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P04046

- PUR1_YEAST

UniProt

P04046 - PUR1_YEAST

Protein

Amidophosphoribosyltransferase

Gene

ADE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Metal bindingi373 – 3731MagnesiumBy similarity
    Metal bindingi374 – 3741MagnesiumBy similarity

    GO - Molecular functioni

    1. amidophosphoribosyltransferase activity Source: SGD
    2. identical protein binding Source: IntAct
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: SGD
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YMR300C-MONOMER.
    YEAST:YMR300C-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Gene namesi
    Name:ADE4
    Ordered Locus Names:YMR300C
    ORF Names:YM9952.02C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR300c.
    SGDiS000004915. ADE4.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 510509AmidophosphoribosyltransferasePRO_0000139647Add
    BLAST

    Proteomic databases

    MaxQBiP04046.
    PaxDbiP04046.
    PeptideAtlasiP04046.

    Expressioni

    Gene expression databases

    GenevestigatoriP04046.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-14238,EBI-14238

    Protein-protein interaction databases

    BioGridi35480. 33 interactions.
    DIPiDIP-6727N.
    IntActiP04046. 9 interactions.
    MINTiMINT-625374.
    STRINGi4932.YMR300C.

    Structurei

    3D structure databases

    ProteinModelPortaliP04046.
    SMRiP04046. Positions 2-490.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 239238Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    GeneTreeiENSGT00390000003428.
    HOGENOMiHOG000033687.
    KOiK00764.
    OMAiPITHAHI.
    OrthoDBiEOG74R20H.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00310. GATase_2. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04046-1 [UniParc]FASTAAdd to Basket

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    MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK    50
    GNGMARDVFT QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI 100
    NLAHNGNLVN TASLKRYMDE DVHRHINTDS DSELLLNIFA AELEKHNKYR 150
    VNNEDVFHAL EGVYRLCRGG YACVGLLAGF ALFGFRDPNG IRPLLFGERE 200
    NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC SKGEPEFKQV 250
    VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE 300
    DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV 350
    SSVRRKLNPM ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA 400
    SAAPAIRYNH IYGIDLTDTK NLIAYNRTDE EVAEVIGCER VIYQSLEDLI 450
    DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ ELEEKRESIA NNSSDMKAEV 500
    DIGLYNCADY 510
    Length:510
    Mass (Da):56,719
    Last modified:February 1, 1996 - v2
    Checksum:i1FE7A6F5B7C96710
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 472IY → VC in AAA34403. (PubMed:6376509)Curated
    Sequence conflicti46 – 472IY → VC in AAA34404. (PubMed:6376509)Curated
    Sequence conflicti62 – 621Missing in AAA34403. (PubMed:6376509)Curated
    Sequence conflicti62 – 621Missing in AAA34404. (PubMed:6376509)Curated
    Sequence conflicti82 – 876GSSANS → PLRLIL(PubMed:6376509)Curated
    Sequence conflicti156 – 1583VFH → GFSN in AAA34403. (PubMed:6376509)Curated
    Sequence conflicti156 – 1583VFH → GFSN in AAA34404. (PubMed:6376509)Curated
    Sequence conflicti234 – 2341V → G in AAA34403. (PubMed:6376509)Curated
    Sequence conflicti234 – 2341V → G in AAA34404. (PubMed:6376509)Curated
    Sequence conflicti291 – 2911E → Q in AAA34403. (PubMed:6376509)Curated
    Sequence conflicti291 – 2911E → Q in AAA34404. (PubMed:6376509)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02203 Genomic DNA. Translation: AAA34403.1.
    M57633 Genomic DNA. Translation: AAA34405.1.
    M74309 Genomic DNA. Translation: AAA34404.1.
    Z49212 Genomic DNA. Translation: CAA89133.1.
    BK006946 Genomic DNA. Translation: DAA10201.1.
    PIRiS53970.
    RefSeqiNP_014029.1. NM_001182809.1.

    Genome annotation databases

    EnsemblFungiiYMR300C; YMR300C; YMR300C.
    GeneIDi855346.
    KEGGisce:YMR300C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02203 Genomic DNA. Translation: AAA34403.1 .
    M57633 Genomic DNA. Translation: AAA34405.1 .
    M74309 Genomic DNA. Translation: AAA34404.1 .
    Z49212 Genomic DNA. Translation: CAA89133.1 .
    BK006946 Genomic DNA. Translation: DAA10201.1 .
    PIRi S53970.
    RefSeqi NP_014029.1. NM_001182809.1.

    3D structure databases

    ProteinModelPortali P04046.
    SMRi P04046. Positions 2-490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35480. 33 interactions.
    DIPi DIP-6727N.
    IntActi P04046. 9 interactions.
    MINTi MINT-625374.
    STRINGi 4932.YMR300C.

    Protein family/group databases

    MEROPSi C44.001.

    Proteomic databases

    MaxQBi P04046.
    PaxDbi P04046.
    PeptideAtlasi P04046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR300C ; YMR300C ; YMR300C .
    GeneIDi 855346.
    KEGGi sce:YMR300C.

    Organism-specific databases

    CYGDi YMR300c.
    SGDi S000004915. ADE4.

    Phylogenomic databases

    eggNOGi COG0034.
    GeneTreei ENSGT00390000003428.
    HOGENOMi HOG000033687.
    KOi K00764.
    OMAi PITHAHI.
    OrthoDBi EOG74R20H.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci MetaCyc:YMR300C-MONOMER.
    YEAST:YMR300C-MONOMER.

    Miscellaneous databases

    NextBioi 979094.
    PROi P04046.

    Gene expression databases

    Genevestigatori P04046.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00310. GATase_2. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase."
      Maentsaelae P., Zalkin H.
      J. Biol. Chem. 259:8478-8484(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Deletion analysis of the 5' flanking region of yeast ADE4 gene."
      Lahti R., Glumoff V., Haemaelaeinen H., Paerssinen R., Maentsaelae P.
      Eur. Congr. Biotechnol. 1:420-423(1987)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPUR1_YEAST
    AccessioniPrimary (citable) accession number: P04046
    Secondary accession number(s): D6W0C7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 152 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 18700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3