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Protein

Cytochrome c oxidase subunit 4, mitochondrial

Gene

COX4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi111 – 1111ZincPROSITE-ProRule annotation
Metal bindingi134 – 1341ZincPROSITE-ProRule annotation
Metal bindingi137 – 1371ZincPROSITE-ProRule annotation

GO - Molecular functioni

  • cytochrome-c oxidase activity Source: UniProtKB-EC
  • zinc ion binding Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • hydrogen ion transmembrane transport Source: GOC
  • mitochondrial electron transport, cytochrome c to oxygen Source: SGD
  • mitochondrial respiratory chain complex IV assembly Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YGL187C-MONOMER.
YEAST:YGL187C-MONOMER.
UniPathwayiUPA00705.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 4, mitochondrial (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide IV
Gene namesi
Name:COX4
Ordered Locus Names:YGL187C
ORF Names:G1362
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL187C.
SGDiS000003155. COX4.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: Reactome
  • mitochondrial intermembrane space Source: Reactome
  • mitochondrial respiratory chain complex IV Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525Mitochondrion1 PublicationAdd
BLAST
Chaini26 – 155130Cytochrome c oxidase subunit 4, mitochondrialPRO_0000006114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04037.
PeptideAtlasiP04037.

PTM databases

iPTMnetiP04037.

Interactioni

Subunit structurei

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.1 Publication

Protein-protein interaction databases

BioGridi33068. 145 interactions.
DIPiDIP-2763N.
IntActiP04037. 14 interactions.
MINTiMINT-521387.

Structurei

Secondary structure

1
155
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 173Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi103 – 1064Combined sources
Beta strandi109 – 1146Combined sources
Beta strandi122 – 1243Combined sources
Beta strandi131 – 1333Combined sources
Beta strandi135 – 1373Combined sources
Beta strandi140 – 1434Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HR8X-ray2.70O/P/Q/R2-25[»]
2ODXNMR-A79-155[»]
ProteinModelPortaliP04037.
SMRiP04037. Positions 51-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04037.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000184129.
InParanoidiP04037.
KOiK02265.
OMAiPYWMKVE.
OrthoDBiEOG7BS4NR.

Family and domain databases

Gene3Di2.60.11.10. 1 hit.
InterProiIPR002124. Cyt_c_oxidase_su5b.
IPR020893. Cyt_c_oxidase_su5b_Zn_BS.
[Graphical view]
PANTHERiPTHR10122. PTHR10122. 1 hit.
PfamiPF01215. COX5B. 1 hit.
[Graphical view]
ProDomiPD007270. Cyt_c_oxidase_su5b. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00848. COX5B_1. 1 hit.
PS51359. COX5B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLRQSIRF FKPATRTLCS SRYLLQQKPV VKTAQNLAEV NGPETLIGPG
60 70 80 90 100
AKEGTVPTDL DQETGLARLE LLGKLEGIDV FDTKPLDSSR KGTMKDPIII
110 120 130 140 150
ESYDDYRYVG CTGSPAGSHT IMWLKPTVNE VARCWECGSV YKLNPVGVPN

DDHHH
Length:155
Mass (Da):17,143
Last modified:November 1, 1986 - v1
Checksum:iAB2EE69878819E0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261Q → E AA sequence (PubMed:6327686).Curated
Sequence conflicti73 – 731G → C AA sequence (PubMed:6327686).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01418 Genomic DNA. Translation: CAA25665.1.
X91489 Genomic DNA. Translation: CAA62787.1.
Z72709 Genomic DNA. Translation: CAA96899.1.
EF123139 mRNA. Translation: ABM97483.1.
Y00152 Genomic DNA. Translation: CAA68347.1.
BK006941 Genomic DNA. Translation: DAA07928.1.
PIRiA22786. OLBY4.
RefSeqiNP_011328.1. NM_001181052.1.

Genome annotation databases

EnsemblFungiiYGL187C; YGL187C; YGL187C.
GeneIDi852688.
KEGGisce:YGL187C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01418 Genomic DNA. Translation: CAA25665.1.
X91489 Genomic DNA. Translation: CAA62787.1.
Z72709 Genomic DNA. Translation: CAA96899.1.
EF123139 mRNA. Translation: ABM97483.1.
Y00152 Genomic DNA. Translation: CAA68347.1.
BK006941 Genomic DNA. Translation: DAA07928.1.
PIRiA22786. OLBY4.
RefSeqiNP_011328.1. NM_001181052.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HR8X-ray2.70O/P/Q/R2-25[»]
2ODXNMR-A79-155[»]
ProteinModelPortaliP04037.
SMRiP04037. Positions 51-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33068. 145 interactions.
DIPiDIP-2763N.
IntActiP04037. 14 interactions.
MINTiMINT-521387.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

PTM databases

iPTMnetiP04037.

Proteomic databases

MaxQBiP04037.
PeptideAtlasiP04037.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL187C; YGL187C; YGL187C.
GeneIDi852688.
KEGGisce:YGL187C.

Organism-specific databases

EuPathDBiFungiDB:YGL187C.
SGDiS000003155. COX4.

Phylogenomic databases

HOGENOMiHOG000184129.
InParanoidiP04037.
KOiK02265.
OMAiPYWMKVE.
OrthoDBiEOG7BS4NR.

Enzyme and pathway databases

UniPathwayiUPA00705.
BioCyciMetaCyc:YGL187C-MONOMER.
YEAST:YGL187C-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP04037.
NextBioi972014.
PROiP04037.

Family and domain databases

Gene3Di2.60.11.10. 1 hit.
InterProiIPR002124. Cyt_c_oxidase_su5b.
IPR020893. Cyt_c_oxidase_su5b_Zn_BS.
[Graphical view]
PANTHERiPTHR10122. PTHR10122. 1 hit.
PfamiPF01215. COX5B. 1 hit.
[Graphical view]
ProDomiPD007270. Cyt_c_oxidase_su5b. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00848. COX5B_1. 1 hit.
PS51359. COX5B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein."
    Maarse A.C., van Loon A.P.G.M., Riezman H., Gregor I., Schatz G., Grivell L.A.
    EMBO J. 3:2831-2837(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae."
    Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.
    Yeast 13:55-64(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-33.
    Strain: ATCC 201390 / BY4743.
  6. "The untranslated leader of nuclear COX4 gene of Saccharomyces cerevisiae contains an intron."
    Schneider J.C., Guarente L.
    Nucleic Acids Res. 15:3515-3529(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
  7. "The nuclear-coded subunits of yeast cytochrome c oxidase. III. Identification of homologous subunits in yeast, bovine heart, and Neurospora crassa cytochrome c oxidases."
    Power S.D., Lochrie M.A., Poyton R.O.
    J. Biol. Chem. 259:6575-6578(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-79.
  8. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
    Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
    Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
  9. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  10. "Crystal structures of mitochondrial processing peptidase reveal the mode for specific cleavage of import signal sequences."
    Taylor A.B., Smith B.S., Kitada S., Kojima K., Miyaura H., Otwinowski Z., Ito A., Deisenhofer J.
    Structure 9:615-625(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-25 IN COMPLEX WITH MAS1/MAS2 HETERODIMER.

Entry informationi

Entry nameiCOX4_YEAST
AccessioniPrimary (citable) accession number: P04037
Secondary accession number(s): A2TBN6, D6VTW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: May 11, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9410 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.