P04007 (RNS2_NICAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease S-2
EC=3.1.27.1

Alternative name(s):
S2-RNase
Stylar glycoprotein 2

Gene names

Name:

S-2

Organism

Nicotiana alata (Winged tobacco) (Persian tobacco)

Taxonomic identifier

4087 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Self-incompatibility (SI) is the inherited ability of a flowering plant to prevent self-fertilization by discriminating between self and non-self pollen during pollination. In many species of the Solanaceae, self-incompatibility is controlled by the single, multiallelic locus S. This stylar glycoprotein is associated with expression of self-incompatibility in potato. Ref.3
Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the RNase T2 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease T2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22
	Chain	23 – 214	192	Ribonuclease S-2		PRO_0000030973
Sites
	Active site	53	1	By similarity
	Active site	109	1	By similarity
	Active site	113	1	By similarity
Amino acid modifications
	Glycosylation	49	1	N-linked (GlcNAc...)		CAR_000106
	Glycosylation	59	1	N-linked (GlcNAc...)		CAR_000107
	Glycosylation	160	1	N-linked (GlcNAc...)		CAR_000108
	Disulfide bond	67 ↔ 116		By similarity
	Disulfide bond	175 ↔ 204		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04007 [UniParc]. Last modified October 23, 1986. Version 1. Checksum: 62343152A6F02C40	FASTA	214	24,876
	10 20 30 40 50 60 MSKSQLTSVF FILLCALSPI YGAFEYMQLV LTWPITFCRI KHCERTPTNF TIHGLWPDNH 70 80 90 100 110 120 TTMLNYCDRS KPYNMFTDGK KKNDLDERWP DLTKTKFDSL DKQAFWKDEY VKHGTCCSDK 130 140 150 160 170 180 FDREQYFDLA MTLRDKFDLL SSLRNHGISR GFSYTVQNLN NTIKAITGGF PNLTCSRLRE 190 200 210 LKEIGICFDE TVKNVIDCPN PKTCKPTNKG VMFP

References

[1]	"Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata." Anderson M.A., Cornish E.C., Mau S.-L., Williams E.G., Hoggart R., Atkinson A., Bonig I., Grego B., Simpson R., Roche P.J., Haley J.D., Penschow J.D., Niall H.D., Tregear G.W., Coghlan J.P., Crawford R.J., Clarke A.E. Nature 321:38-44(1986) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The S-locus of Nicotiana alata: genomic organization and sequence analysis of two S-RNase alleles." Matton D.P., Mau S.L., Okamoto S., Clarke A.E., Newbigin E. Plant Mol. Biol. 28:847-858(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Style self-incompatibility gene products of Nicotiana alata are ribonucleases." McClure B.A., Haring V., Ebert P.R., Anderson M.A., Simpson R.J., Sakiyama F., Clarke A.E. Nature 342:955-957(1989) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03803 mRNA. Translation: CAA27428.1. M24600 mRNA. Translation: AAA34083.1. U08860 Genomic DNA. Translation: AAB40027.1.
PIR	LNNTSA. S57782.
3D structure databases
ProteinModelPortal	P04007.
ModBase	Search...
PTM databases
GlycoSuiteDB	P04007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.90.730.10. 1 hit.
InterPro	IPR001568. RNase_T2-like. IPR018188. RNase_T2_AS. [Graphical view]
PANTHER	PTHR11240. PTHR11240. 1 hit.
Pfam	PF00445. Ribonuclease_T2. 1 hit. [Graphical view]
SUPFAM	SSF55895. RNase_T2. 1 hit.
PROSITE	PS00530. RNASE_T2_1. 1 hit. PS00531. RNASE_T2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNS2_NICAL

Accession

Primary (citable) accession number: P04007

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	October 23, 1986
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program