970.
RefSeqNP_014029.1. NM_001182809.1.

3D structure databases

ProteinModelPortalP04046.
SMRP04046. Positions 2-490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35480. 33 interactions.
DIPDIP-6727N.
IntActP04046. 9 interactions.
MINTMINT-625374.
STRING4932.YMR300C.

Protein family/group databases

MEROPSC44.001.

Proteomic databases

MaxQBP04046.
PaxDbP04046.
PeptideAtlasP04046.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR300C; YMR300C; YMR300C.
GeneID855346.
KEGGsce:YMR300C.

Organism-specific databases

CYGDYMR300c.
SGDS000004915. ADE4.

Phylogenomic databases

eggNOGCOG0034.
GeneTreeENSGT00390000003428.
HOGENOMHOG000033687.
KOK00764.
OMAPITHAHI.
OrthoDBEOG74R20H.

Enzyme and pathway databases

BioCycMetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.
UniPathwayUPA00074; UER00124.

Gene expression databases

GenevestigatorP04046.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979094.
PROP04046.

Entry information

Entry namePUR1_YEAST
AccessionPrimary (citable) accession number: P04046
Secondary accession number(s): D6W0C7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways