P04039 (COX8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase polypeptide VIII, mitochondrial
EC=1.9.3.1

Gene names

Name:	COX8
Ordered Locus Names:	YLR395C
ORF Names:	L8084.14

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subunit structure	Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 672 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cytochrome c oxidase VIIc family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Molecular function	Oxidoreductase
PTM	Cleavage on pair of basic residues
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay PubMed 1331058. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial respiratory chain complex IV Inferred from physical interaction PubMed 1331058. Source: SGD
Molecular_function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion Ref.5 Ref.6
	Chain	28 – 74	47	Cytochrome c oxidase polypeptide VIII, mitochondrial		PRO_0000006173
	Propeptide	75 – 78	4			PRO_0000006174
Experimental info
	Sequence conflict	47	1	T → C AA sequence Ref.5
	Sequence conflict	68	1	C → T AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P04039 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 61ABE6001BDABDD0	FASTA	78	8,907
	10 20 30 40 50 60 MLCQQMIRTT AKRSSNIMTR PIIMKRSVHF KDGVYENIPF KVKGRKTPYA LSHFGFFAIG 70 FAVPFVACYV QLKKSGAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"COX8, the structural gene for yeast cytochrome c oxidase subunit VIII. DNA sequence and gene disruption indicate that subunit VIII is required for maximal levels of cellular respiration and is derived from a precursor which is extended at both its NH2 and COOH termini." Patterson T.E., Poyton R.O. J. Biol. Chem. 261:17192-17197(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The nuclear-coded subunits of yeast cytochrome c oxidase. II. The amino acid sequence of subunit VIII and a model for its disposition in the inner mitochondrial membrane." Power S.D., Lochrie M.A., Patterson T.E., Poyton R.O. J. Biol. Chem. 259:6571-6574(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-74.
[6]	"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method." Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G. Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-42, COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02634 Genomic DNA. Translation: AAA34522.1. U19729 Genomic DNA. Translation: AAB82353.1. AY558228 Genomic DNA. Translation: AAS56554.1. BK006945 Genomic DNA. Translation: DAA09696.1.
PIR	A25353.
RefSeq	NP_013499.1. NM_001182283.1.
3D structure databases
ProteinModelPortal	P04039.
SMR	P04039. Positions 29-75.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5445N.
IntAct	P04039. 1 interaction.
MINT	MINT-494943.
STRING	4932.YLR395C.
Protein family/group databases
TCDB	3.D.4.8.1. proton-translocating cytochrome oxidase (COX) superfamily.
Proteomic databases
PaxDb	P04039.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR395C; YLR395C; YLR395C.
GeneID	851111.
KEGG	sce:YLR395C.
Organism-specific databases
CYGD	YLR395c.
SGD	S000004387. COX8.
Phylogenomic databases
eggNOG	NOG287990.
HOGENOM	HOG000001142.
KO	K02272.
OMA	HFKEGVY.
OrthoDB	EOG4WQ4CB.
Gene expression databases
Genevestigator	P04039.
GermOnline	YLR395C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.49.10. 1 hit.
InterPro	IPR023284. Cyt_c_Oxase_suVIIc_dom. IPR004202. Cyt_c_oxidsae_su7c. [Graphical view]
PANTHER	PTHR13313. PTHR13313. 1 hit.
Pfam	PF02935. COX7C. 1 hit. [Graphical view]
SUPFAM	SSF81427. Cyt_c_Oxase_suVIIc_dom. 1 hit.
ProtoNet	Search...
Other
NextBio	967821.

Entry information

Entry name

COX8_YEAST

Accession

Primary (citable) accession number: P04039
Secondary accession number(s): D6VZ30

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program