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P04039 (COX8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome c oxidase polypeptide VIII, mitochondrial

EC=1.9.3.1
Gene names
Name:COX8
Ordered Locus Names:YLR395C
ORF Names:L8084.14
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length78 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Subunit structure

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Subcellular location

Mitochondrion inner membrane.

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the cytochrome c oxidase VIIc family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Ref.5 Ref.6
Chain28 – 7447Cytochrome c oxidase polypeptide VIII, mitochondrial
PRO_0000006173
Propeptide75 – 784
PRO_0000006174

Experimental info

Sequence conflict471T → C AA sequence Ref.5
Sequence conflict681C → T AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P04039 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 61ABE6001BDABDD0

FASTA788,907
        10         20         30         40         50         60 
MLCQQMIRTT AKRSSNIMTR PIIMKRSVHF KDGVYENIPF KVKGRKTPYA LSHFGFFAIG 

        70 
FAVPFVACYV QLKKSGAF 

« Hide

References

« Hide 'large scale' references
[1]"COX8, the structural gene for yeast cytochrome c oxidase subunit VIII. DNA sequence and gene disruption indicate that subunit VIII is required for maximal levels of cellular respiration and is derived from a precursor which is extended at both its NH2 and COOH termini."
Patterson T.E., Poyton R.O.
J. Biol. Chem. 261:17192-17197(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The nuclear-coded subunits of yeast cytochrome c oxidase. II. The amino acid sequence of subunit VIII and a model for its disposition in the inner mitochondrial membrane."
Power S.D., Lochrie M.A., Patterson T.E., Poyton R.O.
J. Biol. Chem. 259:6571-6574(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-74.
[6]"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42, COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02634 Genomic DNA. Translation: AAA34522.1.
U19729 Genomic DNA. Translation: AAB82353.1.
AY558228 Genomic DNA. Translation: AAS56554.1.
BK006945 Genomic DNA. Translation: DAA09696.1.
PIRA25353.
RefSeqNP_013499.1. NM_001182283.1.

3D structure databases

ProteinModelPortalP04039.
SMRP04039. Positions 29-75.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31654. 89 interactions.
DIPDIP-5445N.
IntActP04039. 1 interaction.
MINTMINT-494943.
STRING4932.YLR395C.

Protein family/group databases

TCDB3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

MaxQBP04039.
PaxDbP04039.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR395C; YLR395C; YLR395C.
GeneID851111.
KEGGsce:YLR395C.

Organism-specific databases

CYGDYLR395c.
SGDS000004387. COX8.

Phylogenomic databases

eggNOGNOG287990.
HOGENOMHOG000001142.
KOK02272.
OMAKRSSNIM.
OrthoDBEOG7M98W2.

Enzyme and pathway databases

BioCycMetaCyc:YLR395C-MONOMER.
YEAST:YLR395C-MONOMER.

Gene expression databases

GenevestigatorP04039.

Family and domain databases

Gene3D4.10.49.10. 1 hit.
InterProIPR004202. COX7C/Cox8.
[Graphical view]
PANTHERPTHR13313. PTHR13313. 1 hit.
PfamPF02935. COX7C. 1 hit.
[Graphical view]
SUPFAMSSF81427. SSF81427. 1 hit.
ProtoNetSearch...

Other

NextBio967821.

Entry information

Entry nameCOX8_YEAST
AccessionPrimary (citable) accession number: P04039
Secondary accession number(s): D6VZ30
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families