Reviewed, UniProtKB/Swiss-Prot P04034 (LDH1_LACLC)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 1
      Short name=L-LDH 1
    EC=1.1.1.27

Gene names

Name:	ldhA
Synonyms:	ldh

Organism

Lactococcus lactis subsp. cremoris (Streptococcus cremoris)

Taxonomic identifier

1359 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer. HAMAP MF_00488
Subcellular location	Cytoplasm. HAMAP MF_00488
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 325	324	L-lactate dehydrogenase 1 HAMAP MF_00488		PRO_0000168354
Regions
	Nucleotide binding	15 – 43	29	NAD By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	123	1	NAD or substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	127	1	I → T in strain: CM1-3.
	Natural variant	213	1	K → E in strain: AM4 and CM1-3.
	Natural variant	293	1	D → E in strain: AM4.
Experimental info
	Sequence conflict	5	1	Q → E AA sequence Ref.3
	Sequence conflict	20	1	S → A AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P04034-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6F50D8343AF13AA9	FASTA	325	35,050
	10 20 30 40 50 60 MADKQRKKVI LVGDGAVGSS YAFALVNQGI AQELGIVDLF KEKTQGDAED LSHALAFTSP 70 80 90 100 110 120 KKIYSADYSD ASDADLVVLT SGAPQKPGET RLDLVEKNLR ITKDVVTKIV ASGFKGIFLV 130 140 150 160 170 180 AANPVDILTY ATWKFSGFPK NRVVGSGTSL DTARFRQALA EKVDVDARSI HAYIMGEHGD 190 200 210 220 230 240 SEFAVWSHAN VAGVKLEQWF QENDYLNEAE IVKLFESVRD AAYSIIAKKG ATFYGVAVAL 250 260 270 280 290 300 ARITKAILDD EHAVLPVSVF QDGQYGVSDC YLGQPAVVGA EGVVNPIHIP LNDAEMQKME 310 320 ASGAQLKAII DEAFAKEEFA SAVKN

References

[1]	"Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes." Swindell S.R., Griffin H.G., Gasson M.J. Microbiology 140:1301-1305(1994) [PubMed: 8081494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ML1.
[2]	"The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes." Urbach E., Daniels B., Salama M.S., Sandine W.E., Giovannoni S.J. Appl. Environ. Microbiol. 63:694-702(1997) [PubMed: 9023947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AM4, AM5, CM1-3 and MSUA2.
[3]	"Partial sequence data for the L-(+)-lactate dehydrogenase from Streptococcus cremoris US3 including the amino acid sequences around the single cysteine residue and at the N-terminus." Crossley L.G., Jago G.R., Davidson B.E. Biochim. Biophys. Acta 581:342-355(1979) [PubMed: 518918] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-22 AND 247-298. Strain: US3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U02385 Genomic DNA. Translation: AAA61961.1. U78630 Genomic DNA. Translation: AAB51674.1. U78631 Genomic DNA. Translation: AAB51675.1. U78633 Genomic DNA. Translation: AAB51677.1. U78635 Genomic DNA. Translation: AAB51679.1.
PIR	A20629.
3D structure databases
HSSP	HSSP built from PDB template 1LLC based on UniProtKB P00343.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.27. 289716.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_LACLC

Accession

Primary (citable) accession number: P04034
Secondary accession number(s): P94885, P94886

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1986
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)