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Protein

Cytochrome c oxidase polypeptide VIII, mitochondrial

Gene

COX8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

GO - Molecular functioni

GO - Biological processi

  • hydrogen ion transmembrane transport Source: GOC
  • mitochondrial electron transport, cytochrome c to oxygen Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciMetaCyc:YLR395C-MONOMER.
YEAST:YLR395C-MONOMER.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase polypeptide VIII, mitochondrial (EC:1.9.3.1)
Gene namesi
Name:COX8
Ordered Locus Names:YLR395C
ORF Names:L8084.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XII

Organism-specific databases

CYGDiYLR395c.
EuPathDBiFungiDB:YLR395C.
SGDiS000004387. COX8.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex IV Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727Mitochondrion2 PublicationsAdd
BLAST
Chaini28 – 7447Cytochrome c oxidase polypeptide VIII, mitochondrialPRO_0000006173Add
BLAST
Propeptidei75 – 784PRO_0000006174

Keywords - PTMi

Cleavage on pair of basic residues

Proteomic databases

MaxQBiP04039.
PaxDbiP04039.

Interactioni

Subunit structurei

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Protein-protein interaction databases

BioGridi31654. 89 interactions.
DIPiDIP-5445N.
IntActiP04039. 1 interaction.
MINTiMINT-494943.
STRINGi4932.YLR395C.

Structurei

3D structure databases

ProteinModelPortaliP04039.
SMRiP04039. Positions 29-75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c oxidase VIIc family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG287990.
HOGENOMiHOG000001142.
InParanoidiP04039.
KOiK02272.
OMAiHFKEGVY.
OrthoDBiEOG7M98W2.

Family and domain databases

Gene3Di4.10.49.10. 1 hit.
InterProiIPR004202. COX7C/Cox8.
[Graphical view]
PANTHERiPTHR13313. PTHR13313. 1 hit.
PfamiPF02935. COX7C. 1 hit.
[Graphical view]
SUPFAMiSSF81427. SSF81427. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCQQMIRTT AKRSSNIMTR PIIMKRSVHF KDGVYENIPF KVKGRKTPYA
60 70
LSHFGFFAIG FAVPFVACYV QLKKSGAF
Length:78
Mass (Da):8,907
Last modified:February 1, 1996 - v2
Checksum:i61ABE6001BDABDD0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471T → C AA sequence (PubMed:6327685).Curated
Sequence conflicti68 – 681C → T AA sequence (PubMed:6327685).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02634 Genomic DNA. Translation: AAA34522.1.
U19729 Genomic DNA. Translation: AAB82353.1.
AY558228 Genomic DNA. Translation: AAS56554.1.
BK006945 Genomic DNA. Translation: DAA09696.1.
PIRiA25353.
RefSeqiNP_013499.1. NM_001182283.1.

Genome annotation databases

EnsemblFungiiYLR395C; YLR395C; YLR395C.
GeneIDi851111.
KEGGisce:YLR395C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02634 Genomic DNA. Translation: AAA34522.1.
U19729 Genomic DNA. Translation: AAB82353.1.
AY558228 Genomic DNA. Translation: AAS56554.1.
BK006945 Genomic DNA. Translation: DAA09696.1.
PIRiA25353.
RefSeqiNP_013499.1. NM_001182283.1.

3D structure databases

ProteinModelPortaliP04039.
SMRiP04039. Positions 29-75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31654. 89 interactions.
DIPiDIP-5445N.
IntActiP04039. 1 interaction.
MINTiMINT-494943.
STRINGi4932.YLR395C.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

MaxQBiP04039.
PaxDbiP04039.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR395C; YLR395C; YLR395C.
GeneIDi851111.
KEGGisce:YLR395C.

Organism-specific databases

CYGDiYLR395c.
EuPathDBiFungiDB:YLR395C.
SGDiS000004387. COX8.

Phylogenomic databases

eggNOGiNOG287990.
HOGENOMiHOG000001142.
InParanoidiP04039.
KOiK02272.
OMAiHFKEGVY.
OrthoDBiEOG7M98W2.

Enzyme and pathway databases

BioCyciMetaCyc:YLR395C-MONOMER.
YEAST:YLR395C-MONOMER.

Miscellaneous databases

NextBioi967821.
PROiP04039.

Family and domain databases

Gene3Di4.10.49.10. 1 hit.
InterProiIPR004202. COX7C/Cox8.
[Graphical view]
PANTHERiPTHR13313. PTHR13313. 1 hit.
PfamiPF02935. COX7C. 1 hit.
[Graphical view]
SUPFAMiSSF81427. SSF81427. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "COX8, the structural gene for yeast cytochrome c oxidase subunit VIII. DNA sequence and gene disruption indicate that subunit VIII is required for maximal levels of cellular respiration and is derived from a precursor which is extended at both its NH2 and COOH termini."
    Patterson T.E., Poyton R.O.
    J. Biol. Chem. 261:17192-17197(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The nuclear-coded subunits of yeast cytochrome c oxidase. II. The amino acid sequence of subunit VIII and a model for its disposition in the inner mitochondrial membrane."
    Power S.D., Lochrie M.A., Patterson T.E., Poyton R.O.
    J. Biol. Chem. 259:6571-6574(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-74.
  6. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
    Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
    Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-42, COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCOX8_YEAST
AccessioniPrimary (citable) accession number: P04039
Secondary accession number(s): D6VZ30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.