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P04039

- COX8_YEAST

UniProt

P04039 - COX8_YEAST

Protein

Cytochrome c oxidase polypeptide VIII, mitochondrial

Gene

COX8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. hydrogen ion transmembrane transport Source: GOC
    2. mitochondrial electron transport, cytochrome c to oxygen Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR395C-MONOMER.
    YEAST:YLR395C-MONOMER.

    Protein family/group databases

    TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase polypeptide VIII, mitochondrial (EC:1.9.3.1)
    Gene namesi
    Name:COX8
    Ordered Locus Names:YLR395C
    ORF Names:L8084.14
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR395c.
    SGDiS000004387. COX8.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex IV Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727Mitochondrion2 PublicationsAdd
    BLAST
    Chaini28 – 7447Cytochrome c oxidase polypeptide VIII, mitochondrialPRO_0000006173Add
    BLAST
    Propeptidei75 – 784PRO_0000006174

    Keywords - PTMi

    Cleavage on pair of basic residues

    Proteomic databases

    MaxQBiP04039.
    PaxDbiP04039.

    Expressioni

    Gene expression databases

    GenevestigatoriP04039.

    Interactioni

    Subunit structurei

    Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

    Protein-protein interaction databases

    BioGridi31654. 89 interactions.
    DIPiDIP-5445N.
    IntActiP04039. 1 interaction.
    MINTiMINT-494943.
    STRINGi4932.YLR395C.

    Structurei

    3D structure databases

    ProteinModelPortaliP04039.
    SMRiP04039. Positions 29-75.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome c oxidase VIIc family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG287990.
    HOGENOMiHOG000001142.
    KOiK02272.
    OMAiKRSSNIM.
    OrthoDBiEOG7M98W2.

    Family and domain databases

    Gene3Di4.10.49.10. 1 hit.
    InterProiIPR004202. COX7C/Cox8.
    [Graphical view]
    PANTHERiPTHR13313. PTHR13313. 1 hit.
    PfamiPF02935. COX7C. 1 hit.
    [Graphical view]
    SUPFAMiSSF81427. SSF81427. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04039-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLCQQMIRTT AKRSSNIMTR PIIMKRSVHF KDGVYENIPF KVKGRKTPYA   50
    LSHFGFFAIG FAVPFVACYV QLKKSGAF 78
    Length:78
    Mass (Da):8,907
    Last modified:February 1, 1996 - v2
    Checksum:i61ABE6001BDABDD0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471T → C AA sequence (PubMed:6327685)Curated
    Sequence conflicti68 – 681C → T AA sequence (PubMed:6327685)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02634 Genomic DNA. Translation: AAA34522.1.
    U19729 Genomic DNA. Translation: AAB82353.1.
    AY558228 Genomic DNA. Translation: AAS56554.1.
    BK006945 Genomic DNA. Translation: DAA09696.1.
    PIRiA25353.
    RefSeqiNP_013499.1. NM_001182283.1.

    Genome annotation databases

    EnsemblFungiiYLR395C; YLR395C; YLR395C.
    GeneIDi851111.
    KEGGisce:YLR395C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02634 Genomic DNA. Translation: AAA34522.1 .
    U19729 Genomic DNA. Translation: AAB82353.1 .
    AY558228 Genomic DNA. Translation: AAS56554.1 .
    BK006945 Genomic DNA. Translation: DAA09696.1 .
    PIRi A25353.
    RefSeqi NP_013499.1. NM_001182283.1.

    3D structure databases

    ProteinModelPortali P04039.
    SMRi P04039. Positions 29-75.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31654. 89 interactions.
    DIPi DIP-5445N.
    IntActi P04039. 1 interaction.
    MINTi MINT-494943.
    STRINGi 4932.YLR395C.

    Protein family/group databases

    TCDBi 3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Proteomic databases

    MaxQBi P04039.
    PaxDbi P04039.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR395C ; YLR395C ; YLR395C .
    GeneIDi 851111.
    KEGGi sce:YLR395C.

    Organism-specific databases

    CYGDi YLR395c.
    SGDi S000004387. COX8.

    Phylogenomic databases

    eggNOGi NOG287990.
    HOGENOMi HOG000001142.
    KOi K02272.
    OMAi KRSSNIM.
    OrthoDBi EOG7M98W2.

    Enzyme and pathway databases

    BioCyci MetaCyc:YLR395C-MONOMER.
    YEAST:YLR395C-MONOMER.

    Miscellaneous databases

    NextBioi 967821.

    Gene expression databases

    Genevestigatori P04039.

    Family and domain databases

    Gene3Di 4.10.49.10. 1 hit.
    InterProi IPR004202. COX7C/Cox8.
    [Graphical view ]
    PANTHERi PTHR13313. PTHR13313. 1 hit.
    Pfami PF02935. COX7C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81427. SSF81427. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "COX8, the structural gene for yeast cytochrome c oxidase subunit VIII. DNA sequence and gene disruption indicate that subunit VIII is required for maximal levels of cellular respiration and is derived from a precursor which is extended at both its NH2 and COOH termini."
      Patterson T.E., Poyton R.O.
      J. Biol. Chem. 261:17192-17197(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The nuclear-coded subunits of yeast cytochrome c oxidase. II. The amino acid sequence of subunit VIII and a model for its disposition in the inner mitochondrial membrane."
      Power S.D., Lochrie M.A., Patterson T.E., Poyton R.O.
      J. Biol. Chem. 259:6571-6574(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-74.
    6. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
      Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
      Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-42, COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCOX8_YEAST
    AccessioniPrimary (citable) accession number: P04039
    Secondary accession number(s): D6VZ30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1986
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 672 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3