Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Amidophosphoribosyltransferase

Gene

ADE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21NucleophilePROSITE-ProRule annotation
Metal bindingi373 – 3731MagnesiumBy similarity
Metal bindingi374 – 3741MagnesiumBy similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: SGD
  2. identical protein binding Source: IntAct
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.
ReactomeiREACT_301060. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ADE4
Ordered Locus Names:YMR300C
ORF Names:YM9952.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR300c.
SGDiS000004915. ADE4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510AmidophosphoribosyltransferasePRO_0000139647Add
BLAST

Proteomic databases

MaxQBiP04046.
PaxDbiP04046.
PeptideAtlasiP04046.

Expressioni

Gene expression databases

GenevestigatoriP04046.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-14238,EBI-14238

Protein-protein interaction databases

BioGridi35480. 35 interactions.
DIPiDIP-6727N.
IntActiP04046. 9 interactions.
MINTiMINT-625374.
STRINGi4932.YMR300C.

Structurei

3D structure databases

ProteinModelPortaliP04046.
SMRiP04046. Positions 2-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 239238Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
GeneTreeiENSGT00390000003428.
HOGENOMiHOG000033687.
InParanoidiP04046.
KOiK00764.
OMAiGIVTCGP.
OrthoDBiEOG74R20H.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK
60 70 80 90 100
GNGMARDVFT QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI
110 120 130 140 150
NLAHNGNLVN TASLKRYMDE DVHRHINTDS DSELLLNIFA AELEKHNKYR
160 170 180 190 200
VNNEDVFHAL EGVYRLCRGG YACVGLLAGF ALFGFRDPNG IRPLLFGERE
210 220 230 240 250
NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC SKGEPEFKQV
260 270 280 290 300
VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE
310 320 330 340 350
DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV
360 370 380 390 400
SSVRRKLNPM ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA
410 420 430 440 450
SAAPAIRYNH IYGIDLTDTK NLIAYNRTDE EVAEVIGCER VIYQSLEDLI
460 470 480 490 500
DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ ELEEKRESIA NNSSDMKAEV
510
DIGLYNCADY
Length:510
Mass (Da):56,719
Last modified:February 1, 1996 - v2
Checksum:i1FE7A6F5B7C96710
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 472IY → VC in AAA34403 (PubMed:6376509).Curated
Sequence conflicti46 – 472IY → VC in AAA34404 (PubMed:6376509).Curated
Sequence conflicti62 – 621Missing in AAA34403 (PubMed:6376509).Curated
Sequence conflicti62 – 621Missing in AAA34404 (PubMed:6376509).Curated
Sequence conflicti82 – 876GSSANS → PLRLIL (PubMed:6376509).Curated
Sequence conflicti156 – 1583VFH → GFSN in AAA34403 (PubMed:6376509).Curated
Sequence conflicti156 – 1583VFH → GFSN in AAA34404 (PubMed:6376509).Curated
Sequence conflicti234 – 2341V → G in AAA34403 (PubMed:6376509).Curated
Sequence conflicti234 – 2341V → G in AAA34404 (PubMed:6376509).Curated
Sequence conflicti291 – 2911E → Q in AAA34403 (PubMed:6376509).Curated
Sequence conflicti291 – 2911E → Q in AAA34404 (PubMed:6376509).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02203 Genomic DNA. Translation: AAA34403.1.
M57633 Genomic DNA. Translation: AAA34405.1.
M74309 Genomic DNA. Translation: AAA34404.1.
Z49212 Genomic DNA. Translation: CAA89133.1.
BK006946 Genomic DNA. Translation: DAA10201.1.
PIRiS53970.
RefSeqiNP_014029.1. NM_001182809.1.

Genome annotation databases

EnsemblFungiiYMR300C; YMR300C; YMR300C.
GeneIDi855346.
KEGGisce:YMR300C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02203 Genomic DNA. Translation: AAA34403.1.
M57633 Genomic DNA. Translation: AAA34405.1.
M74309 Genomic DNA. Translation: AAA34404.1.
Z49212 Genomic DNA. Translation: CAA89133.1.
BK006946 Genomic DNA. Translation: DAA10201.1.
PIRiS53970.
RefSeqiNP_014029.1. NM_001182809.1.

3D structure databases

ProteinModelPortaliP04046.
SMRiP04046. Positions 2-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35480. 35 interactions.
DIPiDIP-6727N.
IntActiP04046. 9 interactions.
MINTiMINT-625374.
STRINGi4932.YMR300C.

Protein family/group databases

MEROPSiC44.001.

Proteomic databases

MaxQBiP04046.
PaxDbiP04046.
PeptideAtlasiP04046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR300C; YMR300C; YMR300C.
GeneIDi855346.
KEGGisce:YMR300C.

Organism-specific databases

CYGDiYMR300c.
SGDiS000004915. ADE4.

Phylogenomic databases

eggNOGiCOG0034.
GeneTreeiENSGT00390000003428.
HOGENOMiHOG000033687.
InParanoidiP04046.
KOiK00764.
OMAiGIVTCGP.
OrthoDBiEOG74R20H.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.
BioCyciMetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.
ReactomeiREACT_301060. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi979094.
PROiP04046.

Gene expression databases

GenevestigatoriP04046.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase."
    Maentsaelae P., Zalkin H.
    J. Biol. Chem. 259:8478-8484(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Deletion analysis of the 5' flanking region of yeast ADE4 gene."
    Lahti R., Glumoff V., Haemaelaeinen H., Paerssinen R., Maentsaelae P.
    Eur. Congr. Biotechnol. 1:420-423(1986)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUR1_YEAST
AccessioniPrimary (citable) accession number: P04046
Secondary accession number(s): D6W0C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1996
Last modified: April 1, 2015
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.