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Protein

Amidophosphoribosyltransferase

Gene

ADE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 18700 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (ADE4)
  2. Bifunctional purine biosynthetic protein ADE5,7 (ADE5,7)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophilePROSITE-ProRule annotation1
Metal bindingi373MagnesiumBy similarity1
Metal bindingi374MagnesiumBy similarity1

GO - Molecular functioni

  • amidophosphoribosyltransferase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR300C-MONOMER.
YEAST:YMR300C-MONOMER.
ReactomeiR-SCE-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Gene namesi
Name:ADE4
Ordered Locus Names:YMR300C
ORF Names:YM9952.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR300C.
SGDiS000004915. ADE4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001396471 – 510AmidophosphoribosyltransferaseAdd BLAST510

Proteomic databases

MaxQBiP04046.
PRIDEiP04046.

PTM databases

iPTMnetiP04046.

Interactioni

Protein-protein interaction databases

BioGridi35480. 129 interactors.
DIPiDIP-6727N.
IntActiP04046. 8 interactors.
MINTiMINT-625374.
STRINGi4932.YMR300C.

Structurei

3D structure databases

ProteinModelPortaliP04046.
SMRiP04046.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 239Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

GeneTreeiENSGT00390000003428.
HOGENOMiHOG000033687.
InParanoidiP04046.
KOiK00764.
OMAiHVYFARP.
OrthoDBiEOG092C22K6.

Family and domain databases

CDDicd00715. GPATase_N. 1 hit.
cd06223. PRTases_typeI. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
IPR035584. PurF_N.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.

Sequencei

Sequence statusi: Complete.

P04046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK
60 70 80 90 100
GNGMARDVFT QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI
110 120 130 140 150
NLAHNGNLVN TASLKRYMDE DVHRHINTDS DSELLLNIFA AELEKHNKYR
160 170 180 190 200
VNNEDVFHAL EGVYRLCRGG YACVGLLAGF ALFGFRDPNG IRPLLFGERE
210 220 230 240 250
NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC SKGEPEFKQV
260 270 280 290 300
VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE
310 320 330 340 350
DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV
360 370 380 390 400
SSVRRKLNPM ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA
410 420 430 440 450
SAAPAIRYNH IYGIDLTDTK NLIAYNRTDE EVAEVIGCER VIYQSLEDLI
460 470 480 490 500
DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ ELEEKRESIA NNSSDMKAEV
510
DIGLYNCADY
Length:510
Mass (Da):56,719
Last modified:February 1, 1996 - v2
Checksum:i1FE7A6F5B7C96710
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46 – 47IY → VC in AAA34403 (PubMed:6376509).Curated2
Sequence conflicti46 – 47IY → VC in AAA34404 (PubMed:6376509).Curated2
Sequence conflicti62Missing in AAA34403 (PubMed:6376509).Curated1
Sequence conflicti62Missing in AAA34404 (PubMed:6376509).Curated1
Sequence conflicti82 – 87GSSANS → PLRLIL (PubMed:6376509).Curated6
Sequence conflicti156 – 158VFH → GFSN in AAA34403 (PubMed:6376509).Curated3
Sequence conflicti156 – 158VFH → GFSN in AAA34404 (PubMed:6376509).Curated3
Sequence conflicti234V → G in AAA34403 (PubMed:6376509).Curated1
Sequence conflicti234V → G in AAA34404 (PubMed:6376509).Curated1
Sequence conflicti291E → Q in AAA34403 (PubMed:6376509).Curated1
Sequence conflicti291E → Q in AAA34404 (PubMed:6376509).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02203 Genomic DNA. Translation: AAA34403.1.
M57633 Genomic DNA. Translation: AAA34405.1.
M74309 Genomic DNA. Translation: AAA34404.1.
Z49212 Genomic DNA. Translation: CAA89133.1.
BK006946 Genomic DNA. Translation: DAA10201.1.
PIRiS53970.
RefSeqiNP_014029.1. NM_001182809.1.

Genome annotation databases

EnsemblFungiiYMR300C; YMR300C; YMR300C.
GeneIDi855346.
KEGGisce:YMR300C.

Similar proteinsi

Entry informationi

Entry nameiPUR1_YEAST
AccessioniPrimary (citable) accession number: P04046
Secondary accession number(s): D6W0C7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1996
Last modified: October 25, 2017
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names