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P04043

- MTD21_STREE

UniProt

P04043 - MTD21_STREE

Protein

Modification methylase DpnIIA

Gene

dpnM

Organism
Streptococcus pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Nov 1986)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the DpnII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171S-adenosyl-L-methionine
    Binding sitei21 – 211S-adenosyl-L-methionine; via amide nitrogen
    Binding sitei62 – 621S-adenosyl-L-methionine
    Binding sitei194 – 1941S-adenosyl-L-methionine

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3636. M1.DpnII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase DpnIIA (EC:2.1.1.72)
    Short name:
    M.DpnIIA
    Alternative name(s):
    Adenine-specific methyltransferase DpnIIA
    M.DpnII 1
    Gene namesi
    Name:dpnM
    OrganismiStreptococcus pneumoniae
    Taxonomic identifieri1313 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284Modification methylase DpnIIAPRO_0000087957Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    284
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi22 – 243
    Helixi25 – 317
    Beta strandi39 – 413
    Helixi48 – 536
    Beta strandi56 – 638
    Helixi65 – 7612
    Helixi78 – 9114
    Helixi94 – 1018
    Helixi102 – 1054
    Helixi108 – 1114
    Helixi114 – 12714
    Helixi129 – 1313
    Helixi156 – 16813
    Beta strandi169 – 1768
    Helixi178 – 1825
    Beta strandi190 – 1934
    Helixi216 – 23015
    Turni231 – 2333
    Beta strandi235 – 2428
    Helixi244 – 2496
    Turni250 – 2523
    Beta strandi253 – 2586
    Beta strandi276 – 2816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DPMX-ray1.80A1-284[»]
    ProteinModelPortaliP04043.
    SMRiP04043. Positions 10-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04043.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04043-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIKEIKKVT LQPFTKWTGG KRQLLPVIRE LIPKTYNRYF EPFVGGGALF    50
    FDLAPKDAVI NDFNAELINC YQQIKDNPQE LIEILKVHQE YNSKEYYLDL 100
    RSADRDERID MMSEVQRAAR ILYMLRVNFN GLYRVNSKNQ FNVPYGRYKN 150
    PKIVDEELIS AISVYINNNQ LEIKVGDFEK AIVDVRTGDF VYFDPPYIPL 200
    SETSAFTSYT HEGFSFADQV RLRDAFKRLS DTGAYVMLSN SSSALVEELY 250
    KDFNIHYVEA TRTNGAKSSS RGKISEIIVT NYEK 284
    Length:284
    Mass (Da):32,907
    Last modified:November 1, 1986 - v1
    Checksum:i9F1525575CFA3E1E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11226 Genomic DNA. Translation: AAA26872.1.
    M14339 Genomic DNA. Translation: AAA88580.1.
    PIRiA00556. XYSONA.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11226 Genomic DNA. Translation: AAA26872.1 .
    M14339 Genomic DNA. Translation: AAA88580.1 .
    PIRi A00556. XYSONA.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DPM X-ray 1.80 A 1-284 [» ]
    ProteinModelPortali P04043.
    SMRi P04043. Positions 10-284.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3636. M1.DpnII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P04043.

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Dpn II DNA methylase gene of Streptococcus pneumoniae and its relationship to the dam gene of Escherichia coli."
      Mannarelli B.M., Balganesh T.S., Greenberg B., Springhorn S.S., Lacks S.A.
      Proc. Natl. Acad. Sci. U.S.A. 82:4468-4472(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
      Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
      Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease."
      de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.
      J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-14.
    4. "Crystal structure of the DpnM DNA adenine methyltransferase from the DpnII restriction system of Streptococcus pneumoniae bound to S-adenosylmethionine."
      Tran P.H., Korszun Z.R., Cerritelli S., Springhorn S.S., Lacks S.A.
      Structure 6:1563-1575(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 10-284.
      Strain: HB264.

    Entry informationi

    Entry nameiMTD21_STREE
    AccessioniPrimary (citable) accession number: P04043
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: November 1, 1986
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The DpnII restriction system has two different methylases.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3