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Protein

Glutathione peroxidase 1

Gene

Gpx1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protects the hemoglobin in erythrocytes from oxidative breakdown.

Catalytic activityi

2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471
Sitei47 – 471Subject to oxidation and hydroselenide loss to dehydroalanineBy similarity

GO - Molecular functioni

  • glutathione binding Source: RGD
  • glutathione peroxidase activity Source: RGD
  • phospholipid-hydroperoxide glutathione peroxidase activity Source: RGD
  • selenium binding Source: RGD

GO - Biological processi

  • aging Source: RGD
  • glutathione metabolic process Source: RGD
  • hydrogen peroxide catabolic process Source: RGD
  • response to estradiol Source: RGD
  • response to folic acid Source: RGD
  • response to glucose Source: RGD
  • response to hormone Source: RGD
  • response to lipid hydroperoxide Source: RGD
  • response to nicotine Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to oxidative stress Source: RGD
  • response to selenium ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Enzyme and pathway databases

BRENDAi1.11.1.9. 5301.

Protein family/group databases

PeroxiBasei3730. RnoGPx01.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione peroxidase 1 (EC:1.11.1.9)
Short name:
GPx-1
Short name:
GSHPx-1
Alternative name(s):
Cellular glutathione peroxidase
Gene namesi
Name:Gpx1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2729. Gpx1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201Glutathione peroxidase 1PRO_0000066617Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphoserineCombined sources
Modified residuei86 – 861N6-acetyllysine; alternateBy similarity
Modified residuei86 – 861N6-succinyllysine; alternateBy similarity
Modified residuei112 – 1121N6-acetyllysine; alternateBy similarity
Modified residuei112 – 1121N6-succinyllysine; alternateBy similarity
Modified residuei119 – 1191N6-acetyllysineBy similarity
Modified residuei146 – 1461N6-acetyllysine; alternateBy similarity
Modified residuei146 – 1461N6-succinyllysine; alternateBy similarity
Modified residuei195 – 1951PhosphoserineCombined sources
Modified residuei199 – 1991PhosphoserineBy similarity

Post-translational modificationi

During periods of oxidative stress, Sec-47 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04041.
PRIDEiP04041.

PTM databases

iPTMnetiP04041.
PhosphoSiteiP04041.
SwissPalmiP04041.

Expressioni

Tissue specificityi

Expressed in liver and lung.1 Publication

Gene expression databases

BgeeiENSRNOG00000048812.

Interactioni

Subunit structurei

Homotetramer. Interacts with MIEN1 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4564335.
STRINGi10116.ENSRNOP00000066577.

Structurei

3D structure databases

ProteinModelPortaliP04041.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOVERGENiHBG004333.
InParanoidiP04041.
KOiK00432.
PhylomeDBiP04041.

Family and domain databases

CDDicd00340. GSH_Peroxidase. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAARLSAVA QSTVYAFSAR PLAGGEPVSL GSLRGKVLLI ENVASLUGTT
60 70 80 90 100
TRDYTEMNDL QKRLGPRGLV VLGFPCNQFG HQENGKNEEI LNSLKYVRPG
110 120 130 140 150
GGFEPNFTLF EKCEVNGEKA HPLFTFLRNA LPAPSDDPTA LMTDPKYIIW
160 170 180 190 200
SPVCRNDISW NFEKFLVGPD GVPVRRYSRR FRTIDIEPDI EALLSKQPSN

P
Length:201
Mass (Da):22,305
Last modified:February 26, 2008 - v4
Checksum:i093528A214615A11
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 252GG → RE in AAB95647 (PubMed:3408482).Curated
Sequence conflicti154 – 1541C → S in AAB95647 (PubMed:3408482).Curated
Sequence conflicti178 – 1781S → T in AAB95647 (PubMed:3408482).Curated
Sequence conflicti198 – 1981P → S in AAB95647 (PubMed:3408482).Curated

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei47 – 471Selenocysteine1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21210 mRNA. Translation: AAB95647.2.
X07365 mRNA. Translation: CAB43593.1.
X12367 mRNA. Translation: CAA30928.2.
S50336 Genomic DNA. Translation: AAA12407.2.
S41066 mRNA. Translation: AAK72702.1.
AB004231 Genomic DNA. Translation: BAA20399.2.
PIRiA30793. OPRTE.
RefSeqiNP_110453.3. NM_030826.3.
UniGeneiRn.11323.

Genome annotation databases

GeneIDi24404.
KEGGirno:24404.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21210 mRNA. Translation: AAB95647.2.
X07365 mRNA. Translation: CAB43593.1.
X12367 mRNA. Translation: CAA30928.2.
S50336 Genomic DNA. Translation: AAA12407.2.
S41066 mRNA. Translation: AAK72702.1.
AB004231 Genomic DNA. Translation: BAA20399.2.
PIRiA30793. OPRTE.
RefSeqiNP_110453.3. NM_030826.3.
UniGeneiRn.11323.

3D structure databases

ProteinModelPortaliP04041.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4564335.
STRINGi10116.ENSRNOP00000066577.

Protein family/group databases

PeroxiBasei3730. RnoGPx01.

PTM databases

iPTMnetiP04041.
PhosphoSiteiP04041.
SwissPalmiP04041.

Proteomic databases

PaxDbiP04041.
PRIDEiP04041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24404.
KEGGirno:24404.

Organism-specific databases

CTDi2876.
RGDi2729. Gpx1.

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOVERGENiHBG004333.
InParanoidiP04041.
KOiK00432.
PhylomeDBiP04041.

Enzyme and pathway databases

BRENDAi1.11.1.9. 5301.

Miscellaneous databases

PROiP04041.

Gene expression databases

BgeeiENSRNOG00000048812.

Family and domain databases

CDDicd00340. GSH_Peroxidase. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFiPIRSF000303. Glutathion_perox. 1 hit.
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPX1_RAT
AccessioniPrimary (citable) accession number: P04041
Secondary accession number(s): O08946, Q4PIY2, Q91WZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 26, 2008
Last modified: September 7, 2016
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.